BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0008.p (1252 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q06853.1 SLAP2_CLOTH Cell surface glycoprotein 2 133 1e-28 B4BF24.1 B4BF24_CLOTM Cellulosome anchoring protein cohesin region 133 1e-28 B4BF25.1 B4BF25_CLOTM Cellulosome anchoring protein cohesin region 123 1e-25 Q06848.1 ANCA_CLOTH Cellulosome-anchoring protein 123 1e-25 Q6XP42.1 Q6XP42_9FIRM Cellulosomal bifunctional enzyme-binding/s... 122 2e-25 >Q06853.1 SLAP2_CLOTH Cell surface glycoprotein 2 Length = 688 Score = 133 bits (335), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 72/188 (38%), Positives = 103/188 (54%), Gaps = 15/188 (7%) Query: 1076 YMTAPANGLFRPNTAISRYETIAALAPLLDMEE---VNVGNLFNDVTAEHQALTA--FFA 1130 Y+T + +FRP +I+R E A LL E +N + DV + H A A F + Sbjct: 483 YLTGYPDKMFRPEKSITRAEAAVIFAKLLGANENTKINYNVSYTDVDSSHWASWAIKFVS 542 Query: 1131 SAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQS-------DIKGHWSE 1183 + GYPDG F + +TRAEFS T V L V+ G+ ++ D KGHW++ Sbjct: 543 YKKLFTGYPDGSFKPNQNITRAEFS---TVVFKLLVSEKGLKEEKIEKSKFGDTKGHWAQ 599 Query: 1184 KYVNALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ LS GY+ G+PDGTFKP I R+++V LINR G L+ F+D+P +HWA Sbjct: 600 QFIEQLSDLGYINGYPDGTFKPNNNIKRSESVALINRAMGRGPLHGAPQVFEDVPQTHWA 659 Query: 1244 YKDIMSAV 1251 +KDI V Sbjct: 660 FKDIAEGV 667 >B4BF24.1 B4BF24_CLOTM Cellulosome anchoring protein cohesin region Length = 688 Score = 133 bits (335), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 72/188 (38%), Positives = 103/188 (54%), Gaps = 15/188 (7%) Query: 1076 YMTAPANGLFRPNTAISRYETIAALAPLLDMEE---VNVGNLFNDVTAEHQALTA--FFA 1130 Y+T + +FRP +I+R E A LL E +N + DV + H A A F + Sbjct: 483 YLTGYPDKMFRPEKSITRAEAAVIFAKLLGANENTKINYNVSYTDVDSSHWASWAIKFVS 542 Query: 1131 SAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQS-------DIKGHWSE 1183 + GYPDG F + +TRAEFS T V L V+ G+ ++ D KGHW++ Sbjct: 543 YKKLFTGYPDGSFKPNQNITRAEFS---TVVFKLLVSEKGLKEEKIEKSKFGDTKGHWAQ 599 Query: 1184 KYVNALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ LS GY+ G+PDGTFKP I R+++V LINR G L+ F+D+P +HWA Sbjct: 600 QFIEQLSDLGYINGYPDGTFKPNNNIKRSESVALINRAMGRGPLHGAPQVFEDVPQTHWA 659 Query: 1244 YKDIMSAV 1251 +KDI V Sbjct: 660 FKDIAEGV 667 >B4BF25.1 B4BF25_CLOTM Cellulosome anchoring protein cohesin region Length = 447 Score = 123 bits (309), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 68/179 (37%), Positives = 96/179 (53%), Gaps = 10/179 (5%) Query: 1083 GLFRPNTAISRYETIAALAPLLDMEEVNVGN----LFNDVTAEHQALTA--FFASAGIIE 1136 GLF+P I+R E A LL +E + G F D+ H A A + + Sbjct: 249 GLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIKYVTEQNLFG 308 Query: 1137 GYPDGGFGGTKGLTRAEFS----KIMTAVLNLDVTAAGVTKQSDIKGHWSEKYVNALSKA 1192 GYPDG F K +TRAEF+ K + + ++ T+ DI+GHW++KY+ L Sbjct: 309 GYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDIEGHWAQKYIETLVAK 368 Query: 1193 GYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKDIMSAV 1251 GY++G+PD TF+P A I RA++V LINR LN + F D+P ++WAYKDI V Sbjct: 369 GYIKGYPDETFRPQASIKRAESVALINRSLERGPLNGAVLEFTDVPVNYWAYKDIAEGV 427 Score = 57.8 bits (138), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 38/128 (29%), Positives = 61/128 (47%), Gaps = 9/128 (7%) Query: 1132 AGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAG----VTKQSDIKGHWSEKYVN 1187 A ++GYP G F +TRAE + I +L D +AG +T + HW+ + Sbjct: 240 APFLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIK 299 Query: 1188 ALSKAGYVQGFPDGTFKPGAPITRAQAVVL----INRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ G+PDGTF P ITRA+ + + ++ +Q + KD+ HWA Sbjct: 300 YVTEQNLFGGYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDI-EGHWA 358 Query: 1244 YKDIMSAV 1251 K I + V Sbjct: 359 QKYIETLV 366 >Q06848.1 ANCA_CLOTH Cellulosome-anchoring protein Length = 447 Score = 123 bits (309), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 68/179 (37%), Positives = 96/179 (53%), Gaps = 10/179 (5%) Query: 1083 GLFRPNTAISRYETIAALAPLLDMEEVNVGN----LFNDVTAEHQALTA--FFASAGIIE 1136 GLF+P I+R E A LL +E + G F D+ H A A + + Sbjct: 249 GLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIKYVTEQNLFG 308 Query: 1137 GYPDGGFGGTKGLTRAEFS----KIMTAVLNLDVTAAGVTKQSDIKGHWSEKYVNALSKA 1192 GYPDG F K +TRAEF+ K + + ++ T+ DI+GHW++KY+ L Sbjct: 309 GYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDIEGHWAQKYIETLVAK 368 Query: 1193 GYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKDIMSAV 1251 GY++G+PD TF+P A I RA++V LINR LN + F D+P ++WAYKDI V Sbjct: 369 GYIKGYPDETFRPQASIKRAESVALINRSLERGPLNGAVLEFTDVPVNYWAYKDIAEGV 427 Score = 57.8 bits (138), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 38/128 (29%), Positives = 61/128 (47%), Gaps = 9/128 (7%) Query: 1132 AGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAG----VTKQSDIKGHWSEKYVN 1187 A ++GYP G F +TRAE + I +L D +AG +T + HW+ + Sbjct: 240 APFLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIK 299 Query: 1188 ALSKAGYVQGFPDGTFKPGAPITRAQAVVL----INRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ G+PDGTF P ITRA+ + + ++ +Q + KD+ HWA Sbjct: 300 YVTEQNLFGGYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDI-EGHWA 358 Query: 1244 YKDIMSAV 1251 K I + V Sbjct: 359 QKYIETLV 366 >Q6XP42.1 Q6XP42_9FIRM Cellulosomal bifunctional enzyme-binding/scaffoldin-anchoring protein D Length = 852 Score = 122 bits (307), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 64/181 (35%), Positives = 98/181 (54%), Gaps = 5/181 (2%) Query: 1072 GEVK-YMTAPANGLFRPNTAISRYETIAALAPLLDM-EEVNVGNL-FNDVTAEHQALTA- 1127 GE K Y+ + FRP I+R E +A +D+ N N+ F DVT +H A + Sbjct: 651 GEHKSYLKGYTDNCFRPKKEITRAEAAVIIAKFVDVGSSANSNNVNFPDVTNKHWAKESI 710 Query: 1128 -FFASAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQSDIKGHWSEKYV 1186 G+ +GYPDG F + + R EF+ ++ +L+LD + +DI HW++ ++ Sbjct: 711 ELVTKVGLFKGYPDGSFKPDQTIKRGEFAAVIYNLLDLDASTGLSNNFTDIDDHWAKSFI 770 Query: 1187 NALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKD 1246 L+ Y+ G+ DGTFKP I R + VVL+NR LN T+ F D+P ++WA+KD Sbjct: 771 LKLANLRYINGYSDGTFKPDNKIKRDECVVLVNRALNRGPLNGATLDFTDVPKNYWAFKD 830 Query: 1247 I 1247 I Sbjct: 831 I 831 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 6,575,557,159 Number of extensions: 267631107 Number of successful extensions: 539337 Number of sequences better than 1.0e-05: 847 Number of HSP's gapped: 538940 Number of HSP's successfully gapped: 1013 Length of query: 1252 Length of database: 3,060,696,203 Length adjustment: 148 Effective length of query: 1104 Effective length of database: 1,666,385,983 Effective search space: 1839690125232 Effective search space used: 1839690125232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 137 (57.4 bits)