BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0054.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D894.1 B1D894_9BACL Phosphate binding protein 337 9e-91 A5D1L6.1 A5D1L6_PELTS ABC-type phosphate transport system, perme... 296 2e-78 C1TFA3.1 C1TFA3_9FIRM Phosphate ABC transporter substrate-bindin... 287 9e-76 Q8R9H9.1 Q8R9H9_THETN ABC-type phosphate transport system, perip... 286 2e-75 B7R7V5.1 B7R7V5_9THEO Bacterial extracellular solute-binding pro... 286 2e-75 >B1D894.1 B1D894_9BACL Phosphate binding protein Length = 311 Score = 337 bits (864), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 173/238 (72%), Positives = 200/238 (84%), Gaps = 3/238 (1%) Query: 47 LSGSILASGSTALQPLVEQVAEKFMETNKDVDIQVQGGGSGTGLTQVAEKQVDIGNSDVF 106 +SGSILA+GSTALQPLV+QV+++FM+ N V IQVQGGGSGTGLTQV+E Q IGNSDVF Sbjct: 48 VSGSILAAGSTALQPLVDQVSKEFMDKNSGVSIQVQGGGSGTGLTQVSEGQAQIGNSDVF 107 Query: 107 XXXXXXXXXXXXXXXXVDHQVAVVAIAAVSHPDAGVDSLTKQQLVDIFTGKVTNWKEVGG 166 VDHQVAVVA+AAV++ D GVD+LTKQQLVDIFTGKVTNWK+VGG Sbjct: 108 AEEKLDADKAAEL---VDHQVAVVAMAAVTNKDVGVDTLTKQQLVDIFTGKVTNWKDVGG 164 Query: 167 KDQKIQIINRPSSSGTRATFEGFALGTKTEDLQGSIQEDSSGTVKKMIGETPGAIGYLAL 226 KD KIQI+NRPSSSGTRATFE FALGTKTEDLQGSIQED+SGTVKK++ ETPGAIGYLAL Sbjct: 165 KDAKIQIVNRPSSSGTRATFEKFALGTKTEDLQGSIQEDASGTVKKLVSETPGAIGYLAL 224 Query: 227 SYLDDSVKTLKYDGVEPSVDNVVSGKYPVWAYEHMYTNGEPNEIVKAFLDYIMTPEVQ 284 SYLDDS+ +KY+GVE +VDNV +G YPVWAYEHMYT GEP+ KAFLDY+++ +VQ Sbjct: 225 SYLDDSIAAVKYEGVEANVDNVANGTYPVWAYEHMYTKGEPDAATKAFLDYMVSEDVQ 282 >A5D1L6.1 A5D1L6_PELTS ABC-type phosphate transport system, permease component Length = 297 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 147/238 (61%), Positives = 181/238 (76%), Gaps = 6/238 (2%) Query: 47 LSGSILASGSTALQPLVEQVAEKFMETNKDVDIQVQGGGSGTGLTQVAEKQVDIGNSDVF 106 LSG+I A GSTA+QPLVEQ A +FM + +V I VQGGGSGTGLTQVA+ DIGNSD+F Sbjct: 38 LSGNITAVGSTAMQPLVEQAANQFMAKHPNVKIVVQGGGSGTGLTQVAQGAADIGNSDIF 97 Query: 107 XXXXXXXXXXXXXXXXVDHQVAVVAIAAVSHPDAGVDSLTKQQLVDIFTGKVTNWKEVGG 166 VDHQ+ VV +AAV +P +D+LT+QQLVDIFTGK+TNWK+VGG Sbjct: 98 AEEKDGIDASQL----VDHQICVVGMAAVVNPAVNIDNLTRQQLVDIFTGKITNWKDVGG 153 Query: 167 KDQKIQIINRPSSSGTRATFEGFALGTKTEDLQGSIQEDSSGTVKKMIGETPGAIGYLAL 226 DQK+ ++NRP +SGTRATF+ +ALG E I+EDSSGTV+K++ ETPGAIGYLAL Sbjct: 154 PDQKVVLVNRPKASGTRATFKKYALGGAEE--AAGIEEDSSGTVRKIVKETPGAIGYLAL 211 Query: 227 SYLDDSVKTLKYDGVEPSVDNVVSGKYPVWAYEHMYTNGEPNEIVKAFLDYIMTPEVQ 284 SYLD SVK +K DGVEPS +N+ SGKYPVWAY+H YT G+P+ ++AFL+YIMT EVQ Sbjct: 212 SYLDGSVKAIKLDGVEPSKENITSGKYPVWAYQHSYTKGKPSGALEAFLNYIMTDEVQ 269 >C1TFA3.1 C1TFA3_9FIRM Phosphate ABC transporter substrate-binding protein, PhoT family Length = 323 Score = 287 bits (735), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 144/239 (60%), Positives = 177/239 (74%), Gaps = 5/239 (2%) Query: 46 DLSGSILASGSTALQPLVEQVAEKFMETNKDVDIQVQGGGSGTGLTQVAEKQVDIGNSDV 105 ++SG+I A GSTALQPLVEQ A ++M N I VQGGGSGTGLTQVA DIGNSD+ Sbjct: 62 EVSGNITAVGSTALQPLVEQAASQYMAKNSKTQIVVQGGGSGTGLTQVASGAADIGNSDI 121 Query: 106 