BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0012.p (279 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5CID8.1 C5CID8_9THEM Aminotransferase class I and II 304 6e-81 Q8KNS9.1 Q8KNS9_BACTI Putative class-II aminotransferase EC=2.6.1.- 303 1e-80 C3IW29.1 C3IW29_BACTU Class-II aminotransferase 303 1e-80 Q5WDY8.1 Q5WDY8_BACSK Aminotransferase EC=2.6.1.- 299 2e-79 Q8ENY6.1 Q8ENY6_OCEIH Aminotransferase EC=2.6.1.- 280 1e-73 >C5CID8.1 C5CID8_9THEM Aminotransferase class I and II Length = 394 Score = 304 bits (778), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 136/274 (49%), Positives = 197/274 (71%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 VI+Q+PVY+PFY +R N R++ NPLKLE+G Y M++E LE + AR+L+LC+PHNP Sbjct: 115 VIIQTPVYHPFYSTVRENGRQLVKNPLKLENGKYFMNFEDLESKIDERARMLILCNPHNP 174 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 GGRVW ELL+LGELC+++ + ++SDEIH D+ +PG+KHIP AS+S+EL+D T+T +A Sbjct: 175 GGRVWTEGELLKLGELCIKHDLIILSDEIHSDIIMPGYKHIPIASISQELADRTITYIAP 234 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQLI 180 +KTFNL GL TS ++ SN LRR + L +L L++ F A EAAY G EWL+QL+ Sbjct: 235 SKTFNLAGLTTSTVIISNQRLRRIYNNMLSSLELNLGNVFGIVATEAAYRYGEEWLEQLL 294 Query: 181 QHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFNE 240 +++ N E+ +++E LP++K + PE TYL+W++ R LG+D E L+K + +AK+ N+ Sbjct: 295 KYLKGNLEFLKKFISERLPEIKVVEPEGTYLVWLDFRGLGMDQEELRKFIIEKAKLGLND 354 Query: 241 GSIFGTEGQGHLRINLACPRSTLTEALERFARAA 274 G FG EG+G R+N+ACPRS L +ALE+ A Sbjct: 355 GVTFGEEGKGFQRMNIACPRSILKKALEQLETAV 388 >Q8KNS9.1 Q8KNS9_BACTI Putative class-II aminotransferase EC=2.6.1.- Length = 387 Score = 303 bits (775), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 131/272 (48%), Positives = 194/272 (71%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 V++QSPVY+PFYDV+ +R++ N L LEDG Y MD + LE + +++LLC+PHNP Sbjct: 114 VMIQSPVYHPFYDVVLQQNRELVTNNLILEDGRYTMDLKDLENKIDSSVKVMLLCNPHNP 173 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 GRVW+ EL RLG LC ++ + VISDEIHCDL G+KH+PF+S+S+++ ++T ++ Sbjct: 174 VGRVWDEQELERLGALCAKHNIIVISDEIHCDLIYKGNKHVPFSSISEKMKSTSITCIST 233 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQLI 180 +KTFNL GL+T ++ NP ++ +E KLK LSLH +FF AVEAAY G EWL+QL+ Sbjct: 234 SKTFNLAGLKTCNLIIQNPHIKEVYEKKLKMLSLHSESFFGITAVEAAYTHGEEWLNQLL 293 Query: 181 QHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFNE 240 ++ N + + Y +H+ +VK ++PE TYL+W++ R L ++ E ++K MY++AK+A NE Sbjct: 294 IYLEGNLNFLLDYFKQHINEVKVIKPEGTYLVWLDFRGLRMNKENIEKWMYQKAKLALNE 353 Query: 241 GSIFGTEGQGHLRINLACPRSTLTEALERFAR 272 GS+FG G+G LR+N+ACPR+TL E L R + Sbjct: 354 GSMFGENGKGFLRMNIACPRATLEEGLSRIRK 385 >C3IW29.1 C3IW29_BACTU Class-II aminotransferase Length = 387 Score = 303 bits (775), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 131/272 (48%), Positives = 194/272 (71%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 V++QSPVY+PFYDV+ +R++ N L LEDG Y MD + LE + +++LLC+PHNP Sbjct: 114 VMIQSPVYHPFYDVVLQQNRELVTNNLILEDGRYTMDLKDLENKIDSSVKVMLLCNPHNP 173 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 GRVW+ EL RLG LC ++ + VISDEIHCDL G+KH+PF+S+S+++ ++T ++ Sbjct: 174 VGRVWDEQELERLGALCAKHNIIVISDEIHCDLIYKGNKHVPFSSISEKMKSTSITCIST 233 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQLI 180 +KTFNL GL+T ++ NP ++ +E KLK LSLH +FF AVEAAY G EWL+QL+ Sbjct: 234 SKTFNLAGLKTCNLIIQNPHIKEVYEKKLKMLSLHSESFFGITAVEAAYTHGEEWLNQLL 293 Query: 181 QHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFNE 240 ++ N + + Y +H+ +VK ++PE TYL+W++ R L ++ E ++K MY++AK+A NE Sbjct: 294 IYLEGNLNFLLDYFKQHINEVKVIKPEGTYLVWLDFRGLRMNKENIEKWMYQKAKLALNE 353 Query: 241 GSIFGTEGQGHLRINLACPRSTLTEALERFAR 272 GS+FG G+G LR+N+ACPR+TL E L R + Sbjct: 354 GSMFGENGKGFLRMNIACPRATLEEGLSRIRK 385 >Q5WDY8.1 Q5WDY8_BACSK Aminotransferase EC=2.6.1.- Length = 376 Score = 299 bits (766), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 137/273 (50%), Positives = 192/273 (70%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 +I+Q PVYYPFYDVI N R++ NPL + HY MD LE++++ ++LLLC PHNP Sbjct: 98 IIIQPPVYYPFYDVIEKNKRQLVKNPLLFDGNHYKMDLAGLEQVITDKTKMLLLCHPHNP 157 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 GGRVW + EL L E+C R+ + VISDEIH DL G+ H PFA++++ +++ T T LA Sbjct: 158 GGRVWSKEELQALAEICERHNLYVISDEIHADLLFEGYTHTPFATVTETIANRTFTALAP 217 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLHMSAFFAPEAVEAAYNEGGEWLDQLI 180 TKTFNL G+Q +Y++ +P+LR +F +L A ++ S F+ A +AAY G WL +L+ Sbjct: 218 TKTFNLAGVQGAYVIIEDPKLRLEFRRELAATFMNGSNMFSDIATKAAYEHGKPWLKELM 277 Query: 181 QHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFNE 240 +I +N +YA YLA ++P++KP++P+ TYLLW+NC+ELGL P+ KK + +EAKVA N Sbjct: 278 AYIQANYQYAADYLATYMPKIKPIQPQGTYLLWLNCQELGLHPDERKKWLLQEAKVALNH 337 Query: 241 GSIFGTEGQGHLRINLACPRSTLTEALERFARA 273 G+IFG EG+ R+NLA PR TLTE LER RA Sbjct: 338 GAIFGEEGRDFERLNLATPRETLTEGLERIRRA 370 >Q8ENY6.1 Q8ENY6_OCEIH Aminotransferase EC=2.6.1.- Length = 390 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 127/275 (46%), Positives = 178/275 (64%), Gaps = 1/275 (0%) Query: 1 VILQSPVYYPFYDVIRMNDRKVAINPLKLEDGHYVMDYEQLEELMSGGARLLLLCSPHNP 60 +++Q+PVY PFY++I+ DR++ NPL ED +Y +D+ +E+ ++ G + +LCSPHNP Sbjct: 113 ILIQTPVYTPFYNLIKELDREIVKNPLVYEDQYYTIDFNDMEKKLASGIKAFILCSPHNP 172 Query: 61 GGRVWERAELLRLGELCLRYGVTVISDEIHCDLALPGHKHIPFASLSKELSDITLTTLAA 120 GRVW + EL ++ +LCL+Y V + SDEIH DL PG KHIP ASLS+E+SD T+T +A Sbjct: 173 VGRVWTKEELQKMADLCLQYDVMIFSDEIHADLVFPGEKHIPIASLSEEVSDNTITCMAP 232 Query: 121 TKTFNLPGLQTSYIVTSNPELRRKFEYKLKALSLH-MSAFFAPEAVEAAYNEGGEWLDQL 179 +KTFNL GL SY++TSN E R+K + H M A+EAAY G WLD+L Sbjct: 233 SKTFNLAGLDASYVITSNKENRQKLDKAFHRQGFHNMLNTMGNTAMEAAYRHGDYWLDEL 292 Query: 180 IQHISSNAEYAISYLAEHLPQVKPMRPEATYLLWVNCRELGLDPEGLKKLMYREAKVAFN 239 +Q + + +Y H P++K E TYLLWV+C LGL+ + LKK M +A+V N Sbjct: 293 VQVLEQHTQYVREMFEAHAPELKVTHTEGTYLLWVDCSSLGLNKQELKKFMIEKARVGLN 352 Query: 240 EGSIFGTEGQGHLRINLACPRSTLTEALERFARAA 274 G +G EG +RIN+ACPR+TL E +ER A Sbjct: 353 AGQDYGVEGDSFMRINIACPRATLEEGIERIITAV 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,728,286,181 Number of extensions: 69589251 Number of successful extensions: 177234 Number of sequences better than 1.0e-05: 3707 Number of HSP's gapped: 175326 Number of HSP's successfully gapped: 3707 Length of query: 279 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 145 Effective length of database: 1,798,280,193 Effective search space: 260750627985 Effective search space used: 260750627985 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)