BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0007.p (318 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAX0.1 B3KAX0_9BACI Cobalamin biosynthesis protein CobD 286 2e-75 C0Z7H8.1 C0Z7H8_BREBN Cobalamin biosynthesis protein 269 3e-70 A5D2P5.1 A5D2P5_PELTS Cobalamin biosynthesis protein CobD/CbiB 238 4e-61 C1TIH3.1 C1TIH3_9FIRM Adenosylcobinamide-phosphate synthase EC=6... 217 1e-54 B7B1T7.1 B7B1T7_9FIRM Putative uncharacterized protein 213 2e-53 >B3KAX0.1 B3KAX0_9BACI Cobalamin biosynthesis protein CobD Length = 325 Score = 286 bits (731), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 151/303 (49%), Positives = 195/303 (64%), Gaps = 3/303 (0%) Query: 6 ILLAAYVLDRIIGDPRSIPHPVVLMGKAVTAIERLIRRFCSQPRXXXXXXXXXXXXXXXX 65 ++ AA LD +IGDPR +PHPV+ MGKA+ +E IR+ + + Sbjct: 6 VIWAAVALDLLIGDPRWLPHPVIGMGKAIRRVEGWIRQRTTSAQGLKRAGILLPVIVAGG 65 Query: 66 XXXXTAVILLLLSRIAPWLAWAAEVWLISTTIASKGLKDAGMAVYAELRKGDIPAARTAL 125 ++L L + I PWLAW AE+ LI+TTIA KGL++AG+ VY LR G+I AR AL Sbjct: 66 SFVLAWLLLELCAAIHPWLAWGAEIVLIATTIAIKGLREAGLEVYGHLRSGNIAEARQAL 125 Query: 126 GMIVGRDTTKLESPDIVRGTVETVAENIVDAIISXXXXXXXXXXXXXXXYRAVNTLDSMV 185 M+VGRDT L+ P+IVRGTVETVAENIVDA+I+ YRAVNTLDSMV Sbjct: 126 SMVVGRDTAHLDEPEIVRGTVETVAENIVDAVIAPLFFALIGGAPLAMAYRAVNTLDSMV 185 Query: 186 GYKNDKYRNLGWASARLDDVANYIPARMTAPLLVLCAWLLRLDWRSSWRIVRRDARLHPS 245 GYKN+KY +LGWASARLDD+AN+IPAR+TA LL + A + L W+ + ++V+RDAR HPS Sbjct: 186 GYKNEKYLHLGWASARLDDLANWIPARLTAGLLTVAAAMFSLQWKQAIKMVKRDARKHPS 245 Query: 246 PNS---XXXXXXXXXXXXXXENVYHGVVSFRAYMGDPLRTMEPEDIIVTSRIMMLSSAIF 302 PNS EN Y GV+SFRA+MGDP R +E EDI VT+R+++ S +F Sbjct: 246 PNSGFPESAVAGALGIRLGGENSYQGVISFRAFMGDPDRPLEAEDIRVTNRLLVGVSVLF 305 Query: 303 VCL 305 L Sbjct: 306 TML 308 >C0Z7H8.1 C0Z7H8_BREBN Cobalamin biosynthesis protein Length = 316 Score = 269 bits (687), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 143/293 (48%), Positives = 179/293 (61%), Gaps = 3/293 (1%) Query: 6 ILLAAYVLDRIIGDPRSIPHPVVLMGKAVTAIERLIRRFCSQPRXXXXXXXXXXXXXXXX 65 +L AAY++DR++GDPR +PHPV++MG +T +E++IR + Sbjct: 6 VLCAAYLIDRVVGDPRGLPHPVIIMGWWITRLEQVIRSLVKEESHLKLAGVLFPVVIVGG 65 Query: 66 XXXXTAVILLLLSRIAPWLAWAAEVWLISTTIASKGLKDAGMAVYAELRKGDIPAARTAL 125 +IL + I P LAW WLISTTIA+KGL DAGM + L GD+ AAR +L Sbjct: 66 SYAVVWLILWGATFIHPVLAWILGAWLISTTIATKGLADAGMEIARHLVAGDMEAARRSL 125 Query: 126 GMIVGRDTTKLESPDIVRGTVETVAENIVDAIISXXXXXXXXXXXXXXXYRAVNTLDSMV 