BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0001.p (179 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z9P8.1 C0Z9P8_BREBN Putative lipase 286 6e-76 B1D8J6.1 B1D8J6_9BACL Alpha/beta hydrolase fold-3 domain protein 281 2e-74 A3KKG0.1 A3KKG0_STRAM Putative lipase 260 3e-68 C1WVX7.1 C1WVX7_9ACTO Esterase/lipase 258 1e-67 B4V5S9.1 B4V5S9_9ACTO Putative uncharacterized protein 253 7e-66 >C0Z9P8.1 C0Z9P8_BREBN Putative lipase Length = 357 Score = 286 bits (732), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 133/178 (74%), Positives = 151/178 (84%) Query: 2 LTWIAEHGSEYGLDTSKLTVAGDSVGGNMTAAITLMAKERGGPAISKQLLFYPVTDASFD 61 L W++ +G ++G++ KL VAGDSVGGNMTAAITLMAKER GP I+KQLLFYPVTDASFD Sbjct: 179 LEWVSRNGKQHGMNPEKLAVAGDSVGGNMTAAITLMAKERKGPQINKQLLFYPVTDASFD 238 Query: 62 TESYHLFAEGYFLQRTGMKWFWDQYTTSEEERAQITASPLRASLEQLSGLPEALIITAEA 121 T SYH FA+ YFL+R M+WFWDQYTT ++RA+ITASPLRA+ EQL GLPEALIITAEA Sbjct: 239 TPSYHQFAKNYFLRRDAMQWFWDQYTTDPKQRAEITASPLRATTEQLKGLPEALIITAEA 298 Query: 122 DVLRDEGEAYANKLREAGVAVTAVRFQGIIHDFVMLNALAETNAKKGALLLATNWLRQ 179 DVLRDEGEAY NKLREAGV VT VRFQGIIHDFVMLN LA T A +GA+ LA WL+Q Sbjct: 299 DVLRDEGEAYGNKLREAGVPVTTVRFQGIIHDFVMLNDLANTEAARGAMTLANAWLKQ 356 >B1D8J6.1 B1D8J6_9BACL Alpha/beta hydrolase fold-3 domain protein Length = 318 Score = 281 bits (719), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 136/178 (76%), Positives = 151/178 (84%) Query: 2 LTWIAEHGSEYGLDTSKLTVAGDSVGGNMTAAITLMAKERGGPAISKQLLFYPVTDASFD 61 L W+AE+G E+GLDTS+L VAGDSVGGNMTAAITLMAKER GP I KQLLFYPVTDA D Sbjct: 141 LLWMAENGEEHGLDTSRLYVAGDSVGGNMTAAITLMAKERKGPQIQKQLLFYPVTDAGND 200 Query: 62 TESYHLFAEGYFLQRTGMKWFWDQYTTSEEERAQITASPLRASLEQLSGLPEALIITAEA 121 TESY FA GYFL+R M WFWDQYTT+ EER +I ASPLRA+ EQL GLP+ALIIT EA Sbjct: 201 TESYKEFATGYFLRRDAMAWFWDQYTTNREEREEIYASPLRATTEQLKGLPQALIITGEA 260 Query: 122 DVLRDEGEAYANKLREAGVAVTAVRFQGIIHDFVMLNALAETNAKKGALLLATNWLRQ 179 DVLRDEGEAYANKLREAGV VT RFQG IHDFVMLNALA+T+A +GA+LLA WL++ Sbjct: 261 DVLRDEGEAYANKLREAGVRVTQARFQGTIHDFVMLNALADTSAARGAMLLANAWLKE 318 >A3KKG0.1 A3KKG0_STRAM Putative lipase Length = 330 Score = 260 bits (665), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 123/164 (75%), Positives = 141/164 (85%) Query: 1 VLTWIAEHGSEYGLDTSKLTVAGDSVGGNMTAAITLMAKERGGPAISKQLLFYPVTDASF 60 V WI + G+ LD S+L VAGDSVGGNM AA+TLMAKERG + +Q+LFYPVTDA+F Sbjct: 140 VARWIVQEGAASALDGSRLAVAGDSVGGNMAAALTLMAKERGDVPLVQQVLFYPVTDAAF 199 Query: 61 