BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0021.p (442 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9LA76.1 Q9LA76_STRVF Valanimycin resistance 161 1e-37 C3JLA6.1 C3JLA6_RHOER Valanimycin resistance 136 5e-30 C0ZVL5.1 C0ZVL5_RHOE4 Putative drug resistance efflux protein 133 4e-29 A8KXX9.1 A8KXX9_FRASN Major facilitator superfamily MFS_1 127 2e-27 Q1QSI2.1 Q1QSI2_CHRSD Major facilitator superfamily MFS_1 126 3e-27 >Q9LA76.1 Q9LA76_STRVF Valanimycin resistance Length = 402 Score = 161 bits (408), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 106/335 (31%), Positives = 165/335 (49%), Gaps = 3/335 (0%) Query: 87 YWAVAYGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDR 146 +WA AY L + G NL PLY Y ++ SP IPS+++ G +SD Sbjct: 22 FWATAYTLLFLLTGTNLPTPLYRGYEHKFGFSPLVVTLIFAVYVASLIPSLLIAGPLSDS 81 Query: 147 IGPVRILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANS 206 IG ++LL + GSL F A+ + L +AR+LQGLAVG +G +A++EL + Sbjct: 82 IGRRKVLLPAVLLAAVGSLVFALADNVGWLFVARLLQGLAVGAASGALTAALSELEPSGD 141 Query: 207 KRQAAYLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLVRIRSE 266 +R+AA ++ + G GP+++G+LAEY P P +PF+V ++L+VPA I + + S Sbjct: 142 RRRAALVSTVASVGGLGAGPLIAGLLAEYAPSPYVLPFVVEIVLLVPAAIAMATLP-TSR 200 Query: 267 PAGTASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXX 326 PA P +PA +RG F ++ SFL + V+ LF+S+VPTY+ + Sbjct: 201 PATKWRPRRPSIPATMRGVFVSSGTASFLAFSVIGLFLSLVPTYVTKLSGSSNLALAGGA 260 Query: 327 XXXXXXXXXXXQFTL-GKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGM 385 Q G+ PL +T SL +L A+ ++ G+ Sbjct: 261 VALMLACSVIAQIAASGRQPLAIQITGLVLLAVGLVLLAVAGGV-GSLALLLAATVIGGV 319 Query: 386 GHGPLYAGSLAYVNAIAPSEARGDTVSFFYTGTYI 420 G G + G + +N +AP E D +S FY Y+ Sbjct: 320 GQGLAFLGGITEINRVAPKERHADVLSSFYVVIYL 354 >C3JLA6.1 C3JLA6_RHOER Valanimycin resistance Length = 389 Score = 136 bits (342), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 102/336 (30%), Positives = 163/336 (48%), Gaps = 4/336 (1%) Query: 92 YGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVR 151 Y L V +LG N+ +Y +YRT++ +P +P+++ FG +SD +G Sbjct: 3 YVLTVILLGANMPTSVYGLYRTEFGFTPTIQTLIYGVYVAGLVPALLFFGPLSDALGRRT 62 Query: 152 ILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAA 211 +LL +A ++ G+L FA+ L + R+ QG+AVG + +A+ E R+AA Sbjct: 63 VLLTALALSVVGTLVLAFADATVWLFVGRIAQGIAVGAASAAGSAALVEQEPKFDHRRAA 122 Query: 212 YLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLV-RIRSEPAGT 270 ++ + G ALGP++SG +A+Y P P R+P+L+ L+ +PA+I LLL+ RI Sbjct: 123 LVSTVTGALGGALGPLVSGAIAQYLPQPLRLPYLIFLVAFIPALIVLLLLPRIGDVTRPK 182 Query: 271 ASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXXXXXX 330 P VPA IR F+ +SL L WG V LF SVVP+++ ++ Sbjct: 183 RFFQMPHVPAAIRETFWLSSLAVALSWGAVGLFQSVVPSWMTSLLNIDNLLLGGGAAALV 242 Query: 331 XXXXXXXQFTLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGMGHGPL 390 Q ++ +T SL +L ++VG GHG Sbjct: 243 MICSVVAQLGGSRMDPRTSVTAGLGILALGMIGLLVVDLVPSLALLGVITVVVGAGHGLA 302 Query: 391 YAGSLAYVNAIAPSEAR---GDTVSFFYTGTYIGVA 423 +AG + VNA A + AR G ++ F+T +Y G+A Sbjct: 303 FAGGMKRVNAAARTHARESHGGVLAAFFTVSYAGLA 338 >C0ZVL5.1 C0ZVL5_RHOE4 Putative drug resistance efflux protein Length = 389 Score = 133 bits (334), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 101/338 (29%), Positives = 162/338 (47%), Gaps = 4/338 (1%) Query: 90 VAYGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGP 149 + Y L V +LG N+ +Y +YRT++ +P +P+++ FG +SD +G Sbjct: 1 MGYVLTVILLGANMPTSVYGLYRTEFGFTPTIQTLIYGVYVAGLVPALLFFGPLSDALGR 60 Query: 150 VRILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQ 209 +LL +A ++ G+L FA+ L + RV QG+AVG + +A+ E R+ Sbjct: 61 