BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0056.p (310 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1DGK6.1 C1DGK6_AZOVD Monoxygenase, NtaA/DszA family 309 2e-82 A4IU28.1 A4IU28_GEOTN Alkane monoxygenase 303 1e-80 Q1IGN2.1 Q1IGN2_PSEE4 Putative monooxygenase 302 3e-80 A8DC15.1 A8DC15_BACST Alkane monooxygenase 302 3e-80 Q4KDF9.1 Q4KDF9_PSEF5 Monooxygenase, NtaA/SnaA/SoxA family 302 3e-80 >C1DGK6.1 C1DGK6_AZOVD Monoxygenase, NtaA/DszA family Length = 467 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 154/306 (50%), Positives = 212/306 (69%), Gaps = 3/306 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HDERYD ADEY+E+ YKL EGSWED AV DRE R+FTDP+KVH+I H G++F+++G H Sbjct: 154 HDERYDHADEYLEVIYKLLEGSWEDGAVIRDRERRLFTDPAKVHEIDHRGEHFQVSGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV+FQAG+S RGR F+A+HAE VF+ AP+ VL++ + DIR+RA E GRDP Sbjct: 214 CEPSPQRTPVLFQAGASSRGRRFSAEHAECVFVAAPSKAVLKKTVADIRQRAVEAGRDPY 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 +++ +V++G T EA+ K +Y Y S E L+ + TG+D + PD ++IH Sbjct: 274 QIRILNLQTVILGETDAEAQAKWRDYKQYVSYEGALALISGWTGLDFGQYQPDQVLKHIH 333 Query: 185 TEMGQTHTDRFTKHSKTKK-TVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVD 243 T Q+ + F+ +K+ TVRE+ D++ G G +G+P+ +ADQ+Q WVEETDVD Sbjct: 334 TNAIQSAVEAFSTADPSKQWTVRELA-DWVGIGGFGPLFVGSPQTVADQLQEWVEETDVD 392 Query: 244 GFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVF 303 GFNL V ++ D V+L+VP LQ+RG+FK +Y GT REKLFG G RLP++HPG + Sbjct: 393 GFNLAYAVSHETFVDVVELLVPELQKRGVFKTDYAPGTLREKLFGAG-PRLPDNHPGARY 451 Query: 304 RNPATL 309 R+ A L Sbjct: 452 RDLAAL 457 >A4IU28.1 A4IU28_GEOTN Alkane monoxygenase Length = 440 Score = 303 bits (776), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 150/308 (48%), Positives = 204/308 (66%), Gaps = 21/308 (6%) Query: 2 FLPHDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTG 61 L HDERYD ADEY+E+ YKL+EGSWED+AV D EN ++TDPSKVH+I H GKYF++ G Sbjct: 151 ILEHDERYDLADEYLEVCYKLWEGSWEDNAVIRDIENNIYTDPSKVHEINHSGKYFEVPG 210 Query: 62 YHSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGR 121 H EPS QRTPVI+QAG S+RGR FAAKHAE VFL E L+ + DIR+RA++YGR Sbjct: 211 PHLCEPSPQRTPVIYQAGMSERGREFAAKHAECVFLGGKDVETLKFFVDDIRKRAKKYGR 270 Query: 122 DPAAVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFE 181 +P +K+F G+ V+VG+T +EA K + Y S E L+ Y TG DL+K + Y Sbjct: 271 NPDHIKMFAGICVIVGKTHDEAMEKLNSFQKYWSLEGHLAHYGGGTGYDLSKYSSNDYIG 330 Query: 182 NIHTEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTV-IGTPEEIADQIQYWVEET 240 +I +V E+ + K + + +GTP+++AD++QY VEE Sbjct: 331 SI--------------------SVGEIINNMSKLDGKWFKLSVGTPKKVADEMQYLVEEA 370 Query: 241 DVDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPG 300 +DGFNL YV P ++ DF++LVVP LQ+RG+++ +YEEGT+REKLFGKG RLP+ H Sbjct: 371 GIDGFNLVQYVSPGTFVDFIELVVPELQKRGLYRVDYEEGTYREKLFGKGNYRLPDDHIA 430 Query: 301 TVFRNPAT 308 +RN ++ Sbjct: 431 ARYRNISS 438 >Q1IGN2.1 Q1IGN2_PSEE4 Putative monooxygenase Length = 463 Score = 302 bits (773), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 147/302 (48%), Positives = 198/302 (65%), Gaps = 4/302 (1%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD ADEY+++ YKL EGSW+DDAV DRE RV+ P KVHK++H G+++K+ GYH Sbjct: 154 HDRRYDQADEYLQVLYKLLEGSWDDDAVVADREQRVYARPDKVHKVEHHGEFYKVEGYHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV+FQAGSS RG AFA HAE VF+ R Q+ +R A+ GRDP Sbjct: 214 CEPSPQRTPVLFQAGSSARGLAFAGNHAECVFISGQDKPSTRAQVDKVRAAAKAAGRDPQ 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 