BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0002.p (354 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAX6.1 B3KAX6_9BACI Nicotinate-nucleotide--dimethylbenzimidazo... 361 6e-98 B1DLT8.1 B1DLT8_9BACL Nicotinate-nucleotide--dimethylbenzimidazo... 339 2e-91 Q24VN9.1 Q24VN9_DESHY Nicotinate-nucleotide--dimethylbenzimidazo... 307 9e-82 B8G2F3.1 B8G2F3_DESHD Nicotinate-nucleotide/dimethylbenzimidazol... 307 9e-82 A4J829.1 COBT_DESRM Nicotinate-nucleotide--dimethylbenzimidazole... 298 6e-79 >B3KAX6.1 B3KAX6_9BACI Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase EC=2.4.2.21 Length = 356 Score = 361 bits (926), Expect = 6e-98, Method: Compositional matrix adjust. Identities = 189/349 (54%), Positives = 233/349 (66%) Query: 2 MISAIVEVTGPIAPPDEQAALRAVLRLNSLTKPPGSLGQLEALAVRLAGISKTEQPSYGT 61 + S + +T I P D +A L++LTKPPGSLG+LE+LA++LAGI+ +PS+ Sbjct: 5 LTSRLKGITANIKPLDAEAQAETAAYLDTLTKPPGSLGKLESLAIQLAGITGKMKPSFTK 64 Query: 62 RTVVVMAADHGVCSEGVSAFPQEVTVQMAYNFLSGGAAVNVLARQAGASVQFVDIGIQAE 121 +VVVMA DHGVC EG+SAFP EVT QM NFL+GGAA+NVLARQAGA V VDIG+ E Sbjct: 65 TSVVVMAGDHGVCDEGISAFPAEVTPQMVMNFLNGGAAINVLARQAGADVHCVDIGVATE 124 Query: 122 LEHPELINRKVRPGTDNMAAGPAMSREEALEAVLVGVKVAQEAIKSGTEILITGEMGIGN 181 L H L++R + GT NMA GPAM+REEAL++V+ G +VA E +K GT++ +TGEMGIGN Sbjct: 125 LTHERLLSRNIGRGTANMAKGPAMTREEALQSVVTGAEVAAELVKQGTQMFVTGEMGIGN 184 Query: 182 TTASAAILCALGGLPPEAAVGRGTGINDERLLHKIEVVERALRVNAPNPADPVDVLSKVX 241 TT SAAI L G P VGRGTG+ DERL HKI VVERA++VNAP+ ADP+DVL+KV Sbjct: 185 TTPSAAIFSVLSGKQPSEVVGRGTGVTDERLNHKIRVVERAIQVNAPHAADPLDVLAKVG 244 Query: 242 XXXXXXXXXXXXXXXXXRVPVILDGFISGAAALVAKAIAPESTAYMIASHVSNEQGHKXX 301 PVI+DGFIS AAALVAK+I P++ YMIASHVS+EQ H Sbjct: 245 GFEIGGLAGLILGAAAHGCPVIIDGFISSAAALVAKSICPDALPYMIASHVSHEQAHVLL 304 Query: 302 XXXXXXXXXXXXXXXXXXXXXXXXSLHLIEAASRIMTEMATFESAGVSG 350 LHL+EAA RI+ EMATFESAG+SG Sbjct: 305 LKELGLQPMLQMDMRLGEGTGGVLCLHLVEAACRIVNEMATFESAGISG 353 >B1DLT8.1 B1DLT8_9BACL Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase EC=2.4.2.21 Length = 355 Score = 339 bits (870), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 183/342 (53%), Positives = 218/342 (63%) Query: 8 EVTGPIAPPDEQAALRAVLRLNSLTKPPGSLGQLEALAVRLAGISKTEQPSYGTRTVVVM 67 +V I P EQA + A + LTKP GSLG+LE +A RLAGIS + P + VVVM Sbjct: 12 QVIERIRPFQEQAVIEAKAHSDQLTKPTGSLGKLEEIAFRLAGISGSLWPDLSRKAVVVM 71 Query: 68 AADHGVCSEGVSAFPQEVTVQMAYNFLSGGAAVNVLARQAGASVQFVDIGIQAELEHPEL 127 A DHGVC EGVSA+PQEVT QM NFLSGGAAVNVLARQAGA V VDIG+ AELEHP L Sbjct: 72 AGDHGVCEEGVSAYPQEVTPQMVLNFLSGGAAVNVLARQAGADVYCVDIGVNAELEHPGL 131 Query: 128 INRKVRPGTDNMAAGPAMSREEALEAVLVGVKVAQEAIKSGTEILITGEMGIGNTTASAA 187 I+RKV GT NMA PAMSREEAL+A++ GV+VA E