BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0007.p (297 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KD14.1 B3KD14_9BACI L-lactate dehydrogenase 416 e-114 B8I648.1 B8I648_CLOCE L-lactate dehydrogenase 320 1e-85 B9MKZ8.1 B9MKZ8_ANATD L-lactate dehydrogenase 312 3e-83 B4BHS7.1 B4BHS7_CLOTM L-lactate dehydrogenase 311 6e-83 Q8KQC4.2 LDH_CLOTH L-lactate dehydrogenase EC=1.1.1.27 309 2e-82 >B3KD14.1 B3KD14_9BACI L-lactate dehydrogenase Length = 310 Score = 416 bits (1068), Expect = e-114, Method: Compositional matrix adjust. Identities = 196/275 (71%), Positives = 235/275 (85%) Query: 21 MQELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGET 80 + ELVLID NH KALGEALDMNHG+PF GGVKLWAG Y DC++ADII++ AG++Q+PGET Sbjct: 34 VSELVLIDANHDKALGEALDMNHGLPFAGGVKLWAGDYSDCKDADIIVIAAGSNQRPGET 93 Query: 81 RIDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVL 140 RIDLL++N +IF DIIQ I KYN H I+L+ATNPVDIL+Y TLK SGF RVIGSGT+L Sbjct: 94 RIDLLKRNTAIFDDIIQNIVKYNDHGIILVATNPVDILSYVTLKKSGFPVNRVIGSGTLL 153 Query: 141 DSARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDLGFDDAERKEIFEDT 200 DSARFRYLIG +K I+PRSIH I+GEHGDSELP+WS+AN+AGI + D ER++IF+ T Sbjct: 154 DSARFRYLIGQNKGINPRSIHAHIVGEHGDSELPLWSIANIAGIGIDLTDDEREDIFDRT 213 Query: 201 KNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDVFLGAPCVV 260 KNAAYEII+AKGSTSYAIALALDRI+VSIL NEG+VLNVSTLL +Y+GVSDV+LG PCVV Sbjct: 214 KNAAYEIINAKGSTSYAIALALDRIIVSILQNEGSVLNVSTLLTDYHGVSDVYLGVPCVV 273 Query: 261 DRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKL 295 DR+GVRE+L L +++E T F SA KLK +ISK+ Sbjct: 274 DRTGVREILSLEFNDEELTQFHASANKLKEQISKI 308 >B8I648.1 B8I648_CLOCE L-lactate dehydrogenase Length = 316 Score = 320 bits (819), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 156/286 (54%), Positives = 204/286 (71%), Gaps = 9/286 (3%) Query: 21 MQELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGET 80 + E+VLID N KA GEA+DMNHGMPFV V+++ G Y DC+ ADI+++T GA+QKPGET Sbjct: 31 VSEIVLIDRNTSKAEGEAMDMNHGMPFVRPVRIYKGDYPDCKGADIVVITGGANQKPGET 90 Query: 81 RIDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVL 140 RIDL+ KN +FKDI+ I KYN ILL+ TNPVDIL Y T K+SGF + RVIGSGTVL Sbjct: 91 RIDLVNKNTEVFKDIVGNIIKYNTDCILLVVTNPVDILTYVTYKLSGFPKNRVIGSGTVL 150 Query: 141 DSARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGI-------DLGFDDAE- 192 D+ARF+Y++G H +DPR++H IIGEHGD+E+P WSLA++AGI + DAE Sbjct: 151 DTARFKYMLGEHMGVDPRNVHAYIIGEHGDTEVPTWSLASIAGIPMDAYCKECKSCDAEN 210 Query: 193 -RKEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSD 251 + E F+ KNAAYEIID K +T YA+ALA+ RIV +I+ NE ++L VS+L G++D Sbjct: 211 FKSETFDKVKNAAYEIIDRKNATYYAVALAVRRIVEAIVRNENSILTVSSLFEGEYGLND 270 Query: 252 VFLGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLEL 297 + L P V+ GV +L++PLS +E L KSA+ LK IS L L Sbjct: 271 ICLSIPSQVNSEGVSRILNIPLSSEETGLLNKSAQALKQVISGLNL 316 >B9MKZ8.1 B9MKZ8_ANATD L-lactate dehydrogenase Length = 314 Score = 312 bits (799), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 150/284 (52%), Positives = 206/284 (72%), Gaps = 9/284 (3%) Query: 23 ELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGETRI 82 ELVLIDVN KA GEA+D+NHG+ FV VK+WAG YEDC++ADIII+TAGA+QKPGETR+ Sbjct: 31 ELVLIDVNRAKAEGEAMDLNHGISFVKPVKIWAGDYEDCKDADIIIITAGANQKPGETRL 90 Query: 83 DLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVLDS 142 DL KN I K II+ I KY H AILL+ TNPVD+L Y K+SG + +VIGSGTVLDS Sbjct: 91 DLTYKNAQITKSIIENIIKYTHDAILLMVTNPVDVLTYVMYKVSGLPKNQVIGSGTVLDS 150 Query: 143 ARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGIDL---------GFDDAER 193 +RFRYL+ H ++D R++H I+GEHGDSE+ WSL N+ G++ + Sbjct: 151 SRFRYLLAQHCQVDVRNVHAYILGEHGDSEIAAWSLTNIGGVNFMQECLLCGKNCSPEVK 210 Query: 194 KEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSDVF 253 ++IF KNAAYEII+ KG+T YAIALA+ RIV +I+ +E ++L VS+++++ GV DV Sbjct: 211 EQIFNKVKNAAYEIIERKGATYYAIALAVRRIVEAIIRDENSILPVSSIVDDVYGVKDVA 270 Query: 254 LGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLEL 297 + P ++++SGV +V D+PL+++EK + SA+ +KS I L+L Sbjct: 271 ISLPAIINKSGVVKVFDIPLTDEEKEKLKNSAQVIKSVIESLKL 314 >B4BHS7.1 B4BHS7_CLOTM L-lactate dehydrogenase Length = 317 Score = 311 bits (796), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 151/286 (52%), Positives = 208/286 (72%), Gaps = 9/286 (3%) Query: 21 MQELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGET 80 + E+VLID+N +KA GE +D+NHGMPFV VK++ G Y+DC +DI+I+TAGA+QK GET Sbjct: 32 ISEIVLIDINAKKADGEVMDLNHGMPFVRPVKIYRGDYKDCAGSDIVIITAGANQKEGET 91 Query: 81 RIDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVL 140 RIDL+++N +FK+II +I KYN+ ILL+ TNPVDIL Y T K+SGF + +VIGSGTVL Sbjct: 92 RIDLVKRNTEVFKNIINEIVKYNNDCILLVVTNPVDILTYVTYKLSGFPKNKVIGSGTVL 151 Query: 141 DSARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGI--DLGFDDA------- 191 D+ARFRYL+ H ++D R++H IIGEHGD+E+ WSLAN+AGI D D+ Sbjct: 152 DTARFRYLLSEHVKVDARNVHAYIIGEHGDTEVAAWSLANIAGIPMDRYCDECHQCEEQI 211 Query: 192 ERKEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSD 251 R +I+E KNAAYEII KG+T YA+ALA+ RIV +I+ NE ++L VS+LL G+SD Sbjct: 212 SRNKIYESVKNAAYEIIRNKGATYYAVALAVRRIVEAIVRNENSILTVSSLLEGQYGLSD 271 Query: 252 VFLGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLEL 297 V L P +V +G+ E+L++P +++E L +KS LK I L++ Sbjct: 272 VCLSVPTIVGVNGIEEILNVPFNDEEIQLLRKSGNTLKEIIKTLDI 317 >Q8KQC4.2 LDH_CLOTH L-lactate dehydrogenase EC=1.1.1.27 Length = 317 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 150/286 (52%), Positives = 208/286 (72%), Gaps = 9/286 (3%) Query: 21 MQELVLIDVNHQKALGEALDMNHGMPFVGGVKLWAGTYEDCREADIIIVTAGASQKPGET 80 + E+VLID+N +KA GE +D+NHGMPFV V+++ G Y+DC +DI+I+TAGA+QK GET Sbjct: 32 ISEIVLIDINAKKADGEVMDLNHGMPFVRPVEIYRGDYKDCAGSDIVIITAGANQKEGET 91 Query: 81 RIDLLRKNISIFKDIIQKITKYNHHAILLIATNPVDILAYATLKISGFDRRRVIGSGTVL 140 RIDL+++N +FK+II +I KYN+ ILL+ TNPVDIL Y T K+SGF + +VIGSGTVL Sbjct: 92 RIDLVKRNTEVFKNIINEIVKYNNDCILLVVTNPVDILTYVTYKLSGFPKNKVIGSGTVL 151 Query: 141 DSARFRYLIGLHKEIDPRSIHGQIIGEHGDSELPVWSLANVAGI--DLGFDDA------- 191 D+ARFRYL+ H ++D R++H IIGEHGD+E+ WSLAN+AGI D D+ Sbjct: 152 DTARFRYLLSEHVKVDARNVHAYIIGEHGDTEVAAWSLANIAGIPMDRYCDECHQCEEQI 211 Query: 192 ERKEIFEDTKNAAYEIIDAKGSTSYAIALALDRIVVSILHNEGAVLNVSTLLNNYNGVSD 251 R +I+E KNAAYEII KG+T YA+ALA+ RIV +I+ NE ++L VS+LL G+SD Sbjct: 212 SRNKIYESVKNAAYEIIRNKGATYYAVALAVRRIVEAIVRNENSILTVSSLLEGQYGLSD 271 Query: 252 VFLGAPCVVDRSGVREVLDLPLSEDEKTLFQKSAEKLKSEISKLEL 297 V L P +V +G+ E+L++P +++E L +KS LK I L++ Sbjct: 272 VCLSVPTIVGVNGIEEILNVPFNDEEIQLLRKSGNTLKEIIKTLDI 317 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,588,998,939 Number of extensions: 66489590 Number of successful extensions: 184700 Number of sequences better than 1.0e-05: 3722 Number of HSP's gapped: 180205 Number of HSP's successfully gapped: 3722 Length of query: 297 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 162 Effective length of database: 1,788,859,178 Effective search space: 289795186836 Effective search space used: 289795186836 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)