AVKVF GL+V+V T+E+A KH EYL + SAEA ++ +A+ TGID ++ + D Sbjct: 274 AVKVFMGLTVIVAPTEEQARAKHAEYLRHASAEAGVAHFASSTGIDFSRYELDEPIGFAK 333 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 Q+ T + + T++ + E G R +T++G PE++A+Q+ W++ET +DG Sbjct: 334 GNAIQSATRQLQDSAWTRRRLLEQH----ALGGRYVTLVGAPEQVAEQLIAWIDETGLDG 389 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL V P SYEDF+DLV+P LQ+RG +K Y EG+ R+KLF A LP HPG +R Sbjct: 390 FNLARTVTPESYEDFIDLVIPELQRRGRYKTAYAEGSLRQKLFATPHAHLPADHPGAAYR 449 Query: 305 NP 306 NP Sbjct: 450 NP 451 >A8DC15.1 A8DC15_BACST Alkane monooxygenase Length = 440 Score = 302 bits (773), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 150/308 (48%), Positives = 204/308 (66%), Gaps = 21/308 (6%) Query: 2 FLPHDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTG 61 L HDERYD ADEY+E+ YKL+EGSWED+AV D EN ++TDPSKVH+I H GKYF++ G Sbjct: 151 ILEHDERYDLADEYLEVCYKLWEGSWEDNAVIRDIENNIYTDPSKVHEINHSGKYFEVPG 210 Query: 62 YHSSEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGR 121 H EPS QRTPVI+QAG S+RGR FAAKHAE VFL E L+ + DIR+RA++YGR Sbjct: 211 PHLCEPSPQRTPVIYQAGMSERGREFAAKHAETVFLGGTDVETLKFFVDDIRKRAKKYGR 270 Query: 122 DPAAVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFE 181 +P +K+F G+ V+VG+T +EA K + Y S E L+ Y TG DL+K + Y Sbjct: 271 NPDHIKMFAGICVIVGKTHDEAMEKLNSFQKYWSLEGHLAHYGGGTGYDLSKYSSNDYIG 330 Query: 182 NIHTEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTV-IGTPEEIADQIQYWVEET 240 +I +V E+ + K + + +GTP+++AD++QY VEE Sbjct: 331 SI--------------------SVGEIINNMSKLDGKWFKLSVGTPKKVADEMQYLVEEA 370 Query: 241 DVDGFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPG 300 +DGFNL YV P ++ DF++LVVP LQ+RG+++ +YEEGT+REKLFGKG RLP+ H Sbjct: 371 GIDGFNLVQYVSPGTFVDFIELVVPELQKRGLYRVDYEEGTYREKLFGKGNYRLPDDHIA 430 Query: 301 TVFRNPAT 308 +RN ++ Sbjct: 431 ARYRNISS 438 >Q4KDF9.1 Q4KDF9_PSEF5 Monooxygenase, NtaA/SnaA/SoxA family Length = 467 Score = 302 bits (773), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 145/301 (48%), Positives = 204/301 (67%), Gaps = 1/301 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD A+EY+E+ YKL+EGSWED A+ DRE R+F+DPSK+H+I+H GK+F++ G H Sbjct: 154 HDARYDYAEEYLEVCYKLWEGSWEDGAILRDRERRIFSDPSKIHEIRHVGKHFQVPGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV++QAG+S RG+ FAA+ AE VF+ AP+ +L++ + DIRRRA E GRDP+ Sbjct: 214 CEPSPQRTPVLYQAGASSRGKQFAAEQAECVFVAAPSKVLLKKTVADIRRRAAEAGRDPS 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 + +F +V++G T +A+ K EY S+ S E ++ + TGID ++ PD +++H Sbjct: 274 KILIFNLQTVILGETDAKAKAKFEEYKSWVSYEGAMALISGWTGIDFSRFKPDEPLKHVH 333 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 T Q+ + F+ K D++ G G +G+PE +AD +Q WVEETDVDG Sbjct: 334 TNAIQSAVEAFSTADPNKVWTPHELADWVGIGGFGPLFVGSPETVADLLQEWVEETDVDG 393 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL + S+ D V+L+VP LQ+RG++K EY GT REKLFG G RLPE HPG+ +R Sbjct: 394 FNLAYALTHESFIDAVELLVPELQKRGVYKTEYAPGTLREKLFGAG-PRLPESHPGSAWR 452 Query: 305 N 305 N Sbjct: 453 N 453 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,938,207,757 Number of extensions: 82957191 Number of successful extensions: 224017 Number of sequences better than 1.0e-05: 1088 Number of HSP's gapped: 222747 Number of HSP's successfully gapped: 1103 Length of query: 310 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 175 Effective length of database: 1,788,859,178 Effective search space: 313050356150 Effective search space used: 313050356150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)