BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0020.p (291 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGI7.1 B3KGI7_9BACI tRNA (guanine-N(1)-)-methyltransferase EC=... 393 e-108 C0ZFN1.1 TRMD_BREBN tRNA (guanine-N(1)-)-methyltransferase EC=2.... 360 6e-98 B1DMF0.1 B1DMF0_9BACL tRNA (guanine-N(1)-)-methyltransferase EC=... 358 3e-97 Q5WFN6.1 TRMD_BACSK tRNA (guanine-N(1)-)-methyltransferase EC=2.... 330 1e-88 A4J665.1 TRMD_DESRM tRNA (guanine-N(1)-)-methyltransferase EC=2.... 298 3e-79 >B3KGI7.1 B3KGI7_9BACI tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 272 Score = 393 bits (1010), Expect = e-108, Method: Compositional matrix adjust. Identities = 205/285 (71%), Positives = 232/285 (81%), Gaps = 18/285 (6%) Query: 2 MRIDVLTLFPEMCEGVFSTSILGKAREKGIVSLNAVNFRDFSGNKHNSVDDTPYGGGGXX 61 MRIDVLTLFPEM +GVF +SILGKA EK IV+LNA+NFR ++GNKH VDDTPYGGGG Sbjct: 1 MRIDVLTLFPEMFDGVFHSSILGKAAEKEIVNLNAINFRQYAGNKHGQVDDTPYGGGG-- 58 Query: 62 XXGGMVLKPDPIFAAVEHVLAASGNLELSRSEGEQAGEAGNVHTIHDDGEAAEYNGESTA 121 GMVLKPDPIFAAVE +LA+ N E+S + GE A AG + AE G A Sbjct: 59 ---GMVLKPDPIFAAVEDILASDPN-EIS-NHGEGAHPAGEI--------LAEITG---A 102 Query: 122 QGKPRIILMCPQGRTYNQQIAEELAQEEHLIFICGHYEGYDERIREHLVTDELSIGDYVL 181 PR+ILMCPQG T+ Q+ AEEL+QEEHL+FICGHYEGYDERIRE LVTDELSIGDYVL Sbjct: 103 AKPPRVILMCPQGETFTQKKAEELSQEEHLVFICGHYEGYDERIREFLVTDELSIGDYVL 162 Query: 182 TGGELPALTVIDSIVRLQPGALGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS 241 TGGELPA+ VIDS+VRL PG LGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS Sbjct: 163 TGGELPAMVVIDSVVRLLPGVLGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS 222 Query: 242 GHHANIEVWRREQALQRTLERRPDLLETVELTAKDKAALKRLKEQ 286 GHHANI+VWRREQAL+RT ERRP+LLE +EL+ +D L++L+ Q Sbjct: 223 GHHANIDVWRREQALKRTWERRPELLEELELSKQDMVYLEKLRAQ 267 >C0ZFN1.1 TRMD_BREBN tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 243 Score = 360 bits (925), Expect = 6e-98, Method: Compositional matrix adjust. Identities = 179/285 (62%), Positives = 208/285 (72%), Gaps = 44/285 (15%) Query: 2 MRIDVLTLFPEMCEGVFSTSILGKAREKGIVSLNAVNFRDFSGNKHNSVDDTPYGGGGXX 61 MRID+ TLFPEM GV S+SILGKA EK +V + NFRDFS +KH +VDDTPYGGGG Sbjct: 1 MRIDIFTLFPEMFTGVLSSSILGKASEKELVDFHVTNFRDFSESKHGTVDDTPYGGGG-- 58 Query: 62 XXGGMVLKPDPIFAAVEHVLAASGNLELSRSEGEQAGEAGNVHTIHDDGEAAEYNGESTA 121 GMVLKP+P+F AVE + T Sbjct: 59 ---GMVLKPEPLFRAVEGL---------------------------------------TG 