BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0048.p (410 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D8Y0.1 C5D8Y0_9BACI Amidohydrolase EC=3.5.1.14 443 e-122 C2SFL7.1 C2SFL7_BACCE Aminoacylase (N-acyl-L-amino acid amidohyd... 438 e-121 C2PRF5.1 C2PRF5_BACCE Aminoacylase (N-acyl-L-amino acid amidohyd... 438 e-121 C3A1I9.1 C3A1I9_BACMY Aminoacylase (N-acyl-L-amino acid amidohyd... 438 e-121 C2YM77.1 C2YM77_BACCE Aminoacylase (N-acyl-L-amino acid amidohyd... 437 e-121 >C5D8Y0.1 C5D8Y0_9BACI Amidohydrolase EC=3.5.1.14 Length = 394 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 205/364 (56%), Positives = 264/364 (72%) Query: 14 ELQEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPG 73 E++E+++AWRRHLH NPELSF E TA +V + L+ G L++SRPT TSV+ARLIG +PG Sbjct: 12 EIKEEVIAWRRHLHANPELSFHEEKTAQFVYETLQSFGNLQLSRPTKTSVMARLIGDEPG 71 Query: 74 KTLALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEI 133 K +A+RADMDALPI EEN FEFAS PGVMHACGHDGHT++LLG +L+ R Q+ GE+ Sbjct: 72 KVVAIRADMDALPIQEENTFEFASKNPGVMHACGHDGHTAMLLGTAKILSRLRPQIKGEV 131 Query: 134 RFIFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTI 193 RF+FQHAEE D ++G HL + L G+IG+ YG MMASPD F I Sbjct: 132 RFLFQHAEELHPGGAEEMVQAGVMDGVDVVIGTHLWAPLETGKIGIVYGPMMASPDRFFI 191 Query: 194 VVKGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIP 253 + G GGH PH+++D IAIGAQ+V+NLQHI SRNT+PL+ LVVS+TQF G++HNVIP Sbjct: 192 RIHGKGGHAALPHQTIDSIAIGAQVVTNLQHIVSRNTDPLEPLVVSVTQFIGGTTHNVIP 251 Query: 254 DTATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLA 313 + ++GTVRSFD +R++ + + RI++GIT AHGA YE Y GY PV+ND +TR+ Sbjct: 252 GSVEIQGTVRSFDKTLRQNVPKLMERIIKGITEAHGATYEFKYEFGYRPVINDEKVTRVI 311 Query: 314 EEAAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFD 373 EE E+ G + + P M GEDFSA+QQ+ PG+F +GAGN +KGI+YPHHHPRFT D Sbjct: 312 EETVREVFGEEAIDHIKPNMGGEDFSAFQQKAPGSFFYVGAGNKEKGIVYPHHHPRFTID 371 Query: 374 EAAL 377 E AL Sbjct: 372 EDAL 375 >C2SFL7.1 C2SFL7_BACCE Aminoacylase (N-acyl-L-amino acid amidohydrolase) Length = 399 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 209/362 (57%), Positives = 266/362 (73%) Query: 16 QEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGKT 75 + QL+ WRRH H PELSF+E T+ +V LREI LE+SRPT SV+ARLIG +PGKT Sbjct: 19 KNQLIEWRRHFHKYPELSFQEEKTSQFVFDILREIPYLEVSRPTKYSVMARLIGRQPGKT 78 Query: 76 LALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIRF 135 +A+RADMDALPIHEEN+F+F S PGVMHACGHDGH +ILLGVV L +R ++ GEIRF Sbjct: 79 IAVRADMDALPIHEENKFDFISIYPGVMHACGHDGHMAILLGVVHKLVEEREKVKGEIRF 138 Query: 136 IFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIVV 195 +FQHAEE DYI+G HL + L +G++GV YG MA+PD F I + Sbjct: 139 LFQHAEENFPGGAEEMVAAGVMTGVDYIIGAHLWASLEVGKVGVIYGPAMAAPDVFKITI 198 Query: 196 KGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPDT 255 +G GGH