BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0042.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O35018.1 LMRB_BACSU Lincomycin resistance protein lmrB 608 e-172 A7Z121.1 A7Z121_BACA2 LmrB 593 e-167 B7HXI6.1 B7HXI6_BACC7 Lincomycin resistance protein LmrB 551 e-155 C2RZK7.1 C2RZK7_BACCE Lincomycin resistance protein lmrB 551 e-155 B5V5M3.1 B5V5M3_BACCE Lincomycin resistance protein LmrB 551 e-155 >O35018.1 LMRB_BACSU Lincomycin resistance protein lmrB Length = 479 Score = 608 bits (1567), Expect = e-172, Method: Compositional matrix adjust. Identities = 305/470 (64%), Positives = 358/470 (76%), Gaps = 1/470 (0%) Query: 13 DRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLTLGILV 72 +++K MPI+ISLLLAGFIGMFSETALN+AL+DLMK L+IT AT QWLTT YLL LGILV Sbjct: 10 SQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILV 69 Query: 73 PISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLPLMFNT 132 P+SGLLLQWF+TRQLF +L FSILGTF+AA++P+F FLL AR+VQA+GT LLLPLMFNT Sbjct: 70 PVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNT 129 Query: 133 ILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFLVIALISGI 192 ILVI P KRGAAMG IGLVIM APAIGPT +GL++E L+WHWIFW+SLPFLV+AL+ GI Sbjct: 130 ILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGI 189 Query: 193 FFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEG-WGSPKXXXXXXXXXXXXX 251 +MQNV+E TKPKID E G W SP Sbjct: 190 AYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLI 249 Query: 252 XXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGYSAFKA 311 RQ+TMKQPM+NLRAFK+PMF++GV+MVFICMMVILSSML+LPMYLQ G +AF + Sbjct: 250 LFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFAS 309 Query: 312 XXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVVFVIVL 371 MSP+ GRLFDKYGPKWLVIPG +IV V LWFFS++T TST V +I+L Sbjct: 310 GLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIIL 369 Query: 372 HSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMTAGSKS 431 H+ LM+G+SMI MPAQTNG+NQLP E YPDGTAIMNTLQQ+AGAIGTA+AVSIM AG Sbjct: 370 HTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHD 429 Query: 432 YMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQH 481 YM +V NP+DPA + A T GVQ+AFVF M +AIIGL+ AFF+KRV V H Sbjct: 430 YMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH 479 >A7Z121.1 A7Z121_BACA2 LmrB Length = 477 Score = 593 bits (1529), Expect = e-167, Method: Compositional matrix adjust. Identities = 293/476 (61%), Positives = 358/476 (75%), Gaps = 1/476 (0%) Query: 8 KKQTEDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAYLLT 67 + +++K MPI+ISLL AGFIGMFSETALN+AL+DLMK L ITPAT QWLTT YLL Sbjct: 2 ENAQPSKQYKVMPIMISLLTAGFIGMFSETALNIALTDLMKELHITPATVQWLTTGYLLV 61 Query: 68 LGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTALLLP 127 LGILVP+SGLLLQWF+TRQLFI +L+FSI+GT +AA++P+F FLL AR+VQA+GT LLLP Sbjct: 62 LGILVPVSGLLLQWFTTRQLFIVSLAFSIIGTLIAALAPSFPFLLAARIVQALGTGLLLP 121 Query: 128 LMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFLVIA 187 LMFNTILVI P KRGAAMG IGLVIM APAIGPT +GL++E L+WHWIFW+SLPFLV+A Sbjct: 122 LMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLA 181 Query: 188 LISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXE-AEGWGSPKXXXXXXXX 246 L+ GI +MQNV+++TKPKID E ++GW SP Sbjct: 182 LLFGIAYMQNVSDITKPKIDVLSIILSTIGFGGIVFGFSNAGEGSDGWSSPTVIGSLTVG 241 Query: 247 XXXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQGY 306 RQ+TMKQPM+NLRAF++PMFV+GV++VFICMMVILS+ML+LPMYLQ G Sbjct: 242 AIALILFSIRQLTMKQPMMNLRAFRYPMFVLGVVIVFICMMVILSTMLLLPMYLQSGLML 301 Query: 307 SAFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATSTVV 366 +AF + MSP+ GRLFDKYGPKWLVIPG +I A +L FFS+I ST + Sbjct: 302 TAFTSGLILLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVITATALSFFSNINGASTAL 361 Query: 367 FVIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSIMT 426 ++ LH+ LM+G+SMI MPAQTNG+NQLP E YPDGTAIMNTLQQ+AGAIGTA+AVSIM Sbjct: 362 LIVALHTCLMIGISMIMMPAQTNGLNQLPPEFYPDGTAIMNTLQQMAGAIGTAVAVSIMA 421 Query: 427 AGSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQHK 482 AG K+YM +V NP+DP+ A GVQ+AF+F M +A+IGL+ AFF+KRV V H Sbjct: 422 AGQKNYMSTVKNPNDPSAYSHALIAGVQHAFIFAMIVAVIGLISAFFMKRVKVNHS 477 >B7HXI6.1 B7HXI6_BACC7 Lincomycin resistance protein LmrB Length = 480 Score = 551 bits (1421), Expect = e-155, Method: Compositional matrix adjust. Identities = 271/479 (56%), Positives = 358/479 (74%), Gaps = 2/479 (0%) Query: 7 GKKQT--EDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAY 64 G +QT E+ + +PI+ S L+AGFIG+FSETALN+AL DL+ DI TAQWLTT Y Sbjct: 2 GNQQTTAENSNIRALPIITSFLIAGFIGLFSETALNMALKDLITGFDILETTAQWLTTGY 61 Query: 65 LLTLGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTAL 124 LLTLGILVPISGL+LQWF+TRQLFIA+L FSI+GT + A++P F L+IAR+VQA+GTAL Sbjct: 62 LLTLGILVPISGLILQWFTTRQLFIASLVFSIIGTLIGAIAPTFSVLMIARIVQAVGTAL 121 Query: 125 LLPLMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFL 184 LLPLMFNTILVIIP KRG AMG+IGLVIM APA+GPT++GL++++L+WHWIFW+SLP L Sbjct: 122 LLPLMFNTILVIIPPHKRGRAMGIIGLVIMFAPAVGPTVSGLILKTLTWHWIFWISLPLL 181 Query: 185 VIALISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXX 244 +IAL+ G FFMQNVT++TKP+ID E+ GWGS Sbjct: 182 IIALVFGTFFMQNVTKLTKPRIDIFSIILSTIGFGGIVFGFSSAGESGGWGSSHVILSLV 241 Query: 245 XXXXXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQ 304 RQM M +PM++L+AFK+PMFV+G+L++ +CMMV+L +M+ILP+YLQ Sbjct: 242 IGVVGLIAFSLRQMKMDEPMLDLKAFKYPMFVIGLLLILVCMMVLLCTMMILPLYLQTSL 301 Query: 305 GYSAFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATST 364 S F A +SP+MG LFD++GPKWLVIPGL+IVA +L+ FS++T ++ Sbjct: 302 ALSTFAAGLMLLPGGIINGILSPVMGGLFDRFGPKWLVIPGLVIVACALYGFSTLTLETS 361 Query: 365 VVFVIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSI 424 F++ LH ALMVG+SMI MPAQTNG+NQLP ELYP GTAIMNTLQQVAGAIGTA+ +SI Sbjct: 362 TGFIVALHIALMVGISMIMMPAQTNGLNQLPPELYPHGTAIMNTLQQVAGAIGTAVGISI 421 Query: 425 MTAGSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQHKA 483 +++GS+S++++VS+ +P N + AFT GVQN F+F + + I+GL+++FF+KRV V+ +A Sbjct: 422 LSSGSRSFLQTVSDKENPLNQVLAFTNGVQNTFIFAIIVVIVGLIISFFIKRVNVEQRA 480 >C2RZK7.1 C2RZK7_BACCE Lincomycin resistance protein lmrB Length = 480 Score = 551 bits (1421), Expect = e-155, Method: Compositional matrix adjust. Identities = 271/479 (56%), Positives = 358/479 (74%), Gaps = 2/479 (0%) Query: 7 GKKQT--EDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAY 64 G +QT E+ + +PI+ S L+AGFIG+FSETALN+AL DL+ DI TAQWLTT Y Sbjct: 2 GNQQTTAENSNIRALPIITSFLIAGFIGLFSETALNMALKDLITGFDILETTAQWLTTGY 61 Query: 65 LLTLGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTAL 124 LLTLGILVPISGL+LQWF+TRQLFIA+L FSI+GT + A++P F L+IAR+VQA+GTAL Sbjct: 62 LLTLGILVPISGLILQWFTTRQLFIASLVFSIIGTLIGAIAPTFSVLMIARIVQAVGTAL 121 Query: 125 LLPLMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFL 184 LLPLMFNTILVIIP KRG AMG+IGLVIM APA+GPT++GL++++L+WHWIFW+SLP L Sbjct: 122 LLPLMFNTILVIIPPHKRGRAMGIIGLVIMFAPAVGPTVSGLILKTLTWHWIFWISLPLL 181 Query: 185 VIALISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXX 244 +IAL+ G FFMQNVT++TKP+ID E+ GWGS Sbjct: 182 IIALVFGTFFMQNVTKLTKPRIDIFSIILSTIGFGGIVFGFSSAGESGGWGSSHVILSLV 241 Query: 245 XXXXXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQ 304 RQM M +PM++L+AFK+PMFV+G+L++ +CMMV+L +M+ILP+YLQ Sbjct: 242 IGVVGLIAFSLRQMKMDEPMLDLKAFKYPMFVIGLLLILVCMMVLLCTMMILPLYLQTSL 301 Query: 305 GYSAFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATST 364 S F A +SP+MG LFD++GPKWLVIPGL+IVA +L+ FS++T ++ Sbjct: 302 ALSTFAAGLMLLPGGIINGILSPVMGGLFDRFGPKWLVIPGLVIVACALYGFSTLTLETS 361 Query: 365 VVFVIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSI 424 F++ LH ALMVG+SMI MPAQTNG+NQLP ELYP GTAIMNTLQQVAGAIGTA+ +SI Sbjct: 362 TGFIVALHIALMVGISMIMMPAQTNGLNQLPPELYPHGTAIMNTLQQVAGAIGTAVGISI 421 Query: 425 MTAGSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQHKA 483 +++GS+S++++VS+ +P N + AFT GVQN F+F + + I+GL+++FF+KRV V+ +A Sbjct: 422 LSSGSRSFLQTVSDKENPLNQVLAFTNGVQNTFIFAIIVVIVGLIISFFIKRVNVEQRA 480 >B5V5M3.1 B5V5M3_BACCE Lincomycin resistance protein LmrB Length = 480 Score = 551 bits (1421), Expect = e-155, Method: Compositional matrix adjust. Identities = 271/479 (56%), Positives = 358/479 (74%), Gaps = 2/479 (0%) Query: 7 GKKQT--EDRKFKTMPILISLLLAGFIGMFSETALNVALSDLMKVLDITPATAQWLTTAY 64 G +QT E+ + +PI+ S L+AGFIG+FSETALN+AL DL+ DI TAQWLTT Y Sbjct: 2 GNQQTTAENSNIRALPIITSFLIAGFIGLFSETALNMALKDLITGFDILETTAQWLTTGY 61 Query: 65 LLTLGILVPISGLLLQWFSTRQLFIAALSFSILGTFLAAMSPNFEFLLIARVVQAMGTAL 124 LLTLGILVPISGL+LQWF+TRQLFIA+L FSI+GT + A++P F L+IAR+VQA+GTAL Sbjct: 62 LLTLGILVPISGLILQWFTTRQLFIASLVFSIIGTLIGAIAPTFSVLMIARIVQAVGTAL 121 Query: 125 LLPLMFNTILVIIPAEKRGAAMGLIGLVIMVAPAIGPTIAGLLIESLSWHWIFWLSLPFL 184 LLPLMFNTILVIIP KRG AMG+IGLVIM APA+GPT++GL++++L+WHWIFW+SLP L Sbjct: 122 LLPLMFNTILVIIPPHKRGRAMGIIGLVIMFAPAVGPTVSGLILKTLTWHWIFWISLPLL 181 Query: 185 VIALISGIFFMQNVTEVTKPKIDXXXXXXXXXXXXXXXXXXXXXXEAEGWGSPKXXXXXX 244 +IAL+ G FFMQNVT++TKP+ID E+ GWGS Sbjct: 182 IIALVFGTFFMQNVTKLTKPRIDIFSIILSTIGFGGIVFGFSSAGESGGWGSSHVILSLV 241 Query: 245 XXXXXXXXXXXRQMTMKQPMINLRAFKFPMFVVGVLMVFICMMVILSSMLILPMYLQQGQ 304 RQM M +PM++L+AFK+PMFV+G+L++ +CMMV+L +M+ILP+YLQ Sbjct: 242 IGVVGLIAFSLRQMKMDEPMLDLKAFKYPMFVIGLLLILVCMMVLLCTMMILPLYLQTSL 301 Query: 305 GYSAFKAXXXXXXXXXXXXXMSPIMGRLFDKYGPKWLVIPGLIIVAVSLWFFSSITATST 364 S F A +SP+MG LFD++GPKWLVIPGL+IVA +L+ FS++T ++ Sbjct: 302 ALSTFAAGLMLLPGGIINGILSPVMGGLFDRFGPKWLVIPGLVIVACALYGFSTLTLETS 361 Query: 365 VVFVIVLHSALMVGVSMIFMPAQTNGINQLPMELYPDGTAIMNTLQQVAGAIGTALAVSI 424 F++ LH ALMVG+SMI MPAQTNG+NQLP ELYP GTAIMNTLQQVAGAIGTA+ +SI Sbjct: 362 TGFIVALHIALMVGISMIMMPAQTNGLNQLPPELYPHGTAIMNTLQQVAGAIGTAVGISI 421 Query: 425 MTAGSKSYMESVSNPSDPANLLPAFTQGVQNAFVFGMAMAIIGLVVAFFLKRVIVQHKA 483 +++GS+S++++VS+ +P N + AFT GVQN F+F + + I+GL+++FF+KRV V+ +A Sbjct: 422 LSSGSRSFLQTVSDKENPLNQVLAFTNGVQNTFIFAIIVVIVGLIISFFIKRVNVEQRA 480 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,304,647,206 Number of extensions: 83500100 Number of successful extensions: 491259 Number of sequences better than 1.0e-05: 9618 Number of HSP's gapped: 486348 Number of HSP's successfully gapped: 10689 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)