76 Query: 122 QGKPRIILMCPQGRTYNQQIAEELAQEEHLIFICGHYEGYDERIREHLVTDELSIGDYVL 181 + KPR+ILMCPQG Y+Q++AEELAQEEHL+FICGHYEGYDERIREHLVTDE+SIGDYVL Sbjct: 77 EKKPRVILMCPQGVPYHQKLAEELAQEEHLVFICGHYEGYDERIREHLVTDEISIGDYVL 136 Query: 182 TGGELPALTVIDSIVRLQPGALGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS 241 TGGEL A+ VIDS+VRLQPGALGN+TSAV DSFSTGLLEYPHYTRPAEFRGWKVPD+LLS Sbjct: 137 TGGELAAMVVIDSVVRLQPGALGNQTSAVEDSFSTGLLEYPHYTRPAEFRGWKVPDVLLS 196 Query: 242 GHHANIEVWRREQALQRTLERRPDLLETVELTAKDKAALKRLKEQ 286 GHHANIE WR +++L+RT RRPDLLE + LT + K L L+++ Sbjct: 197 GHHANIESWRLKESLRRTKARRPDLLEKLALTDEMKKLLAELEQE 241 >B1DMF0.1 B1DMF0_9BACL tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 249 Score = 358 bits (920), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 180/289 (62%), Positives = 212/289 (73%), Gaps = 40/289 (13%) Query: 1 MMRIDVLTLFPEMCEGVFSTSILGKAREKGIVSLNAVNFRDFSGNKHNSVDDTPYGGGGX 60 MMRIDVLTLFPEM +GVF SILGKA++KGIVSL A+NFR++S NKHN+VDD PY Sbjct: 1 MMRIDVLTLFPEMFDGVFGASILGKAKDKGIVSLEAINFREYSNNKHNTVDDYPY----- 55 Query: 61 XXXGGMVLKPDPIFAAVEHVLAASGNLELSRSEGEQAGEAGNVHTIHDDGEAAEYNGEST 120 GGMVLKP+P+FAAVE ++A ES Sbjct: 56 GGGGGMVLKPEPVFAAVEDLMA---------------------------------KKESA 82 Query: 121 AQGKPRIILMCPQGRTYNQQIAEELAQEEHLIFICGHYEGYDERIREHLVTDELSIGDYV 180 A +PR+ILMCPQG ++ Q+ AEELA+EEHL+ ICGHYEGYDERIRE+LVTDELSIGDYV Sbjct: 83 A--RPRVILMCPQGESFTQKKAEELAKEEHLVLICGHYEGYDERIREYLVTDELSIGDYV 140 Query: 181 LTGGELPALTVIDSIVRLQPGALGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILL 240 LTGGE+PA+TVIDS+VRL PG LGNETSAVTDS+STGLLEYPHYTRP FR W VPD+L+ Sbjct: 141 LTGGEIPAMTVIDSVVRLLPGVLGNETSAVTDSYSTGLLEYPHYTRPPVFRDWAVPDVLV 200 Query: 241 SGHHANIEVWRREQALQRTLERRPDLLETVELTAKDKAALKRLKEQGSN 289 SG+HA I+ WRREQ+L RTL+RRPDLLE ELT K++ L K+ S Sbjct: 201 SGNHAKIDTWRREQSLLRTLQRRPDLLEKAELTDKERKWLTLQKQDQST 249 >Q5WFN6.1 TRMD_BACSK tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 244 Score = 330 bits (845), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 168/285 (58%), Positives = 200/285 (70%), Gaps = 43/285 (15%) Query: 2 MRIDVLTLFPEMCEGVFSTSILGKAREKGIVSLNAVNFRDFSGNKHNSVDDTPYGGGGXX 61 M+IDVLTLFP+M GVF +SIL +A +KGIVS V+FR+++ NKH VDD PYGGG Sbjct: 1 MKIDVLTLFPDMFTGVFGSSILKQASDKGIVSFQTVDFREYAANKHRKVDDYPYGGG--- 57 Query: 62 XXGGMVLKPDPIFAAVEHVLAASGNLELSRSEGEQAGEAGNVHTIHDDGEAAEYNGESTA 121 GMVL P PIF AV