BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0517.0005.p (523 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB34.1 B1DB34_9BACL Extracellular solute-binding protein family 1 872 0.0 A9KLI6.1 A9KLI6_CLOPH Extracellular solute-binding protein family 1 197 3e-48 B1DB85.1 B1DB85_9BACL ABC-type sugar transport system periplasmi... 186 4e-45 B3KFS5.1 B3KFS5_9BACI Extracellular solute-binding protein family 1 170 4e-40 B3KF69.1 B3KF69_9BACI Extracellular solute-binding protein family 1 142 1e-31 >B1DB34.1 B1DB34_9BACL Extracellular solute-binding protein family 1 Length = 532 Score = 872 bits (2254), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/472 (87%), Positives = 447/472 (94%) Query: 51 PMEITWGIHLQAPGVVENSQVQKWLEEKFNVKIKPVKVTDASIASGEVPDIFMLGDPANV 110 P+EITWGIH QA GVVEN++VQKWLE+KFNVKIKPVKVTDASIASG+VPDIFMLGDPANV Sbjct: 60 PIEITWGIHFQAAGVVENTEVQKWLEQKFNVKIKPVKVTDASIASGDVPDIFMLGDPANV 119 Query: 111 TAYQNQGVLMNLDQNMLKEKMPEYYADIEKNNALFQTVTIDDKLWAIPMFIDLKPYDLGM 170 +AYQNQGVLM++D N LKEKMPEYYADIEK + LFQTVT +D+LWAIPMFIDLKPYDLGM Sbjct: 120 SAYQNQGVLMSIDPNQLKEKMPEYYADIEKKSKLFQTVTFNDQLWAIPMFIDLKPYDLGM 179 Query: 171 LWRKDWLDKVGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTATSTWSSGFYSI 230 LWRKDWL+ VGITK PETLDEFE+A+YAFAQKDPD NG+KDTYGLTGTATSTWSSGFYSI Sbjct: 180 LWRKDWLNNVGITKVPETLDEFEQAVYAFAQKDPDKNGKKDTYGLTGTATSTWSSGFYSI 239 Query: 231 FGAFGVEPTMWHEKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLY 290 FGAFGVEPTMWHEKNGEI+NGSVMPETKEALAKLRQW+ADGVIDPEFITDTQDSYRKKLY Sbjct: 240 FGAFGVEPTMWHEKNGEIVNGSVMPETKEALAKLRQWYADGVIDPEFITDTQDSYRKKLY 299 Query: 291 NNRIGVIEEQIAKGALPDAATVTEMKSLNPSAELVFTKNPKGPGGDGSWDWGVKSNFIVI 350 NNRIGVIEEQIAKGALP+ TVTEMKSL+P+AELVF+KNPKGPGGDGSWDWGVKSNF+VI Sbjct: 300 NNRIGVIEEQIAKGALPEGGTVTEMKSLSPNAELVFSKNPKGPGGDGSWDWGVKSNFVVI 359 Query: 351 SNKVKDQPEKLNKLFEILRAESTDEETINMTSLGVKGTHWDFTESGATSGATQFKAGFEK 410 +KVKDQPEKL+KL+EILRAES+DEETINMTSLGVKGTHWDF ESGA SGAT+F FEK Sbjct: 360 GSKVKDQPEKLDKLYEILRAESSDEETINMTSLGVKGTHWDFAESGAASGATKFLPSFEK 419 Query: 411 AEERDQAGIRLFSLGNITTQAYREKYSNPALNEAIKTYSSAPRQTDALLFSALPSDGKYK 470 E+RDQ GIRLFSLGNITTQAYREKYSNP LNEAIKTYSSAP TDALLFSALPSDGKYK Sbjct: 420 QEQRDQEGIRLFSLGNITTQAYREKYSNPKLNEAIKTYSSAPNLTDALLFSALPSDGKYK 479 Query: 471 NDLTSLMQKYFAQIISGEIPLEDFDKFVAEWKSRGGDELTKEANEMYQAQFK 522 DLT+LMQKYFAQIISGEIPL+DFDKFV EWK+RGGDELTKEANE+YQAQFK Sbjct: 480 ADLTALMQKYFAQIISGEIPLDDFDKFVTEWKARGGDELTKEANELYQAQFK 531 >A9KLI6.1 A9KLI6_CLOPH Extracellular