BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0041.p (192 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P42105.2 YXAF_BACSU Uncharacterized HTH-type transcriptional reg... 152 2e-35 Q65L49.1 Q65L49_BACLD YxaF 136 8e-31 Q8TU95.1 Q8TU95_METAC Transcriptional regulator, TetR family 133 8e-30 C4C865.1 C4C865_9FUSO Transcriptional regulator, TetR family 130 5e-29 A7Z977.1 A7Z977_BACA2 YxaF 129 1e-28 >P42105.2 YXAF_BACSU Uncharacterized HTH-type transcriptional regulator yxaF Length = 191 Score = 152 bits (383), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 77/188 (40%), Positives = 121/188 (64%), Gaps = 1/188 (0%) Query: 4 LSNKPNAREAIVDTASRLFFTQGYHATGLSQIIKDSDSPKGSLYYYFPHGKEELALTCIH 63 ++++ ++RE I+ TASRLF QGYHATGL+QI+K+S +PKGSLY++FP+GKEELA+ + Sbjct: 1 MTSRGDSREKILHTASRLFQLQGYHATGLNQIVKESGAPKGSLYHFFPNGKEELAIEAVT 60 Query: 64 KTSEIVVRQLKQYVEKDVAVEQAVQDFILQMVRDAEESDYKGMVPFSFWVAVETSCISED 123 T +IV ++Q +++ +A+Q FI + + ++ +P +A ET+ ISE Sbjct: 61 YTGKIVEHLIQQSMDESSDPVEAIQLFIKKTASQFDNTESIKGIPVGL-LASETALISEP 119 Query: 124 LRTACQAVFKDWQDVILRRLLEEGTDAELAADKASVVVSLFEGALLQTLTCRSTGPLLAA 183 LRT C VFK W+ V R+L+E G E A +++ S+ EG ++ +LT + PLL Sbjct: 120 LRTVCMKVFKSWEAVFARKLMENGFAEEEANQLGTLINSMIEGGIMLSLTNKDKTPLLLI 179 Query: 184 AKMIPGLL 191 A+ IP L+ Sbjct: 180 AEQIPVLV 187 >Q65L49.1 Q65L49_BACLD YxaF Length = 192 Score = 136 bits (343), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 84/198 (42%), Positives = 118/198 (59%), Gaps = 19/198 (9%) Query: 4 LSNKPNAREAIVDTASRLFFTQGYHATGLSQIIKDSDSPKGSLYYYFPHGKEELALTCIH 63 ++ K ++R+ I+ TASRLF QGYHATGLSQI +S +P+GSLYYYFP GKEELA I Sbjct: 1 MAEKMSSRDKILRTASRLFRKQGYHATGLSQITAESGAPRGSLYYYFPGGKEELAQEAIQ 60 Query: 64 KTSEIVVRQLKQYVEKDVAVE----QAVQDFILQMVRDA------EESDYKGMVPFSFWV 113 T E ++K Y+ ++ E +A Q +I QM+ D EE+D +V V Sbjct: 61 LTGE----RIKAYISSAISGEADPVKAFQSYI-QMMADGIEEYIIEENDDIPIVS----V 111 Query: 114 AVETSCISEDLRTACQAVFKDWQDVILRRLLEEGTDAELAADKASVVVSLFEGALLQTLT 173 A ET SE LR C+ V++DWQ V +LL+ G E A + + ++ EGA + ++T Sbjct: 112 AAETWSFSERLRQTCEQVYQDWQLVYQEKLLQSGYAPETAETLSVAIQAMIEGAYVLSVT 171 Query: 174 CRSTGPLLAAAKMIPGLL 191 ++ PL AAA+ IP LL Sbjct: 172 KKNGRPLSAAAEQIPFLL 189 >Q8TU95.1 Q8TU95_METAC Transcriptional regulator, TetR family Length = 214 Score = 133 bits (334), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 74/185 (40%), Positives = 110/185 (59%), Gaps = 1/185 (0%) Query: 7 KPNAREAIVDTASRLFFTQGYHATGLSQIIKDSDSPKGSLYYYFPHGKEELALTCIHKTS 66 K N RE I+ TA+ LF +GYHATGL++IIK+S +PKGSLYYYFP+GKEELA I Sbjct: 28 KMNTREKILTTAAFLFQKKGYHATGLNEIIKESATPKGSLYYYFPNGKEELAEATIKLMG 87 Query: 67 