FXXXXXXXXXXXXXXXXVDHQVAVVAIAAVSHPDAGVDSLTKQQLVDIFTGKVTNWKEVG 165 F VD++V VV + V +P V++LTKQQLVDIFTGKVT+WK+VG Sbjct: 122 FAEQKDGIDASQL----VDNKVCVVGMTTVVNPGVTVNNLTKQQLVDIFTGKVTDWKDVG 177 Query: 166 GKDQKIQIINRPSSSGTRATFEGFALGTKTEDLQGSIQEDSSGTVKKMIGETPGAIGYLA 225 G +QKI +I+RP SGTRATF+ FALG E G +++DSSGTV+KM+GETPGAI YLA Sbjct: 178 GSEQKIVVIHRPKGSGTRATFDSFALGGTDETAPG-MEQDSSGTVRKMVGETPGAISYLA 236 Query: 226 LSYLDDSVKTLKYDGVEPSVDNVVSGKYPVWAYEHMYTNGEPNEIVKAFLDYIMTPEVQ 284 +SY+D+S+K LK D VEPSV+N+ +GKYPVWAYEHMYT G P + KAFLDY++T EVQ Sbjct: 237 ISYVDNSIKALKLDSVEPSVENIATGKYPVWAYEHMYTKGAPTGVKKAFLDYMLTDEVQ 295 >Q8R9H9.1 Q8R9H9_THETN ABC-type phosphate transport system, periplasmic component Length = 291 Score = 286 bits (732), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 143/239 (59%), Positives = 175/239 (73%), Gaps = 3/239 (1%) Query: 46 DLSGSILASGSTALQPLVEQVAEKFMETNKDVDIQVQGGGSGTGLTQVAEKQVDIGNSDV 105 +LSG+I +GSTALQPLVEQ A+ FME +V I VQGGGSGTGLTQV + +DIGNSD+ Sbjct: 43 ELSGNITIAGSTALQPLVEQAAKLFMEKYPNVSITVQGGGSGTGLTQVVQGAIDIGNSDI 102 Query: 106 FXXXXXXXXXXXXXXXXVDHQVAVVAIAAVSHPDAGVDSLTKQQLVDIFTGKVTNWKEVG 165 F VDHQVA V A V + D VD LT+QQL+DIFTGK+TNWKEVG Sbjct: 103 FAEEKLDPNIAKQL---VDHQVAKVGFAVVVNKDVTVDDLTQQQLIDIFTGKITNWKEVG 159 Query: 166 GKDQKIQIINRPSSSGTRATFEGFALGTKTEDLQGSIQEDSSGTVKKMIGETPGAIGYLA 225 G D KI IINRP+SSGTRATF+ L + E ++ EDSSG V+K + +T GAI YLA Sbjct: 160 GPDMKIVIINRPTSSGTRATFKKVVLKGQEEAQGIALTEDSSGAVRKAVADTKGAISYLA 219 Query: 226 LSYLDDSVKTLKYDGVEPSVDNVVSGKYPVWAYEHMYTNGEPNEIVKAFLDYIMTPEVQ 284 LSY+D+SVK LKY+GVEP+V N++ GKYP+W+YEHMYT GEP VKAFLD++++ EVQ Sbjct: 220 LSYVDNSVKALKYEGVEPTVQNIIDGKYPIWSYEHMYTKGEPKGAVKAFLDFMLSDEVQ 278 >B7R7V5.1 B7R7V5_9THEO Bacterial extracellular solute-binding protein, putative Length = 291 Score = 286 bits (732), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 143/239 (59%), Positives = 175/239 (73%), Gaps = 3/239 (1%) Query: 46 DLSGSILASGSTALQPLVEQVAEKFMETNKDVDIQVQGGGSGTGLTQVAEKQVDIGNSDV 105 +LSG+I +GSTALQPLVEQ A+ FME +V I VQGGGSGTGLTQV + +DIGNSD+ Sbjct: 43 ELSGNITIAGSTALQPLVEQAAKLFMEKYPNVSITVQGGGSGTGLTQVVQGAIDIGNSDI 102 Query: 106 FXXXXXXXXXXXXXXXXVDHQVAVVAIAAVSHPDAGVDSLTKQQLVDIFTGKVTNWKEVG 165 F VDHQVA V A V + D VD LT+QQL+DIFTGK+TNWKEVG Sbjct: 103 FAEEKLDPNIAKQL---VDHQVAKVGFAVVVNKDVTVDDLTQQQLIDIFTGKITNWKEVG 159 Query: 166 GKDQKIQIINRPSSSGTRATFEGFALGTKTEDLQGSIQEDSSGTVKKMIGETPGAIGYLA 225 G D KI IINRP+SSGTRATF+ L + E ++ EDSSG V+K + +T GAI YLA Sbjct: 160 GPDMKIVIINRPTSSGTRATFKKVVLKGQEEAQGIALTEDSSGAVRKAVADTKGAISYLA 219 Query: 226 LSYLDDSVKTLKYDGVEPSVDNVVSGKYPVWAYEHMYTNGEPNEIVKAFLDYIMTPEVQ 284 LSY+D+SVK LKY+GVEP+V N++ GKYP+W+YEHMYT GEP VKAFLD++++ EVQ Sbjct: 220 LSYVDNSVKALKYEGVEPTVQNIIDGKYPIWSYEHMYTKGEPKGAVKAFLDFMLSDEVQ 278 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,557,061,282 Number of extensions: 61086162 Number of successful extensions: 121694 Number of sequences better than 1.0e-05: 1344 Number of HSP's gapped: 120057 Number of HSP's successfully gapped: 1377 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)