BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0010.p (171 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIS7.1 B3KIS7_9BACI PTS system, glucose subfamily, IIA subunit 203 6e-51 B1D7P8.1 B1D7P8_9BACL PTS system, glucose subfamily, IIA subunit 163 7e-39 B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit 145 2e-33 C1QEC9.1 C1QEC9_9SPIR PTS system IIA component; PTS system IIB c... 139 8e-32 B0A7C2.1 B0A7C2_9CLOT Putative uncharacterized protein 138 1e-31 >B3KIS7.1 B3KIS7_9BACI PTS system, glucose subfamily, IIA subunit Length = 165 Score = 203 bits (516), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 99/167 (59%), Positives = 130/167 (77%), Gaps = 6/167 (3%) Query: 2 MFSKWKSKKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIA 61 MF +WK KK A T +++ AP+SG+AV L+ VPDE F+ GHMGQGIAIEPSEGKLIA Sbjct: 1 MFDRWKKKKTA-----TVIQLTAPLSGKAVPLSQVPDEAFAAGHMGQGIAIEPSEGKLIA 55 Query: 62 PFDGKIAHVVKSSHAVIIEH-SSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLI 120 PFDG +AHV+KS HAV++E ++GLQ L HIG++TV LKG GF SH+ +GD+VK+GQTLI Sbjct: 56 PFDGTVAHVIKSKHAVMLEDPATGLQYLFHIGINTVGLKGEGFTSHVQAGDQVKSGQTLI 115 Query: 121 EFDIASIQAAGYQTITPVIVTRNGEADPEVVCRYGSVTAGKDIILTV 167 EFD+A+I+AAGY TITP+IVT E +V + G V +G++ +LT+ Sbjct: 116 EFDMAAIRAAGYPTITPIIVTNADEVASKVDGQTGDVVSGQNPVLTI 162 >B1D7P8.1 B1D7P8_9BACL PTS system, glucose subfamily, IIA subunit Length = 161 Score = 163 bits (412), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 80/156 (51%), Positives = 112/156 (71%), Gaps = 7/156 (4%) Query: 5 KWKSKKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFD 64 KW KK ++ + AP+SG+ V L+ VPDE F+ G MG+G+AIEP+EG++IAPFD Sbjct: 2 KWFGKKS------NSVSVAAPLSGKLVPLSEVPDEAFAQGLMGEGVAIEPTEGRVIAPFD 55 Query: 65 GKIAHVVKSSHAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDI 124 G ++H++ + HA+IIEHSSGLQLL+H+G++TV+L G GF + + +GD KAGQTL+E D+ Sbjct: 56 GVVSHLIDTHHALIIEHSSGLQLLIHVGINTVALNGDGFKALVKTGDSFKAGQTLLECDL 115 Query: 125 ASIQAAGYQTITPVIVTRNGEADPEVVCRYGSVTAG 160 I+ AGY ITPVIV N E +V C + +V AG Sbjct: 116 DKIRDAGYPVITPVIVA-NEEGAEKVECTFKTVNAG 150 >B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit Length = 678 Score = 145 bits (365), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 70/141 (49%), Positives = 101/141 (71%), Gaps = 6/141 (4%) Query: 6 WKSKKEAQPSKITT-----LEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLI 60 + S + A PS + +E+ AP+ G+ V L+ VPD F+ MGQGIAI PSEGK+ Sbjct: 505 FASTETANPSTVEAEQQGAVEVKAPIKGKVVPLSEVPDPAFADKQMGQGIAIIPSEGKVY 564 Query: 61 APFDGKIAHVVKSS-HAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTL 119 APFDG++AH++K S HA+I+ ++G+Q+L+H+G++TVSLKG GF +H+ +G E++ GQ L Sbjct: 565 APFDGQVAHLIKKSKHAIILTDANGIQILIHVGINTVSLKGEGFTAHVETGAEIRKGQLL 624 Query: 120 IEFDIASIQAAGYQTITPVIV 140 +EFDI IQ AG ITPVI+ Sbjct: 625 LEFDIDRIQQAGLPVITPVII 645 >C1QEC9.1 C1QEC9_9SPIR PTS system IIA component; PTS system IIB component; PTS system IIC component Length = 724 Score = 139 bits (351), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 71/156 (45%), Positives = 96/156 (61%) Query: 9 KKEAQPSKITTLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFDGKIA 68 KK + ++ AP+ G V L V DE FS G MG+GIAIEP EGK+ APFDG I Sbjct: 565 KKSENKAAALVDKVYAPIQGNIVKLESVKDEAFSSGAMGKGIAIEPKEGKVYAPFDGIIE 624 Query: 69 HVVKSSHAVIIEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDIASIQ 128 + HA+ + G++LL+H+GMDTV L G+ FISHI G ++K G L+EF+I I+ Sbjct: 625 TAFPTKHAIGLTSDKGVELLIHVGMDTVKLGGAHFISHIEEGQKIKKGDLLLEFNIEKIR 684 Query: 129 AAGYQTITPVIVTRNGEADPEVVCRYGSVTAGKDII 164 AGY T+TPVIVT + + + SV AG D++ Sbjct: 685 GAGYPTLTPVIVTNSDDYSEVGITSASSVNAGDDLL 720 >B0A7C2.1 B0A7C2_9CLOT Putative uncharacterized protein Length = 650 Score = 138 bits (348), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 66/146 (45%), Positives = 98/146 (67%) Query: 19 TLEILAPVSGQAVSLTGVPDETFSGGHMGQGIAIEPSEGKLIAPFDGKIAHVVKSSHAVI 78 T EI +P+SG+ ++L+ + D F+GG MG GIAI+PS+ K+ AP DG + V S HAV Sbjct: 498 TKEIKSPLSGKVIALSKIDDPVFAGGAMGNGIAIQPSDNKVYAPLDGTVEFVADSKHAVG 557 Query: 79 IEHSSGLQLLLHIGMDTVSLKGSGFISHIASGDEVKAGQTLIEFDIASIQAAGYQTITPV 138 I+ G++LL+H+GMDTV + G GF + + D+VKAG L+EFD +I+ AGY ITPV Sbjct: 558 IKSDDGVELLIHVGMDTVQMNGKGFDVKVNANDKVKAGDLLLEFDREAIEKAGYSLITPV 617 Query: 139 IVTRNGEADPEVVCRYGSVTAGKDII 164 ++T + E + + +C ++ GK II Sbjct: 618 VITNSFEFEQKELCLDEDISYGKSII 643 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,006,632,918 Number of extensions: 39751314 Number of successful extensions: 106779 Number of sequences better than 1.0e-05: 1949 Number of HSP's gapped: 106401 Number of HSP's successfully gapped: 1949 Length of query: 171 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 45 Effective length of database: 1,873,648,313 Effective search space: 84314174085 Effective search space used: 84314174085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)