BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0035.p (531 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q120I2.1 Q120I2_POLSJ Phenylalanine/histidine ammonia-lyase 373 e-101 C4ESZ6.1 C4ESZ6_9BACT Histidine ammonia-lyase 358 7e-97 Q098W6.1 Q098W6_STIAU Phenylalanine and histidine ammonia-lyase 353 2e-95 Q930N0.1 Q930N0_RHIME Histidine ammonia-lyase EC=4.3.1.3 348 9e-94 Q5M3R0.1 Q5M3R0_STRT2 Histidine ammonia-lyase 334 2e-89 >Q120I2.1 Q120I2_POLSJ Phenylalanine/histidine ammonia-lyase Length = 539 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 220/519 (42%), Positives = 293/519 (56%), Gaps = 35/519 (6%) Query: 24 NPLVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVG 83 P+ L G LTI V V+R R V + + +W R+ + + + A G PVYGLNRGVG Sbjct: 8 EPIELTGRALTIDQVLQVSREDRKVAVSNESWARVARSHALLLSYARLGQPVYGLNRGVG 67 Query: 84 WNKDLE------ISPD---FFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGA 134 NKD I+P+ E +NRN+LLSH+ P DVVRA ML RLN L G Sbjct: 68 LNKDQTPFSGDVIAPEVRALSETFNRNMLLSHTVAYGPLVPADVVRAAMLIRLNTALFGG 127 Query: 135 AGVQPELAGRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPAL 194 G+QPE+ +YA+FLN GI PV+ +GSVGAADIT + I LA++GEGE G R+ + Sbjct: 128 TGMQPEVVRQYAEFLNQGITPVIRGQGSVGAADITVLPQIALALMGEGEVFYRGQRMASA 187 Query: 195 EALKAAGLAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGN 254 E L+ AGL ++ KD L+++ D + LL AD + ALSLE + GN Sbjct: 188 EVLRTAGLGAIKPFAKDSLSMLSANAYGAALAAVAASDAQALLNQADVVAALSLEGLDGN 247 Query: 255 VSPLDEAVHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARD 314 V+P +RPF GQ + + S L GS LW LQDPLSFR QIHGAARD Sbjct: 248 VAPFLAVTQAQRPFFGQSAAAHHIVSLLQGSSLWQRSEQRPLQDPLSFRSTAQIHGAARD 307 Query: 315 AHDFTSRQLVLHLNSSDDNPCVLSD----------EQR-----------IVSCANYDPLS 353 A QLV+ +NSSDDNP V D E+R I+ A++DP Sbjct: 308 ALAALHAQLVVQINSSDDNPTVALDVRPATNATPQERRYFVTEGAVQGAILPSASFDPTV 367 Query: 354 WTLGFEMLGSALHHVSKSSCHRIIKLGDPRFTGLTRFLTADTSRSVGFCTVQKTATSLDA 413 W + E +G A+ HV++SS R+++LGDP FT L+RFL+ + + T+QKT +SL Sbjct: 368 WAVSLEGVGIAMSHVAQSSAQRVLRLGDPAFTRLSRFLSPGGG-VLAYTTIQKTVSSLST 426 Query: 414 EIRHLSNPVSADYLSLAGEIEDHAANSPYVVSKTREILDRLTYILAIELLHAAQAVDLR- 472 E+R LS PVS+D L LAG IED A+N+P + + + + +L IE +HAAQA+DLR Sbjct: 427 EVRALSQPVSSDSLVLAGNIEDVASNAPLAAQRVAQQIKLVRTLLGIETIHAAQAIDLRL 486 Query: 473 ---GGLTLGHASWAGYKSLRRAVPFLSEDRNQSEDIERA 508 TLG + A +K+ R VPFL DR DIE+A Sbjct: 487 QAEPTRTLGAGTRAFHKAFRAQVPFLDADRRLGTDIEKA 525 >C4ESZ6.1 C4ESZ6_9BACT Histidine ammonia-lyase Length = 507 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 209/488 (42%), Positives = 277/488 (56%), Gaps = 6/488 (1%) Query: 33 LTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLP-VYGLNRGVGWNKDLEIS 