RTVLLTALALSVVGTLVLAFADATVWLFVGRVAQGIAVGAASAAGSAALVEQEPKFDHRR 120 Query: 210 AAYLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLV-RIRSEPA 268 AA ++ + G ALGP++SG +A+Y P P R+P+L+ L+ +PA+I LLL+ RI Sbjct: 121 AALVSTVTGALGGALGPLVSGAIAQYLPQPLRLPYLIFLVAFIPALIVLLLLPRIGDITR 180 Query: 269 GTASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXXXX 328 P VPA IR F+ +SL L WG V LF SVVP+++ ++ Sbjct: 181 PKRFFQMPHVPATIRETFWLSSLAVALSWGAVGLFQSVVPSWMTSLLNIDNLLLGGGAAA 240 Query: 329 XXXXXXXXXQFTLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGMGHG 388 Q ++ +T S +L ++VG GHG Sbjct: 241 LVMICSVIAQLGGSRMDPRTSVTAGLGILALGMIGLLVVDLVPSFALLGVITVVVGAGHG 300 Query: 389 PLYAGSLAYVNA---IAPSEARGDTVSFFYTGTYIGVA 423 +AG + VNA I E+ G ++ F+T +Y G+A Sbjct: 301 LAFAGGMKRVNAAARIHARESHGGVLAAFFTVSYAGLA 338 >A8KXX9.1 A8KXX9_FRASN Major facilitator superfamily MFS_1 Length = 414 Score = 127 bits (319), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 77/227 (33%), Positives = 126/227 (55%), Gaps = 2/227 (0%) Query: 92 YGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVR 151 Y VT+LG L P+Y +YR ++ S I +++LFG++SD++G Sbjct: 8 YAFVVTMLGTTLPTPIYPLYRERFGFSQLMVTVVFATYAVGVIAALLLFGRLSDQVGRRH 67 Query: 152 ILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAA 211 LL G+ A+ + + FL A+ + +LL+ RVL GL+ G+F G A + + +L + + +A Sbjct: 68 ALLPGLLASAASAAVFLLADNVGVLLVGRVLSGLSAGVFTGTATATLLDLAPPHRRGRAT 127 Query: 212 YLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLVRIRSEPAGTA 271 +A + G GP+L+G+L++Y P P R+ F+V L L+VPA +G+ L+ E G A Sbjct: 128 LVATIANMGGLGCGPLLAGLLSQYAPNPLRLTFVVDLALLVPAAVGIWLMPETVEVTGPA 187 Query: 272 SL--HWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVD 316 L PRVP R F +L +F + V+ L+ +V P +LG +D Sbjct: 188 RLRPQRPRVPPSARSVFVGVALAAFAGFAVLGLYTAVAPAFLGAVLD 234 >Q1QSI2.1 Q1QSI2_CHRSD Major facilitator superfamily MFS_1 Length = 397 Score = 126 bits (317), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 96/341 (28%), Positives = 146/341 (42%), Gaps = 2/341 (0%) Query: 85 LSYWAVAYGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVS 144 L++ VA+ VT+LG L PLY Y+T + S I ++++ G+ S Sbjct: 10 LAFAGVAFAFLVTMLGTTLPTPLYPGYQTAFGFSQLIITVIFAAYAVGVIAALLVAGRWS 69 Query: 145 DRIGPVRILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKA 204 D++G +L G+ A L FL A GLS LL RVL G++ G+F G A A+ E Sbjct: 70 DQLGRRPLLFAGLTAAALSDLVFLGAEGLSSLLAGRVLSGMSAGIFTGTATVAVVESAPV 129 Query: 205 NSKRQAAYLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLV--R 262 KR A ++A G LGPV++G LAEY WP + + VHL L+V AV+G+ + Sbjct: 130 AWKRTATFVATAANMGGLGLGPVVAGALAEYVAWPLHLAYAVHLGLLVIAVLGVAVAAET 189 Query: 263 IRSEPAGTASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXX 322 + P + +VPA +RG F A + F + V+ F + P ++ + Sbjct: 190 VTRPPRIRLRVQRLKVPAEVRGVFMPAVIAGFAGFAVLGFFTATAPAFMAQVLGHDNLLA 249 Query: 323 XXXXXXXXXXXXXXXQFTLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILL 382 Q +LP R + +L L+ Sbjct: 250 IGLVAGCVFFASTLGQLAQDRLPPARRLPLGCAVVIVGALVVGAGLALATLWGFLLGALI 309 Query: 383 VGMGHGPLYAGSLAYVNAIAPSEARGDTVSFFYTGTYIGVA 423 VG+G G + L V A P RG S F+ Y+ ++ Sbjct: 310 VGLGQGIAFRAGLGAVAAACPMNQRGAVTSTFFVIVYVAIS 350 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,134,779,169 Number of extensions: 79005138 Number of successful extensions: 305057 Number of sequences better than 1.0e-05: 662 Number of HSP's gapped: 308562 Number of HSP's successfully gapped: 662 Length of query: 442 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 303 Effective length of database: 1,751,175,118 Effective search space: 530606060754 Effective search space used: 530606060754 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)