BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0032.p (554 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5M3Q8.1 Q5M3Q8_STRT2 Urocanate hydratase 778 0.0 Q5LZ53.1 Q5LZ53_STRT1 Urocanate hydratase 778 0.0 Q63UC8.1 Q63UC8_BURPS Putative urocanate hydratase 730 0.0 Q3JS73.1 Q3JS73_BURP1 Urocanate hydratase EC=4.2.1.49 730 0.0 A3NVD7.1 A3NVD7_BURP0 Urocanate hydratase EC=4.2.1.49 730 0.0 >Q5M3Q8.1 Q5M3Q8_STRT2 Urocanate hydratase Length = 544 Score = 778 bits (2008), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/540 (67%), Positives = 450/540 (83%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVLENGENQRELIVYASLGKAARNWPSYLAIVDTLK 63 I A RG LR KGWRQEA+LR+LENVLEN E+ LIVY +LGKAAR+W SY AIV++LK Sbjct: 3 IKAQRGNQLRTKGWRQEAILRLLENVLENAEDPDNLIVYGALGKAARDWSSYEAIVESLK 62 Query: 64 RLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGGLT 123 +EEDE+L+IQSGKPIG+ KTH APLV+MAN NLVG+WATSENFY+L DK LI WGGLT Sbjct: 63 TIEEDESLIIQSGKPIGILKTHPDAPLVIMANSNLVGKWATSENFYDLYDKNLIAWGGLT 122 Query: 124 AAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGAAI 183 A WQYIGSQGVIQGTYEIF+SI+RL+F +L GR ILTAG+GGMGG+QPLA +AGA I Sbjct: 123 AGDWQYIGSQGVIQGTYEIFKSISRLYFSDDLSGRLILTAGMGGMGGSQPLAAKMAGANI 182 Query: 184 LCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPELLR 243 + V+V E+ IDKR++ GY+ +KTDSLD+AL+WI+ A+R GE+VS+ ++GNAA VY ++L Sbjct: 183 IVVDVNEDNIDKRLSIGYVDKKTDSLDQALSWIDDAKRKGENVSIAVIGNAATVYQDILD 242 Query: 244 RGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAMLQF 303 RG+ PD+V+DQT+AHDLLYGY+PEGY+ R+ +P +L++ AGASIA EV+A+L F Sbjct: 243 RGIVPDIVSDQTAAHDLLYGYVPEGYSLEEVIQVRQSNPKKLESDAGASIAKEVRAILSF 302 Query: 304 KERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQDI 363 + +GA VFDNGNNIR+QA + GV +AF+I IFTEAFLRPLF +AIGPFRWIALSG+P+DI Sbjct: 303 QNKGAKVFDNGNNIRTQAKRFGVENAFDIPIFTEAFLRPLFERAIGPFRWIALSGDPKDI 362 Query: 364 RIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVREGR 423 IID+ +L+ FSDN I+T+WIKLA ++PF+GLPARIGW GH++R++LA+AVN+LV EG Sbjct: 363 HIIDDYLLEHFSDNTIITDWIKLARKYVPFQGLPARIGWLGHEERTQLALAVNDLVAEGV 422 Query: 424 LAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSGGG 483 L+ PVAF+RDHLDA AMAHPNIMTE +KDGSDA++DWPLLNAML SS ADLVAIHSGGG Sbjct: 423 LSAPVAFTRDHLDAGAMAHPNIMTENLKDGSDAIADWPLLNAMLAASSKADLVAIHSGGG 482 Query: 484 GYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEIDRV 543 GY GYM SAG+T+VADGT ++ R++ ++ NDT LGVLRYADAGYE SL E K I RV Sbjct: 483 GYTGYMQSAGITVVADGTREASERVQLSITNDTSLGVLRYADAGYEASLDEIEQKRIRRV 542 >Q5LZ53.1 Q5LZ53_STRT1 Urocanate hydratase Length = 544 Score = 778 bits (2008), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/540 (67%), Positives = 450/540 (83%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVLENGENQRELIVYASLGKAARNWPSYLAIVDTLK 63 I A RG LR KGWRQEA+LR+LENVLEN E+ LIVY +LGKAAR+W SY AIV++LK Sbjct: 3 