185 M+VGRDT +L+ P++ RG VETVAENIVDAI+S YRA NTLDSMV Sbjct: 126 SMVVGRDTEQLDEPEVCRGAVETVAENIVDAIVSPLIFAAIGGAPLAMAYRAANTLDSMV 185 Query: 186 GYKNDKYRNLGWASARLDDVANYIPARMTAPLLVLCAWLLRLDWRSSWRIVRRDARLHPS 245 GYKN+KY NLGWASAR+DDV NYIPAR+TA LLV +WL RLD + W ++RRDA LHPS Sbjct: 186 GYKNEKYLNLGWASARIDDVLNYIPARLTALLLVTASWLQRLDGKQCWAMIRRDAHLHPS 245 Query: 246 PNS---XXXXXXXXXXXXXXENVYHGVVSFRAYMGDPLRTMEPEDIIVTSRIM 295 PNS N Y GV S RA MGD R ++ DI+ T R+M Sbjct: 246 PNSGLPEAGVAGALGVQLGGLNYYQGVASNRAKMGDAKRPLQASDIVATIRLM 298 >A5D2P5.1 A5D2P5_PELTS Cobalamin biosynthesis protein CobD/CbiB Length = 321 Score = 238 bits (608), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 131/312 (41%), Positives = 179/312 (57%), Gaps = 3/312 (0%) Query: 4 AVILLAAYVLDRIIGDPRSIPHPVVLMGKAVTAIERLIRRFCSQPRXXXXXXXXXXXXXX 63 A+ + AY++D +GDPR +PHPVV+MGK +T +E+L RRF P Sbjct: 6 AIQVFLAYLVDLAVGDPRWLPHPVVVMGKIITFLEKLTRRFLKSPLALLAAGMLMAVALP 65 Query: 64 XXXXXXTAVILLLLSRIAPWLAWAAEVWLISTTIASKGLKDAGMAVYAELRKGDIPAART 123 T+++L + W + +WL+STTIA +GL A M V++ L KGD+ AR Sbjct: 66 AGSWYFTSLLLDWAFNLNRWAGYFLSIWLMSTTIAGRGLAGAAMKVHSLLNKGDLAGARR 125 Query: 124 ALGMIVGRDTTKLESPDIVRGTVETVAENIVDAIISXXXXXXXXXXXXXXXYRAVNTLDS 183 +GMIVGRDT +++ ++ R VETVAEN+VDA+++ YR VNTLDS Sbjct: 126 EVGMIVGRDTDRMDKKEVTRAAVETVAENVVDAVVAPVFYAFLGGAPLAMAYRTVNTLDS 185 Query: 184 MVGYKNDKYRNLGWASARLDDVANYIPARMTAPLLVLCAWLLRLDWRSSWRIVRRDARLH 243 MVGYKN+KY N G ASARLDD+AN+IPAR+T LV+ A +LR+ + ++ + RDA H Sbjct: 186 MVGYKNEKYINFGMASARLDDLANFIPARITGAALVIAALILRMSPKRTFMSILRDAPAH 245 Query: 244 PSPNS---XXXXXXXXXXXXXXENVYHGVVSFRAYMGDPLRTMEPEDIIVTSRIMMLSSA 300 PSPNS N Y+G SFR YMGD + +EP I T +M L+SA Sbjct: 246 PSPNSGIPESAVAGALGVRLGGINYYNGRESFRPYMGDEVVRLEPYHIKQTVGLMRLTSA 305 Query: 301 IFVCLCAAAAML 312 + V L A L Sbjct: 306 MVVVLGVIIAAL 317 >C1TIH3.1 C1TIH3_9FIRM Adenosylcobinamide-phosphate synthase EC=6.3.1.10 Length = 336 Score = 217 bits (552), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 125/300 (41%), Positives = 170/300 (56%), Gaps = 3/300 (1%) Query: 7 LLAAYVLDRIIGDPRSIPHPVVLMGKAVTAIERLIRRFCSQPRXXXXXXXXXXXXXXXXX 66 ++AAY LD +IGDPR + HPVV++GK ++ +E + R C P Sbjct: 21 VVAAYFLDLLIGDPRWLTHPVVIIGKLISWLEAVFFRLCKSPAALRLAGLLLAVTVVAAA 80 Query: 67 XXXTAVILLLLSRIAPWLAWAAEVWLISTTIASKGLKDAGMAVYAELRKGDIPAARTALG 126 T +I+ + I P L A E+WL+STT+A+K L AG +Y L G++ AR +G Sbjct: 81 