BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0526.0007.p (526 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DEB2.1 B1DEB2_9BACL Flagellar hook-associated protein FlgK 528 e-148 B3K7G3.1 B3K7G3_9BACI Flagellar hook-associated protein FlgK 518 e-145 A6TL84.1 A6TL84_ALKMQ Flagellar hook-associated protein FlgK 301 1e-79 A4ISV3.1 A4ISV3_GEOTN Flagellar hook-associated protein 1 296 5e-78 Q5KV64.1 Q5KV64_GEOKA Flagellar hook-associated protein 1 (HAP1) 290 2e-76 >B1DEB2.1 B1DEB2_9BACL Flagellar hook-associated protein FlgK Length = 518 Score = 528 bits (1360), Expect = e-148, Method: Compositional matrix adjust. Identities = 276/531 (51%), Positives = 368/531 (69%), Gaps = 18/531 (3%) Query: 1 MTSTFHSIETAKRSLNTQTAALNTAGHNIANVNTEGYSRQVVKMQASLPMEAYGFLRSTA 60 MTSTFH +ETAKRS+ T ALNT GHNI+N NT GYSRQ V A+ P+EAYG+ STA Sbjct: 1 MTSTFHGLETAKRSIFTTQTALNTTGHNISNANTSGYSRQRVNFTAARPIEAYGYSHSTA 60 Query: 61 AGQMGTGVDFTSIERVRQSFLDDQYRNESSNSGSWDVRFDTLKKLESIVNEPSDTGIRKV 120 AGQ+GTGV+ +SI RVR FLD Q+ +E G++ V+ DTL+KLE+IVNEPSDTG++ V Sbjct: 61 AGQLGTGVEASSITRVRDKFLDSQFYSEYKWLGNYTVQSDTLEKLETIVNEPSDTGLQTV 120 Query: 121 LDNFWNAWSDLSQDPENITNRKIVKETTKALTDAMNQVSTQLDALTTDLTSNISLKTTEI 180 + NFWN+WSDLS+ PE+ R ++ E TKA+ DA N S QL L +DLT N + + Sbjct: 121 ISNFWNSWSDLSKTPESADGRAVLVEQTKAMMDAFNYTSKQLADLKSDLTENAQINLDNL 180 Query: 181 NSLLQTVADLNNNITKLEGLGDNPNDLRDQRDLAIDKLSKLANVQVTQLSDGYQVTLSGQ 240 S+ T+A+LN I ++EG+GD+ NDLRDQRD +D+LS LAN++V+ LSDGY +T+ Sbjct: 181 KSISSTIANLNGQIQRIEGIGDDANDLRDQRDALVDELSGLANIKVSDLSDGYTITMGNV 240 Query: 241 LVVTGDQV---TAVTADGLKAAYDGGTLTGGEVYGMFMSRDKYVTDYKNQMNQLANTLAN 297 +V G +T D + AA + G L G +YG SRDKYV Y +Q+NQ+ANT+AN Sbjct: 241 SLVAGKSAEGSQGLTLDDMTAAIENGDLNNGAIYGTMQSRDKYVQGYMDQLNQMANTIAN 300 Query: 298 GDIEITLPAGTVLPEGTVLNTGANGTPVTYSNANNNRTITADTKVTVKGINGLLQLGYTV 357 GDI++T+P G+VLPEGTVLN VTY+ A NRT+ D VTV+G+NGL QLGYT+ Sbjct: 301 GDIQVTIPKGSVLPEGTVLN------GVTYAGA--NRTLAEDLTVTVQGLNGLHQLGYTM 352 Query: 358 SGATPEKGLALFTTKDSGA--ITAGNITLNPLIAADPNKISTSMRVTGVGTTNEKVVIGN 415 +G E+G LFT + +TA NI LNP IAADP ++TSM+VT E VV GN Sbjct: 353 NG---EQGEPLFTDANGNTDNLTAANIQLNPKIAADPTLLATSMKVTFNDDGTETVVAGN 409 Query: 416 NDMALIMAGLKTAKFDFGSALSAPGTMDDYFASIVGQLGVQTQEAERQAKNAEQLTEQVE 475 N MA++M+ + KF F + + T+ +F ++VGQLGVQ++E +R N + + +QV+ Sbjct: 410 NSMAVLMSQARDTKFAFDA--TDNNTISGFFRAVVGQLGVQSKEMQRLQTNQQTIVDQVD 467 Query: 476 LNRASVSGVSLDEEMTDLIKFQHAYSAASRFMTTFDELLNKLINGTGRVGL 526 R SVSGVSLDEEM+DL+KFQHAY+A++R MT DE L+++IN GRVGL Sbjct: 468 SRRMSVSGVSLDEEMSDLVKFQHAYNASARVMTVIDETLDRIINSMGRVGL 518 >B3K7G3.1 B3K7G3_9BACI Flagellar hook-associated protein FlgK Length = 520 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 276/529 (52%), Positives = 362/529 (68%), Gaps = 14/529 (2%) Query: 1 MTSTFHSIETAKRSLNTQTAALNTAGHNIANVNTEGYSRQVVKMQASLPMEAYGFLRSTA 60 M STFH IETAKRSL Q A +NT GHNIAN +T GYSRQVV + AS P+ G + Sbjct: 1 MVSTFHGIETAKRSLFAQMAGMNTTGHNIANASTPGYSRQVVGLIASRPLAMPGMYNQIS 60 Query: 61 AGQMGTGVDFTSIERVRQSFLDDQYRNESSNSGSWDVRFDTLKKLESIVNEPSDTGIRKV 120 GQ+GTGV+ I+RVR FLD QY N + + G W+V+ TL KLE+I NEPS++G+ V Sbjct: 61 PGQLGTGVEVNYIKRVRDQFLDQQYWNANRSLGDWEVQAGTLSKLETIFNEPSESGLTTV 120 Query: 121 LDNFWNAWSDLSQDPENITNRKIVKETTKALTDAMNQVSTQLDALTTDLTSNISLKTTEI 180 +DNFW AWS+ S+DPEN+T+RKIV+E A+ +A N+ S ++ + DLT I++ I Sbjct: 121 IDNFWKAWSEFSKDPENVTSRKIVRENALAMAEAFNETSKKITDMQADLTQGIAVNADHI 180 Query: 181 NSLLQTVADLNNNITKLEGLGDNPNDLRDQRDLAIDKLSKLANVQVTQLSDGYQVTLSGQ 240 N+L VA LN I ++EG DN NDLRDQRDL ID+LS++ANV V + GY+VT+ G Sbjct: 181 NTLTTQVAQLNYEINRIEGFRDNANDLRDQRDLIIDELSRMANVTVVEEETGYRVTVGGL 240 Query: 241 LVVTGDQVTAVTADGLKAAYDGGTLTGGEVYGMFMSRDKYVTDYKNQMNQLANTLANGDI 300 +V GD VT L+ AY G TL GE++G+ SRD V DY +Q+N+LANT+ANGD+ Sbjct: 241 ELVAGDAAVPVTPAQLETAYAGNTLNSGELFGIIKSRDGIVKDYLDQLNELANTIANGDV 300 Query: 301 EITLPAGTVLPEGTVLNTGANGTPVTYSNANNNRTITADTKVTVKGINGLLQLGYTVSGA 360 IT+PAG+VLPEGTVLN VTYS RT+TADT VTVKG+NGL +LGY + Sbjct: 301 SITIPAGSVLPEGTVLNG------VTYSGT--TRTLTADTTVTVKGLNGLHKLGYLFTSP 352 Query: 361 TPEKGLALFTTKDSGAITAGNITLNPLIAADPNKISTSMRVTGVGTTNEKVVIGNNDMAL 420 G F++KD G ITA + ++N I DPNKI++S+RV GT ++VV GNN +AL Sbjct: 353 AQAAG-DFFSSKDGGPITAASFSVNNAIVQDPNKIASSLRVVA-GTNGDEVVKGNNTLAL 410 Query: 421 IMAGLKTAKFDFGSALS----APGTMDDYFASIVGQLGVQTQEAERQAKNAEQLTEQVEL 476 +M+ L+ F F + S GT+DDY+ +++GQLGVQ++EA RQ N + L +QV+ Sbjct: 411 LMSQLRERPFTFNAIASNNGITTGTVDDYYRAVMGQLGVQSREALRQQDNNQLLVDQVDS 470 Query: 477 NRASVSGVSLDEEMTDLIKFQHAYSAASRFMTTFDELLNKLINGTGRVG 525 R SVSGVSLDEEM++LIK QH+Y AASRFMTT D+ L+K+IN G VG Sbjct: 471 