BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0036.p (249 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q1AZB9.1 Q1AZB9_RUBXD Putative uncharacterized protein 243 8e-63 Q986I4.1 Q986I4_RHILO Mll7342 protein 213 1e-53 Q0RGJ2.1 Q0RGJ2_FRAAA Putative uncharacterized protein 208 3e-52 Q98IS0.1 Q98IS0_RHILO Mll2277 protein 201 7e-50 B5I996.1 B5I996_9ACTO Putative uncharacterized protein 200 8e-50 >Q1AZB9.1 Q1AZB9_RUBXD Putative uncharacterized protein Length = 274 Score = 243 bits (621), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 116/221 (52%), Positives = 155/221 (70%), Gaps = 1/221 (0%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 GG+ +ADS+ + ++ ETG LPVYYFP +DV L + ++ P KG +W + +GGR Sbjct: 43 GGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAVYWTVRAGGR 102 Query: 67 TARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASRHV 126 TA NA W Y P A L G+++F W +MD WFEE+EEV VH RDPY R+DA+P+SRHV Sbjct: 103 TAENAAWAYPEPLEGAPPLGGHIAFYWDRMDRWFEEDEEVDVHPRDPYHRIDALPSSRHV 162 Query: 127 QLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWTAA 186 ++ VNG +VA++RRPVIL+ETGL PRYY+P EDVR +LL + +S+ CPYKG+A+YW+ Sbjct: 163 RVTVNGELVAETRRPVILFETGLPPRYYIPREDVREELLVPSESSSVCPYKGVASYWSVR 222 Query: 187 VNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 G EDLVWSY EP + + GLLCF++E D L VDG Sbjct: 223 AGGETVEDLVWSYPEPRRDAERVGGLLCFFNERVD-LEVDG 262 Score = 99.0 bits (245), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 47/103 (45%), Positives = 64/103 (62%) Query: 122 ASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIAT 181 + R V++E+ G VADSRR +L+ETGL P YY P EDVR +LL+ ++TRCP+KG A Sbjct: 34 SPRRVRVELGGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAV 93 Query: 182 YWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALY 224 YWT GR E+ W+Y EPL + G + FY + D + Sbjct: 94 YWTVRAGGRTAENAAWAYPEPLEGAPPLGGHIAFYWDRMDRWF 136 >Q986I4.1 Q986I4_RHILO Mll7342 protein Length = 247 Score = 213 bits (543), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 102/230 (44%), Positives = 140/230 (60%) Query: 2 LXXXXGGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDI 61 L G +AD+ + +V+ E+ LPVYYFP+ DV PS P KG+ T + + Sbjct: 7 LRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGVATHYSL 66 Query: 62 TSGGRTARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIP 121 +G +A W Y P + L GY+SF W KM W+EE+EE+FVHARDP+ RVD +P Sbjct: 67 NTGITLVEDAAWRYLDPVKGSPDLSGYMSFYWPKMTHWYEEDEEIFVHARDPFRRVDCLP 126 Query: 122 ASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIAT 181 +SR VQ+ ++G VADSRR V L+ETG R+YLP D RLD+ + +RCPYKGI+ Sbjct: 127 SSRRVQVILDGEQVADSRRGVFLFETGHPVRHYLPISDTRLDMFAPSRYISRCPYKGISN 186 Query: 182 YWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDGIKWS 231 Y+ + +E+LVW Y EP+ E I GL+CF+HE D + VDG+ S Sbjct: 187 YYHVTTPQKRHENLVWYYPEPVHEAERIKGLVCFHHELVDKILVDGVDIS 236 Score = 71.2 bits (173), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 31/97 (31%), Positives = 57/97 (58%) Query: 120 IPASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGI 179 +P + ++++V +IVAD+ + ++LYE+ P YY P DVR + L + +T P+KG+ Sbjct: 1 MPMQKRLRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGV 60 Query: 180 ATYWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFY 216 AT+++ ED W Y +P+ +++G + FY Sbjct: 61 ATHYSLNTGITLVEDAAWRYLDPVKGSPDLSGYMSFY 97 >Q0RGJ2.1 Q0RGJ2_FRAAA Putative uncharacterized protein