A NG+ Sbjct: 58 --AGMVLSPQPIFDAV---------------------------------SALTKNGKK-- 80 Query: 122 QGKPRIILMCPQGRTYNQQIAEELAQEEHLIFICGHYEGYDERIREHLVTDELSIGDYVL 181 KPRII++CPQG ++QQ A ELA+EEHLIF+CGHYEG+DER+R HLVTDELSIGDYVL Sbjct: 81 --KPRIIMLCPQGEQHSQQKAAELAKEEHLIFLCGHYEGFDERVR-HLVTDELSIGDYVL 137 Query: 182 TGGELPALTVIDSIVRLQPGALGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS 241 TGGEL A+ + DS+VRL PG LGN+ SAVTDS+STGLLE+PHYTRPA+FRG KVPD+LLS Sbjct: 138 TGGELAAMVIADSVVRLLPGVLGNDESAVTDSYSTGLLEHPHYTRPADFRGMKVPDVLLS 197 Query: 242 GHHANIEVWRREQALQRTLERRPDLLETVELTAKDKAALKRLKEQ 286 GHHA IE WR EQ+L+RTLERRPDLL+ E TAK+ LKRL ++ Sbjct: 198 GHHAKIEEWRMEQSLRRTLERRPDLLKGYEPTAKEADILKRLMDK 242 >A4J665.1 TRMD_DESRM tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 254 Score = 298 bits (764), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 144/278 (51%), Positives = 191/278 (68%), Gaps = 41/278 (14%) Query: 2 MRIDVLTLFPEMCEGVFSTSILGKAREKGIVSLNAVNFRDFSGNKHNSVDDTPYGGGGXX 61 MRID+LTLFPEM +G F+ SIL +A+E ++ ++ +N RDFS NKH++VDDTPYGGG Sbjct: 1 MRIDILTLFPEMFQGPFNHSILKRAQENNLLQIDTINIRDFSQNKHHTVDDTPYGGGA-- 58 Query: 62 XXGGMVLKPDPIFAAVEHVLAASGNLELSRSEGEQAGEAGNVHTIHDDGEAAEYNGESTA 121 GMV+ P+P+F +H+ A + AG+ G Sbjct: 59 ---GMVMGPEPLFECFDHLKAKN------------AGQVG-------------------- 83 Query: 122 QGKPRIILMCPQGRTYNQQIAEELAQEEHLIFICGHYEGYDERIREHLVTDELSIGDYVL 181 R+I+MCPQG + Q+ A+ELA+EE+L+ +CGHYEG DER+RE LVTDE+SIGDYVL Sbjct: 84 ----RVIMMCPQGEPFTQEYAKELAREENLVIVCGHYEGIDERVREVLVTDEISIGDYVL 139 Query: 182 TGGELPALTVIDSIVRLQPGALGNETSAVTDSFSTGLLEYPHYTRPAEFRGWKVPDILLS 241 TGGELPA+ V+D++ R+ PG LG SA DSF GLLE+PH+T+P E+RG++VP+ILLS Sbjct: 140 TGGELPAMVVVDAVARMIPGVLGETASAEEDSFYNGLLEHPHFTKPREYRGYEVPEILLS 199 Query: 242 GHHANIEVWRREQALQRTLERRPDLLETVELTAKDKAA 279 GHH NI WRR Q+L RTLERRP+LL+ VEL+ +DK Sbjct: 200 GHHGNIRKWRRRQSLLRTLERRPELLKDVELSKEDKKV 237 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,808,261,890 Number of extensions: 77744620 Number of successful extensions: 179531 Number of sequences better than 1.0e-05: 1470 Number of HSP's gapped: 177920 Number of HSP's successfully gapped: 1995 Length of query: 291 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 157 Effective length of database: 1,798,280,193 Effective search space: 282329990301 Effective search space used: 282329990301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)