RRQSVSGVSLDEEMSNLIKLQHSYGAASRFMTTVDQNLDKIINSMGVVG 519 >A6TL84.1 A6TL84_ALKMQ Flagellar hook-associated protein FlgK Length = 493 Score = 301 bits (770), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 197/537 (36%), Positives = 291/537 (54%), Gaps = 57/537 (10%) Query: 1 MTSTFHSIETAKRSLNTQTAALNTAGHNIANVNTEGYSRQVVKMQASLPMEAYGFLRSTA 60 M STF TA + +L+ GHNIAN NT GYSRQ ++ AS PM YG Sbjct: 1 MRSTFSGFNTATSGIFASQRSLDVVGHNIANANTPGYSRQRLEQAASTPMSLYG-----G 55 Query: 61 AGQMGTGVDFTSIERVRQSFLDDQYRNESSNSGSWDVRFDTLKKLESIVNEPSDTGIRKV 120 G +GTGVD +I+++R FLD +YR+E + G W+ + L+ +ESI NEPSDT + V Sbjct: 56 KGMLGTGVDTVAIKQMRSEFLDFKYRDEVNALGYWETKEYGLEFIESIFNEPSDTSVATV 115 Query: 121 LDNFWNAWSDLSQDPENITNRKIVKETTKALTDAMNQVSTQLDALTTDLTSNISLKTTEI 180 D + + +LS++PE+IT R +V+E A T+ MNQ QL+ L D+ S+I+ + I Sbjct: 116 TDELFAGFEELSKNPESITARTVVRERGIAFTNTMNQTYKQLEKLAKDVNSDINATVSSI 175 Query: 181 NSLLQTVADLNNNITKLEGLGDNPNDLRDQRDLAIDKLSKLANVQVTQLSDGYQ-----V 235 NS +A+LN I + E G + NDLRD+R+L ID LSKL NV+V ++DGY V Sbjct: 176 NSYADQIAELNKQIYRFEADGSSANDLRDRRNLLIDDLSKLVNVEVRDVNDGYGSSKMVV 235 Query: 236 TLSGQLVVTGDQVTAVTADGLKAAYDGGTLTGGEVYGMFMSRDKYVTDYKNQMNQLANTL 295 ++GQ +V DQ + A K+ + + +++N Sbjct: 236 QINGQPLVNHDQAYKLDATATKS-----------------------STFDDEINMTEIKW 272 Query: 296 ANGDI------EITLPAGTVLPEGTVLNTGANGTPVTYSNANNNRTITADTKVTVKGING 349 ANG + + L A L +GT + G P N+ A K +NG Sbjct: 273 ANGSVVNQDSLKGELGALLTLRDGTTKDN--KGIPFYIEQLNDFVGSFA------KAVNG 324 Query: 350 LLQLGYTVSGATPEKGLALFTTKDSG-AITAGNITLNPLIAADPNKISTSMRVTGVGTTN 408 + + G + G ++G+A FT ++G ITA NI ++ I DP KI+ + +G Sbjct: 325 IHEDGLDLDG---DEGVAFFTDGNTGDQITAKNIQISLEIDRDPRKIAAAT----MGELG 377 Query: 409 EKVVIGNNDMALIMAGLKTAKFDFGSALSAPGTMDDYFASIVGQLGVQTQEAERQAKNAE 468 E VV G+ AL + L+ A+ F + GT +D+ S++ LGV TQEA R A N Sbjct: 378 EPVV-GDGSNALRLTELRHAQMSFDNG-EFKGTPEDFMISLIANLGVNTQEATRMASNQS 435 Query: 469 QLTEQVELNRASVSGVSLDEEMTDLIKFQHAYSAASRFMTTFDELLNKLINGTGRVG 525 LT Q++ R S+SGVSLDEEM ++++FQHAY+A++R +TT DE+++ +IN G VG Sbjct: 436 ILTNQIDRQRQSISGVSLDEEMANMVRFQHAYNASARMVTTMDEMIDVIINRMGTVG 492 >A4ISV3.1 A4ISV3_GEOTN Flagellar hook-associated protein 1 Length = 540 Score = 296 bits (757), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 199/548 (36%), Positives = 289/548 (52%), Gaps = 32/548 (5%) Query: 1 