BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0004.p (50 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIS2.1 B3KIS2_9BACI Sporulation protein, yteA family 65 1e-09 >B3KIS2.1 B3KIS2_9BACI Sporulation protein, yteA family Length = 187 Score = 65.5 bits (158), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 32/46 (69%), Positives = 37/46 (80%), Gaps = 2/46 (4%) Query: 1 MTAPPAGAGRNRQQHSGRFDDAGAWDAVEDYGSASSPVTS--PGQD 44 MT PP+GAG NRQ G+FDDAGAWDAVEDYG+++SP S PGQD Sbjct: 136 MTPPPSGAGVNRQAADGKFDDAGAWDAVEDYGTSTSPGMSIKPGQD 181 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.306 0.125 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 372,743,584 Number of extensions: 11862733 Number of successful extensions: 13780 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 13780 Number of HSP's successfully gapped: 1 Length of query: 50 Length of database: 3,060,696,203 Length adjustment: 23 Effective length of query: 27 Effective length of database: 2,844,012,858 Effective search space: 76788347166 Effective search space used: 76788347166 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 126 (53.1 bits)