DTESYHLFAEGYFLQRTGMKWFWDQYTTSEEERAQITASPLRASLEQLSGLPEALIITAE 120 DT SYH FAEGYFL+R GM+WFWDQYTT ERA+ITASPLRA+ EQL+GLP AL++TAE Sbjct: 200 DTASYHQFAEGYFLRRDGMQWFWDQYTTDPAERARITASPLRATTEQLTGLPPALVVTAE 259 Query: 121 ADVLRDEGEAYANKLREAGVAVTAVRFQGIIHDFVMLNALAETN 164 ADVLRDEGEAYANKLR+AGVAVTAVRFQG+IHDFVMLNAL ET+ Sbjct: 260 ADVLRDEGEAYANKLRQAGVAVTAVRFQGVIHDFVMLNALRETH 303 >C1WVX7.1 C1WVX7_9ACTO Esterase/lipase Length = 309 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 128/178 (71%), Positives = 142/178 (79%) Query: 1 VLTWIAEHGSEYGLDTSKLTVAGDSVGGNMTAAITLMAKERGGPAISKQLLFYPVTDASF 60 V WI G GLD ++L +AGDSVGGNM AA+TL+AKERG +Q+LFYPVTDASF Sbjct: 128 VARWIVAEGKSKGLDATRLAIAGDSVGGNMAAAVTLLAKERGDLTFRQQVLFYPVTDASF 187 Query: 61 DTESYHLFAEGYFLQRTGMKWFWDQYTTSEEERAQITASPLRASLEQLSGLPEALIITAE 120 DT SY FAEGYFL R GM+WFWDQYTT E ER QITASPLRA+ EQLSGLP AL+ITAE Sbjct: 188 DTTSYQEFAEGYFLMREGMQWFWDQYTTDEAERNQITASPLRATTEQLSGLPTALVITAE 247 Query: 121 ADVLRDEGEAYANKLREAGVAVTAVRFQGIIHDFVMLNALAETNAKKGALLLATNWLR 178 ADVLRDEGEAYA KLREAGVAVTAVRF GIIHDFVML+AL +T+A A+ LA LR Sbjct: 248 ADVLRDEGEAYAAKLREAGVAVTAVRFGGIIHDFVMLDALRDTHAATAAIDLAVRTLR 305 >B4V5S9.1 B4V5S9_9ACTO Putative uncharacterized protein Length = 317 Score = 253 bits (645), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 124/179 (69%), Positives = 144/179 (80%) Query: 1 VLTWIAEHGSEYGLDTSKLTVAGDSVGGNMTAAITLMAKERGGPAISKQLLFYPVTDASF 60 V W+A G LD +++ VAGDSVGGNM+AA+TLMAK+RG I Q+LFYPVTDA+F Sbjct: 136 VAQWVAREGRHKDLDGTRIAVAGDSVGGNMSAALTLMAKQRGDVNIVHQVLFYPVTDAAF 195 Query: 61 DTESYHLFAEGYFLQRTGMKWFWDQYTTSEEERAQITASPLRASLEQLSGLPEALIITAE 120 DTESY FA GYFL+R M+WFWDQYTT E ERAQITASPLRASL+QL+GLP AL+ITAE Sbjct: 196 DTESYRQFATGYFLRRDAMRWFWDQYTTDEAERAQITASPLRASLDQLTGLPPALVITAE 255 Query: 121 ADVLRDEGEAYANKLREAGVAVTAVRFQGIIHDFVMLNALAETNAKKGALLLATNWLRQ 179 ADVLRDEGEAYA KLR AGV VTA+R QG IHDFVMLN L ET+A + A+ LA + LR+ Sbjct: 256 ADVLRDEGEAYAAKLRAAGVPVTALRVQGTIHDFVMLNPLRETHAAELAIGLAADTLRK 314 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,011,116,555 Number of extensions: 37509155 Number of successful extensions: 114327 Number of sequences better than 1.0e-05: 1442 Number of HSP's gapped: 113107 Number of HSP's successfully gapped: 1442 Length of query: 179 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 52 Effective length of database: 1,864,227,298 Effective search space: 96939819496 Effective search space used: 96939819496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)