BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0049.p (245 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GLA2.1 B7GLA2_ANOFW Organic radical activating enzyme 303 1e-80 C0ZJI7.1 C0ZJI7_BREBN Putative queuosine biosynthesis protein 301 5e-80 C5D7Y4.1 C5D7Y4_9BACI 7-cyano-7-deazaguanosine (PreQ0) biosynthe... 299 2e-79 B1DIS4.1 B1DIS4_9BACL 7-cyano-7-deazaguanosine (PreQ0) biosynthe... 295 2e-78 B5UI69.1 B5UI69_BACCE 7-cyano-7-deazaguanosine (PreQ0) biosynthe... 295 3e-78 >B7GLA2.1 B7GLA2_ANOFW Organic radical activating enzyme Length = 240 Score = 303 bits (776), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 144/241 (59%), Positives = 183/241 (75%), Gaps = 4/241 (1%) Query: 6 VSKLPVIEIFGPTIQGEGAVIGVKTMFVRTYGCDYRCSWCDSAFTWDGSAKDKVRMLAPE 65 + +PVIE+FGPTIQGEG VIG KTMFVRT GCDYRC WCDS+FTWDGSAK++V+ + E Sbjct: 1 MEHIPVIEMFGPTIQGEGMVIGQKTMFVRTAGCDYRCRWCDSSFTWDGSAKNEVKQMTAE 60 Query: 66 EIMDELAALAGGNFDCVTLSGGNPALIGEGMGRLIQLLHERGIQAAVETQGSRWQNWFYA 125 +I +L L G F+ VT+SGGNPAL+ +G+ LI LL E I+ +ETQGS WQ+WFY Sbjct: 61 DIWQQLKQLGGDRFNHVTISGGNPALL-KGLRALISLLKEHNIRIGLETQGSVWQDWFYD 119 Query: 126 VDVLTVSPKPPSSGMETDWNMLDGIMDKMKAHGRATHSLKVVVFNDEDYQYAKRVHSRYP 185 +D LT+SPKPPSS M+T++ MLD IM+++ AH R SLKVVVF+DED+ YAK VH RYP Sbjct: 120 IDDLTISPKPPSSNMKTNFTMLDTIMERLTAH-RGHVSLKVVVFDDEDFAYAKHVHQRYP 178 Query: 186 GVPFFLQPGNEDVTEEGDISVR--LLARLEWLFNLVIADPEMNKARVLPQLHALIWHNKR 243 VPF++Q GN+ + E D+++R LL +LEWL V+ EMN RVLPQLHAL+W N+R Sbjct: 179 TVPFYVQVGNDHIHEADDVTLRLQLLQKLEWLVEQVVQSNEMNDVRVLPQLHALLWGNRR 238 Query: 244 G 244 G Sbjct: 239 G 239 >C0ZJI7.1 C0ZJI7_BREBN Putative queuosine biosynthesis protein Length = 239 Score = 301 bits (770), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 142/238 (59%), Positives = 179/238 (75%), Gaps = 3/238 (1%) Query: 9 LPVIEIFGPTIQGEGAVIGVKTMFVRTYGCDYRCSWCDSAFTWDGSAKDKVRMLAPEEIM 68 +PV+EIFGPTIQGEG VIG KTMFVRT GCDYRC+WCDSAFTWDGSA+D++R ++PE + Sbjct: 2 IPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYRCNWCDSAFTWDGSARDEIRQMSPEAVW 61 Query: 69 DELAALAGGNFDCVTLSGGNPALIGEGMGRLIQLLHERGIQAAVETQGSRWQNWFYAVDV 128 +EL L G F VT+SGGNPAL+ G+G LI LL E GI+ AVETQGS+WQ W +D Sbjct: 62 EELTRLGGDRFSHVTISGGNPALLA-GIGDLISLLKEHGIRTAVETQGSKWQAWLPLIDD 120 Query: 129 LTVSPKPPSSGMETDWNMLDGIMDKMKAHGRATHSLKVVVFNDEDYQYAKRVHSRYPGVP 188 +T+SPKPPSSGM+TD+ LD I+ ++ SLKVVVF+D D+ YA+ +H R+PGVP Sbjct: 121 ITLSPKPPSSGMQTDFQALDRIVHELLDQKHPGLSLKVVVFDDADFAYARTIHQRFPGVP 180 Query: 189 FFLQPGNEDVTEEGDISVR--LLARLEWLFNLVIADPEMNKARVLPQLHALIWHNKRG 244 F+LQPGN D+T+ S+R LL EWL + + +MN A+VLPQLHAL+W NKRG Sbjct: 181 FYLQPGNSDLTDADTPSLRDKLLESFEWLIDQAMETADMNDAKVLPQLHALVWGNKRG 238 >C5D7Y4.1 C5D7Y4_9BACI 7-cyano-7-deazaguanosine (PreQ0) biosynthesis protein QueE Length = 245 Score = 299 bits (765), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 148/245 (60%), Positives = 184/245 (75%), Gaps = 4/245 (1%) Query: 3 VVTVSKLPVIEIFGPTIQGEGAVIGVKTMFVRTYGCDYRCSWCDSAFTWDGSAKDKVRML 62 ++ K+PV+EIFGPTIQGEG VIG KTMFVRT GCDYRC WCDSAFTWDGSAK+++R + Sbjct: 1 MIMAKKIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIRQM 60 Query: 63 APEEIMDELAALAGGNFDCVTLSGGNPALIGEGMGRLIQLLHERGIQAAVETQGSRWQNW 122 E+I + L L G F VT+SGGNP LI + + L+ LL +GI+ AVETQGSRWQ+W