91 ++I V SVARG V I A QR A + +A++G P VYGLN GVG NKD E++ Sbjct: 10 ISIHQVLSVARGE-GVEIHPEALQRCTATWEALMDIANRGHPKVYGLNTGVGINKDQEVT 68 Query: 92 PDFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAAGVQPELAGRYADFLNL 151 D + YN N+L H G+ P + + RAVM+ +LN LL G G+ P D +NL Sbjct: 69 KDSYCRYNMNMLQVHCVGLPPYGNLEEARAVMVIKLNHLLMGYTGITPRFITLLKDMINL 128 Query: 152 GIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALEALKAAGLAPLRLGPKD 211 G+HP +PLRGSVG ADI +SH+GL M G+G DG VP+ L+ GL ++ GPKD Sbjct: 129 GVHPAIPLRGSVGEADIACLSHLGLVMTGQGLVIQDGRPVPSGPELERMGLEAVQPGPKD 188 Query: 212 GLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNVSPLDEAVHRKRPFPGQ 271 GL +V + ++++TAD +YA++LEA GN SPLD P PG Sbjct: 189 GLGLVTSNALGEALGALILDRIGRIVDTADLVYAMTLEAFHGNPSPLDPRAMDLYPSPGA 248 Query: 272 LKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDAHDFTSRQLVLHLNSSD 331 +S VRS LAGS +W D P+SLQDPLS R + +HG RD + L H+N+ D Sbjct: 249 QRSAQTVRSLLAGSYIWDD--PKSLQDPLSLRCSAMVHGTVRDLIRRCIKVLTAHINAPD 306 Query: 332 DNPCVLSDEQRIVSCANYDPLSWTLGFEMLGSALHHVSKSSCHRIIKLGDPRFTGLTRFL 391 DNP D +V A ++PL W + FE L AL H++++ R ++LG RFTGL RFL Sbjct: 307 DNPLAFPDGT-VVPSALFEPLPWVVHFEGLKVALGHLAQTVARRTLRLGSDRFTGLPRFL 365 Query: 392 TADTSRSVGFCTVQKTATSLDAEIRHLSNPVSADYLSLAGEIEDHAANSPYVVSKTREIL 451 D F +QKT + L AE+RHL NP S D SL+ + ED N+P V+ + +I Sbjct: 366 NNDPCNCHAFGVIQKTLSYLLAEVRHLCNPCSMDVESLSEDQEDRGCNTPMVMDQLGQIA 425 Query: 452 DRLTYILAIELLHAAQAVDLRGGLTLGHASWAGYKSLRRAVPFLSEDRNQSEDIERAYRL 511 RL +LAIE +HAAQ +DLRG G S A + LRR V FL DR+ S DI A L Sbjct: 426 QRLEALLAIEAIHAAQGMDLRGA-RYGVGSSAALERLRREVAFLDVDRDLSCDILSAQAL 484 Query: 512 LKDGTWLN 519 + G L Sbjct: 485 IAGGGLLK 492 >Q098W6.1 Q098W6_STIAU Phenylalanine and histidine ammonia-lyase Length = 546 Score = 353 bits (906), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 212/523 (40%), Positives = 292/523 (55%), Gaps = 37/523 (7%) Query: 26 LVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVGWN 85 + LDG + T + ++A+G+ V I A++R+E A R + A +G +YGL GVG N Sbjct: 24 VTLDGKSATPEAIAAIAQGQ-PVKIAPEAFKRIEQAHRVLLQAAAEGQTIYGLTVGVGLN 82 Query: 86 KDL-------EISPDFFEAYNR---NLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAA 135 KD +++ + EA R L+ +HS V PE D VRA M ARLN +L G A Sbjct: 83 KDRAMVDAQGKLTEEVIEASRRFQVGLIRAHSGSVGPEMDVRTVRATMAARLNTMLVGGA 142 Query: 136 GVQPELAGRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALE 195 GVQPE+ Y FLN GI PV+P GS+G ADIT ISH+GLAM+GEGE + G + A + Sbjct: 143 GVQPEVIDAYVQFLNRGIAPVIPTGGSLGEADITIISHVGLAMLGEGEVYYKGQKTSAAK 202 Query: 196 ALKAAGLAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNV 255 AL+AAG+ P++L KD LA++ D+ L+ +YALSL+AI GNV Sbjct: 203 