MTSTFHSIETAKRSLNTQTAALNTAGHNIANVNTEGYSRQVVKMQASLPMEAYGFLRSTA 60 M STFH +E A+R++ Q AAL T GHNIAN NTEGY+RQ V+++A+ P G R Sbjct: 1 MLSTFHGLEAARRAMMAQQAALYTTGHNIANANTEGYTRQRVQLKATPPFPTPGLNRPNI 60 Query: 61 AGQMGTGVDFTSIERVRQSFLDDQYRNESSNSGSWDVRFDTLKKLESIVNEPSDTGIRKV 120 GQMGTGV+ S+ERVR+ FLD QYR E+S G W+ R D + K+E I+NEPSD+G+ K Sbjct: 61 PGQMGTGVEAASVERVREYFLDIQYRGENSKLGYWEARADAVSKMEDIMNEPSDSGLAKT 120 Query: 121 LDNFWNAWSDLSQDPENITNRKIVKETTKALTDAMNQVSTQLDALTTDLTSNISLKTTEI 180 +D FW A DLS PEN R +V++ A+ + + +S L + TD+ + I + T+I Sbjct: 121 MDQFWQALQDLSTHPENEGARSVVRQRGLAVVETFHYLSNSLSQIQTDIGTQIGVTVTQI 180 Query: 181 NSLLQTVADLNNNITKLEGLGDNPNDLRDQRDLAIDKLSKLANVQVTQLSDGYQVTLSGQ 240 NSL + +++LN I +E G PNDL D+RD ID+LSKL NVQV + G + + Sbjct: 181 NSLAKQISELNKQIASVEPNGYLPNDLYDERDRLIDELSKLINVQVEKHPTGGNAPATAE 240 Query: 241 -----LVVTGDQVTAVTADGLKAAY---DGGTLTGGEVYGMFMSRDKYVTDYKNQMNQLA 292 + G++ + G + DG + G + + M VT+ + ++ Sbjct: 241 GTYDIYFLNGNEKVYLVQGGEFQSISFPDGQDVDGDKEHIKEMPPATGVTELQVGGTSVS 300 Query: 293 NTLANGDIEITLPAGT----VLPEGTVLNTGANGTPVT---YSNANNNRTITADTKVTVK 345 T N ++T P G + G V ++G P+ Y + NN A T K Sbjct: 301 FTSNN---QVTFPMGKLRGLIEAYGYVSGQDSSGQPIVTGIYPDMLNNLDKLAYT--FGK 355 Query: 346 GINGLLQLGYTVSGATPEKGLALFTTKDSGAITAGNITLNPLIAADPNKISTSMRVTGVG 405 N + + GY ++G E G++ F A A D I+ S G Sbjct: 356 LFNAVHEKGYGLNG---ETGVSFFDGLGQEAKGAAKTIRLSADIDDLANIAASTEEGKPG 412 Query: 406 TTNEKVVIGN--------NDMALIMAGLKTAKFDFGSALSAPGTMDDYFASIVGQLGVQT 457 N + + N N ++LI D L++ GT+ + +G+LGV Sbjct: 413 NGNNAINLANVGSMLLSSNTISLIGTSATIQMSDLQLPLTS-GTIQTNYQGWIGKLGVDG 471 Query: 458 QEAERQAKNAEQLTEQVELNRASVSGVSLDEEMTDLIKFQHAYSAASRFMTTFDELLNKL 517 Q+A R N++ L + VE R SVS VSLDEEM ++IKFQHAY+AA+R +T DE+L+K+ Sbjct: 472 QQANRMKNNSDVLRQSVEERRQSVSSVSLDEEMMNMIKFQHAYNAAARQITVVDEMLDKI 531 Query: 518 INGTGRVG 525 ING G VG Sbjct: 532 INGMGIVG 539 >Q5KV64.1 Q5KV64_GEOKA Flagellar hook-associated protein 1 (HAP1) Length = 531 Score = 290 bits (743), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 203/564 (35%), Positives = 296/564 (52%), Gaps = 73/564 (12%) Query: 1 MTSTFHSIETAKRSLNTQTAALNTAGHNIANVNTEGYSRQVVKMQASLPMEAYGFLRSTA 60 M STFH +E A+R++ Q AAL T GHNIAN NTEGY+RQ V+++A+ P G R Sbjct: 1 MLSTFHGLEAARRAMMAQQAALYTTGHNIANANTEGYTRQRVQLKATPPFPTPGLNRPNI 60 Query: 61 