BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0030.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0K6M1.1 B0K6M1_THEPX Substrate-binding region of ABC-type glyci... 225 5e-57 Q2RHV2.1 Q2RHV2_MOOTA Substrate-binding region of ABC-type glyci... 213 1e-53 A0Q392.1 A0Q392_CLONN Probable glycine betaine/carnitine/choline... 203 2e-50 C5D4L7.1 C5D4L7_9BACI Substrate-binding region of ABC-type glyci... 203 2e-50 B1YJ47.1 B1YJ47_EXIS2 Substrate-binding region of ABC-type glyci... 203 2e-50 >B0K6M1.1 B0K6M1_THEPX Substrate-binding region of ABC-type glycine betaine transport system Length = 304 Score = 225 bits (573), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 126/286 (44%), Positives = 182/286 (63%), Gaps = 10/286 (3%) Query: 34 CS-NSKASGSSDTIVLATKGFAESDILANAFKLLIEEDTDLKTEVKKLD---NTLLWNAI 89 C S ++DT+V+ +K F E I+AN +IE TDLK V+KL+ + + AI Sbjct: 21 CGIGSSQRNANDTVVVGSKNFTEQIIVANMVAEMIEAHTDLKV-VRKLNLGGTNVNFEAI 79 Query: 90 DTGD----VDAYVEYSGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNT 145 G +D YVEY+GT L++IL ++P DP+KA++TV + K+K N+ L+PLGFNNT Sbjct: 80 KRGGANNGIDIYVEYTGTGLVDILGMEPTTDPEKAYETVKKEYKDKWNIVWLKPLGFNNT 139 Query: 146 YVFAVRKETAEKYGLTTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSI 205 Y AV+ E A++Y L T S LA+ S +LI G++ EFL R D +P ++ +Y KFK KS+ Sbjct: 140 YTLAVKDELAKQYNLKTFSDLAKISDKLILGATMEFLERPDGYPGLQKVYNFKFKHTKSM 199 Query: 206 QNKSLQYQAIDQKLIDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHP 265 + ++Y AID + V+ ++TD L++ L LEDDKH F PY A PI+R L++HP Sbjct: 200 -DMGIRYTAIDNNEVQVIDAFATDGLLVSHKLKILEDDKHFFPPYYAAPIIRQDVLDKHP 258 Query: 266 ELKETLNKLAGKIDEATMQALNAEVEQEQKPAIDVAKEWLKSQALI 311 ELK+ LNKLA +I + MQ LN +V+ E + VAKE+LK + LI Sbjct: 259 ELKDVLNKLANQISDEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI 304 >Q2RHV2.1 Q2RHV2_MOOTA Substrate-binding region of ABC-type glycine betaine transport system Length = 304 Score = 213 bits (543), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 119/282 (42%), Positives = 174/282 (61%), Gaps = 6/282 (2%) Query: 33 GCSNSKASGSSDTIVLATKGFAESDILANAFKLLIEEDTDLKTEVK-KLDNTLL-WNAID 90 GCS ++A G T+V+ +K F E+ +L LIE TDLK E K L TL+ +NA+ Sbjct: 27 GCSGTRAKG---TVVVGSKDFTENILLGEIMAQLIEAHTDLKVERKLNLGGTLVNFNALK 83 Query: 91 TGDVDAYVEYSGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAV 150 GD+D Y +Y+GT L+ ILK D DP +A+ V E+ L L+P GFNNTY AV Sbjct: 84 KGDLDLYADYTGTGLVAILKRDVINDPQEAYDAVQKAYNEQFKLKWLKPFGFNNTYALAV 143 Query: 151 RKETAEKYGLTTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSL 210 +E A + L S L +GE++ G+ +EF NR D + + Y FK K ++ L Sbjct: 144 PEEVARQRNLQKISDLKSVAGEMVLGAEQEFFNRPDGYDGLIVTYGLNFKSTKQMET-GL 202 Query: 211 QYQAIDQKLIDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKET 270 +Y+AI K++DV+ ++TD QL+T L LEDDK F PY A P+VR TLE++P+L+E Sbjct: 203 KYEAIHNKMVDVIDAFATDGQLITYKLKILEDDKQFFPPYFAAPLVRMDTLEKYPQLEEV 262 Query: 271 LNKLAGKIDEATMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 LNKLAG++++ M+ LN +V++E+K VA+++L + LIK Sbjct: 263 LNKLAGQLNDDEMRQLNYQVDEEKKEVAQVARDFLLKKGLIK 304 >A0Q392.1 A0Q392_CLONN Probable glycine betaine/carnitine/choline ABC transporter Length = 528 Score = 203 bits (516), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 111/271 (40%), Positives = 170/271 (62%), Gaps = 4/271 (1%) Query: 45 TIVLATKGFAESDILANAFKLLIEEDTDLKTEVKKLD---NTLLWNAIDTGDVDAYVEYS 101 +IV+ +K + E IL N + L+EE+TDLK + ++ + + A+ +GD+D YVEY+ Sbjct: 259 SIVVGSKNYTEELILGNMYATLLEENTDLKVKRTGINLGGAGVTFQALRSGDIDLYVEYT 318 Query: 102 GTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGLT 161 G AL+NI+ DP+K F+ V ++ ++T ++ +G+NNTYV AV KETA+KYGL Sbjct: 319 GVALVNIMGRKAINDPEKTFKIVKDYFNKEYDITWMDQMGYNNTYVLAVAKETAKKYGLK 378 Query: 162 TASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSLQYQAIDQKLID 221 T S LA+ S L+ G + EF +R D + +++ Y K++KS+ + SL+Y A+ K D Sbjct: 379 TLSDLAKVSNNLVLGCTMEFSDRQDGYLGMKDFYNMNVKDVKSL-DGSLRYSALQSKEAD 437 Query: 222 VMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKIDEA 281 V+ ++TD L L LEDDKH F PY A PI+R L++HPELK+ LN LAGK+ E Sbjct: 438 VIDAFATDGLLKVYNLTRLEDDKHFFPPYYAAPIIRNDVLKKHPELKKVLNMLAGKVSED 497 Query: 282 TMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 M LN +V++ + VA ++L+ + LIK Sbjct: 498 DMTELNYKVDKLGEDPRKVADDFLRKRKLIK 528 >C5D4L7.1 C5D4L7_9BACI Substrate-binding region of ABC-type glycine betaine transport system Length = 300 Score = 203 bits (516), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 118/270 (43%), Positives = 169/270 (62%), Gaps = 5/270 (1%) Query: 46 IVLATKGFAESDILANAFKLLIEEDTDLKTEVK-KLDNT-LLWNAIDTGDVDAYVEYSGT 103 IV+A+K F E ILA+ ++ +TDL E+K L +L A++ GDVD YVEY+GT Sbjct: 31 IVVASKKFTEQVILAHLLAEYLKANTDLDVEMKDSLGGAFVLQQAMEKGDVDLYVEYTGT 90 Query: 104 ALINILK--LDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGLT 161 +NILK DP P++ ++ Q KEK N+ L+PLGFNNTY A+RKE A+K G+ Sbjct: 91 GHLNILKNKYDPSKTPEEIYEETKKQYKEKFNIAWLKPLGFNNTYALAMRKELADKLGVK 150 Query: 162 TASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSLQYQAIDQKLID 221 T S LA+KS +L+FG+ EF R+D + + + Y KFK+ +I + LQY+A ID Sbjct: 151 TYSDLAKKSDQLVFGTDAEFFERSDGYDGLVDAYGFKFKKTANI-DPDLQYEAAKNGDID 209 Query: 222 VMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKIDEA 281 V+ Y+TD+++ LV L+DDKH F PY A PI+R + L+ +P L + LNKL G + + Sbjct: 210 VITAYTTDARIKKYNLVVLKDDKHYFPPYHAVPIIRQEVLDANPGLGKVLNKLEGILTDE 269 Query: 282 TMQALNAEVEQEQKPAIDVAKEWLKSQALI 311 M LNA+V E K VA ++LK++ LI Sbjct: 270 KMMELNAQVNMEGKRPKQVAIDFLKAEGLI 299 >B1YJ47.1 B1YJ47_EXIS2 Substrate-binding region of ABC-type glycine betaine transport system Length = 527 Score = 203 bits (516), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 121/273 (44%), Positives = 169/273 (61%), Gaps = 4/273 (1%) Query: 43 SDTIVLATKGFAESDILANAFKLLIEEDTDLKTEVK-KLDNT-LLWNAIDTGDVDAYVEY 100 +D IV+ +K F E IL N LIE+ TDL+ E + L T + + A++TG++DAYVEY Sbjct: 255 TDKIVIGSKNFTEQLILGNMLADLIEDKTDLEVERQLNLGGTKVAYGALETGEIDAYVEY 314 Query: 101 SGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGL 160 +GT LI++LK E DP+ ++ V + EK+ + LEP+GFNNTY A+ KE +KY L Sbjct: 315 TGTLLIDVLKQKLETDPEAVYKKVGKMIPEKSQVDVLEPIGFNNTYALAMTKEDIKKYDL 374 Query: 161 TTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYK-PKFKEIKSIQNKSLQYQAIDQKL 219 T S +A S LI GS+ EF NR D + ++ Y KFK+++ + + L+Y A+ Sbjct: 375 KTVSDMAGVSNRLILGSTIEFANREDGYLGLKEKYPDMKFKDVQPV-DGGLRYTALTNGK 433 Query: 220 IDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKID 279 +V+ +STD L L LEDDK F PY A PIVR +TLEEHPELKE LN L KI Sbjct: 434 TNVIDSFSTDGLLKKFDLTVLEDDKEFFPPYYAVPIVRQETLEEHPELKEVLNLLKDKIT 493 Query: 280 EATMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 + MQ LN + + E++ DVA+E+L + LIK Sbjct: 494 DKKMQELNFKADVEKERPEDVAREFLIEEELIK 526 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,630,741,181 Number of extensions: 64279369 Number of successful extensions: 169517 Number of sequences better than 1.0e-05: 917 Number of HSP's gapped: 167426 Number of HSP's successfully gapped: 925 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 131 (55.1 bits)