ALQAAGVKPVKLFGKDALAILSSNAYSAGLAALALNDLSHLITVNKLVYALSLQAINGNV 262 Query: 256 SPLDEAVHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDA 315 SP E RPFP +++ +R L+GS LW LQDPLSFRD+ + G Sbjct: 263 SPFLEDTLALRPFPQTVRAGAELRELLSGSSLWNQDATRRLQDPLSFRDSVYVLGEVTRT 322 Query: 316 HDFTSRQLVLHLNSSDDNPCVLSD----EQR---------------IVSCANYDPLSWTL 356 +D L + LNSSDDNP V + QR ++ AN++PL W L Sbjct: 323 YDEARGLLNIQLNSSDDNPGVAVNVTPKSQRWQEKKSYVAADTAGAVLPSANFEPLPWVL 382 Query: 357 GFEMLGSALHHVSKSSCHRIIKLGDPRFTGLTRFLTADTSRSV-GFCTVQKTATSLDAEI 415 FE LG AL H S +S RIIKL DP TGL R+L T ++V F ++K +L E Sbjct: 383 AFEQLGIALAHNSLTSAQRIIKLDDPNITGLERYL--GTEKTVHAFGAMEKPVVALALEN 440 Query: 416 RHLSNPVSADYLSLAGEIEDHAANSPYVVSKTREILDRLTYILAIELLHAAQAVDLRGG- 474 + L+ PVS ++L +AG IED A N+P VV + ++ +D +L IE +H+AQA+DLR Sbjct: 441 KVLATPVSTEFLPVAGGIEDIATNAPEVVKRVQKQIDNSYALLGIESIHSAQAIDLRRAS 500 Query: 475 ---LTLGHASWAGYKSLRRAVPFLSEDRNQSEDIERAYRLLKD 514 TL A+ Y ++RR V FL EDR + D A +L++ Sbjct: 501 RKEFTLAPATTKLYGAVRRKVAFLDEDRPLTPDFRAAAEVLRN 543 >Q930N0.1 Q930N0_RHIME Histidine ammonia-lyase EC=4.3.1.3 Length = 546 Score = 348 bits (892), Expect = 9e-94, Method: Compositional matrix adjust. Identities = 212/524 (40%), Positives = 290/524 (55%), Gaps = 32/524 (6%) Query: 26 LVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVGWN 85 ++L+G N T + VA+G AV +D + ++E A + A +G +YGL GVGWN Sbjct: 25 VLLNGRNATPEMIVRVAKGE-AVTVDGESLDKVERAYAVLLQGAKEGQEIYGLTVGVGWN 83 Query: 86 KDL-------EISPDFFEA---YNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAA 135 KD E++P+ EA +N LL +HS GV P+A VVRA M RLN +LTG Sbjct: 84 KDRKMVDATGELTPELMEASREFNEGLLRAHSVGVGPDAAVPVVRAAMAVRLNNILTGGP 143 Query: 136 GVQPELAGRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALE 195 GVQP +A FLN GI P +P RGSVG AD+T +SHIGLAM+GEG+ G R+ A E Sbjct: 144 GVQPHVAEMLLAFLNKGITPTMPSRGSVGQADMTLLSHIGLAMLGEGDVDYQGRRMSAAE 203 Query: 196 ALKAAGLAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNV 255 ALKAAG+ PL+ KDGLA++ +D E+LL T+ +YALSLE + GNV Sbjct: 204 ALKAAGIEPLKPFGKDGLAILSSNAYAAGLAALAIHDAEQLLSTSRLVYALSLEGLNGNV 263 Query: 256 SPLDEAVHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDA 315 SPL E V RPFP L S +R+ L GS LW + LQDPLSFR A + G+ D+ Sbjct: 264 SPLLEDVAALRPFPSHLSSTTELRALLEGSYLWQADDKRILQDPLSFRTAPYLLGSFADS 323 Query: 316 HDFTSRQLVLHLNSSDDNPCV---------LSDEQR-------IVSCANYDPLSWTLGFE 359 T + + +NSSDDNP + L +R ++ AN++PL W + FE Sbjct: 324 LARTKALVQIQINSSDDNPGIVVGVEPKSDLFQARRGYVEGGAVLPTANFEPLPWIIAFE 383 Query: 360 MLGSALHHVSKSSCHRIIKLGDPRFTGLTRFLTADTSRSVGFCTVQKTATSLDAEIRHLS 419 LG L H + +S R++KL +P T L R+L + + F V+ +L E R L+ Sbjct: 384 ELGIVLAHHTTASSERVLKLNNPTHTKLARYLGTENTHH-AFLVVEAPLMALATENRALA 