YSVTRLIVEACTGINPLLGQAVEIWLLSTTLAAKSLAGAGREIYRLLLDGNLQEARVKVG 140 Query: 127 MIVGRDTTKLESPDIVRGTVETVAENIVDAIISXXXXXXXXXXXXXXXYRAVNTLDSMVG 186 IVGRDT KL+ + R TVETVAENIVD ++S Y+AVNTLDSMVG Sbjct: 141 WIVGRDTAKLDQSGVTRATVETVAENIVDGVLSPLFYAFLGGAPLAMAYKAVNTLDSMVG 200 Query: 187 YKNDKYRNLGWASARLDDVANYIPARMTAPLLVLCAWLLRLDWRSSWRIVRRDARLHPSP 246 YKN+KY +LGWASARLDD+AN++PAR LL+ + LL + ++ ++ A HPSP Sbjct: 201 YKNEKYLHLGWASARLDDLANFLPARWAGALLLSASALLGYNSGQAYHTIKTYAAGHPSP 260 Query: 247 NS---XXXXXXXXXXXXXXENVYHGVVSFRAYMGDPLRTMEPEDIIVTSRIMMLSSAIFV 303 NS N YHG VS RAYMG+ L +E + I T ++M +SA+ V Sbjct: 261 NSGIPESAVAGALGVRLGGLNSYHGQVSLRAYMGEELVPLEAKHIAETIKLMYAASALGV 320 >B7B1T7.1 B7B1T7_9FIRM Putative uncharacterized protein Length = 316 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 120/307 (39%), Positives = 166/307 (54%), Gaps = 4/307 (1%) Query: 6 ILLAAYVLDRIIGDPRSIPHPVVLMGKAVTAIERLIRRFCSQPRXXXXXXXXXXXXXXXX 65 I + A+++D IIGDPRS HPVVL+GK ++ +ER+ R + Sbjct: 3 IAIFAFIIDTIIGDPRSRFHPVVLIGKLISFLERIYYREEDSDQKKFFCGAILVLLVLFI 62 Query: 66 XXXXTAVILLLLSRIA-PWLAWAAEVWLISTTIASKGLKDAGMAVYAELRKGDIPAARTA 124 A I+ I W A E L++ TI+ K L AG +YA L ++ AR A Sbjct: 63 TYDVAAGIIYFSYSIPWEWGTMAVEALLLAFTISPKSLAKAGRGIYALLLTNELDKARKA 122 Query: 125 LGMIVGRDTTKLESPDIVRGTVETVAENIVDAIISXXXXXXXXXXXXXXXYRAVNTLDSM 184 +G IVGRDT KL+ ++ R TVET+AEN VD +I+ YRA NT+DSM Sbjct: 123 VGWIVGRDTAKLDESEVARATVETIAENTVDGVIAPLFFFMLGGVPLAVLYRAANTMDSM 182 Query: 185 VGYKNDKYRNLGWASARLDDVANYIPARMTAPLLVLCAWLLRLDWRSSWRIVRRDARLHP 244 +GYKN+KY G A+A+LDDV NYIPAR+TA L ++ AWLL D R++ R++RRDA HP Sbjct: 183 IGYKNEKYLYFGKAAAKLDDVLNYIPARITAVLFIIAAWLLGFDSRNALRMMRRDAAKHP 242 Query: 245 SPNSXXXXXXXXXXXXXXE---NVYHGVVSFRAYMGDPLRTMEPEDIIVTSRIMMLSSAI 301 SPN N Y G +FRAYMG+ + P+ I+ R+M ++ + Sbjct: 243 SPNGGYAEATVAGALHIRLGGINSYFGKKTFRAYMGEAIERTAPKHIMEAIRMMYTATVL 302 Query: 302 FVCLCAA 308 F+ A Sbjct: 303 FLIFVYA 309 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,535,325,242 Number of extensions: 49735275 Number of successful extensions: 159788 Number of sequences better than 1.0e-05: 687 Number of HSP's gapped: 158217 Number of HSP's successfully gapped: 688 Length of query: 318 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 183 Effective length of database: 1,788,859,178 Effective search space: 327361229574 Effective search space used: 327361229574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)