BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0056.p (298 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D896.1 B1D896_9BACL Phosphate ABC transporter, inner membrane ... 350 8e-95 C0ZK03.1 C0ZK03_BREBN Probable phosphate ABC transporter permeas... 304 7e-81 B3WCN7.1 B3WCN7_LACCB Phosphate ABC transporter, membrane-spanni... 303 2e-80 Q65HB8.1 Q65HB8_BACLD PstA 302 2e-80 A0ALI1.1 A0ALI1_LISW6 Complete genome 300 1e-79 >B1D896.1 B1D896_9BACL Phosphate ABC transporter, inner membrane subunit PstA Length = 295 Score = 350 bits (899), Expect = 8e-95, Method: Compositional matrix adjust. Identities = 174/295 (58%), Positives = 208/295 (70%) Query: 1 LKPRTADKIATXXXXXXXXXXXXXXXXXXXXXXXXXXNHISWDFLTSAPQKIRAGGGVGP 60 + +T DKIAT HISW FLTS P+ IR GGG+GP Sbjct: 1 MSAKTVDKIATGIIIFITGLIALALLGLLGYILFRGLGHISWHFLTSPPETIRKGGGIGP 60 Query: 61 QLFNSIYXXXXXXXXXXXXXXXAGIFMAEYARPGKLTNFIRLIVEVLSSFPSXXXXXXXX 120 QLFNS++ AGI+M+EYA+ GK+T+ IRL+VEVLSSFPS Sbjct: 61 QLFNSLFLLVLTMIITVPLGIGAGIYMSEYAKAGKITSAIRLVVEVLSSFPSIIVGLFGL 120 Query: 121 XXXXNTFDLGFSLVSGALALTFFNLPLMVRITEQAFRTVPKQQKEAGFALGLSKWKIVTN 180 N F+LGFSL+SGA+ALT FNLPLMVRITEQ R+VP QQKEA ALG+SKWK + + Sbjct: 121 LVIVNKFNLGFSLLSGAIALTIFNLPLMVRITEQGLRSVPLQQKEASLALGISKWKTIRS 180 Query: 181 VLFPVALPTIITGTILSAGRVFGEAAALMFTAGMSSPRLDFSNWNPSSPTSPLNPFRPAE 240 V P+ALP +++G+IL+AGR+FGEAAAL+FTAGMSSPRLDFS+WNP SPLNP RPAE Sbjct: 181 VQLPIALPIVLSGSILAAGRIFGEAAALLFTAGMSSPRLDFSDWNPLHTASPLNPMRPAE 240 Query: 241 TLAVHIWKINSEGLAPDAAQIAAGASAVLVITVLIFNLAARYFGRVIYRKLTASK 295 TLAVHIWK+NSEGLAPDA QIAAGASAVL+ITVL+FNL AR GR ++ + TASK Sbjct: 241 TLAVHIWKVNSEGLAPDATQIAAGASAVLIITVLLFNLGARALGRYMHNRFTASK 295 >C0ZK03.1 C0ZK03_BREBN Probable phosphate ABC transporter permease protein Length = 296 Score = 304 bits (778), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 152/296 (51%), Positives = 195/296 (65%) Query: 1 LKPRTADKIATXXXXXXXXXXXXXXXXXXXXXXXXXXNHISWDFLTSAPQKIRAGGGVGP 60 +K R D+ AT + ++ DFLTS P + AGGG+GP Sbjct: 1 MKARLMDRAATVVLTLIAVLIIGTLVGLLGFILSQGWHKLNLDFLTSPPDIVNAGGGIGP 60 Query: 61 QLFNSIYXXXXXXXXXXXXXXXAGIFMAEYARPGKLTNFIRLIVEVLSSFPSXXXXXXXX 120 QLFNS+Y AGI+MAEYA K+T FIR+ +EVLSS PS Sbjct: 61 QLFNSLYLLVLTMLIALPLGIGAGIYMAEYAPDNKITQFIRMSIEVLSSLPSIVVGLFGL 120 Query: 121 