BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0017.p (452 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5CZX4.1 A5CZX4_PELTS Glutamine synthetase 455 e-126 Q7TXP3.1 Q7TXP3_MYCBO PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTA... 373 e-101 O33342.1 O33342_MYCTU PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTA... 373 e-101 C1AFX6.1 C1AFX6_MYCBT Putative glutamine synthetase EC=6.3.1.2 373 e-101 A5U6L4.1 A5U6L4_MYCTA Putative glutamine synthetase 373 e-101 >A5CZX4.1 A5CZX4_PELTS Glutamine synthetase Length = 452 Score = 455 bits (1171), Expect = e-126, Method: Compositional matrix adjust. Identities = 236/457 (51%), Positives = 308/457 (67%), Gaps = 10/457 (2%) Query: 1 MAYLEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWD 60 M ++ L+ ++ GE+ +VIIA DMQ + FGKR F+ +A GI+ C+ NL WD Sbjct: 1 MTKIDTGILKSMIDRGEIDTVIIAAADMQSRFFGKRCCADFFLSEAVHGINICSNNLIWD 60 Query: 61 VSLNFGA-FDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRN 119 + LN G + TG+ D+ +PD++TLR YPW KT V++DL +DGT ++IAPRN Sbjct: 61 IELNEGGQYPLAGRRTGLPDLRAVPDMTTLRLYPWFEKTVLVMADLFHQDGTTVTIAPRN 120 Query: 120 MLKRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVD 179 +LKRQ+ KA +GL+ A+EIEFYIF ET+ET R+K Y L PL P + SI++ + Sbjct: 121 ILKRQVEKAGNMGLQADMAAEIEFYIFNETAETARDKRYRELLPLSRMPAE-SIHQSSRY 179 Query: 180 HWFLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALN 239 FLG++ L AG +E + E G GQ E+N+RY ALEMADRTAIYKNGIKEMA L Sbjct: 180 EHFLGKVRRYLNGAGVEVELARPERGRGQAEVNLRYTGALEMADRTAIYKNGIKEMADLE 239 Query: 240 GLMATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAP 299 L TFMAK T + S H H+SL D + GNN+ YD E+G+S T ++FLAGM+ LAP Sbjct: 240 KLAVTFMAKYKTEEPASGFHVHLSLRD-QKGNNIFYDENDEHGMSATCKHFLAGMITLAP 298 Query: 300 ELMVFYAPYINSYKRIMDFGS--GAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADA 357 ELM FYAPYINSYKR +GS GAP +SWG DNR+ SFR G+GKS R ENR+PGADA Sbjct: 299 ELMCFYAPYINSYKR---YGSTFGAPRNLSWGFDNRTLSFRVIGRGKSFRIENRIPGADA 355 Query: 358 NHYLVLSAMIASGLYGIENRLELPA-PITGNAGKL-EIPRLPTNLYDAVKILENSKTVRE 415 N YLVL+A +ASGLYGIEN LP+ P+ G+A ++ E+ LP +L DA+ ENS+T R Sbjct: 356 NPYLVLAACLASGLYGIENETALPSPPVRGSAYEMRELRVLPASLMDALADFENSETARA 415 Query: 416 LLGEEVVHHYLQAAQLELRDFFSSVTDWERKKYFEFI 452 L G EV+ HY AA+ E+ F SVTDWER++ FE + Sbjct: 416 LFGREVIDHYALAAKQEIECFLKSVTDWERRRNFEQV 452 >Q7TXP3.1 Q7TXP3_MYCBO PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTAMINE SYNTHASE) (GS-II) EC=6.3.1.2 Length = 457 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 202/450 (44%), Positives = 273/450 (60%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 L W +L R V +G+V +VI+A DMQG+L GKR+ G HF++D A G+ C+ LA DV Sbjct: 9 LAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLLAVDVD 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 LN + +WDTG DMV PDLSTLR PW P TA V++DL DG++++++PR++L Sbjct: 69 LNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVSPRSIL 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 +RQ+ + GL A+E+EF +F + Y GL P +Y +DY+I + Sbjct: 129 RRQLDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAILASSRMEP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 L + + AG EA+KGE GQ E+ RY EAL D AIYKNG KE+A +G Sbjct: 189 LLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGK 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK D + G+S H H+SL +G+ V D +G+S R F+AG L E Sbjct: 249 SLTFMAKYDERE-GNSCHIHVSLRG-TDGSAVFADSNGPHGMSSMFRSFVAGQLATLREF 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 + YAP INSYKR D S AP ++WG+DNR+ + R G G++ R E RVPG D N YL Sbjct: 307 TLCYAPTINSYKRFAD-SSFAPTALAWGLDNRTCALRVVGHGQNIRVECRVPGGDVNQYL 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 ++A+IA GLYGIE L+LP P GNA + ++ RLP L DA + E+S VRE GE+ Sbjct: 366 AVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGED 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV HYL A++EL F ++VTDWER + FE Sbjct: 426 VVAHYLNNARVELAAFNAAVTDWERIRGFE 455 >O33342.1 O33342_MYCTU PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTAMINE SYNTHASE) (GS-II) EC=6.3.1.2 Length = 457 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 202/450 (44%), Positives = 273/450 (60%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 L W +L R V +G+V +VI+A DMQG+L GKR+ G HF++D A G+ C+ LA DV Sbjct: 9 LAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLLAVDVD 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 LN + +WDTG DMV PDLSTLR PW P TA V++DL DG++++++PR++L Sbjct: 69 LNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVSPRSIL 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 +RQ+ + GL A+E+EF +F + Y GL P +Y +DY+I + Sbjct: 129 RRQLDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAILASSRMEP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 L + + AG EA+KGE GQ E+ RY EAL D AIYKNG KE+A +G Sbjct: 189 LLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGK 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK D + G+S H H+SL +G+ V D +G+S R F+AG L E Sbjct: 249 SLTFMAKYDERE-GNSCHIHVSLRG-TDGSAVFADSNGPHGMSSMFRSFVAGQLATLREF 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 + YAP INSYKR D S AP ++WG+DNR+ + R G G++ R E RVPG D N YL Sbjct: 307 TLCYAPTINSYKRFAD-SSFAPTALAWGLDNRTCALRVVGHGQNIRVECRVPGGDVNQYL 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 ++A+IA GLYGIE L+LP P GNA + ++ RLP L DA + E+S VRE GE+ Sbjct: 366 AVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGED 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV HYL A++EL F ++VTDWER + FE Sbjct: 426 VVAHYLNNARVELAAFNAAVTDWERIRGFE 455 >C1AFX6.1 C1AFX6_MYCBT Putative glutamine synthetase EC=6.3.1.2 Length = 457 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 202/450 (44%), Positives = 273/450 (60%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 L W +L R V +G+V +VI+A DMQG+L GKR+ G HF++D A G+ C+ LA DV Sbjct: 9 LAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLLAVDVD 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 LN + +WDTG DMV PDLSTLR PW P TA V++DL DG++++++PR++L Sbjct: 69 LNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVSPRSIL 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 +RQ+ + GL A+E+EF +F + Y GL P +Y +DY+I + Sbjct: 129 RRQLDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAILASSRMEP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 L + + AG EA+KGE GQ E+ RY EAL D AIYKNG KE+A +G Sbjct: 189 LLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGK 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK D + G+S H H+SL +G+ V D +G+S R F+AG L E Sbjct: 249 SLTFMAKYDERE-GNSCHIHVSLRG-TDGSAVFADSNGPHGMSSMFRSFVAGQLATLREF 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 + YAP INSYKR D S AP ++WG+DNR+ + R G G++ R E RVPG D N YL Sbjct: 307 TLCYAPTINSYKRFAD-SSFAPTALAWGLDNRTCALRVVGHGQNIRVECRVPGGDVNQYL 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 ++A+IA GLYGIE L+LP P GNA + ++ RLP L DA + E+S VRE GE+ Sbjct: 366 AVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGED 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV HYL A++EL F ++VTDWER + FE Sbjct: 426 VVAHYLNNARVELAAFNAAVTDWERIRGFE 455 >A5U6L4.1 A5U6L4_MYCTA Putative glutamine synthetase Length = 457 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 202/450 (44%), Positives = 273/450 (60%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 L W +L R V +G+V +VI+A DMQG+L GKR+ G HF++D A G+ C+ LA DV Sbjct: 9 LAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLLAVDVD 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 LN + +WDTG DMV PDLSTLR PW P TA V++DL DG++++++PR++L Sbjct: 69 LNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVSPRSIL 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 +RQ+ + GL A+E+EF +F + Y GL P +Y +DY+I + Sbjct: 129 RRQLDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAILASSRMEP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 L + + AG EA+KGE GQ E+ RY EAL D AIYKNG KE+A +G Sbjct: 189 LLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGK 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK D + G+S H H+SL +G+ V D +G+S R F+AG L E Sbjct: 249 SLTFMAKYDERE-GNSCHIHVSLRG-TDGSAVFADSNGPHGMSSMFRSFVAGQLATLREF 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 + YAP INSYKR D S AP ++WG+DNR+ + R G G++ R E RVPG D N YL Sbjct: 307 TLCYAPTINSYKRFAD-SSFAPTALAWGLDNRTCALRVVGHGQNIRVECRVPGGDVNQYL 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 ++A+IA GLYGIE L+LP P GNA + ++ RLP L DA + E+S VRE GE+ Sbjct: 366 AVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGED 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV HYL A++EL F ++VTDWER + FE Sbjct: 426 VVAHYLNNARVELAAFNAAVTDWERIRGFE 455 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,801,123,147 Number of extensions: 121104271 Number of successful extensions: 278695 Number of sequences better than 1.0e-05: 3146 Number of HSP's gapped: 267855 Number of HSP's successfully gapped: 3156 Length of query: 452 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 313 Effective length of database: 1,751,175,118 Effective search space: 548117811934 Effective search space used: 548117811934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)