IKAQRGNQLRTKGWRQEAILRLLENVLENAEDPDNLIVYGALGKAARDWSSYEAIVESLK 62 Query: 64 RLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGGLT 123 +EEDE+L+IQSGKPIG+ KTH APLV+MAN NLVG+WATSENFY+L DK LI WGGLT Sbjct: 63 TIEEDESLIIQSGKPIGILKTHPDAPLVIMANSNLVGKWATSENFYDLYDKNLIAWGGLT 122 Query: 124 AAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGAAI 183 A WQYIGSQGVIQGTYEIF+SI+RL+F +L GR ILTAG+GGMGG+QPLA +AGA I Sbjct: 123 AGDWQYIGSQGVIQGTYEIFKSISRLYFSDDLSGRLILTAGMGGMGGSQPLAAKMAGANI 182 Query: 184 LCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPELLR 243 + V+V E+ IDKR++ GY+ +KTDSLD+AL+WI+ A+R GE+VS+ ++GNAA VY ++L Sbjct: 183 IVVDVNEDNIDKRLSIGYVDKKTDSLDQALSWIDDAKRKGENVSIAVIGNAATVYQDILD 242 Query: 244 RGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAMLQF 303 RG+ PD+V+DQT+AHDLLYGY+PEGY+ R+ +P +L++ AGASIA EV+A+L F Sbjct: 243 RGIVPDIVSDQTAAHDLLYGYVPEGYSLEEVIQVRQSNPKKLESDAGASIAKEVRAILSF 302 Query: 304 KERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQDI 363 + +GA VFDNGNNIR+QA + GV +AF+I IFTEAFLRPLF +AIGPFRWIALSG+P+DI Sbjct: 303 QNKGAKVFDNGNNIRTQAKRFGVENAFDIPIFTEAFLRPLFERAIGPFRWIALSGDPKDI 362 Query: 364 RIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVREGR 423 IID+ +L+ FSDN I+T+WIKLA ++PF+GLPARIGW GH++R++LA+AVN+LV EG Sbjct: 363 HIIDDYLLEHFSDNTIITDWIKLARKYVPFQGLPARIGWLGHEERTQLALAVNDLVAEGV 422 Query: 424 LAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSGGG 483 L+ PVAF+RDHLDA AMAHPNIMTE +KDGSDA++DWPLLNAML SS ADLVAIHSGGG Sbjct: 423 LSAPVAFTRDHLDAGAMAHPNIMTENLKDGSDAIADWPLLNAMLAASSKADLVAIHSGGG 482 Query: 484 GYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEIDRV 543 GY GYM SAG+T+VADGT ++ R++ ++ NDT LGVLRYADAGYE SL E K I RV Sbjct: 483 GYTGYMQSAGITVVADGTREASERVQLSITNDTSLGVLRYADAGYEASLDEIEQKRIRRV 542 >Q63UC8.1 Q63UC8_BURPS Putative urocanate hydratase Length = 546 Score = 730 bits (1885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/532 (67%), Positives = 434/532 (81%) Query: 9 GGALRCKGWRQEALLRMLENVLENGENQRELIVYASLGKAARNWPSYLAIVDTLKRLEED 68 G LRCKGWRQEALLR+LENVL GEN ELIVYA+LG+AAR+WPS+ AIV TL+ ++E+ Sbjct: 10 GDTLRCKGWRQEALLRLLENVLAVGENPDELIVYAALGRAARDWPSHDAIVRTLRTMDEN 69 Query: 69 ETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGGLTAAAWQ 128 +TLV+QSGKP+ + +TH APLVVMANCNLVG+WA +E+FYEL+ + LI WGGLTA WQ Sbjct: 70 QTLVVQSGKPVALLRTHASAPLVVMANCNLVGQWAKAEHFYELERRNLICWGGLTAGDWQ 129 Query: 129 YIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGAAILCVEV 188 YIGSQGVIQGTYEIF IA HFG +LRGRFILTAGLGGMGGAQPLAG +A AA L VE+ Sbjct: 130 YIGSQGVIQGTYEIFSRIAERHFGNDLRGRFILTAGLGGMGGAQPLAGRMANAATLVVEI 189 Query: 189 AEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPELLRRGVQP 248 + RI KR+ G+L+R+ LDEALA I A+ E +SVGLLGNAA+V+P +L RGV P Sbjct: 190 DQTRIHKRLQVGFLERQARDLDEALALIRDAQARREPISVGLLGNAADVFPAILARGVVP 249 Query: 249 DVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAMLQFKERGA 308 D+VTDQT+AHDL+YGY+P GYT A + R D A+L A+ ASI V+AML FK+RGA Sbjct: 250 DIVTDQTAAHDLVYGYVPSGYTLEEARSMRDTDRAKLMQASRASIVRHVEAMLGFKDRGA 309 Query: 309 IVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQDIRIIDE 368 IVFDNGN IR+ A GV AFEI +FTEAFLRPLF +AIGPFRWIALS +P DIR++D+ Sbjct: 310 IVFDNGNLIRTHAKDGGVARAFEIPVFTEAFLRPLFCRAIGPFRWIALSNDPNDIRVVDD 369 Query: 369 LILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVREGRLAGPV 428 +L+ F++N IVTNW++ A + +PFEGLPARI W GH +R+RLA+AVN LVRE LAGP+ Sbjct: 370 YLLEHFAENRIVTNWVRRARECVPFEGLPARIAWLGHGERTRLALAVNALVRERVLAGPI 429 Query: 429 AFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSGGGGYAGY 488 AF+RDHLDA AMAHPNIMTE ++DGSDAV+DWPLLNAM+NCSSMADLVAIHSGGGGY+GY Sbjct: 430 AFTRDHLDAGAMAHPNIMTENLRDGSDAVADWPLLNAMMNCSSMADLVAIHSGGGGYSGY 489 Query: 489 MTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEI 540 MTSAGVT+VADG+A + RL ++ NDT LGV+RYADAGYE +L EA ++I Sbjct: 490 MTSAGVTVVADGSAAADERLTLSMTNDTALGVIRYADAGYEEALDEAQRRQI 541 >Q3JS73.1 Q3JS73_BURP1 Urocanate hydratase EC=4.2.1.49 Length = 546 Score = 730 bits (1885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/532 (67%), Positives = 434/532 (81%) Query: 9 GGALRCKGWRQEALLRMLENVLENGENQRELIVYASLGKAARNWPSYLAIVDTLKRLEED 68 G LRCKGWRQEALLR+LENVL GEN ELIVYA+LG+AAR+WPS+ AIV TL+ ++E+ Sbjct: 10 GDTLRCKGWRQEALLRLLENVLAVGENPDELIVYAALGRAARDWPSHDAIVRTLRTMDEN 69 Query: 69 ETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGGLTAAAWQ 128 +TLV+QSGKP+ + +TH APLVVMANCNLVG+WA +E+FYEL+ + LI WGGLTA WQ Sbjct: 70 QTLVVQSGKPVALLRTHASAPLVVMANCNLVGQWAKAEHFYELERRNLICWGGLTAGDWQ 129 Query: 129 YIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGAAILCVEV 188 YIGSQGVIQGTYEIF IA HFG +LRGRFILTAGLGGMGGAQPLAG +A AA L VE+ Sbjct: 130 YIGSQGVIQGTYEIFSRIAERHFGNDLRGRFILTAGLGGMGGAQPLAGRMANAATLVVEI 189 Query: 189 AEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPELLRRGVQP 248 + RI KR+ G+L+R+ LDEALA I A+ E +SVGLLGNAA+V+P +L RGV P Sbjct: 190 DQTRIHKRLQVGFLERQARDLDEALALIRDAQARREPISVGLLGNAADVFPAILARGVVP 249 Query: 249 DVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAMLQFKERGA 308 D+VTDQT+AHDL+YGY+P GYT A + R D A+L A+ ASI V+AML FK+RGA Sbjct: 250 DIVTDQTAAHDLVYGYVPSGYTLEEARSMRDTDRAKLMQASRASIVRHVEAMLGFKDRGA 309 Query: 309 IVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQDIRIIDE 368 IVFDNGN IR+ A GV AFEI +FTEAFLRPLF +AIGPFRWIALS +P DIR++D+ Sbjct: 310 IVFDNGNLIRTHAKDGGVARAFEIPVFTEAFLRPLFCRAIGPFRWIALSNDPNDIRVVDD 369 Query: 369 LILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVREGRLAGPV 428 +L+ F++N IVTNW++ A + +PFEGLPARI W GH +R+RLA+AVN LVRE LAGP+ Sbjct: 370 YLLEHFAENRIVTNWVRRARECVPFEGLPARIAWLGHGERTRLALAVNALVRERVLAGPI 429 Query: 429 AFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSGGGGYAGY 488 AF+RDHLDA AMAHPNIMTE ++DGSDAV+DWPLLNAM+NCSSMADLVAIHSGGGGY+GY Sbjct: 430 