Length = 245 Score = 208 bits (530), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 102/222 (45%), Positives = 139/222 (62%), Gaps = 4/222 (1%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 G + D+ +V E P YY P DV A L P+ + P +G ++D+ GR Sbjct: 24 AGRLVVDTTSPALVWENPHYPAYYLPRADVVAELV-PTARTEHSPSRGEAVYYDVVVEGR 82 Query: 67 TARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASRHV 126 TA A W Y PQ L V W MD W EE+E V+VH R PYTR+DA+P+SRHV Sbjct: 83 TAPAAAWAY--PQSPLEGLRDLVRLDWEAMDRWLEEDEPVYVHPRSPYTRIDALPSSRHV 140 Query: 127 QLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWTAA 186 ++E++G++VA+S RPV+L+ETGL PRYYLP DVR +LL+ + T CPYKG A Y++ Sbjct: 141 RVEIDGVVVAESHRPVVLFETGLVPRYYLPLVDVRQELLRPSDTRTHCPYKGSAEYFSVE 200 Query: 187 VNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDGI 228 V+GR ++D+VW+Y+ PLPE I GL+CFY E + VDG+ Sbjct: 201 VDGRRHDDVVWTYRTPLPESARITGLVCFYDERV-TVSVDGV 241 >Q98IS0.1 Q98IS0_RHILO Mll2277 protein Length = 257 Score = 201 bits (510), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 106/224 (47%), Positives = 153/224 (68%), Gaps = 4/224 (1%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 GG IADS+ V+V+ LP+Y+FP V+A++ +PS H G +WDI SG R Sbjct: 32 GGRTIADSRNVLVLRSNHFLPIYFFPPSSVDASVLKPSLHREPHAVGGETAYWDIDSGDR 91 Query: 67 TARNAVWTYSGPQPEAHF--LHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASR 124 A NA W+++ P P+ + L G ++F+W +D WFEEEEEVFVHARDPY R+D + +S Sbjct: 92 RAANAAWSFTAP-PDENLSPLAGRIAFTWKAVDQWFEEEEEVFVHARDPYARIDVLHSSS 150 Query: 125 HVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWT 184 HVQ+ +G I+ADS RPV+L+ET L R+YL EDVRL+ L + ++TRCPYKGIA+YW+ Sbjct: 151 HVQVWFDGDIIADSHRPVLLFETHLPTRFYLRPEDVRLERLTASSSTTRCPYKGIASYWS 210 Query: 185 AAV-NGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 + +G D+ WSY++P+ E+ +I GL+ FY +A D +++DG Sbjct: 211 GLLKDGSVRPDIAWSYRDPIDEMPKIKGLIAFYPQAVDRIHLDG 254 >B5I996.1 B5I996_9ACTO Putative uncharacterized protein Length = 262 Score = 200 bits (509), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 104/227 (45%), Positives = 146/227 (64%), Gaps = 16/227 (7%) Query: 7 GGDYIADSKEVMVVTETG-RLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGG 65 G + DS+ ++V E +P+Y FP DV L +P++ AT G Q F+D+ G Sbjct: 31 GDVTVVDSRRPVLVWEPDVPVPLYAFPRADVREDLLRPAKNPATGTHTGSQVFYDLEVDG 90 Query: 66 RTARNAVWTYSGPQPEAHFLHGYVSFSW-----SKMDGWFEEEEEVFVHARDPYTRVDAI 120 NA WT+ A L +++F+W + +D W+EEEEE+FVH RDP+ RVDA+ Sbjct: 91 ELVENAAWTFP-----AADLADHIAFAWFRRWGTGLDHWYEEEEEIFVHPRDPHKRVDAM 145 Query: 121 PASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIA 180 P+SRHVQ+E++G +VAD+RRPV+L+ETGL RYY+P EDVRLDLL + T CPYKG A Sbjct: 146 PSSRHVQVEIDGTVVADTRRPVLLFETGLPTRYYIPREDVRLDLLDATDHHTACPYKGTA 205 Query: 181 TYWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 YW+ ++ ++VWSY +PLP V + GLL F++EA D + VDG Sbjct: 206 GYWSVG----DHANIVWSYPDPLPAVGAVKGLLAFFNEAVD-ITVDG 247 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,645,222,300 Number of extensions: 70989373 Number of successful extensions: 127288 Number of sequences better than 1.0e-05: 353 Number of HSP's gapped: 127172 Number of HSP's successfully gapped: 483 Length of query: 249 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 117 Effective length of database: 1,817,122,223 Effective search space: 212603300091 Effective search space used: 212603300091 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)