BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0043.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4IU28.1 A4IU28_GEOTN Alkane monoxygenase 275 2e-72 A8DC15.1 A8DC15_BACST Alkane monooxygenase 275 3e-72 B2TDZ4.1 B2TDZ4_BURPP Flavin-dependent oxidoreductase 270 1e-70 B1G9Y6.1 B1G9Y6_9BURK Flavin-dependent oxidoreductase 270 1e-70 Q9I1D0.1 Q9I1D0_PSEAE Putative uncharacterized protein 258 6e-67 >A4IU28.1 A4IU28_GEOTN Alkane monoxygenase Length = 440 Score = 275 bits (704), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 141/292 (48%), Positives = 182/292 (62%), Gaps = 19/292 (6%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 +EV Y+L EGSW+D+AV D E+ Y D KVH + H GKY+E+ H CEPS QRTPV+ Sbjct: 165 LEVCYKLWEGSWEDNAVIRDIENNIYTDPSKVHEINHSGKYFEVPGPHLCEPSPQRTPVI 224 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 +QAG SERGR FAAKHAE VFL VE L+ V DIR RA YGR P+ IK+F G+ + Sbjct: 225 YQAGMSERGREFAAKHAECVFLGGKDVETLKFFVDDIRKRAKKYGRNPDHIKMFAGICVI 284 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 VG+T EA++KL S+++Y S E L Y TG DL+ + +IG Sbjct: 285 VGKTHDEAMEKLNSFQKYWSLEGHLAHYGGGTGYDLSKYSSN-DYIG------------- 330 Query: 181 TKHSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPGN 240 S SV + M GK F+ GT ++AD+MQY VEE+GIDGFNL +Y PG Sbjct: 331 ---SISVGEIINNMSKLDGKWFK--LSVGTPKKVADEMQYLVEEAGIDGFNLVQYVSPGT 385 Query: 241 HREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFRQVT 292 +F++ VVPELQ RG++R YEE T RE+LFG+G RLPD H A +R ++ Sbjct: 386 FVDFIELVVPELQKRGLYRVDYEEGTYREKLFGKGNYRLPDDHIAARYRNIS 437 >A8DC15.1 A8DC15_BACST Alkane monooxygenase Length = 440 Score = 275 bits (703), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 141/292 (48%), Positives = 182/292 (62%), Gaps = 19/292 (6%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 +EV Y+L EGSW+D+AV D E+ Y D KVH + H GKY+E+ H CEPS QRTPV+ Sbjct: 165 LEVCYKLWEGSWEDNAVIRDIENNIYTDPSKVHEINHSGKYFEVPGPHLCEPSPQRTPVI 224 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 +QAG SERGR FAAKHAE VFL VE L+ V DIR RA YGR P+ IK+F G+ + Sbjct: 225 YQAGMSERGREFAAKHAETVFLGGTDVETLKFFVDDIRKRAKKYGRNPDHIKMFAGICVI 284 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 VG+T EA++KL S+++Y S E L Y TG DL+ + +IG Sbjct: 285 VGKTHDEAMEKLNSFQKYWSLEGHLAHYGGGTGYDLSKYSSN-DYIG------------- 330 Query: 181 TKHSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPGN 240 S SV + M GK F+ GT ++AD+MQY VEE+GIDGFNL +Y PG Sbjct: 331 ---SISVGEIINNMSKLDGKWFK--LSVGTPKKVADEMQYLVEEAGIDGFNLVQYVSPGT 385 Query: 241 HREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFRQVT 292 +F++ VVPELQ RG++R YEE T RE+LFG+G RLPD H A +R ++ Sbjct: 386 FVDFIELVVPELQKRGLYRVDYEEGTYREKLFGKGNYRLPDDHIAARYRNIS 437 >B2TDZ4.1 B2TDZ4_BURPP Flavin-dependent oxidoreductase Length = 468 Score = 270 bits (690), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 141/292 (48%), Positives = 192/292 (65%), Gaps = 3/292 (1%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 M+V Y+L E SW+DDAV D+ R ++ DKVH VKH G YY + A H EPS QRTPVL Sbjct: 166 MDVVYKLWEQSWEDDAVVRDRAARIFSHPDKVHRVKHDGPYYSIDAIHLSEPSPQRTPVL 225 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 +QAGSS RG FAA+HAE VF+ + R+ V DIR+RA ++GR P+ IKIF G++ V Sbjct: 226 YQAGSSSRGVDFAARHAECVFVGGQNKQLTRSIVDDIRARAVSFGRAPDDIKIFAGITVV 