XXXXNTFDLGFSLVSGALALTFFNLPLMVRITEQAFRTVPKQQKEAGFALGLSKWKIVTN 180 N G++L GALALT FNLPL+VR+TE A R VP+ QKEA ALG++KW+ + + Sbjct: 121 LVFVNMTGWGYTLFGGALALTVFNLPLLVRVTEDALRNVPRSQKEASLALGITKWRTIVS 180 Query: 181 VLFPVALPTIITGTILSAGRVFGEAAALMFTAGMSSPRLDFSNWNPSSPTSPLNPFRPAE 240 V+ P ALP I+TG IL++GRVFGEAAAL+FTAGMSSP LDF++++ +S TSPLNPFRPAE Sbjct: 181 VILPAALPGILTGAILASGRVFGEAAALLFTAGMSSPNLDFTDFSMNSTTSPLNPFRPAE 240 Query: 241 TLAVHIWKINSEGLAPDAAQIAAGASAVLVITVLIFNLAARYFGRVIYRKLTASKR 296 TLAVHIWK+NSEGL PDA +A GA+AVL+I VL+FNL+AR+FG +Y+K+T+ Sbjct: 241 TLAVHIWKVNSEGLTPDARDVADGAAAVLIIAVLLFNLSARWFGNWVYKKMTSGSN 296 >B3WCN7.1 B3WCN7_LACCB Phosphate ABC transporter, membrane-spanning subunit Length = 294 Score = 303 bits (775), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 155/294 (52%), Positives = 191/294 (64%) Query: 1 LKPRTADKIATXXXXXXXXXXXXXXXXXXXXXXXXXXNHISWDFLTSAPQKIRAGGGVGP 60 + P+ ADKIAT HISW FLTS Q AGGG+G Sbjct: 1 MNPKVADKIATTVLYIVSGIIIIILASLLGYILFQGLPHISWHFLTSPAQSFEAGGGIGI 60 Query: 61 QLFNSIYXXXXXXXXXXXXXXXAGIFMAEYARPGKLTNFIRLIVEVLSSFPSXXXXXXXX 120 QLFNS Y AGI+++EYA+ LT+ IR +E+LSS PS Sbjct: 61 QLFNSFYLLLLAMLISTPISLGAGIYLSEYAKKNWLTDVIRTSIEILSSLPSVVVGLFGF 120 Query: 121 XXXXNTFDLGFSLVSGALALTFFNLPLMVRITEQAFRTVPKQQKEAGFALGLSKWKIVTN 180 F GFS++SGALALT FNLPL+ R E + R V +Q+EAG ALGLS+W+ V + Sbjct: 121 LIFVLQFKFGFSILSGALALTVFNLPLLTRNVEDSLRGVEYEQREAGLALGLSRWETVLH 180 Query: 181 VLFPVALPTIITGTILSAGRVFGEAAALMFTAGMSSPRLDFSNWNPSSPTSPLNPFRPAE 240 V+ P ALP IITG IL AGRVFGEAAAL++TAG S+P LDF++WNP++ SPLNPFRPAE Sbjct: 181 VVIPEALPGIITGMILGAGRVFGEAAALIYTAGQSAPALDFTDWNPANIASPLNPFRPAE 240 Query: 241 TLAVHIWKINSEGLAPDAAQIAAGASAVLVITVLIFNLAARYFGRVIYRKLTAS 294 TLAVHIWKINSEG+ PDA ++AGASAVLV+ VL+FNL ARYFG +++K+TAS Sbjct: 241 TLAVHIWKINSEGIQPDAIAVSAGASAVLVLAVLLFNLGARYFGNRLFKKMTAS 294 >Q65HB8.1 Q65HB8_BACLD PstA Length = 295 Score = 302 bits (774), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 149/258 (57%), Positives = 187/258 (72%) Query: 38 NHISWDFLTSAPQKIRAGGGVGPQLFNSIYXXXXXXXXXXXXXXXAGIFMAEYARPGKLT 97 +HIS FLTS + +GGG+ QLFNS Y GI+MAEYA KLT Sbjct: 38 SHISLSFLTSRSSALSSGGGIRDQLFNSFYILVLTMLITVPLGVGGGIYMAEYAPANKLT 97 Query: 98 NFIRLIVEVLSSFPSXXXXXXXXXXXXNTFDLGFSLVSGALALTFFNLPLMVRITEQAFR 157 +FIR+ +EVLSS PS N G+S++ GALALT FNLP+MVR+TE