solute-binding protein family 1 Length = 544 Score = 197 bits (500), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 150/513 (29%), Positives = 238/513 (46%), Gaps = 76/513 (14%) Query: 51 PMEITWGIHLQAPGVVENSQVQKWLEEKFNVKIKPVKVTD--------ASIASGEVPDIF 102 P+ I W + ++++ K +E+KFNVK + V D A ++SG++PD+ Sbjct: 53 PVTIEWLAYNTYSQPNTDTEIVKQIEKKFNVKFEFWYVDDQKWDEILGAKLSSGDMPDVM 112 Query: 103 MLGDPANVTAYQNQGVLMNLDQNMLKEKMPEYYADIEKNNALFQTVT---IDDKLWAIPM 159 + + AN+ Y QG+L ML K+P + +E+ N + D K +AI Sbjct: 113 KIKNTANIPTYVKQGILAEFTDEMLA-KIPSFTKQVEEANVEGNGLIDAYYDGKRYAIKT 171 Query: 160 FIDLKPYDLGMLWRKDWLDKVGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTA 219 Y ++WR DWL +GI K P T+DE E+A+YA DPDGNG KDTYG++ TA Sbjct: 172 PSISGTYPTVLVWRTDWLKNLGIEKIPATIDEMEEAMYAIRNNDPDGNGVKDTYGMSNTA 231 Query: 220 TSTWSSGFYSIFGAFGVEP-----------TMWHEKNGEIMNGSVMPETKEALAKLRQWF 268 + ++FGA+G P + EK+G+I PE K ALA +++W+ Sbjct: 232 MN-------AVFGAYGAIPLKEFRGTGAQNLFFTEKDGKIEFACTQPEMKAALATIQKWY 284 Query: 269 ADGVIDPEFITDTQD----SYRKKLYNNRIGVIEEQIAKGALP-------DAATVTEMKS 317 +G+IDPEFIT + + N ++GV +A P A + Sbjct: 285 KEGLIDPEFITGENTAGYWATSQAFENGKVGVTGMALASHWAPPVEEGKKGGACYEGFVA 344 Query: 318 LNPSAELVFTKN-------PKGPGGDGSWDWGVKSNFIVISNKVKDQPEKLNKLFEILRA 370 +NP A+ T N P+G G +W S F I+ K + P K++ + ++ A Sbjct: 345 MNPDAKWEETVNIGPAIQGPEGKSGTHTWGAFSPSGF-GITTKCAEDPRKVDAILAMIEA 403 Query: 371 ESTDEETINMTSLGVKGTHWDFTESGATSGATQFKAGFEKAEERDQAGIRLFSLGNIT-- 428 S+D E + G++GTH++ TE G G + + F K E Q G+ +F LG T Sbjct: 404 YSSDPEYALLAGWGIEGTHYEKTEEG---GVRRLEP-FTKPSEYIQDGVGVFMLGTNTEF 459 Query: 429 -------TQAYREKYSNPALNEAIKTYSSAPRQTDALLFSALPSDGKYKNDLTSLMQKYF 481 + +KY P + +L A + +Y DL + Sbjct: 460 DRSLSKNVFDFSDKYKTPGYQD--------------ILVPATEAANQYLTDLKIFTLDAY 505 Query: 482 AQIISGEIPLEDFDKFVAEWKSRGGDELTKEAN 514 +I++GE ++ FD FV E+ S GG+++ E N Sbjct: 506 IKIMTGEESVDYFDTFVKEFNSMGGEQILNEIN 538 >B1DB85.1 B1DB85_9BACL ABC-type sugar transport system periplasmic component-like protein Length = 529 Score = 186 bits (473), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 140/486 (28%), Positives = 244/486 (50%), Gaps = 31/486 (6%) Query: 49 EAPMEITWGIHLQAPGVVENSQVQKWLEEKFNVKIKPVKVTDAS----IASGEVPDIF-M 103 E+P+EI+ G P + + QK+LEEK+NVK+ + +TD S +AS E+PDIF Sbjct: 52 ESPIEISIGSTWNIPSA-DGNPTQKYLEEKYNVKLINMNLTDESFKLKLASNEIPDIFPG 110 Query: 104 LGDPANVTAYQNQGVLMNLDQNMLKEKMPEYYADIEK-NNALFQTVTIDDKLWAIPMFID 162 A++ Y QG++ ++ + +K+ MP+Y ADI++ + A + +D K + +P Sbjct: 