BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0013.p (339 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7E7.1 B3K7E7_9BACI Peptide chain release factor 2 580 e-164 B1DE93.1 B1DE93_9BACL Peptide chain release factor 2 549 e-154 P28367.2 RF2_BACSU Peptide chain release factor 2 505 e-141 Q65EC6.1 Q65EC6_BACLD PrfB 501 e-140 B7IPV0.1 B7IPV0_BACC2 Peptide chain release factor 2, programmed... 501 e-140 >B3K7E7.1 B3K7E7_9BACI Peptide chain release factor 2 Length = 331 Score = 580 bits (1495), Expect = e-164, Method: Compositional matrix adjust. Identities = 278/331 (83%), Positives = 296/331 (89%) Query: 9 MAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXXXXXXXXX 68 MAAP FWDD EKAQ +IAEMNAVKSSVDQY+KLQ +YDDA +M Sbjct: 1 MAAPDFWDDNEKAQALIAEMNAVKSSVDQYQKLQADYDDAVVMIELADEEGDDSLAGEIG 60 Query: 69 XTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYTRWAEKRG 128 TI+ + G+V+EFELQLLLNQPYDK+NAILELHPGAGGTESQDWGQML RMYTRWAEKRG Sbjct: 61 ETIKDLVGRVEEFELQLLLNQPYDKMNAILELHPGAGGTESQDWGQMLHRMYTRWAEKRG 120 Query: 129 FKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRRHTSFVSC 188 FKVE LDYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRRHTSFVSC Sbjct: 121 FKVETLDYLPGDEAGIKSVTLLIKGFNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC 180 Query: 189 DVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQ 248 DVVPEITDDVE++IRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQ Sbjct: 181 DVVPEITDDVEIDIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQ 240 Query: 249 IKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTS 308 IKNRE+AM MLRSKLYERK++EQQ++L EIRGEQSDIAWGSQIRSYVFHPYSMVKDHRT Sbjct: 241 IKNRERAMTMLRSKLYERKIEEQQKELAEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTQ 300 Query: 309 VETGNVGAVMDGDLDAFIDGYLRSQIKTEAE 339 VETGNVGAVMDGDLD FIDGYLRSQIK E E Sbjct: 301 VETGNVGAVMDGDLDGFIDGYLRSQIKQEVE 331 >B1DE93.1 B1DE93_9BACL Peptide chain release factor 2 Length = 358 Score = 549 bits (1414), Expect = e-154, Method: Compositional matrix adjust. Identities = 259/334 (77%), Positives = 287/334 (85%) Query: 2 IANFEEKMAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXX 61 IANFEEKM AP FWDD KAQG+I+E+NA+KS V+ YE+L + +D + Sbjct: 23 IANFEEKMTAPDFWDDNTKAQGIISELNAIKSVVEHYERLNADCEDLQTLIELADEESDD 82 Query: 62 XXXXXXXXTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYT 121 I S+ KV+ FELQLLLNQPYDKLNAILELHPGAGGTESQDW QML RMYT Sbjct: 83 SLEAEVIGGIASLNEKVNGFELQLLLNQPYDKLNAILELHPGAGGTESQDWAQMLYRMYT 142 Query: 122 RWAEKRGFKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRR 181 RWAEKRGFKVEVLDYL GDEAGIKSVT+ +KGYN YGYLKAEKGVHRL RISPFD+SGRR Sbjct: 143 RWAEKRGFKVEVLDYLDGDEAGIKSVTIAVKGYNAYGYLKAEKGVHRLVRISPFDSSGRR 202 Query: 182 HTSFVSCDVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVT 241 HTSFVSCDVVPEIT+D+EVEIRTEDLK+DTYRASGAGGQH+N T+SA+RITH+PTG+VV Sbjct: 203 HTSFVSCDVVPEITEDIEVEIRTEDLKVDTYRASGAGGQHVNKTESAIRITHIPTGIVVA 262 Query: 242 CQNERSQIKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSM 301 CQ ERSQI+NRE+AM MLRSKLYERK++EQQ+ L EIRGEQSDIAWGSQIRSYVFHPYSM Sbjct: 263 CQQERSQIQNRERAMNMLRSKLYERKIEEQQKHLAEIRGEQSDIAWGSQIRSYVFHPYSM 322 Query: 302 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQIK 335 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQI+ Sbjct: 323 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQIR 356 >P28367.2 RF2_BACSU Peptide chain release factor 2 Length = 366 Score = 505 bits (1301), Expect = e-141, Method: Compositional matrix adjust. Identities = 232/333 (69%), Positives = 276/333 (82%) Query: 2 IANFEEKMAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXX 61 IA +E+MA P FW+D +KAQ VI E N +K V+ Y+KL + +++ M Sbjct: 33 IAELDEQMADPEFWNDQQKAQTVINEANGLKDYVNSYKKLNESHEELQMTHDLLKEEPDT 92 Query: 62 XXXXXXXXTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYT 121 ++S+ + +EFELQLLL++PYDK NAILELHPGAGGTESQDWG MLLRMYT Sbjct: 93 DLQLELEKELKSLTKEFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYT 152 Query: 122 RWAEKRGFKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRR 181 RW E+RGFKVE LDYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRR Sbjct: 153 RWGERRGFKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRR 212 Query: 182 HTSFVSCDVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVT 241 HTSFVSC+V+PE D+++++IRTED+K+DTYRASGAGGQH+NTTDSAVRITHLPT VVVT Sbjct: 213 HTSFVSCEVMPEFNDEIDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNVVVT 272 Query: 242 CQNERSQIKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSM 301 CQ ERSQIKNRE+AMKML++KLY+R+++EQQ +LDEIRGEQ +I WGSQIRSYVFHPYSM Sbjct: 273 CQTERSQIKNRERAMKMLKAKLYQRRIEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSM 332 Query: 302 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQI 334 VKDHRT+ E GNV AVMDGD+D FID YLRS++ Sbjct: 333 VKDHRTNTEMGNVQAVMDGDIDTFIDAYLRSKL 365 >Q65EC6.1 Q65EC6_BACLD PrfB Length = 327 Score = 501 bits (1291), Expect = e-140, Method: Compositional matrix adjust. Identities = 231/326 (70%), Positives = 273/326 (83%) Query: 9 MAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXXXXXXXXX 68 MA P FW D +KAQ +I E NA+K V+ Y+ L + +++ M Sbjct: 1 MADPDFWGDQQKAQTIINEANALKDYVNTYKSLSESHEELQMTHDLLKEDPDQDLQNELV 60 Query: 69 XTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYTRWAEKRG 128 ++S+ + +EFELQLLL++PYDK NAILELHPGAGGTESQDWG MLLRMYTRWAE+RG Sbjct: 61 NELKSLTKQFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAERRG 120 Query: 129 FKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRRHTSFVSC 188 FKVE LDYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRRHTSFVSC Sbjct: 121 FKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC 180 Query: 189 DVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQ 248 DV+PE +++++EIRTED+K+DTYRASGAGGQH+NTTDSAVRITHLPTGV+VTCQ ERSQ Sbjct: 181 DVMPEFNEEIDIEIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTGVIVTCQTERSQ 240 Query: 249 IKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTS 308 IKNRE+AMKML+SKLY+R+++EQQ QLDEIRGEQ +I WGSQIRSYVFHPYS+VKDHRT+ Sbjct: 241 IKNRERAMKMLKSKLYQRRIEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN 300 Query: 309 VETGNVGAVMDGDLDAFIDGYLRSQI 334 E GNV AVMDGD+D+FID YLRS++ Sbjct: 301 TEMGNVQAVMDGDIDSFIDAYLRSKL 326 >B7IPV0.1 B7IPV0_BACC2 Peptide chain release factor 2, programmed frameshift Length = 380 Score = 501 bits (1291), Expect = e-140, Method: Compositional matrix adjust. Identities = 234/333 (70%), Positives = 272/333 (81%) Query: 2 IANFEEKMAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXX 61 IA EEKM + GFWDD + AQ VI E NA+K V + +L + +++ + Sbjct: 48 IAELEEKMMSAGFWDDQQGAQAVINEANALKDMVGSFRQLDETFENLEVTHELLKEEYDE 107 Query: 62 XXXXXXXXTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYT 121 ++ + +++E+ELQLLL+ PYDK NAILELHPGAGGTESQDWG MLLRMYT Sbjct: 108 DLHEELESEVKGLIQEMNEYELQLLLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYT 167 Query: 122 RWAEKRGFKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRR 181 RWAEKRGFKVE +DYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRR Sbjct: 168 RWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRR 227 Query: 182 HTSFVSCDVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVT 241 HTSFVSC+VVPE D+VE+E+RTEDLKIDTYRASGAGGQH+NTTDSAVRITH PT VVT Sbjct: 228 HTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVT 287 Query: 242 CQNERSQIKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSM 301 CQ+ERSQIKNRE AMKML++KLY++KL+EQQ QLDEIRGEQ +I WGSQIRSYVFHPYS+ Sbjct: 288 CQSERSQIKNREHAMKMLKAKLYQKKLEEQQAQLDEIRGEQKEIGWGSQIRSYVFHPYSL 347 Query: 302 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQI 334 VKDHRT+ E GNV AVMDG++D FID YLRS+I Sbjct: 348 VKDHRTNTEVGNVQAVMDGEIDPFIDAYLRSRI 380 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,909,779,719 Number of extensions: 74875220 Number of successful extensions: 271877 Number of sequences better than 1.0e-05: 3325 Number of HSP's gapped: 267353 Number of HSP's successfully gapped: 3327 Length of query: 339 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 203 Effective length of database: 1,779,438,163 Effective search space: 361225947089 Effective search space used: 361225947089 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)