A R Sbjct: 98 DFIRVCIEVLSSLPSIVMGMFGMLMFVNLTGWGYSIIGGALALTIFNLPVMVRVTEDAIR 157 Query: 158 TVPKQQKEAGFALGLSKWKIVTNVLFPVALPTIITGTILSAGRVFGEAAALMFTAGMSSP 217 +VPK+QKEA ALG+SKW + VL P A+P+IITG IL++GRVFGEAAAL+FTAG+++P Sbjct: 158 SVPKEQKEASLALGVSKWHTIKTVLIPGAVPSIITGAILASGRVFGEAAALLFTAGLTTP 217 Query: 218 RLDFSNWNPSSPTSPLNPFRPAETLAVHIWKINSEGLAPDAAQIAAGASAVLVITVLIFN 277 RL+F++WNP S TSPLN FRPAETLAVHIW +N++G+ PDA IA GASAVLV++VLIFN Sbjct: 218 RLNFADWNPFSETSPLNIFRPAETLAVHIWNVNTQGIIPDAEAIANGASAVLVLSVLIFN 277 Query: 278 LAARYFGRVIYRKLTASK 295 LAAR+ G I++KLTA+K Sbjct: 278 LAARWLGSFIHKKLTANK 295 >A0ALI1.1 A0ALI1_LISW6 Complete genome Length = 294 Score = 300 bits (768), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 147/291 (50%), Positives = 190/291 (65%) Query: 4 RTADKIATXXXXXXXXXXXXXXXXXXXXXXXXXXNHISWDFLTSAPQKIRAGGGVGPQLF 63 +T DKIAT +SW FLT+ PQ +AGGG+GP+++ Sbjct: 4 KTKDKIATGVFYAIAVFIVIILAGLLGYILVKGVPQLSWKFLTTPPQSFQAGGGIGPEIW 63 Query: 64 NSIYXXXXXXXXXXXXXXXAGIFMAEYARPGKLTNFIRLIVEVLSSFPSXXXXXXXXXXX 123 NS Y AGI+MAEYAR +T+ IR +EVLSS PS Sbjct: 64 NSFYMLVITMIISVPISLGAGIYMAEYARKNWITDLIRTTIEVLSSLPSIVVGLFGFLVF 123 Query: 124 XNTFDLGFSLVSGALALTFFNLPLMVRITEQAFRTVPKQQKEAGFALGLSKWKIVTNVLF 183 FS++SGAL LT FNLPL++R+ E+A +P Q+EAG ALGLS+W+ +T VL Sbjct: 124 VIQMGWSFSVISGALTLTIFNLPLLIRVVEEALNAIPNTQREAGLALGLSRWETITRVLV 183 Query: 184 PVALPTIITGTILSAGRVFGEAAALMFTAGMSSPRLDFSNWNPSSPTSPLNPFRPAETLA 243 P ALP IITG IL+AGRVFGEAAAL+FTAG S+P LDF++WNP +PTSPLN FRPAETLA Sbjct: 184 PAALPAIITGVILAAGRVFGEAAALIFTAGQSTPILDFTDWNPVNPTSPLNIFRPAETLA 243 Query: 244 VHIWKINSEGLAPDAAQIAAGASAVLVITVLIFNLAARYFGRVIYRKLTAS 294 VHIWKIN EG+ PDA ++ GASAVL+++VL+FN+ AR G+V+Y+++T+S Sbjct: 244 VHIWKINGEGIMPDAQAVSDGASAVLILSVLLFNILARLLGKVVYKRMTSS 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,389,276,121 Number of extensions: 48114553 Number of successful extensions: 148252 Number of sequences better than 1.0e-05: 3208 Number of HSP's gapped: 146703 Number of HSP's successfully gapped: 3219 Length of query: 298 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 163 Effective length of database: 1,788,859,178 Effective search space: 291584046014 Effective search space used: 291584046014 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)