BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0530.0012.p (59 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1C7G1.1 B1C7G1_9FIRM Putative uncharacterized protein 59 2e-07 >B1C7G1.1 B1C7G1_9FIRM Putative uncharacterized protein Length = 57 Score = 58.9 bits (141), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 25/55 (45%), Positives = 39/55 (70%) Query: 5 KMGRPPSDKPKSKTIEIRVDEETMSKLDASAEKLNTSRSAIVRKGIEKVYDELQK 59 ++GR PS+ PK+ + IR+D+ET+++LD EK N +RS +R GI++ Y EL K Sbjct: 3 RLGRKPSENPKNIMLRIRIDKETLARLDFCCEKENVNRSEFLRNGIDEKYAELNK 57 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.303 0.121 0.305 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 279,127,144 Number of extensions: 6963559 Number of successful extensions: 19299 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 19301 Number of HSP's successfully gapped: 3 Length of query: 59 Length of database: 3,060,696,203 Length adjustment: 31 Effective length of query: 28 Effective length of database: 2,768,644,738 Effective search space: 77522052664 Effective search space used: 77522052664 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 17 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.9 bits) S2: 126 (53.1 bits)