G + TGEMGIGNTT SAA Sbjct: 132 ISRKVVRGTANMAKQPAMSREEALQAIMAGVQVADELADKGYRLFATGEMGIGNTTPSAA 191 Query: 188 ILCALGGLPPEAAVGRGTGINDERLLHKIEVVERALRVNAPNPADPVDVLSKVXXXXXXX 247 + L GL P+ +VGRGTGINDE HKIEVV+RA+ VN P+ +DP+DVL+KV Sbjct: 192 LTTVLTGLSPDLSVGRGTGINDESWRHKIEVVKRAIAVNKPDASDPLDVLAKVGGAEIAG 251 Query: 248 XXXXXXXXXXXRVPVILDGFISGAAALVAKAIAPESTAYMIASHVSNEQGHKXXXXXXXX 307 PV++DGFIS AALVA +A ++ Y+ ASH+S EQGH Sbjct: 252 LAGFIIGAAANGCPVVVDGFISSVAALVAVRLAEQAKPYLFASHLSQEQGHAVVLESMGL 311 Query: 308 XXXXXXXXXXXXXXXXXXSLHLIEAASRIMTEMATFESAGVS 349 H ++AA ++M EMATF SAGVS Sbjct: 312 SPMIQLDMRLGEGTGAVLCFHFVDAALKLMQEMATFSSAGVS 353 >Q24VN9.1 Q24VN9_DESHY Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Length = 368 Score = 307 bits (787), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 164/334 (49%), Positives = 209/334 (62%) Query: 17 DEQAALRAVLRLNSLTKPPGSLGQLEALAVRLAGISKTEQPSYGTRTVVVMAADHGVCSE 76 DEQ RL+SLTKP GSLG LEALA +L GI P + ++VMA DHGV +E Sbjct: 33 DEQVMAAVQTRLDSLTKPQGSLGVLEALAKQLGGIQGQPWPEVQKKAILVMAGDHGVVAE 92 Query: 77 GVSAFPQEVTVQMAYNFLSGGAAVNVLARQAGASVQFVDIGIQAELEHPELINRKVRPGT 136 GVSAFPQEVT QM YNFL GGA +NVLAR AGA V D+G+ L+ PEL+ ++ GT Sbjct: 93 GVSAFPQEVTPQMFYNFLGGGAGINVLARHAGAEVICTDVGMAFPLDPPELMAHRIMNGT 152 Query: 137 DNMAAGPAMSREEALEAVLVGVKVAQEAIKSGTEILITGEMGIGNTTASAAILCALGGLP 196 NMA GPAMSR+EAL ++L G K+A++AI SG +L TGE+GIGNTT S AI+ L GLP Sbjct: 153 HNMAQGPAMSRKEALLSLLTGAKIARQAIDSGVNVLATGEVGIGNTTPSTAIISLLTGLP 212 Query: 197 PEAAVGRGTGINDERLLHKIEVVERALRVNAPNPADPVDVLSKVXXXXXXXXXXXXXXXX 256 P+ GRGTG++DE L+ K V+ R++ +N P+P D VD+LSK+ Sbjct: 213 PQEVTGRGTGLDDEGLIRKQNVIARSIAINQPDPKDGVDILSKIGGLEIGALAGAILTAA 272 Query: 257 XXRVPVILDGFISGAAALVAKAIAPESTAYMIASHVSNEQGHKXXXXXXXXXXXXXXXXX 316 RVP++LDG IS AAAL+A I P + ++I SH S E GH+ Sbjct: 273 YSRVPILLDGVISTAAALIAVRICPTARFFLIPSHSSAEIGHRAALEQLELKPIMHLDFR 332 Query: 317 XXXXXXXXXSLHLIEAASRIMTEMATFESAGVSG 350 + HL++A+ RI+ EMATFESAGVSG Sbjct: 333 LGEGTGAAIAFHLLDASIRILNEMATFESAGVSG 366 >B8G2F3.1 B8G2F3_DESHD Nicotinate-nucleotide/dimethylbenzimidazole phosphoribosyltransferase EC=2.4.2.21 Length = 368 Score = 307 bits (787), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 164/334 (49%), Positives = 209/334 (62%) Query: 17 DEQAALRAVLRLNSLTKPPGSLGQLEALAVRLAGISKTEQPSYGTRTVVVMAADHGVCSE 76 DEQ RL+SLTKP GSLG LEALA +L GI P + ++VMA DHGV +E Sbjct: 33 DEQVMAAVQTRLDSLTKPQGSLGVLEALAKQLGGIQGQPWPEVQKKAILVMAGDHGVVAE 92 Query: 77 GVSAFPQEVTVQMAYNFLSGGAAVNVLARQAGASVQFVDIGIQAELEHPELINRKVRPGT 136 GVSAFPQEVT QM YNFL GGA +NVLAR AGA V D+G+ L+ PEL+ ++ GT Sbjct: 93 GVSAFPQEVTPQMFYNFLGGGAGINVLARHAGAEVICTDVGMAFPLDPPELMAHRIMNGT 152 Query: 137 DNMAAGPAMSREEALEAVLVGVKVAQEAIKSGTEILITGEMGIGNTTASAAILCALGGLP 