G PH++VD IAIGAQ+VS +Q I SR TNPLD LVVS+TQF SG++HNVIP+ Sbjct: 199 EGKGGHAGIPHETVDSIAIGAQVVSQIQQIVSRLTNPLDSLVVSVTQFHSGTTHNVIPEQ 258 Query: 256 ATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAEE 315 A ++GTVRS ++R++ +++L RIV+ IT ++GA+Y Y GY PVVND+ +T L E Sbjct: 259 AEIEGTVRSLRHELREETKKKLERIVKHITESYGAKYTFSYEYGYRPVVNDYEVTELIEH 318 Query: 316 AAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDEA 375 A++L GR+ VV+ P M+GEDFSA+ Q+ PGTF IGAGN +KGI+YPHHHPRFT DE Sbjct: 319 TALQLYGRERVVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNEEKGIIYPHHHPRFTIDED 378 Query: 376 AL 377 AL Sbjct: 379 AL 380 >C2PRF5.1 C2PRF5_BACCE Aminoacylase (N-acyl-L-amino acid amidohydrolase) Length = 399 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 209/362 (57%), Positives = 266/362 (73%) Query: 16 QEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGKT 75 + QL+ WRRH H PELSF+E T+ +V LREI LE+SRPT SV+ARLIG +PGKT Sbjct: 19 KNQLIEWRRHFHKYPELSFQEEKTSQFVFDILREIPYLEVSRPTKYSVMARLIGRQPGKT 78 Query: 76 LALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIRF 135 +A+RADMDALPIHEEN+F+F S PGVMHACGHDGH +ILLGVV L +R ++ GEIRF Sbjct: 79 IAVRADMDALPIHEENKFDFISIYPGVMHACGHDGHMAILLGVVHKLVEEREKVKGEIRF 138 Query: 136 IFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIVV 195 +FQHAEE DYI+G HL + L +G++GV YG MA+PD F I + Sbjct: 139 LFQHAEENFPGGAEEMVAAGVMTGVDYIIGAHLWASLEVGKVGVIYGPAMAAPDVFKITI 198 Query: 196 KGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPDT 255 +G GGH G PH++VD IAIGAQ+VS +Q I SR TNPLD LVVS+TQF SG++HNVIP+ Sbjct: 199 EGKGGHAGIPHETVDSIAIGAQVVSQIQQIVSRLTNPLDSLVVSVTQFHSGTTHNVIPEQ 258 Query: 256 ATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAEE 315 A ++GTVRS ++R++ +++L RIV+ IT ++GA+Y Y GY PVVND+ +T L E Sbjct: 259 AEIEGTVRSLRHELREETKKKLERIVKHITESYGAKYTFSYEYGYRPVVNDYEVTELIEH 318 Query: 316 AAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDEA 375 A++L GR+ VV+ P M+GEDFSA+ Q+ PGTF IGAGN +KGI+YPHHHPRFT DE Sbjct: 319 TALQLYGRERVVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNEEKGIIYPHHHPRFTIDED 378 Query: 376 AL 377 AL Sbjct: 379 AL 380 >C3A1I9.1 C3A1I9_BACMY Aminoacylase (N-acyl-L-amino acid amidohydrolase) Length = 399 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 209/362 (57%), Positives = 266/362 (73%) Query: 16 QEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGKT 75 + QL+ WRRH H PELSF+E T+ +V LREI LE+SRPT SV+ARLIG +PGKT Sbjct: 19 KNQLIEWRRHFHKYPELSFQEEKTSQFVFDILREIPYLEVSRPTKYSVMARLIGRQPGKT 78 Query: 76 LALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIRF 135 +A+RADMDALPIHEEN+F+F S PGVMHACGHDGH +ILLGVV L +R ++ GEIRF Sbjct: 79 IAVRADMDALPIHEENKFDFISIYPGVMHACGHDGHMAILLGVVHKLVEEREKVKGEIRF 138 Query: 136 IFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIVV 195 +FQHAEE DYI+G HL + L +G++GV YG MA+PD F I + Sbjct: 139 LFQHAEENFPGGAEEMVAAGVMTGVDYIIGAHLWASLEVGKVGVIYGPAMAAPDVFKITI 198 Query: 196 KGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPDT 255 +G GGH G PH++VD IAIGAQ+VS +Q I SR TNPLD LVVS+TQF SG++HNVIP+ Sbjct: 199 EGKGGHAGIPHETVDSIAIGAQVVSQIQQIVSRLTNPLDSLVVSVTQFHSGTTHNVIPEQ 258 Query: 256 ATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAEE 315 A ++GTVRS ++R++ +++L RIV+ IT ++GA+Y Y GY PVVND+ +T L E Sbjct: 259 AEVEGTVRSLRHELREETKKKLERIVKHITESYGAKYTFSYEYGYRPVVNDYEVTELIEH 318 Query: 316 AAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDEA 375 A++L GR+ VV+ P M+GEDFSA+ Q+ PGTF IGAGN +KGI+YPHHHPRFT DE Sbjct: 319 TALQLYGRERVVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNEEKGIIYPHHHPRFTIDED 378 Query: 376 AL 377 AL Sbjct: 379 AL 380 >C2YM77.1 C2YM77_BACCE Aminoacylase (N-acyl-L-amino acid amidohydrolase) Length = 399 Score = 437 bits (1125), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/362 (57%), Positives = 265/362 (73%) Query: 16 QEQLVAWRRHLHLNPELSFEEHHTAAYVEKNLREIGGLEISRPTPTSVVARLIGGKPGKT 75 + QL+ WRRH H PELSF+E T+ +V LR+I LE++RPT SV+ARLIG KPGKT Sbjct: 19 KNQLIEWRRHFHKYPELSFQEEKTSQFVFDILRKITCLEVTRPTKYSVMARLIGKKPGKT 78 Query: 76 LALRADMDALPIHEENEFEFASAVPGVMHACGHDGHTSILLGVVSLLAGQRGQLHGEIRF 135 +A+RADMDALPIHEENEF+F S PGVMHACGHDGH +ILLGVV L R ++ GEIRF Sbjct: 79 IAVRADMDALPIHEENEFDFISTEPGVMHACGHDGHIAILLGVVHKLVEAREKVEGEIRF 138 Query: 136 IFQHAEEXXXXXXXXXXXXXXXXXXDYIVGEHLASQLPLGQIGVAYGEMMASPDNFTIVV 195 +FQHAEE DYI+G HL + L +G+IGV YG MA+PD F I + Sbjct: 139 LFQHAEENFPGGAEEMVAAGVMEGVDYIIGAHLWASLQVGKIGVIYGPAMAAPDVFKITI 198 Query: 196 KGYGGHGGFPHKSVDPIAIGAQIVSNLQHIASRNTNPLDRLVVSITQFTSGSSHNVIPDT 255 +G GGH G PH++VD IAIG Q+VS LQ I SR TNPLD LVVS+TQF +G++HNVIP+ Sbjct: 199 EGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHAGTTHNVIPEQ 258 Query: 256 ATLKGTVRSFDPQVRKDAQQQLRRIVEGITNAHGAEYELDYLLGYDPVVNDHTLTRLAEE 315 A ++GTVRS ++R++ ++++ RIV+ +T A+GA+Y Y GY PVVND+ +T + E+ Sbjct: 259 AKIEGTVRSLKHELREETEKRIERIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEVIEQ 318 Query: 316 AAVELVGRDNVVQTPPGMSGEDFSAYQQRVPGTFINIGAGNPDKGILYPHHHPRFTFDEA 375 A++L GR+ V + P M+GEDFSA+ Q+VPGTF IGAGN +KGI+YPHHHPRFT DE Sbjct: 319 TALQLYGREGVTRLQPTMAGEDFSAFLQKVPGTFFFIGAGNKEKGIIYPHHHPRFTIDED 378 Query: 376 AL 377 AL Sbjct: 379 AL 380 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,493,160,468 Number of extensions: 103537027 Number of successful extensions: 255565 Number of sequences better than 1.0e-05: 2710 Number of HSP's gapped: 247662 Number of HSP's successfully gapped: 2734 Length of query: 410 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 272 Effective length of database: 1,760,596,133 Effective search space: 478882148176 Effective search space used: 478882148176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)