Sbjct: 61 TAEQIWERLYELGGDRFSHVTISGGNPVLI-QALEELVVLLKNKGIRIAVETQGSRWQDW 119 Query: 123 FYAVDVLTVSPKPPSSGMETDWNMLDGIMDKMKAHGRATH-SLKVVVFNDEDYQYAKRVH 181 Y +D +T+SPKPPSSGM+TD+ MLD I+ K+ GRA+H SLKVVVF++ D+ YAK+VH Sbjct: 120 LYHIDDITISPKPPSSGMDTDFAMLDHIVGKLAGAGRASHMSLKVVVFDEIDFDYAKKVH 179 Query: 182 SRYPGVPFFLQPGNEDVTEEGDISVR--LLARLEWLFNLVIADPEMNKARVLPQLHALIW 239 RYP +PF+LQ GN D+ E D ++R LLARLEWL V E+N RVLPQLH L+W Sbjct: 180 RRYPDIPFYLQVGNADLAEADDAALRTQLLARLEWLVEKVAQSNELNDVRVLPQLHTLLW 239 Query: 240 HNKRG 244 NKRG Sbjct: 240 GNKRG 244 >B1DIS4.1 B1DIS4_9BACL 7-cyano-7-deazaguanosine (PreQ0) biosynthesis protein QueE Length = 242 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 141/239 (58%), Positives = 176/239 (73%), Gaps = 4/239 (1%) Query: 8 KLPVIEIFGPTIQGEGAVIGVKTMFVRTYGCDYRCSWCDSAFTWDGSAKDKVRMLAPEEI 67 ++PV+EIFGPT+QGEG VIG KTMFVRT GCDY CSWCDSAFTWDG+ KD RML EI Sbjct: 5 RIPVMEIFGPTVQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGTGKDDTRMLTASEI 64 Query: 68 MDELAALAGGNFDCVTLSGGNPALIGEGMGRLIQLLHERGIQAAVETQGSRWQNWFYAVD 127 + EL L G F VTLSGGNPAL+ + L+ LH G++ AVETQGSRWQ+W A+D Sbjct: 65 VGELRELGGNTFSHVTLSGGNPALL-KNADELVDALHRDGVRVAVETQGSRWQDWLLAID 123 Query: 128 VLTVSPKPPSSGMETDWNMLDGIMDKMKAHGRATHSLKVVVFNDEDYQYAKRVHSRYPGV 187 LT+SPKPPSSGM T+W +LD I+ ++ A G SLKVVVF+++D YA +VH+RYPGV Sbjct: 124 ELTLSPKPPSSGMTTNWEVLDEIVARL-AGGSNPFSLKVVVFDEQDLAYAAKVHNRYPGV 182 Query: 188 PFFLQPGNEDVTE--EGDISVRLLARLEWLFNLVIADPEMNKARVLPQLHALIWHNKRG 244 FF+Q GN+D+ E G ++ +L+ R EWL + V+ +M RVLPQLHAL+W NKRG Sbjct: 183 TFFVQTGNDDLIEGDNGKLAAKLIERYEWLIDQVMKREDMTNVRVLPQLHALVWGNKRG 241 >B5UI69.1 B5UI69_BACCE 7-cyano-7-deazaguanosine (PreQ0) biosynthesis protein QueE Length = 238 Score = 295 bits (755), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 138/241 (57%), Positives = 181/241 (75%), Gaps = 6/241 (2%) Query: 6 VSKLPVIEIFGPTIQGEGAVIGVKTMFVRTYGCDYRCSWCDSAFTWDGSAKDKVRMLAPE 65 +SK+PV+EIFGPTIQGEG V+G KTMF+RT GCDY C+WCDSAFTWDGSAKD++R + PE Sbjct: 1 MSKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPE 60 Query: 66 EIMDELAALAGGNFDCVTLSGGNPALIGEGMGRLIQLLHERGIQAAVETQGSRWQNWFYA 125 +I DEL A+ G NF VT+SGGNPAL+ + + L+ +L E G++ A+ETQGS+WQ+W Sbjct: 61 DIWDELVAIGGENFSHVTISGGNPALL-KNIEFLLSILKENGMRTAIETQGSKWQDWLLQ 119 Query: 126 VDVLTVSPKPPSSGMETDWNMLDGIMDKMKAHGRATHSLKVVVFNDEDYQYAKRVHSRYP 185 +D +T+SPKPPSS M+TD++ LD I+ K+ SLKVVVF+D D++YA ++H RYP Sbjct: 120 IDEVTISPKPPSSTMKTDFHKLDAIIQKLAGKDI---SLKVVVFDDHDFEYAVKMHERYP 176 Query: 186 GVPFFLQPGNEDVTEEGDISV--RLLARLEWLFNLVIADPEMNKARVLPQLHALIWHNKR 243 VPFFLQ GN+D D + +LL + EWL + + EMN A+VLPQLHAL+W NKR Sbjct: 177 KVPFFLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKR 236 Query: 244 G 244 G Sbjct: 237 G 237 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,613,199,108 Number of extensions: 67938304 Number of successful extensions: 152605 Number of sequences better than 1.0e-05: 737 Number of HSP's gapped: 152028 Number of HSP's successfully gapped: 737 Length of query: 245 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 113 Effective length of database: 1,817,122,223 Effective search space: 205334811199 Effective search space used: 205334811199 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)