196 NMA GPAMSR+EAL ++L G K+A++AI SG +L TGE+GIGNTT S AI+ L GLP Sbjct: 153 HNMAQGPAMSRKEALLSLLTGAKIARQAIDSGVNVLATGEVGIGNTTPSTAIISLLTGLP 212 Query: 197 PEAAVGRGTGINDERLLHKIEVVERALRVNAPNPADPVDVLSKVXXXXXXXXXXXXXXXX 256 P+ GRGTG++DE L+ K V+ R++ +N P+P D VD+LSK+ Sbjct: 213 PQEVTGRGTGLDDEGLIRKQNVIARSIAINQPDPKDGVDILSKIGGLEIGALAGAILTAA 272 Query: 257 XXRVPVILDGFISGAAALVAKAIAPESTAYMIASHVSNEQGHKXXXXXXXXXXXXXXXXX 316 RVP++LDG IS AAAL+A I P + ++I SH S E GH+ Sbjct: 273 YSRVPILLDGVISTAAALIAVRICPTARFFLIPSHSSAEIGHRAALEQLELKPIMHLDFR 332 Query: 317 XXXXXXXXXSLHLIEAASRIMTEMATFESAGVSG 350 + HL++A+ RI+ EMATFESAGVSG Sbjct: 333 LGEGTGAAIAFHLLDASIRILNEMATFESAGVSG 366 >A4J829.1 COBT_DESRM Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase EC=2.4.2.21 Length = 351 Score = 298 bits (763), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 169/349 (48%), Positives = 215/349 (61%), Gaps = 2/349 (0%) Query: 3 ISAIVEVTGPIAPPDEQAALRAVLRLNSLTKPPGSLGQLEALAVRLAGISKTEQPSYGTR 62 ++ + E I PD+ +A RL+SLTKPPGSLG LE +A RLAGI + P Sbjct: 1 MTLLAETLSKIQAPDQTCVEQAKQRLDSLTKPPGSLGILEDIAWRLAGIQRVPLPQLPRE 60 Query: 63 TV-VVMAADHGVCSEGVSAFPQEVTVQMAYNFLSGGAAVNVLARQAGASVQFVDIGIQAE 121 V +V+A DHGV +EGVSAFPQEVT QM +NFL GGA +NVLARQAGA V DIG+ Sbjct: 61 KVSLVLAGDHGVVAEGVSAFPQEVTPQMIFNFLQGGAGINVLARQAGAKVVVADIGVAGP 120 Query: 122 -LEHPELINRKVRPGTDNMAAGPAMSREEALEAVLVGVKVAQEAIKSGTEILITGEMGIG 180 LE L++ +V+ GTDN A GPAMSREEA++++ G+ + Q+ +K ++ GEMGIG Sbjct: 121 PLEKTGLVSCRVKSGTDNFAVGPAMSREEAVKSIEAGISLLQQQVKERPALVAIGEMGIG 180 Query: 181 NTTASAAILCALGGLPPEAAVGRGTGINDERLLHKIEVVERALRVNAPNPADPVDVLSKV 240 NTT SAAIL A G+P E GRGTGI+++RL HKI V++R L VN P+ D +DVLSKV Sbjct: 181 NTTPSAAILAAFTGMPVEEITGRGTGIDNQRLQHKIAVIKRGLEVNRPDANDGLDVLSKV 240 Query: 241 XXXXXXXXXXXXXXXXXXRVPVILDGFISGAAALVAKAIAPESTAYMIASHVSNEQGHKX 300 +PV+LDGFISGAAALVA+++AP S YM ASH S E GH+ Sbjct: 241 GGLEIGGMAGIILGCAAEGIPVVLDGFISGAAALVAQSLAPLSREYMFASHGSVEPGHRI 300 Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXSLHLIEAASRIMTEMATFESAGVS 349 + +IE+A RI+ EMATF AGVS Sbjct: 301 MLEKLGLKPMLQMEMRLGEGTGAALAYPIIESAVRIINEMATFAEAGVS 349 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.130 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,685,462,323 Number of extensions: 59213672 Number of successful extensions: 171037 Number of sequences better than 1.0e-05: 736 Number of HSP's gapped: 169008 Number of HSP's successfully gapped: 739 Length of query: 354 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 217 Effective length of database: 1,770,017,148 Effective search space: 384093721116 Effective search space used: 384093721116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)