BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0011.p (486 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIS8.1 B3KIS8_9BACI PTS system, N-acetylglucosamine-specific I... 730 0.0 C0ZH74.1 C0ZH74_BREBN PTS system IIBC component EC=2.7.1.69 626 e-177 B1D7P9.1 B1D7P9_9BACL PTS system, N-acetylglucosamine-specific I... 596 e-168 Q7P0K8.1 Q7P0K8_CHRVO Protein-N p-phosphohistidine-sugar phospho... 443 e-122 B0KCA5.1 B0KCA5_THEP3 PTS system, N-acetylglucosamine-specific I... 409 e-112 >B3KIS8.1 B3KIS8_9BACI PTS system, N-acetylglucosamine-specific IIBC subunit Length = 489 Score = 730 bits (1885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/490 (73%), Positives = 414/490 (84%), Gaps = 8/490 (1%) Query: 4 MLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGA 63 MLA LQKLGKSLMLPVATLPAA ILQG LI+Y+KD LGA+G F+N Y+ PF+ +GA A Sbjct: 1 MLAKLQKLGKSLMLPVATLPAAGILQGLGLIDYQKDIPLGALGAFLNQYVTPFMTSGALA 60 Query: 64 IFGNLALIFAIGVAIGFAGDAVAALSALIAYQILDSILKVIPAQMPFIGDDVKLNMGVLG 123 I NL +IFAIGVAIGFAGDAVA LSALI Y +L +L +P QMPFI DDVKLNMGVLG Sbjct: 61 ILDNLPIIFAIGVAIGFAGDAVAGLSALIGYMVLTRVLAQVPLQMPFIPDDVKLNMGVLG 120 Query: 124 GIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDVIS 183 G FVG W+A+LY K+HNIKMPDWLGFFAGKRFVPIITA +T+VL+VL+GMIWSPIQD IS Sbjct: 121 GFFVGLWSAYLYNKFHNIKMPDWLGFFAGKRFVPIITAASTMVLAVLIGMIWSPIQDGIS 180 Query: 184 DFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWRFF 243 FG WVVGLGG+G+FIFGTANRLLIP GLHHV+N+IAWFQIGD+TNAAGEVVHGDLWRFF Sbjct: 181 AFGNWVVGLGGIGSFIFGTANRLLIPLGLHHVLNSIAWFQIGDYTNAAGEVVHGDLWRFF 240 Query: 244 AGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITEPL 303 AGD +AGMFM+GFFPIMMFA+P AALAF+HTAKP KRK+V SIFIGSA+ASFLTGITEPL Sbjct: 241 AGDPTAGMFMSGFFPIMMFAMPAAALAFMHTAKPSKRKLVGSIFIGSAVASFLTGITEPL 300 Query: 304 EFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWVLIPV 363 EF+FMFIAPVLY+VHA+LTG +G +MYVLDV LGF FSAGLIDYL+N+KLSTNAW+L+PV Sbjct: 301 EFAFMFIAPVLYVVHAILTGLSGLIMYVLDVHLGFSFSAGLIDYLVNLKLSTNAWLLLPV 360 Query: 364 GLGFFVLYYVLFRFIITKFNLKTPGRXXXXXXX-------XXXXXVSGAKISASSRAVKI 416 GL F V+YY+LFR +I K NLKTPGR SGA S S+A K+ Sbjct: 361 GLAFGVVYYLLFRILIVKLNLKTPGREDDDDMDPISDDGDTVAPAPSGAA-SNESKAAKV 419 Query: 417 LENVGGPSNIRNIDACITRLRLIVNDEKAVKDAALKQLGASGVMRLGQGAVQIVFGPQSE 476 L+N+GG NI +IDACITRLRL+V DEKAVKD+ LK+LGASGVMRLGQGAVQ+VFGPQ+E Sbjct: 420 LQNIGGADNITSIDACITRLRLVVKDEKAVKDSELKKLGASGVMRLGQGAVQVVFGPQAE 479 Query: 477 QIKEDINKLL 486 IK++I K++ Sbjct: 480 SIKDEIKKMM 489 >C0ZH74.1 C0ZH74_BREBN PTS system IIBC component EC=2.7.1.69 Length = 479 Score = 626 bits (1614), Expect = e-177, Method: Compositional matrix adjust. Identities = 316/482 (65%), Positives = 391/482 (81%), Gaps = 3/482 (0%) Query: 4 MLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGA 63 MLAFLQK+G++LMLPVATLPAAAILQ F L+ +EKD +LGA G F++TYIAP L AG A Sbjct: 1 MLAFLQKIGRALMLPVATLPAAAILQSFGLLKFEKDLNLGAFGAFMDTYIAPLLAAGGSA 60 Query: 64 IFGNLALIFAIGVAIGFAGDAVAALSALIAYQILDSILKVIPAQMPFIGDDVKLNMGVLG 123 IF NLALIFAIGVAIG AGDAVAAL+A+IAY +L +L +PA MPFI DDVKLNMGV+G Sbjct: 61 IFDNLALIFAIGVAIGLAGDAVAALAAVIAYVVLTKVLAAVPAAMPFIADDVKLNMGVIG 120 Query: 124 GIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDVIS 183 GI GA AAF Y +YH+IK+P+WLGFF GKRFVPI+T+++ +V+ ++ G+IW PIQ + Sbjct: 121 GILAGAIAAFFYNRYHSIKLPEWLGFFGGKRFVPIVTSLSMVVVGLIFGIIWGPIQIALD 180 Query: 184 DFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWRFF 243 G+W+VGLG G+F FG NRLL+PFGLHHV+NAIAWFQIGDFT+A G+VVHGDL RFF Sbjct: 181 SLGSWIVGLGATGSFFFGFFNRLLVPFGLHHVLNAIAWFQIGDFTDATGKVVHGDLHRFF 240 Query: 244 AGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITEPL 303 AGD SAGMFMTGF+PIMMFALPGAA A IH+A+PEKRK+VAS+FIG+A+ASFLTGITE + Sbjct: 241 AGDPSAGMFMTGFYPIMMFALPGAAFAIIHSARPEKRKVVASMFIGAALASFLTGITEVV 300 Query: 304 EFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWVLIPV 363 EF+FMF AP+LY VHA+LTG +G+++ L +K GFGFSAGLIDY +N LSTNAW++IP+ Sbjct: 301 EFAFMFAAPLLYFVHAILTGVSGYIVTSLGIKHGFGFSAGLIDYGINYHLSTNAWMIIPI 360 Query: 364 GLGFFVLYYVLFRFIITKFNLKTPGRXXXXXXXXXXXXVSGAKISASSRAVKILENVGGP 423 GL F V+YY LFRF+I K NLKTPGR +G S +A+++L ++GG Sbjct: 361 GLAFAVIYYALFRFLIVKLNLKTPGR---EDDEEMTTTATGRNNSMQDKAIQVLTHIGGK 417 Query: 424 SNIRNIDACITRLRLIVNDEKAVKDAALKQLGASGVMRLGQGAVQIVFGPQSEQIKEDIN 483 N+ ++DACITRLRLI+ D+K V D ALK LGA+GVMRLGQG+VQ+VFG SE +KE+I+ Sbjct: 418 ENVVSVDACITRLRLILKDDKKVNDKALKDLGAAGVMRLGQGSVQVVFGTHSELLKEEIS 477 Query: 484 KL 485 KL Sbjct: 478 KL 479 >B1D7P9.1 B1D7P9_9BACL PTS system, N-acetylglucosamine-specific IIBC subunit Length = 483 Score = 596 bits (1536), Expect = e-168, Method: Compositional matrix adjust. Identities = 316/489 (64%), Positives = 376/489 (76%), Gaps = 12/489 (2%) Query: 4 MLAFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGA 63 MLAFLQK+GKSLMLPVA LPAAA+L F I+Y KDF G G F+N YIAPFL AG A Sbjct: 1 MLAFLQKIGKSLMLPVAALPAAALLLRFGNIDYVKDFGFGQFGDFLNHYIAPFLAAGGSA 60 Query: 64 IFGNLALIFAIGVAIGFAGDAVAALSALIAYQILDSILKVIPAQMP-FIGDDVKLNMGVL 122 IF NL LIFAIGVAIG AGDAVA L+A+IAY +L ++L IP + D LNMGVL Sbjct: 61 IFDNLPLIFAIGVAIGMAGDAVATLAAVIAYLVLTNVLGKIPTVFTGLVDKDTTLNMGVL 120 Query: 123 GGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDVI 182 GGI G A++ Y KYH+IK+PDWLGFF GKRFVPIIT+ +++ ++ G+IW P+Q + Sbjct: 121 GGIIAGLVASYFYNKYHDIKLPDWLGFFGGKRFVPIITSFAMVIVGLIFGLIWGPVQSWL 180 Query: 183 SDFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWRF 242 FG W+V LGGVGAFIF TANRLLIPFGLHHVINAIAWFQIGD+T+AAG VVHGDL RF Sbjct: 181 DTFGRWIVDLGGVGAFIFMTANRLLIPFGLHHVINAIAWFQIGDYTDAAGNVVHGDLHRF 240 Query: 243 FAGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITEP 302 FAGD +AGMFMTGFFPIMMFALP AA A IHTAKPEKRK+V+SIFIGSA+ASFLTGITEP Sbjct: 241 FAGDPTAGMFMTGFFPIMMFALPAAAFAIIHTAKPEKRKLVSSIFIGSALASFLTGITEP 300 Query: 303 LEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWVLIP 362 LEF+FMF+APVLY++HA+LTG + +L+ L + GFGFSAG +DY +N LST W++IP Sbjct: 301 LEFAFMFVAPVLYVIHAVLTGISAWLVVELGIHHGFGFSAGFLDYAINYPLSTKGWMIIP 360 Query: 363 VGLGFFVLYYVLFRFIITKFNLKTPGRXXXXXXXXXXXXVSGAKISA-----SSRAVKIL 417 +GL + V+YYVLFR +I K NLKTPGR GAK +A +A ++L Sbjct: 361 IGLVYAVVYYVLFRVVIVKLNLKTPGREDDDLVE------EGAKDAAPAGAGDDKASQVL 414 Query: 418 ENVGGPSNIRNIDACITRLRLIVNDEKAVKDAALKQLGASGVMRLGQGAVQIVFGPQSEQ 477 +GG NI +IDACITRLRLIV DEKAV DA LK+LGA+GVMRLG+GAVQ +FG QSE+ Sbjct: 415 AALGGSGNIESIDACITRLRLIVKDEKAVNDAELKRLGAAGVMRLGKGAVQAIFGTQSER 474 Query: 478 IKEDINKLL 486 +KE I K + Sbjct: 475 LKEQIKKRM 483 >Q7P0K8.1 Q7P0K8_CHRVO Protein-N p-phosphohistidine-sugar phosphotransferase EC=2.7.1.69 Length = 562 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 235/484 (48%), Positives = 317/484 (65%), Gaps = 35/484 (7%) Query: 6 AFLQKLGKSLMLPVATLPAAAILQGFALINYEKDFHLGAIGRFINTYIAPFLNAGAGAIF 65 A +Q+LG++LMLP+A LP A +L LG + F+ AIF Sbjct: 8 AGIQQLGRALMLPIAVLPVAGLL-----------LRLGQP----DLLDIKFMAQAGDAIF 52 Query: 66 GNLALIFAIGVAIGFA--GDAVAALSALIAYQILDSILKVIPAQMPFIGDDVKLNMGVLG 123 GNLALIFAIGVA+GFA G+ + L+ I Y +L ++LKVI D K+NMGVL Sbjct: 53 GNLALIFAIGVAVGFAKDGNGTSGLAGAIGYLVLTAVLKVI---------DDKINMGVLS 103 Query: 124 GIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGMIWSPIQDVIS 183 GI G A +LY +Y +IK+P +L FF GKRFVPI+T +VL ++G W P+Q I Sbjct: 104 GILSGLIAGYLYNRYKDIKLPSYLAFFGGKRFVPIVTGFGMLVLGAVLGYAWPPVQHGID 163 Query: 184 DFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGEVVHGDLWRFF 243 G W++ G VG F +G NR+LI GLHH++N I WFQ+GDFTNAAG+VVHGDL RFF Sbjct: 164 AVGKWLIATGEVGVFFYGVLNRILIVTGLHHILNNIVWFQVGDFTNAAGKVVHGDLTRFF 223 Query: 244 AGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIASFLTGITEPL 303 AGDK+AGMFM G FP+MMF LP A LA TAKPE R V + A+ + LTG+TEP+ Sbjct: 224 AGDKTAGMFMAGMFPVMMFGLPAACLAMYRTAKPENRAAVGGLLFSMALTAMLTGVTEPV 283 Query: 304 EFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKLSTNAWVLIPV 363 EF+FMF+APVLY +HA+LTG + +M +L VKLGFGFSAGL DY++N ++T +L+PV Sbjct: 284 EFAFMFLAPVLYGIHAVLTGVSMAVMQMLGVKLGFGFSAGLFDYVINYGIATKPLLLLPV 343 Query: 364 GLGFFVLYYVLFRFIITKFNLKTPGRXXXXXXXXXXXXVSGAKISA-SSRAVKILENVGG 422 G +F +YY LF F I KF+LKTPGR V+ A++ A +SRA ++ +GG