EIVVRQLKQYVEKDVAVEQAVQDFILQMVRDAEESDYKGMVPFSFWVAVETSCISEDLRT 126 + + +++ + K+ QA+++ IL + R E D + +A+ET ISE LR Sbjct: 88 DKIQHNIEEELAKNNDPVQAIEELILDLARKFNEKDQENCFSIGL-LALETVSISESLRK 146 Query: 127 ACQAVFKDWQDVILRRLLEEGTDAELAADKASVVVSLFEGALLQTLTCRSTGPLLAAAKM 186 AC V+ W D ++L+ G +E +A+ + ++ EGA+ +L L+AAA+ Sbjct: 147 ACIEVYDLWTDTYYQKLVSSGFSSEKSAELSLILQLTIEGAITISLIRNDNTMLIAAAEK 206 Query: 187 IPGLL 191 IP LL Sbjct: 207 IPILL 211 >C4C865.1 C4C865_9FUSO Transcriptional regulator, TetR family Length = 192 Score = 130 bits (327), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 77/192 (40%), Positives = 111/192 (57%), Gaps = 13/192 (6%) Query: 6 NKPNAREAIVDTASRLFFTQGYHATGLSQIIKDSDSPKGSLYYYFPHGKEELALTCI--- 62 NK +R+ I++TA LF +QGYHATGL+QIIK+S +PKGS+Y+YFP GKEELA+ I Sbjct: 4 NKGKSRQKILETAGELFKSQGYHATGLNQIIKESGAPKGSIYHYFPGGKEELAIESIKLA 63 Query: 63 -HKTSEIVVRQLKQYVEKDVAVEQAVQDFILQMVRD--AEESDYKGMVPFSFWVAVETSC 119 K E ++ L QY E ++E+ +L+ + D + Y G+ +A+ETS Sbjct: 64 VEKVKEGLIDNLSQYSEPLKSIEE-----LLKKMADEFNPHNPYHGLS--INMLAMETSL 116 Query: 120 ISEDLRTACQAVFKDWQDVILRRLLEEGTDAELAADKASVVVSLFEGALLQTLTCRSTGP 179 ISE LR AC FK W+++ +L+E G E A D + L EGA+ +L + P Sbjct: 117 ISEPLRCACLEAFKTWENIFYDKLIEGGYSPEDAEDLGMTIHFLVEGAVALSLVRQDNKP 176 Query: 180 LLAAAKMIPGLL 191 L ++ I LL Sbjct: 177 FLIVSRQIRKLL 188 >A7Z977.1 A7Z977_BACA2 YxaF Length = 191 Score = 129 bits (325), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 68/185 (36%), Positives = 106/185 (57%), Gaps = 1/185 (0%) Query: 4 LSNKPNAREAIVDTASRLFFTQGYHATGLSQIIKDSDSPKGSLYYYFPHGKEELALTCIH 63 +++K +E I+ ++ LF QGYHATGL+QIIK+S +P+GS+Y++FP+GK ELA+ I Sbjct: 1 MNSKTGTKERIIQASAYLFQIQGYHATGLNQIIKESGAPRGSVYHHFPNGKAELAIAAIK 60 Query: 64 KTSEIVVRQLKQYVEKDVAVEQAVQDFILQMVRDAEESDYKGMVPFSFWVAVETSCISED 123 T V +Q++ + + +A+Q FI + VP +A ET+ I+E Sbjct: 61 YTGRCVEKQIQNSMSQFADPVEAIQHFIHVTAEQFNDPQNIEGVPVGL-LAGETALINET 119 Query: 124 LRTACQAVFKDWQDVILRRLLEEGTDAELAADKASVVVSLFEGALLQTLTCRSTGPLLAA 183 LR C VFK W DV + LE G + E A + S+ EGA++ +L ++ PLL Sbjct: 120 LRCTCVEVFKKWTDVFAAKFLEHGFEQEEAEKLGVTINSMIEGAIMFSLAHKNKEPLLLI 179 Query: 184 AKMIP 188 +K +P Sbjct: 180 SKQVP 184 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,051,743,798 Number of extensions: 38638720 Number of successful extensions: 117709 Number of sequences better than 1.0e-05: 229 Number of HSP's gapped: 118131 Number of HSP's successfully gapped: 229 Length of query: 192 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 64 Effective length of database: 1,854,806,283 Effective search space: 118707602112 Effective search space used: 118707602112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)