BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0024.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3CTM0.1 C3CTM0_BACTU Short chain dehydrogenase 288 4e-76 C0V1C6.1 C0V1C6_9BACT Putative uncharacterized protein 209 3e-52 B7KMT1.1 B7KMT1_CYAP7 Short-chain dehydrogenase/reductase SDR 201 5e-50 C0Z6B7.1 C0Z6B7_BREBN Glucose 1-dehydrogenase EC=1.1.1.47 199 2e-49 C4WJN4.1 C4WJN4_9RHIZ Short-chain dehydrogenase/reductase 193 1e-47 >C3CTM0.1 C3CTM0_BACTU Short chain dehydrogenase Length = 254 Score = 288 bits (737), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 148/251 (58%), Positives = 187/251 (74%), Gaps = 1/251 (0%) Query: 8 MRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADI-DAEGNHSAQQLQRAGLAVTFCR 66 M+L K AIVTG ARGIG + R+F +EGA VV+AD+ + EGN A +L+R G V F Sbjct: 1 MKLENKVAIVTGGARGIGGAIVRKFIDEGAKVVIADVLEKEGNALAVELRRQGHEVFFVY 60 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 TDV++ D+V ++VD + G IDIL NNA VNIPGS++ELSEE W++T VNVKS FL+ Sbjct: 61 TDVSNADAVKRVVDYTFDKFGRIDILSNNAGVNIPGSVIELSEENWEKTMNVNVKSYFLM 120 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 ++ V+P MQ++G GA+VNM SANS AE RLSAYV+SKGAI ML+K MA+DFA D IRVN Sbjct: 121 AKYVLPYMQKAGGGAVVNMASANSEFAEQRLSAYVTSKGAIKMLTKQMAIDFAKDNIRVN 180 Query: 187 CICPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAIT 246 +CPGWVDT FND+HA LFGGREE+LK + I +GR IQP EIAN FL SDE+S + Sbjct: 181 GVCPGWVDTTFNDAHAELFGGREEVLKELPAIQKLGRAIQPKEIANIVAFLASDESSCMV 240 Query: 247 GAGILVDGGYT 257 G+ ++ DGG T Sbjct: 241 GSTVMADGGIT 251 >C0V1C6.1 C0V1C6_9BACT Putative uncharacterized protein Length = 254 Score = 209 bits (531), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 110/249 (44%), Positives = 147/249 (59%), Gaps = 1/249 (0%) Query: 8 MRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAEGNHS-AQQLQRAGLAVTFCR 66 MRL GK A++TGAA GIG A FAE GA +V+ D+DAEG QL+ G V Sbjct: 1 MRLDGKVAVITGAASGIGRGTAEVFAEHGARLVLVDLDAEGLAGLVGQLKAGGAEVVGIS 60 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 DV+ + V+ V A+ +G ID++ NNA V G+ EL +++WDR VN++ +L Sbjct: 61 GDVSHKATAVEAVGAALRQYGRIDVVFNNAGVMYSGAFHELGDDLWDRVLDVNLRGTYLF 120 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 R IP M G G+I+NM S + + EP AY +SK I+ L+KA+A+ +A GIR N Sbjct: 121 CREAIPHMLEQGRGSIINMSSVMATLTEPGYEAYTTSKAGIIGLTKAIAVSYAERGIRCN 180 Query: 187 CICPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAIT 246 C+CPGWVDT N A GG + + I P GR + E+AN LFL SDE+S +T Sbjct: 181 CVCPGWVDTPLNRRLAEELGGMDRLNALIKQQQPNGRMLSTREVANAVLFLASDESSGVT 240 Query: 247 GAGILVDGG 255 GA I VDGG Sbjct: 241 GAAIYVDGG 249 >B7KMT1.1 B7KMT1_CYAP7 Short-chain dehydrogenase/reductase SDR Length = 254 Score = 201 bits (511), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 106/253 (41%), Positives = 154/253 (60%), Gaps = 4/253 (1%) Query: 8 MRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAEGNHSAQQLQRAGLA-VTFCR 66 MRL K A++TG GIG A+ FA +GA+V +ADID +G + R L F Sbjct: 1 MRLANKIALITGGGSGIGAATAKLFASQGASVALADIDEQGGQAVVTEIRQSLGEALFHL 60 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 D++ V Q ++T + GG+DILVNNAA + G++ E+S E WD+ VNVK Sbjct: 61 CDISQEQQVKQWIETVAQTWGGVDILVNNAATFVFGNVEEVSGEDWDKILSVNVKGYAFC 120 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 ++ P+M++ G G+IVN+GS +S +A+ Y +SKGAIL +++ +A D A D IRVN Sbjct: 121 AKYAAPLMRQRGGGSIVNLGSISSVIAQKSFVPYNTSKGAILQMTRCLAYDLAPDNIRVN 180 Query: 187 CICPGWVDTA--FNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASA 244 C+CPG +DT + D+ + +EE ++ H +GR QP+E+A+ LFL S EAS Sbjct: 181 CVCPGTIDTPAIWRDAGSKNL-TQEEFIEQAAQQHLLGRIGQPIEVAHAILFLASSEASF 239 Query: 245 ITGAGILVDGGYT 257 ITG ++VDGGYT Sbjct: 240 ITGTSLMVDGGYT 252 >C0Z6B7.1 C0Z6B7_BREBN Glucose 1-dehydrogenase EC=1.1.1.47 Length = 248 Score = 199 bits (506), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 110/253 (43%), Positives = 152/253 (60%), Gaps = 13/253 (5%) Query: 9 RLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAEGNHSAQQLQRAGLAVTFCRTD 68 RL GK AIVTG A GIG R FA+EGA VV+AD GN A++L AG F +TD Sbjct: 3 RLEGKVAIVTGGASGIGETTVRLFAKEGAKVVIADFSPRGNELAEELNLAGFDALFVKTD 62 Query: 69 VASNDSVVQLVDTAMELHGGIDILVNNAAV--NIPGSILELSEEIWDRTYQVNVKSMFLL 126 V D V +V + +G +DIL NA + + PG +L L + W RT +N+ +FL Sbjct: 63 VTKEDEVKNMVSATVVKYGKVDILFANAGIAKDAPGHLLSLDD--WQRTIDINLTGVFLC 120 Query: 127 SRAVIPIMQRSGS-GAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRV 185 + VI M G+ GAIVN GS +S+ + ++AY S+KG + +L++ + L +A GIRV Sbjct: 121 DKYVIEQMLAQGTGGAIVNCGSIHSHAGKAGVTAYSSAKGGVKLLTQTLGLTYAKQGIRV 180 Query: 186 NCICPGWVDTAFNDSHAALFGGREEIL-KSIHDIHPIGRTIQPVEIANTALFLVSDEASA 244 N +CPG++DT L GR E L + + +HP+GR +P E+A LFL SD+AS Sbjct: 181 NAVCPGYIDT-------PLIAGRNEALNEHLIGLHPMGRLGKPEEVAKAVLFLASDDASF 233 Query: 245 ITGAGILVDGGYT 257 +TG +LVDGGYT Sbjct: 234 VTGTSLLVDGGYT 246 >C4WJN4.1 C4WJN4_9RHIZ Short-chain dehydrogenase/reductase Length = 248 Score = 193 bits (490), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 105/251 (41%), Positives = 148/251 (58%), Gaps = 6/251 (2%) Query: 7 AMRLTGKKAIVTGAARGIGYEVARRFAEEGAAVVMADIDAEGNHSAQQLQRAGLAVTFCR 66 AMR GK AIVTG A GIG AR F EGA VV+AD G A +L F + Sbjct: 2 AMRFDGKVAIVTGGASGIGEATARAFIREGANVVIADYSDHGQQLANELAGGSERALFVK 61 Query: 67 TDVASNDSVVQLVDTAMELHGGIDILVNNAAVNIPGSILELSEEIWDRTYQVNVKSMFLL 126 TDVA +V L+ +E +G +DI+ NA + G I EL E W +T +N+ ++L Sbjct: 62 TDVADTKAVQALIAKTVETYGRLDIMFANAGIAADGPIDELDETAWQKTIDINLTGVYLC 121 Query: 127 SRAVIPIMQRSGSGAIVNMGSANSYVAEPRLSAYVSSKGAILMLSKAMALDFAADGIRVN 186 + I M+ G G IVN GS +S+V + ++AY ++KG + +L++ +A+D+ A IRVN Sbjct: 122 DKYAIDQMRSQGGGVIVNCGSIHSHVGKSGVTAYAAAKGGVKLLTQTLAIDYGAQNIRVN 181 Query: 187 CICPGWVDTAFNDSHAALFGGREEILKSIHDIHPIGRTIQPVEIANTALFLVSDEASAIT 246 +CPG++DT L E+ +++ +HPIGR + E+A+ LFL SDEAS +T Sbjct: 182 AVCPGYIDTPL------LKDIPEDKKEALVALHPIGRLGRAEEVASVVLFLASDEASFVT 235 Query: 247 GAGILVDGGYT 257 GA ILVDGGYT Sbjct: 236 GASILVDGGYT 246 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,383,258,742 Number of extensions: 50759957 Number of successful extensions: 301212 Number of sequences better than 1.0e-05: 34010 Number of HSP's gapped: 239710 Number of HSP's successfully gapped: 34103 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)