AFTRDHLDAGAMAHPNIMTENLRDGSDAVADWPLLNAMMNCSSMADLVAIHSGGGGYSGY 489 Query: 489 MTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEI 540 MTSAGVT+VADG+A + RL ++ NDT LGV+RYADAGYE +L EA ++I Sbjct: 490 MTSAGVTVVADGSAAADERLTLSMTNDTALGVIRYADAGYEEALDEAQRRQI 541 >A3NVD7.1 A3NVD7_BURP0 Urocanate hydratase EC=4.2.1.49 Length = 546 Score = 730 bits (1885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/532 (67%), Positives = 434/532 (81%) Query: 9 GGALRCKGWRQEALLRMLENVLENGENQRELIVYASLGKAARNWPSYLAIVDTLKRLEED 68 G LRCKGWRQEALLR+LENVL GEN ELIVYA+LG+AAR+WPS+ AIV TL+ ++E+ Sbjct: 10 GDTLRCKGWRQEALLRLLENVLAVGENPDELIVYAALGRAARDWPSHDAIVRTLRTMDEN 69 Query: 69 ETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGGLTAAAWQ 128 +TLV+QSGKP+ + +TH APLVVMANCNLVG+WA +E+FYEL+ + LI WGGLTA WQ Sbjct: 70 QTLVVQSGKPVALLRTHASAPLVVMANCNLVGQWAKAEHFYELERRNLICWGGLTAGDWQ 129 Query: 129 YIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGAAILCVEV 188 YIGSQGVIQGTYEIF IA HFG +LRGRFILTAGLGGMGGAQPLAG +A AA L VE+ Sbjct: 130 YIGSQGVIQGTYEIFSRIAERHFGNDLRGRFILTAGLGGMGGAQPLAGRMANAATLVVEI 189 Query: 189 AEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPELLRRGVQP 248 + RI KR+ G+L+R+ LDEALA I A+ E +SVGLLGNAA+V+P +L RGV P Sbjct: 190 DQTRIHKRLQVGFLERQARDLDEALALIRDAQARREPISVGLLGNAADVFPAILARGVVP 249 Query: 249 DVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAMLQFKERGA 308 D+VTDQT+AHDL+YGY+P GYT A + R D A+L A+ ASI V+AML FK+RGA Sbjct: 250 DIVTDQTAAHDLVYGYVPSGYTLEEARSMRDTDRAKLMQASRASIVRHVEAMLGFKDRGA 309 Query: 309 IVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQDIRIIDE 368 IVFDNGN IR+ A GV AFEI +FTEAFLRPLF +AIGPFRWIALS +P DIR++D+ Sbjct: 310 IVFDNGNLIRTHAKDGGVARAFEIPVFTEAFLRPLFCRAIGPFRWIALSNDPNDIRVVDD 369 Query: 369 LILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVREGRLAGPV 428 +L+ F++N IVTNW++ A + +PFEGLPARI W GH +R+RLA+AVN LVRE LAGP+ Sbjct: 370 YLLEHFAENRIVTNWVRRARECVPFEGLPARIAWLGHGERTRLALAVNALVRERVLAGPI 429 Query: 429 AFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSGGGGYAGY 488 AF+RDHLDA AMAHPNIMTE ++DGSDAV+DWPLLNAM+NCSSMADLVAIHSGGGGY+GY Sbjct: 430 AFTRDHLDAGAMAHPNIMTENLRDGSDAVADWPLLNAMMNCSSMADLVAIHSGGGGYSGY 489 Query: 489 MTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEI 540 MTSAGVT+VADG+A + RL ++ NDT LGV+RYADAGYE +L EA ++I Sbjct: 490 MTSAGVTVVADGSAAADERLTLSMTNDTALGVIRYADAGYEEALDEAQRRQI 541 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,340,580,703 Number of extensions: 143999661 Number of successful extensions: 406396 Number of sequences better than 1.0e-05: 689 Number of HSP's gapped: 405134 Number of HSP's successfully gapped: 690 Length of query: 554 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 413 Effective length of database: 1,732,333,088 Effective search space: 715453565344 Effective search space used: 715453565344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)