BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0517.0003.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB36.1 B1DB36_9BACL Binding-protein-dependent transport system... 484 e-135 B1DB84.1 B1DB84_9BACL Binding-protein-dependent transport system... 244 6e-63 B3KF68.1 B3KF68_9BACI Binding-protein-dependent transport system... 239 2e-61 A9KLI5.1 A9KLI5_CLOPH Binding-protein-dependent transport system... 228 5e-58 B3K469.1 B3K469_9BACI Binding-protein-dependent transport system... 213 1e-53 >B1DB36.1 B1DB36_9BACL Binding-protein-dependent transport systems inner membrane component Length = 289 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 236/289 (81%), Positives = 260/289 (89%) Query: 1 MKTRKWQDLITPAIIYLVLSLLALIVVYPFIHVTAVSFSGRGEALRSGFHFFPRDFEWRA 60 MK +WQ+ I P +IY VL+ LA+IVVYPFIHV AVSFSGRGEALRSGFHFFPRD +W A Sbjct: 1 MKATRWQERIAPGLIYFVLASLAVIVVYPFIHVIAVSFSGRGEALRSGFHFFPRDIQWTA 60 Query: 61 YRELLEYPFIWSGYANTVFRMXXXXXXXXXXXXCAAYPLSRPDFPMKRVLIMLILFTMIF 120 YRELL YPFIWSGY+NT+FRM CAAYPLSRPDFP+KR+L +L+LFTMIF Sbjct: 61 YRELLNYPFIWSGYSNTIFRMVVGTALTLLVTVCAAYPLSRPDFPVKRLLTLLLLFTMIF 120 Query: 121 EGGIVPNYLLIKELHLLNSRWVYVLPTAANAFQVLVMLSFFRSIPDALIEAARIDGARDM 180 +GGIVP+YLL+K+LHLLNS WVYVLPTAANAFQVLVMLSFFRS+PDALIEAARIDGARD+ Sbjct: 121 DGGIVPHYLLMKDLHLLNSLWVYVLPTAANAFQVLVMLSFFRSLPDALIEAARIDGARDI 180 Query: 181 RILFRIVLPLSIPSLVTIGLWSLVHHINAFMDNLLYVTDQSKYVLQQVVRQILIENKLDP 240 ILFRIVLPLSIP LVTIGLWS+VHHINAFMDNLLYVTDQSK+VLQQVVRQILIENK+D Sbjct: 181 GILFRIVLPLSIPPLVTIGLWSMVHHINAFMDNLLYVTDQSKFVLQQVVRQILIENKVDA 240 Query: 241 FTTATNLTPPAPESLKMASVIVCSLPVLVLYPFLLKYFEKGTMIGSVKG 289 FTTATN+ PPAPESLKMASVIVC+LPVLV+YPFLLKYFEKGTMIGSVKG Sbjct: 241 FTTATNMIPPAPESLKMASVIVCTLPVLVVYPFLLKYFEKGTMIGSVKG 289 >B1DB84.1 B1DB84_9BACL Binding-protein-dependent transport systems inner membrane component Length = 290 Score = 244 bits (624), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 127/271 (46%), Positives = 173/271 (63%), Gaps = 6/271 (2%) Query: 23 ALIVVYPFIHVTAVSFSGRGEALRSGFHFFPRDFEWRAYRELLEYPFIWSGYANTVFRMX 82 +I +YPFIH+ +VSFS EA R GFH+FPRD + ++++LL IW G NT R Sbjct: 22 CVIAIYPFIHIASVSFSAPKEAGRFGFHWFPRDVDLFSWKQLLTQKKIWIGMRNTTLRTV 81 Query: 83 XXXXXXXXXXXCAAYPLSRPDFPMKRVLIMLILFTMIFEGGIVPNYLLIKELHLLNSRWV 142 AAYPLSR P + M+I+FTM F GG++P YLLIK+LHLLN+ WV Sbjct: 82 LGTLLTLIAMALAAYPLSRKHLPHRSFYTMIIVFTMFFSGGLIPTYLLIKDLHLLNNFWV 141 Query: 143 YVLPTAANAFQVLVMLSFFRSIPDALIEAARIDGARDMRILFRIVLPLSIPSLVTIGLWS 202 Y+LP F +L+M +FF +IP + E+A+IDGA DMRIL+ IVLPLS P L T+GLW+ Sbjct: 142 YILPGLIPTFSMLIMRNFFMTIPVEVEESAKIDGANDMRILWSIVLPLSKPVLATLGLWA 201 Query: 203 LVHHINAFMDNLLYVTDQSKYVLQQVVRQILIENK----LDPFTTATNLTPPAPESLKMA 258 V + NA+ D ++YVTD +Q +R++++ + T A NL S+K A Sbjct: 202 AVANWNAWFDAIIYVTDTDLKTIQFFLREVVVTSADAEIFGRVTGAANLQ--YETSIKAA 259 Query: 259 SVIVCSLPVLVLYPFLLKYFEKGTMIGSVKG 289 +VI +P+LV+YPFL KYF KGTM+GS+KG Sbjct: 260 TVIFAVVPILVVYPFLQKYFVKGTMVGSLKG 290 >B3KF68.1 B3KF68_9BACI Binding-protein-dependent transport systems inner membrane component Length = 286 Score = 239 bits (611), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 123/287 (42%), Positives = 186/287 (64%), Gaps = 4/287 (1%) Query: 4 RKWQDLITPAIIYLVLSLLALIVVYPFIHVTAVSFSGRGEALRSG-FHFFPRDFEWRAYR 62 R D I AIIY++L + L+ + P ++V +VS + GE LR+G F FP++ + AYR Sbjct: 3 RGLDDKIFNAIIYVILGICGLVAILPILYVVSVSITPFGEVLRNGGFILFPKEITFSAYR 62 Query: 63 ELLEYPFIWSGYANTVFRMXXXXXXXXXXXXCAAYPLSRPDFPMKRVLIMLILFTMIFEG 122 LL I + TV AYPLSR + P + +++I+FT++F G Sbjct: 63 TLLTESNIPKAFQITVLITVIGTAVNLVLTGLMAYPLSRRNLPGRNFFLLMIVFTLLFSG 122 Query: 123 GIVPNYLLIKELHLLNSRWVYVLPTAANAFQVLVMLSFFRSIPDALIEAARIDGARDMRI 182 G++P YL++K + LL+S W +LP A +F VL+M SFF +P+ L E+AR+DGA++ RI Sbjct: 123 GLIPTYLVVKSMGLLDSIWAMILPNAVWSFNVLIMKSFFEGLPEELFESARMDGAKEFRI 182 Query: 183 LFRIVLPLSIPSLVTIGLWSLVHHINAFMDNLLYVTDQSKYVLQQVVRQILIENKLDPFT 242 L +IV PLS+P L+T+GL+ LV H N F + YVTD++ Y LQ VVR+IL++++ P Sbjct: 183 LLQIVTPLSVPVLLTVGLFYLVGHWNEFFQAIFYVTDRTLYPLQVVVREILMQSQ-QPLE 241 Query: 243 TATNLTPPAPESLKMASVIVCSLPVLVLYPFLLKYFEKGTMIGSVKG 289 A N+ P ++++MASV++ SLPV+V+YPFL K+F KG ++GS+KG Sbjct: 242 NAENMMP--TQTMQMASVMIASLPVIVIYPFLQKHFTKGMLLGSIKG 286 >A9KLI5.1 A9KLI5_CLOPH Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 228 bits (581), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 108/277 (38%), Positives = 174/277 (62%), Gaps = 3/277 (1%) Query: 14 IIYLVLSLLALIVVYPFIHVTAVSFSGRGEALRSGFHFFPRDFEWRAYRELLEYPFIWSG 73 ++Y +L +A+I + PF+ V +S S A G H FP++ + Y+ +L Y IW+ Sbjct: 20 VVYAILLGVAIITIVPFLQVATISLSPAHVASSYGLHLFPKEIDLNGYKSILRYKTIWTS 