111 TLQEADMANYAKQGIIASISTDEIKQYMPKYSADIDQVDTAAWGIAMVDGKNYGVPRIWL 170 Query: 163 LKPYDLGMLWRKDWLDKVGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTATST 222 Y + WL+K+G T+AP+TL+E+E + F DPDGNG+KDTYG+TG S Sbjct: 171 NGRYGFIPTYNGAWLNKIGYTEAPKTLEEYEDVLTKFRNDDPDGNGKKDTYGMTGRGKSA 230 Query: 223 WSSGFYSIFGAFGVEPTMWH-EKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDT 281 + F +F A+GV P + +G + G + + KEA+ K+ QW+ +IDPEF+TD Sbjct: 231 RNQLFNEVFAAYGVNPYQFMAAADGTVTWGGITDQAKEAVKKINQWYKSDLIDPEFLTDD 290 Query: 282 QDSYRKKLYNNRIGVIEEQIAKGALPDAATVTEMKSLNPSAELVFTKNPKGPGGDGSWDW 341 QD K + +IG+ + + E N + + K GP G Sbjct: 291 QDQINTKWNSQKIGIKDNYMYHHLFGQ----LEQDKAN-GFPVAYGKGLIGPAGKSY--- 342 Query: 342 GVKSNF-----IVISNKVKDQPEKLNKLFEILRAESTDEETINMTSLGVKGTHWDFTESG 396 V SN ++ +++ +K K+ ++L +T++E T+ G +G +D + Sbjct: 343 -VMSNGPLQTPLMFGKQLEKDDKKRIKILQMLEDLATNDEVYLRTTYGEEGKSYDLVD-- 399 Query: 397 ATSGATQFKAGF-EKAEERDQAGIRLFSLGNITTQAYREKYSNPALNEAI--KTYSSAPR 453 G +K + + + ++ G F + K+ PA +A K + + Sbjct: 400 ---GVPVWKKEYLDTPQLQNDLGTGGFYNPFVDRSLSNAKFQMPADKQAFSDKLNAGNEK 456 Query: 454 QTDALLFSALPSDGKYKNDLTSLMQKYFAQIISGEIPLE-DFDKFVAEWKSRGGDELTKE 512 +D L + L S KY +L +L ++++++ + +E +F+ F +EW GG E+ E Sbjct: 457 ISDLLGPTPLESKTKYLANLNTLEDSFYSKVMVTKGDVEKEFESFKSEWLKAGGQEMLDE 516 Query: 513 ANEMYQ 518 A ++YQ Sbjct: 517 ATKVYQ 522 >B3KFS5.1 B3KFS5_9BACI Extracellular solute-binding protein family 1 Length = 519 Score = 170 bits (430), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 144/473 (30%), Positives = 229/473 (48%), Gaps = 68/473 (14%) Query: 74 WLEEK----FNVKIKPVKV----TDASIASGEVPDIFMLGDPANVTAYQNQGVLMNLDQN 125 ++EE+ V + P +V D ++SG +PD+ +P Y Q M LD+ Sbjct: 89 YIEERTGLEIGVNLPPPEVYNEKLDVIMSSGNLPDMLHTYEPVWFDNYVKQKAFMPLDEL 148 Query: 126 M------LKEKMPEYYADIEKNNALFQTVTIDDKLWAIPMFIDLKPYDLGMLWRKDWLDK 179 + LKEK+P ++ V K++A+P ++ +L M RKDWLD+ Sbjct: 149 IDEYGPHLKEKIPP---------EVWDRVRYGGKIYAVPSLNEVTGAEL-MYVRKDWLDR 198 Query: 180 VGITKAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTATSTWSSGFYSIFGAFGVEPT 239 +G+ + PETL+E+ + I AF DPDGNG +DT GLT T++ SS F FGAFG + Sbjct: 199 LGL-QPPETLEEYYEVIRAFTLDDPDGNGVQDTIGLTLTSSLGRSSPF---FGAFGTQRN 254 Query: 240 MWHEKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLYNNRIGVIEE 299 W E++G+++NGS++PETKEALA + + + ++D EF + Q++ +K+ N +G+ Sbjct: 255 TWFERDGQLVNGSILPETKEALAFFAKLYQENLLDREFPLNLQNNLFEKIENGTVGLFSA 314 Query: 300 QIAKGALPDAATVTEMKSLNPSAELVFTKNPKGPGGDGSWDWGVKSNFIVIS-NKVKDQP 358 P