BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0001.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7D5.1 B3K7D5_9BACI Putative esterase 384 e-105 B8Y562.1 B8Y562_9BACT Esterase 325 2e-87 A8M4Z2.1 A8M4Z2_SALAI Putative esterase 294 5e-78 B4D0X0.1 B4D0X0_9BACT Putative esterase 285 4e-75 C4RDM3.1 C4RDM3_9ACTO Esterase 273 8e-72 >B3K7D5.1 B3K7D5_9BACI Putative esterase Length = 257 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/260 (74%), Positives = 219/260 (84%), Gaps = 4/260 (1%) Query: 1 MALIECKFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLR 60 MAL++C FYS+VLGLSTSMTVILPQQT++QIGM+N K LHPTL+LLHGLSDDDSIWLR Sbjct: 1 MALMQCHFYSEVLGLSTSMTVILPQQTSSQIGMTNHAKSGLHPTLFLLHGLSDDDSIWLR 60 Query: 61 RTSIERYVAQMGIAVVMPQVHRSFYTDMAEGGRYWTFISEELPALARSFFPLSPKREDTF 120 RTSIERYVA+MGIAVVMPQVHRSFYTDM GG+YWTFIS+ELP +ARSFFPLS RED F Sbjct: 61 RTSIERYVAEMGIAVVMPQVHRSFYTDMEYGGKYWTFISQELPTIARSFFPLSHAREDNF 120 Query: 121 VAGLSMGGYGAVKLGLRKPDTFAAAASLSGALDMAHHFMNPEDPARKSPEYERVFGKQSI 180 VAGLSMGGYGA KL LR P++FAAAASLSGALDMA H E E+ V+G + I Sbjct: 121 VAGLSMGGYGAFKLALRCPESFAAAASLSGALDMAGHINRNE----ALFEHRLVYGDRDI 176 Query: 181 ENTPDDLLYLLKEVDRSKGPKPLLYQCCGTEDFLYENNQTFRKACAKTSLSLTYEEGPGE 240 T +DLL LL+EVDRS+GPKPLLYQCCGTEDFLY++NQ FRKAC TSL LTY+EGPG Sbjct: 177 TGTDNDLLRLLQEVDRSEGPKPLLYQCCGTEDFLYQDNQAFRKACEATSLDLTYQEGPGG 236 Query: 241 HEWGYWDTKIRDVLAWLPLE 260 H+WGYWDT I+DVL WLPL+ Sbjct: 237 HDWGYWDTHIQDVLKWLPLK 256 >B8Y562.1 B8Y562_9BACT Esterase Length = 270 Score = 325 bits (834), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 173/264 (65%), Positives = 200/264 (75%), Gaps = 7/264 (2%) Query: 1 MALIECKFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLR 60 MAL +C F+SDVLGLSTSMTVILPQ+TT QIGM+ ++ HPTL+LLHGLSDD +IWLR Sbjct: 1 MALFQCDFFSDVLGLSTSMTVILPQETTGQIGMAGGSERREHPTLFLLHGLSDDHTIWLR 60 Query: 61 RTSIERYVAQMGIAVVMPQVHRSFYTDMAEGGRYWTFISEELPALARSFFPLSPKREDTF 120 RTSIERYVA+MG+AVVMP VHRSFYTDMA G +YWTFISEELPALARSFFPL+ REDTF Sbjct: 61 RTSIERYVAEMGLAVVMPAVHRSFYTDMAHGLQYWTFISEELPALARSFFPLATAREDTF 120 Query: 121 VAGLSMGGYGAVKLGLRKPDTFAAAASLSGALDM----AHHFMNPEDPARKSPEYERVFG 176 VAGLSMGGYGA+KLG+R P+ FAAAASLSGALD+ A H +D E +FG Sbjct: 121 VAGLSMGGYGALKLGMRHPERFAAAASLSGALDITFDPAEHIAMEDD--VWVAEQRNIFG 178 Query: 177 K-QSIENTPDDLLYLLKEVDRSKGPKPLLYQCCGTEDFLYENNQTFRKACAKTSLSLTYE 235 ++ + DL L + + +S GP P LYQCCGTEDFLYE+N FR L YE Sbjct: 179 DLAALPGSDHDLFALAERMAQSDGPVPKLYQCCGTEDFLYEDNVRFRDHVRGLGLDFMYE 238 Query: 236 EGPGEHEWGYWDTKIRDVLAWLPL 259 E PGEHEWGYWD +I+ VLAWLPL Sbjct: 239 ESPGEHEWGYWDAQIQRVLAWLPL 262 >A8M4Z2.1 A8M4Z2_SALAI Putative esterase Length = 261 Score = 294 bits (753), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 160/261 (61%), Positives = 184/261 (70%), Gaps = 4/261 (1%) Query: 1 MALIECKFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLR 60 MAL FYS+VLGLSTSMTVILPQ T +QIGM+ V E P LYLLHGL+DDD++WLR Sbjct: 1 MALARVDFYSEVLGLSTSMTVILPQYTASQIGMAGVATVEDPPVLYLLHGLTDDDTVWLR 60 Query: 61 RTSIERYVAQMGIAVVMPQVHRSFYTDMAEGGRYWTFISEELPALARSFFPLSPKREDTF 120 RTSIERYVA +G+AVVMPQ RSFY D G RYWTF++EELPA+ FF LS +R DTF Sbjct: 61 