79 Query: 74 YANTVFRMXXXXXXXXXXXXCAAYPLSRPDFPMKRVLIMLILFTMIFEGGIVPNYLLIKE 133 Y NT+ R AYPL++ P ++ + ++FTM F GG++P YLLI Sbjct: 80 YGNTIMRTVLGTGISMILYVTGAYPLAKKYLPHRKFWTLFVIFTMYFSGGMIPMYLLINN 139 Query: 134 -LHLLNSRWVYVLPTAANAFQVLVMLSFFRSIPDALIEAARIDGARDMRILFRIVLPLSI 192 L + N+ W VLP A +A+ ++++ +FF SIPD+L E+ARIDGA D+ ILF+I++PLS Sbjct: 140 WLKISNTIWALVLPGAMSAYNLIIVRNFFESIPDSLEESARIDGANDITILFKIIIPLSK 199 Query: 193 PSLVTIGLWSLVHHINAFMDNLLYVTDQSKYVLQQVVRQILIENKLDPFTTATNLTPPAP 252 P L T+ LW +V H N++ D +LY+ ++SKYVLQ +++ILI+ ++ + Sbjct: 200 PCLATVSLWCIVAHWNSWFDCMLYMKEESKYVLQYTLQRILIDGQVQDINLVDVVV--NT 257 Query: 253 ESLKMASVIVCSLPVLVLYPFLLKYFEKGTMIGSVKG 289 +S+KMA+++V +P++++YPF+ KYF G MIG+VKG Sbjct: 258 DSMKMAALMVSIIPIIIVYPFIQKYFTSGVMIGAVKG 294 >B3K469.1 B3K469_9BACI Binding-protein-dependent transport systems inner membrane component Length = 293 Score = 213 bits (543), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 112/275 (40%), Positives = 173/275 (62%), Gaps = 4/275 (1%) Query: 17 LVLSLLALIVVYPFIHVTAVSFSGRGEALRSGFHFFPRDFEWRAYRELLEYPFIWSGYAN 76 L+L+ LA+I VYP +++TAVS S E ++ FP+ F AY ++L + Y N Sbjct: 21 LILTCLAIITVYPVVYITAVSLSATSEVVQGNITLFPKGFNLDAYAQVLNDDRVPRAYLN 80 Query: 77 TVFRMXXXXXXXXXXXXCAAYPLSRPDFPMKRVLIMLILFTMIFEGGIVPNYLLIKELHL 136 T+F AAYPL+R +F ++ ++ I+ TM GI+PNYL++ EL L Sbjct: 81 TIFYTALGTAINLLLTAVAAYPLARKNFFGRKFFLLAIIMTMFLNPGIIPNYLIVSELGL 140 Query: 137 LNSRWVYVLPTAANAFQVLVMLSFFRSIPDALIEAARIDGARDMRILFRIVLPLSIPSLV 196 L+S W VLP A F++L++ SF+ ++ ++L EAA +DGA + RILF+IVLPLS P+L Sbjct: 141 LDSVWALVLPNAIWTFELLILKSFYENMSESLREAAVVDGASEYRILFQIVLPLSKPALA 200 Query: 197 TIGLWSLVHHINAFMDNLLYVTDQSKYVLQQVVRQILI--ENKLDPFTTATNLTPPAPES 254 +IGL+ + H N+F L+Y+ D + Y LQ V+R +LI EN +P + + + AP++ Sbjct: 201 SIGLFYFMGHWNSFFIPLIYLNDANMYPLQVVLRDMLIFSENGSNP--SLVDASALAPQA 258 Query: 255 LKMASVIVCSLPVLVLYPFLLKYFEKGTMIGSVKG 289 +K A++++ +PVL++YPF KYF KG M+GS KG Sbjct: 259 MKNATIVLSMIPVLLIYPFAQKYFAKGVMLGSEKG 293 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,608,660,435 Number of extensions: 59159658 Number of successful extensions: 282346 Number of sequences better than 1.0e-05: 4871 Number of HSP's gapped: 276215 Number of HSP's successfully gapped: 4871 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)