AA +++ +P AE + P GP G GV+ I+ N + + Sbjct: 315 AWYDTRGPIAANMSK----DPEAEWMPLPYPTGPDGFK----GVEGKDIIRGYNVIPAES 366 Query: 359 EKLNKLFEILRAESTDEETINMTSLGVKGTHWDFTESGATSGATQFKAGFEKAEERDQAG 418 EK ++ L ++D +T+ LG + W + + + + + Sbjct: 367 EKAAEVIRFLDFIASDYKTL---KLGFENEIWRWEDGAIVTDFALHDKHLYRGIYQSLVD 423 Query: 419 I--------RLFSLGNITTQAYREKYSNPAL-NEAIKTYSSAPRQTDALLFSALPSDG-- 467 + RL SLG+ Y+N L N+ I + F +P+ G Sbjct: 424 VPDQLLFKERLDSLGDFNL------YNNLQLINQHIIPNA----------FYGVPTPGMT 467 Query: 468 KYKNDLTSLMQKYFAQIISGEIPLEDFDKFVAEWKSRGGDELTKEANEMYQAQ 520 KY L +L + F +II G PL+ FD +V WK GGDE+TKE NE Y Q Sbjct: 468 KYNTKLKNL-EDVFTKIIMGLEPLDAFDDYVKTWKEEGGDEITKEVNEWYSKQ 519 >B3KF69.1 B3KF69_9BACI Extracellular solute-binding protein family 1 Length = 383 Score = 142 bits (357), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 97/308 (31%), Positives = 168/308 (54%), Gaps = 24/308 (7%) Query: 70 QVQKWLEEKFNVKIKPV-----KVTDASIASGEVPDIFMLGDPANVTAYQNQGVLMNLDQ 124 ++ K L N+ + P + ++SG PD+FM+ D + + QG+L++L Sbjct: 75 ELDKALNIDLNLTVYPAADDYNNQLNVRMSSGNFPDLFMV-DRQQLIQFSKQGLLLDLTP 133 Query: 125 NMLKEKMPEYYADIEKNNALFQTVTIDDKLWAIPMFIDLKPYDLGMLW-RKDWLDKVGIT 183 M +K+ + A I +++ + +D K++ I + PY+ LW RKDWLDK+ + Sbjct: 134 YM--DKLEQTVAFIGEDSV--KKGMVDGKVYGISKSPQI-PYN--TLWIRKDWLDKLKL- 185 Query: 184 KAPETLDEFEKAIYAFAQKDPDGNGQKDTYGLTGTATSTWSSGFYSIFGAFGVE-PTMWH 242 + P T+++ AF + DPDGNG+ DT+GLTG+ S +S ++FGAFGV P ++ Sbjct: 186 EVPTTVEQLASVAEAFTKNDPDGNGKPDTFGLTGSKLSAFS----TVFGAFGVGMPGNFY 241 Query: 243 EKNGEIMNGSVMPETKEALAKLRQWFADGVIDPEFITDTQDSYRKKLYNNRIGVIEEQIA 302 EK+G+++N P K+ALA ++ G +DPE + ++ +++K + G++ I Sbjct: 242 EKDGKVVNSLHEPAMKDALAFIKDMIGSGSVDPELLANSGLQHQEKAIKGQAGIV--YID 299 Query: 303 KGALPDAATVTEMKSLNPSAELVFTKNPKGPGG--DGSWDWGVKSNFIVISNKVKDQPEK 360 + V ++K++NP+AE + P G GG DGSWD G I ++ PEK Sbjct: 300 WPNMTKEQFVEQIKTVNPNAEWLQIAAPSGTGGQFDGSWDIGAAPGRYAIPKALEKDPEK 359 Query: 361 LNKLFEIL 368 L ++F++L Sbjct: 360 LQRVFDLL 367 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,119,736,457 Number of extensions: 136383066 Number of successful extensions: 268627 Number of sequences better than 1.0e-05: 221 Number of HSP's gapped: 268423 Number of HSP's successfully gapped: 222 Length of query: 523 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 383 Effective length of database: 1,741,754,103 Effective search space: 667091821449 Effective search space used: 667091821449 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)