RTSIERYVAPLGLAVVMPQAGRSFYCDEVHGNRYWTFLTEELPAICHGFFRLSRRRGDTF 120 Query: 121 VAGLSMGGYGAVKLGLRKPDTFAAAASLSGALDMAHHFMNPEDPARKSPEYERVFGKQSI 180 VAGLSMGGYGAVK LR PD FAAAASLSGALDMA +P P + + VFG+ I Sbjct: 121 VAGLSMGGYGAVKWALRHPDRFAAAASLSGALDMATRRHHPNRPV-DAGIWHTVFGEHVI 179 Query: 181 ENTPDDLLYLLKEVDRSKGPKPLLYQCCGTEDFLYENNQTFRKACAKTSLSLTYEEGPGE 240 T DD + LL DR+ P LY CGTEDFLYE+N F + +SLT + PGE Sbjct: 180 AGTDDDTVALLDRYDRA---GPALYLACGTEDFLYEDNVRFVDVARERGVSLTADFSPGE 236 Query: 241 HEWGYWDTKIRDVLAWLPLEK 261 H+W YWD KI+DVLAWLPL Sbjct: 237 HDWAYWDLKIQDVLAWLPLRH 257 >B4D0X0.1 B4D0X0_9BACT Putative esterase Length = 264 Score = 285 bits (728), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 142/263 (53%), Positives = 178/263 (67%), Gaps = 2/263 (0%) Query: 1 MALIECKFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLR 60 M+ ++ F+S+ L L+ S VILPQ TT QIGM++ + E +PTLYLLHGLSDD +IW R Sbjct: 1 MSFLDFHFFSESLALTCSAHVILPQATTRQIGMTSGKQRERYPTLYLLHGLSDDHTIWTR 60 Query: 61 RTSIERYVAQMGIAVVMPQVHRSFYTDMAEGGRYWTFISEELPALARSFFPLSPKREDTF 120 RTSIERY ++ IAVVMP V RSFY DMA G +YWTF+SEELPAL + +FPLS REDTF Sbjct: 61 RTSIERYASERNIAVVMPAVARSFYQDMASGPKYWTFLSEELPALCQHYFPLSDAREDTF 120 Query: 121 VAGLSMGGYGAVKLGLRKPDTFAAAASLSGALDMAHHFMNPEDPARK--SPEYERVFGKQ 178 AGLSMGGYGA++LGL +P+ FAA ASLSGALD+A + P + E+ + G Sbjct: 121 AAGLSMGGYGALRLGLARPEKFAAVASLSGALDLARRLREVDKPFARIDRKEWTGIIGPD 180 Query: 179 SIENTPDDLLYLLKEVDRSKGPKPLLYQCCGTEDFLYENNQTFRKACAKTSLSLTYEEGP 238 +L L +V S P+P +Y CGTED L ++TFR+ L LTY EGP Sbjct: 181 LKAPADSNLWLLASKVGASPEPRPAIYLSCGTEDELLSESRTFRQHLDTLGLPLTYSEGP 240 Query: 239 GEHEWGYWDTKIRDVLAWLPLEK 261 G HEWGYWD +I+ VL WLPL + Sbjct: 241 GAHEWGYWDAQIQRVLEWLPLRQ 263 >C4RDM3.1 C4RDM3_9ACTO Esterase Length = 263 Score = 273 bits (699), Expect = 8e-72, Method: Compositional matrix adjust. Identities = 149/263 (56%), Positives = 183/263 (69%), Gaps = 10/263 (3%) Query: 1 MALIECKFYSDVLGLSTSMTVILPQQTTTQIGMSNVTKGELHPTLYLLHGLSDDDSIWLR 60 MALI C F+S+ LG+ TSMTV+LP + + IGM+ P LYLLHGLSDDD+IW R Sbjct: 1 MALIRCDFHSEALGMGTSMTVLLPDRAGSGIGMAGGAPAGDPPVLYLLHGLSDDDTIWTR 60 Query: 61 RTSIERYVAQMGIAVVMPQVHRSFYTDMAEGGRYWTFISEELPALARSFFPLSPKREDTF 120 RTS+ERYVA +G+AVVMPQ RSFYTD G RYWTF+SEELP + RSFF LS +REDTF Sbjct: 61 RTSVERYVAPLGLAVVMPQAGRSFYTDEEHGNRYWTFLSEELPEVCRSFFRLSDRREDTF 120 Query: 121 VAGLSMGGYGAVKLGLRKPDTFAAAASLSGALDMAH---HFMNPEDPARKSPEYERVFGK 177 VAGLSMGGYGAVK LR+P FAAAASLSGALD+A H +P DPA + VFG Sbjct: 121 VAGLSMGGYGAVKWALREPGRFAAAASLSGALDVARRRDHPSHPMDPA----VWHTVFGD 176 Query: 178 QSIENTPDDLLYLLKEVDRSKGPKPLLYQCCGTEDFLYENNQTFRKACAKTSLSLTYEEG 237 + + + DD + LL+ R+ P LY CGTEDFL+++N F + + + LT + Sbjct: 177 RQVPGSDDDTVALLE---RAGDDLPALYVGCGTEDFLFDDNTRFVETARRRGVPLTVDFT 233 Query: 238 PGEHEWGYWDTKIRDVLAWLPLE 260 PG+H+W YWD KI+DVL WLPL Sbjct: 234 PGDHDWAYWDAKIQDVLTWLPLR 256 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,667,670,631 Number of extensions: 70000252 Number of successful extensions: 159556 Number of sequences better than 1.0e-05: 737 Number of HSP's gapped: 159414 Number of HSP's successfully gapped: 737 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)