442 Query: 420 NPVSADYLSLAGEIEDHAANSPYVVSKTREILDRLTYILAIELLHAAQAVDLR----GGL 475 P S D +AG +ED N+P VV + R +D IL++E+LHAAQA DLR Sbjct: 443 QPTSFDSRPIAGGVEDVGTNAPLVVERIRAQIDNSFTILSMEMLHAAQAADLRLKGHPER 502 Query: 476 TLGHASWAGYKSLRRAVPFLSEDRNQSEDIERAYRLLKDGTWLN 519 L A+ + + + R+ VPFL DR+ + DI LK+ N Sbjct: 503 KLSTATTSFHDAFRQRVPFLDRDRSMTPDIAAGTAFLKEYALTN 546 >Q5M3R0.1 Q5M3R0_STRT2 Histidine ammonia-lyase Length = 498 Score = 334 bits (856), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 192/445 (43%), Positives = 266/445 (59%), Gaps = 2/445 (0%) Query: 28 LDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVGWNKD 87 L G LT+ ++ V +G + V I A+ R++A+R+ + V D +YGLN GVG NKD Sbjct: 3 LTGYGLTVESIERVVKGEK-VTIAPEAYHRLKASRKIVLQVLDSQQQIYGLNTGVGKNKD 61 Query: 88 LEISPDFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAAGVQPELAGRYAD 147 +I D E +NR L+ +H G++PE D VRA ML LN +L G AG+Q + R D Sbjct: 62 RKIPKDKIEEFNRQLIYAHCVGIEPELSIDNVRAAMLIHLNNMLKGYAGIQVAIPERLVD 121 Query: 148 FLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALEALKAAGLAPLRL 207 FLNLG+ PV+ GSVG DI +S+IGL ++GEG +G+R A E K L P+ Sbjct: 122 FLNLGVTPVVNGFGSVGEGDIGILSYIGLGLMGEGLVDYEGNRYEAREVFKRLHLKPIHF 181 Query: 208 GPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNVSPLDEAVHRKRP 267 KDGLAV+ ++ LL D +LSLEA+ GN++PLD+ V + + Sbjct: 182 YAKDGLAVISSNAVSFSRLSLLIPKIKNLLYWFDLACSLSLEALGGNITPLDDLVVKAKH 241 Query: 268 FPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDAHDFTSRQLVLHL 327 GQ S +S+L S L + +++QDPLSFR+A IHGAA D+ + QL+L L Sbjct: 242 HNGQNISSQHFQSALRDSYLLNN-KTKTVQDPLSFRNATLIHGAAYDSLSYVEEQLLLEL 300 Query: 328 NSSDDNPCVLSDEQRIVSCANYDPLSWTLGFEMLGSALHHVSKSSCHRIIKLGDPRFTGL 387 SSDDNP V D QRI+S +++ L+ +L EML AL HVS +S R+IKLG+P FTGL Sbjct: 301 ESSDDNPMVDIDNQRILSTPHFETLNVSLAIEMLNVALSHVSNASIQRMIKLGNPDFTGL 360 Query: 388 TRFLTADTSRSVGFCTVQKTATSLDAEIRHLSNPVSADYLSLAGEIEDHAANSPYVVSKT 447 +RFL AD + G +QKTA +LD EI H S +S LAGE+ED N P ++ + Sbjct: 361 SRFLIADEHQYFGLQALQKTAATLDVEIHHASQTLSNISYPLAGEMEDRQTNLPLILQEL 420 Query: 448 REILDRLTYILAIELLHAAQAVDLR 472 I+++L ILAIELL+A QA+ +R Sbjct: 421 DGIVNKLHEILAIELLYATQAIAIR 445 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,070,924,650 Number of extensions: 125186606 Number of successful extensions: 298969 Number of sequences better than 1.0e-05: 1275 Number of HSP's gapped: 295213 Number of HSP's successfully gapped: 1283 Length of query: 531 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 390 Effective length of database: 1,732,333,088 Effective search space: 675609904320 Effective search space used: 675609904320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)