BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0004.p (333 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJX0.1 B3KJX0_9BACI D-alanine--D-alanine ligase EC=6.3.2.4 394 e-108 B7H5P5.1 B7H5P5_BACC4 D-alanine--D-alanine ligase EC=6.3.2.4 384 e-105 C2RNJ7.1 C2RNJ7_BACCE D-alanine--D-alanine ligase 384 e-105 C3I126.1 C3I126_BACTU D-alanine--D-alanine ligase 384 e-105 C2UE93.1 C2UE93_BACCE D-alanine--D-alanine ligase 384 e-105 >B3KJX0.1 B3KJX0_9BACI D-alanine--D-alanine ligase EC=6.3.2.4 Length = 305 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 189/300 (63%), Positives = 245/300 (81%), Gaps = 1/300 (0%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDFALLALH 62 KVGVIMGGVSSE EVSL TG+EM+ HLD+S+YE P+ IH + EL+ +G+D ALLALH Sbjct: 2 KVGVIMGGVSSEREVSLMTGKEMIAHLDKSRYEVYPIEIHDKRELISKTEGIDIALLALH 61 Query: 63 GAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRMEDL 122 GA+GEDGTVQGTL+TLGIPY+GSGVLASSLCMDK LSK ++R +G+HT DW+ + ME+L Sbjct: 62 GAFGEDGTVQGTLDTLGIPYTGSGVLASSLCMDKDLSKKLLRYEGLHTADWIMVSCMEEL 121 Query: 123 SPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEEYTEGREIT 182 + VE LG+PV+VKPN+GGSSIG V SA L SAVE+A D V+IE++ EG EIT Sbjct: 122 AAADVEGLGFPVVVKPNAGGSSIGTRIVRDSAGLASAVEEALRWDRFVMIEQWIEGEEIT 181 Query: 183 CPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAAALACYQALK 242 CP+L G +LP++ I+ +++FDY++KYE G A+E+VI+LP EL+E+V AAL CY+A+K Sbjct: 182 CPVLNGDMLPIVSIKP-NSEFFDYASKYEEGEADEQVIELPTELQEQVRTAALQCYKAMK 240 Query: 243 CTVYARVDMLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGLLDEIISGSLAERR 302 C+VYAR+D+++K IPY+LEVNTLPG+T+TSLLPRSA AAGY+FSGLLD +I SL+ER+ Sbjct: 241 CSVYARIDIILKGKIPYILEVNTLPGLTKTSLLPRSAEAAGYSFSGLLDALIETSLSERK 300 >B7H5P5.1 B7H5P5_BACC4 D-alanine--D-alanine ligase EC=6.3.2.4 Length = 304 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 182/300 (60%), Positives = 241/300 (80%), Gaps = 1/300 (0%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDFALLALH 62 K+GVIMGGVSSE +VS+ TG EM+ HLD++KYE VP+ ++ + +LV+ K +DFALLALH Sbjct: 2 KIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKIDLVEKAKDIDFALLALH 61 Query: 63 GAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRMEDL 122 G YGEDGTVQGTLE+LGIPYSGS +L+SS+CMDK++SK I+R +GV TPDW+ L +MEDL Sbjct: 62 GKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRYEGVETPDWIELTKMEDL 121 Query: 123 SPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEEYTEGREIT 182 + + ++ LG+P++VKPNSGGSS+G+ V + EL S +E F D V+IE+Y +G EIT Sbjct: 122 NLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVFEWDSEVVIEKYIKGDEIT 181 Query: 183 CPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAAALACYQALK 242 C IL G LP++ IR A++FDY+AKY+ E VI+LPAE++ERV+ A+LACY+ALK Sbjct: 182 CSILDGKQLPIVSIRH-AAEFFDYNAKYDDTSTVEEVIELPAEIKERVNKASLACYKALK 240 Query: 243 CTVYARVDMLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGLLDEIISGSLAERR 302 C+VYARVDM+VKDGIPYV+E+NTLPGMT++SLLP+SA AAG ++S LLD II SL R+ Sbjct: 241 CSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAGISYSKLLDMIIETSLKVRK 300 >C2RNJ7.1 C2RNJ7_BACCE D-alanine--D-alanine ligase Length = 304 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 182/300 (60%), Positives = 241/300 (80%), Gaps = 1/300 (0%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDFALLALH 62 K+GVIMGGVSSE +VS+ TG EM+ HLD++KYE VP+ ++ + +LV+ K +DFALLALH Sbjct: 2 KIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKIDLVEKAKDIDFALLALH 61 Query: 63 GAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRMEDL 122 G YGEDGTVQGTLE+LGIPYSGS +L+SS+CMDK++SK I+R +GV TPDW+ L +MEDL Sbjct: 62 GKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRYEGVETPDWIELTKMEDL 121 Query: 123 SPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEEYTEGREIT 182 + + ++ LG+P++VKPNSGGSS+G+ V + EL S +E F D V+IE+Y +G EIT Sbjct: 122 NLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVFEWDSEVVIEKYIKGDEIT 181 Query: 183 CPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAAALACYQALK 242 C IL G LP++ IR A++FDY+AKY+ E VI+LPAE++ERV+ A+LACY+ALK Sbjct: 182 CSILDGKQLPIVSIRH-AAEFFDYNAKYDDTSTVEEVIELPAEIKERVNKASLACYKALK 240 Query: 243 CTVYARVDMLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGLLDEIISGSLAERR 302 C+VYARVDM+VKDGIPYV+E+NTLPGMT++SLLP+SA AAG ++S LLD II SL R+ Sbjct: 241 CSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAGISYSKLLDMIIETSLKVRK 300 >C3I126.1 C3I126_BACTU D-alanine--D-alanine ligase Length = 304 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/300 (60%), Positives = 240/300 (80%), Gaps = 1/300 (0%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDFALLALH 62 K+GVIMGGVSSE +VS+ TG EM+ HLD++KYE VP+ ++ + +L++ K +DFALLALH Sbjct: 2 KIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALH 61 Query: 63 GAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRMEDL 122 G YGEDGTVQGTLE+LGIPYSGS +L+SS+CMDK++SK I+R +GV TPDW+ L +MEDL Sbjct: 62 GKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRYEGVETPDWIELTKMEDL 121 Query: 123 SPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEEYTEGREIT 182 + + ++ LG+P++VKPNSGGSS+G+ V + EL S +E F D V+IE+Y +G EIT Sbjct: 122 NLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVFEWDSEVVIEKYIKGDEIT 181 Query: 183 CPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAAALACYQALK 242 C IL G LP++ IR A++FDY+AKY+ E VI+LPAE+ ERV+ A+LACY+ALK Sbjct: 182 CSILDGKQLPIVSIRH-AAEFFDYNAKYDDTSTVEEVIELPAEINERVNKASLACYKALK 240 Query: 243 CTVYARVDMLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGLLDEIISGSLAERR 302 C+VYARVDM+VKDGIPYV+E+NTLPGMT++SLLP+SA AAG ++S LLD II SL R+ Sbjct: 241 CSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAGISYSKLLDMIIETSLKVRK 300 >C2UE93.1 C2UE93_BACCE D-alanine--D-alanine ligase Length = 304 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 181/300 (60%), Positives = 241/300 (80%), Gaps = 1/300 (0%) Query: 3 KVGVIMGGVSSEYEVSLNTGREMLKHLDRSKYEGVPVVIHSREELVDAVKGLDFALLALH 62 K+GVIMGGVSSE +VS+ TG EM+ HLD++KYE VP+ ++ + +L++ K +DFALLALH Sbjct: 2 KIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALH 61 Query: 63 GAYGEDGTVQGTLETLGIPYSGSGVLASSLCMDKHLSKTIIRSKGVHTPDWLCLDRMEDL 122 G YGEDGTVQGTLE+LGIPYSGS +L+SS+CMDK++SK I+R +GV TPDW+ L +MEDL Sbjct: 62 GKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRYEGVETPDWIELTKMEDL 121 Query: 123 SPEAVEALGYPVMVKPNSGGSSIGMTKVNSSAELRSAVEKAFAADCSVLIEEYTEGREIT 182 + + ++ LG+P++VKPNSGGSS+G+ V + EL S +E F D V+IE+Y +G EIT Sbjct: 122 NLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVFEWDSEVVIEKYIKGDEIT 181 Query: 183 CPILGGTLLPVIGIRSLGADWFDYSAKYELGGAEERVIQLPAELEERVHAAALACYQALK 242 C IL G LP++ IR A++FDY+AKY+ E VI+LPAE++ERV+ A+LACY+ALK Sbjct: 182 CSILDGKQLPIVSIRH-AAEFFDYNAKYDDTSTVEEVIELPAEIKERVNKASLACYKALK 240 Query: 243 CTVYARVDMLVKDGIPYVLEVNTLPGMTETSLLPRSALAAGYTFSGLLDEIISGSLAERR 302 C+VYARVDM+VKDGIPYV+E+NTLPGMT++SLLP+SA AAG ++S LLD II SL R+ Sbjct: 241 CSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAGISYSKLLDMIIETSLKVRK 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,960,971,011 Number of extensions: 81395756 Number of successful extensions: 264687 Number of sequences better than 1.0e-05: 2335 Number of HSP's gapped: 259932 Number of HSP's successfully gapped: 2350 Length of query: 333 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 197 Effective length of database: 1,779,438,163 Effective search space: 350549318111 Effective search space used: 350549318111 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)