Sbjct: 344 GAVYFAVYYALFVFFIRKFDLKTPGR--------EDAAVTVAEVKAGTSRARAFIDALGG 395 Query: 423 PSNIRNIDACITRLRLIVNDEKAVKDAALKQLGASGVMRLGQGAVQIVFGPQSEQIKEDI 482 +N++++DAC TRLRL V + V +AALK LG+ G+++ G+VQ+V GP+++ I ++I Sbjct: 396 AANLKSVDACTTRLRLQVASNEKVNEAALKALGSRGLIKPAAGSVQVVLGPEADLIADEI 455 Query: 483 NKLL 486 L Sbjct: 456 RGAL 459 >B0KCA5.1 B0KCA5_THEP3 PTS system, N-acetylglucosamine-specific IIBC subunit Length = 466 Score = 409 bits (1052), Expect = e-112, Method: Compositional matrix adjust. Identities = 220/431 (51%), Positives = 302/431 (70%), Gaps = 13/431 (3%) Query: 55 PFLNAGAGAIFGNLALIFAIGVAIGFA-GDAVAALSALIAYQILDSILKVIPAQMPFIGD 113 PF+ A+F NL LIFAIG+AIGFA GD VAAL+A + Y +L I Sbjct: 39 PFIMQAGAAVFDNLPLIFAIGIAIGFAEGDGVAALAAAVGYFVLTKGATTI--------- 89 Query: 114 DVKLNMGVLGGIFVGAWAAFLYKKYHNIKMPDWLGFFAGKRFVPIITAVTTIVLSVLVGM 173 + +NMGVLGGI +G A +LY KYH+IK+PD+LGFF GKRFVPI+TA IVL++++G Sbjct: 90 NKDINMGVLGGILMGIIAGYLYNKYHDIKLPDFLGFFGGKRFVPIVTAFAAIVLALVMGY 149 Query: 174 IWSPIQDVISDFGTWVVGLGGVGAFIFGTANRLLIPFGLHHVINAIAWFQIGDFTNAAGE 233 +W PIQ+ I G W++G G +G F++G NRLLIPFGLHHVIN++ WF G F AAGE Sbjct: 150 VWPPIQNGIYALGEWIIGAGALGVFVYGVLNRLLIPFGLHHVINSLVWFVFGTFKTAAGE 209 Query: 234 VVHGDLWRFFAGDKSAGMFMTGFFPIMMFALPGAALAFIHTAKPEKRKMVASIFIGSAIA 293 VV GDL RFFAGD +AG+FM GF+PIMMF LP AALA A+P +RK+V+ + I +A+ Sbjct: 210 VVTGDLNRFFAGDPTAGIFMAGFYPIMMFGLPAAALAMWAAARPNQRKVVSGVLISAALT 269 Query: 294 SFLTGITEPLEFSFMFIAPVLYLVHALLTGFAGFLMYVLDVKLGFGFSAGLIDYLLNMKL 353 + LTGITEP+EF+FMF+APVLY++HALLTG + + Y+L +K GFGFSAGLIDY+L+ + Sbjct: 270 ALLTGITEPIEFAFMFLAPVLYVIHALLTGLSLAITYILGIKAGFGFSAGLIDYVLSYGI 329 Query: 354 STNAWVLIPVGLGFFVLYYVLFRFIITKFNLKTPGRXXXXXXXXXXXXVSGAKISASSRA 413 ST +L+ +G+ + +YYV+F ++I KFNL TPGR +K A Sbjct: 330 STKPLLLLLIGIIYGAIYYVIFYYVIVKFNLPTPGRLEEEATDQYKEL---SKSEIGGIA 386 Query: 414 VKILENVGGPSNIRNIDACITRLRLIVNDEKAVKDAALKQLGASGVMRLGQGAVQIVFGP 473 + +E +GG NI++++ACITRLRL V D+ + D LK+LGA+GVMR+G+ A+Q++ G Sbjct: 387 AQYVEVLGGVENIQSLEACITRLRLTVKDDTIIDDDKLKKLGATGVMRMGKNALQVIVGT 446 Query: 474 QSEQIKEDINK 484 +++ I +++ K Sbjct: 447 KADLIAQEMKK 457 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.145 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,781,531,155 Number of extensions: 118861592 Number of successful extensions: 425048 Number of sequences better than 1.0e-05: 1772 Number of HSP's gapped: 416999 Number of HSP's successfully gapped: 1903 Length of query: 486 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 346 Effective length of database: 1,741,754,103 Effective search space: 602646919638 Effective search space used: 602646919638 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)