285 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 VG+T + A +K E YRRYAS E + + +STGID + + D P V+TE ++ E Sbjct: 286 VGETERLAQEKFEEYRRYASAEGGIAHFSSSTGIDFSQYELDEPISYVKTESMQSAVEAI 345 Query: 181 TKHSGS-VQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPG 239 +K S S V T R+V++ T R V G+ +IAD++ W+EE+G+DGFNL R +P Sbjct: 346 SKKSVSGVWTKRKVLEQMTLGG-RAKPVVGSPQQIADELVSWIEEAGVDGFNLTRTVMPE 404 Query: 240 NHREFVDYVVPELQARGMFRRHYEET-TLRERLFGQGKQRLPDTHPGAAFRQ 290 + +FV+ VVPELQ RG+++ Y+ TLRE+LFG G+ RLPD H GA R+ Sbjct: 405 SFEDFVNLVVPELQNRGIYKEDYDPAPTLREKLFGGGRARLPDVHAGAQHRR 456 >B1G9Y6.1 B1G9Y6_9BURK Flavin-dependent oxidoreductase Length = 465 Score = 270 bits (690), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 141/292 (48%), Positives = 191/292 (65%), Gaps = 3/292 (1%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 M+V Y+L E SW+DDAV D+ R ++ KVH VKH G YY + A H EPS QRTPVL Sbjct: 166 MDVVYKLWEQSWEDDAVVRDRAARIFSQPGKVHRVKHDGPYYSIDAIHLSEPSPQRTPVL 225 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 +QAGSS RG FAA+HAE VF+ + R+ V DIR+RA ++GR P+ IKIF G++ V Sbjct: 226 YQAGSSSRGVDFAARHAECVFVGGQNKQLTRSIVDDIRARAVSFGRAPDDIKIFAGITVV 285 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 VG+T + A +K E YRRYAS E + + +STGID + + D P V+TE ++ E Sbjct: 286 VGETERAAQEKFEEYRRYASAEGGIAHFSSSTGIDFSQYELDEPISYVKTESMQSAVEAI 345 Query: 181 TKHSGS-VQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPG 239 +K S S V T R+V++ T R V G+ +IAD++ W+EE+G+DGFNL R +P Sbjct: 346 SKKSVSGVWTKRKVLEQMTLGG-RAKPVVGSPQQIADELVSWIEEAGVDGFNLTRTVMPE 404 Query: 240 NHREFVDYVVPELQARGMFRRHYEET-TLRERLFGQGKQRLPDTHPGAAFRQ 290 + +FVD VVPELQ RG+++ Y+ TLRE+LFG G+ RLPD H GA R+ Sbjct: 405 SFEDFVDLVVPELQNRGVYKEDYDPAPTLREKLFGGGRARLPDVHAGAQHRR 456 >Q9I1D0.1 Q9I1D0_PSEAE Putative uncharacterized protein Length = 462 Score = 258 bits (658), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 136/290 (46%), Positives = 185/290 (63%), Gaps = 2/290 (0%) Query: 1 MEVSYRLLEGSWQDDAVSADKEHRYYADADKVHIVKHQGKYYEMTASHACEPSIQRTPVL 60 +EV Y+L EGSW+DDAV AD++ R YA +K+H ++H G++Y++ H EPS QRTPVL Sbjct: 166 LEVLYKLWEGSWEDDAVLADRQRRVYARPEKIHKIRHHGEFYQVEGYHLAEPSPQRTPVL 225 Query: 61 FQAGSSERGRAFAAKHAEGVFLKAPTVEALRAQVQDIRSRAAAYGRLPEQIKIFTGLSAV 120 FQAGSSERG AFAA+HAE VF+ T EA R VQ IR+ GR E I+IF G++ + Sbjct: 226 FQAGSSERGLAFAARHAECVFVSGQTREATRQLVQRIRAAVVGAGRRAEDIRIFMGINVI 285 Query: 121 VGQTAQEALDKLESYRRYASREAALLTYENSTGIDLTAMDPDAPFIGVQTEQGRTHTERY 180 V TA EA DK YRRYAS EA L + +S+GIDL+ D P I + + Sbjct: 286 VAATAAEARDKHAEYRRYASPEAGLAHFASSSGIDLSRYGLDEP-IRYEKNNAIESVVKN 344 Query: 181 TKHSGSVQTVREVMDNFTGKEFRGITVAGTAGEIADQMQYWVEESGIDGFNLERYTLPGN 240 + + TVR+++D R +T+ G ++AD ++ W+ ++G+DGFNL R P + Sbjct: 345 LTVARTDSTVRKLLDQLALGG-RYVTLVGDPRQVADDLEGWIADTGLDGFNLTRTVEPES 403 Query: 241 HREFVDYVVPELQARGMFRRHYEETTLRERLFGQGKQRLPDTHPGAAFRQ 290 + +F+D VVPELQARG ++ Y TLRE+LFG G+ RL H GAAFRQ Sbjct: 404 YADFIDLVVPELQARGSYKTAYAPGTLREKLFGDGRARLQAEHAGAAFRQ 453 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,730,055,099 Number of extensions: 70419580 Number of successful extensions: 178365 Number of sequences better than 1.0e-05: 923 Number of HSP's gapped: 177150 Number of HSP's successfully gapped: 929 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)