AGQMGTGVDFTSIERVRQSFLDDQYRNESSNSGSWDVRFDTLKKLESIVNEPSDTGIRKV 120 GQMGTGV+ +S+ERVR+ FLD QYR E+S G W+ R D + K+E I+NEPSD+G+ K Sbjct: 61 PGQMGTGVEASSVERVREYFLDIQYRGENSKLGYWEARADAVSKMEDIMNEPSDSGLAKT 120 Query: 121 LDNFWNAWSDLSQDPENITNRKIVKETTKALTDAMNQVSTQLDALTTDLTSNISLKTTEI 180 +D FW A DLS PEN R +V++ A+ + + +S L + TDL + I TEI Sbjct: 121 MDQFWQALQDLSTHPENEGARSVVRQRGLAVVETFHYLSNSLAQIRTDLGTQIGTTVTEI 180 Query: 181 NSLLQTVADLNNNITKLEGLGDNPNDLRDQRDLAIDKLSKLANVQVTQLSDGYQVTLSGQ 240 NSLL ++ LN I ++E G PNDL D+RD ID+LS+ + V + G Sbjct: 181 NSLLNQISALNERIAEIEPNGYLPNDLYDERDRLIDQLSQYMEIGVEKHPTG-------- 232 Query: 241 LVVTGDQVTAVTADGLKAAYDGGTLTGGEVYGMFMSRDKYVTDYK-------NQMNQLAN 293 G+ + + YD + G + R V + + + ++ Sbjct: 233 ----GNALPMA-----EGTYDVYVMNGSAKVYLVQGRTASVVSFSDGSDVDGDGVKEMPP 283 Query: 294 TLANGDIEI--------TLPAGTV--LPEGTVLNTG--ANGTPVT---YSN--ANNNRTI 336 L +++ +LP G + L EG TG A+G P+ Y + AN ++ Sbjct: 284 DLGINTLDVSGTTVSFSSLPNGKLRGLIEGYGYQTGTDASGQPIVKGLYPDMLANLDKLA 343 Query: 337 TADTKVTVKGINGLLQLGYTVSGATPEKGLALFTTKDSGAITAGNITLNPLIAADPNKIS 396 KV N + Q GY ++G+T F GA++ T+ +AAD + ++ Sbjct: 344 YTFGKV----FNDVHQQGYGLNGSTNHP---FF--DGIGAVSGAAKTIK--LAADIDDLA 392 Query: 397 TSMRVTGVGTTNEKVVIGNNDMALIMAGLK-------TAKFDFG-----SALSAP---GT 441 T G T GN + A+ +A ++ T G ++L+ P GT Sbjct: 393 NIAASTKSGET------GNGNNAINLANVRSLLLSNATVSLQDGISVNLASLNLPLSSGT 446 Query: 442 MDDYFASIVGQLGVQTQEAERQAKNAEQLTEQVELNRASVSGVSLDEEMTDLIKFQHAYS 501 ++ + +GQLGV ++A R N+E L + VE R SVS VSLDEEM ++IKFQHAY+ Sbjct: 447 VETNYQGWIGQLGVDGEQANRMKSNSEILRQSVEEKRQSVSSVSLDEEMMNMIKFQHAYN 506 Query: 502 AASRFMTTFDELLNKLINGTGRVG 525 AA+R +T DE+L+KLING G VG Sbjct: 507 AAARQITVIDEMLDKLINGMGVVG 530 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.311 0.127 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,871,639,570 Number of extensions: 117774201 Number of successful extensions: 352311 Number of sequences better than 1.0e-05: 901 Number of HSP's gapped: 350558 Number of HSP's successfully gapped: 1236 Length of query: 526 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 385 Effective length of database: 1,732,333,088 Effective search space: 666948238880 Effective search space used: 666948238880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 134 (56.2 bits)