BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0019.p (489 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BF74.1 Q2BF74_9BACI Sucrose phosphorylase 778 0.0 C2LT72.1 C2LT72_STRSL Sucrose phosphorylase EC=2.4.1.7 653 0.0 A8ZUP8.1 A8ZUP8_DESOH Sucrose phosphorylase EC=2.4.1.7 429 e-118 A8VXG7.1 A8VXG7_9BACI Sucrose phosphorylase EC=2.4.1.7 409 e-112 B9WVU8.1 B9WVU8_STRSU Sucrose phosphorylase EC=2.4.1.7 394 e-108 >Q2BF74.1 Q2BF74_9BACI Sucrose phosphorylase Length = 489 Score = 778 bits (2008), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/489 (74%), Positives = 417/489 (85%) Query: 1 MAIHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFE 60 MA+ NQVQLITYPDSLGG L+ LN V+ EHF+DIFKGG+HILPPFPSSGDRGFAPLTY E Sbjct: 1 MAVKNQVQLITYPDSLGGDLKMLNKVMTEHFADIFKGGVHILPPFPSSGDRGFAPLTYLE 60 Query: 61 IEPAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWA 120 I+P FG WEDIR IG D+L+D+MVNHIS++SEYFQDFL +GR+S YADLF+TLDK+W Sbjct: 61 IDPEFGGWEDIRKIGENFDILVDLMVNHISQKSEYFQDFLAKGRQSQYADLFITLDKLWE 120 Query: 121 GGQPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLL 180 GQPVQ DIDKMFLRRPLPYSTFTIEETGE+E VWTTFGKTDPSEQIDLDI S++V++LL Sbjct: 121 DGQPVQEDIDKMFLRRPLPYSTFTIEETGEQETVWTTFGKTDPSEQIDLDIKSEQVRQLL 180 Query: 181 ADIFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEV 240 D F NF+ NVKIVRLDAVGY++KKLGTSCFFVEPEIY FLDWI E+ +SL IELLPEV Sbjct: 181 TDFFTNFKEQNVKIVRLDAVGYVVKKLGTSCFFVEPEIYEFLDWIMELANSLGIELLPEV 240 Query: 241 HSHYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGIP 300 HSHYSIQ+KLAEHGFWIYDFILPYR+L+TL+NK+++ L YL RP KQFTMLDCHDGIP Sbjct: 241 HSHYSIQHKLAEHGFWIYDFILPYRVLETLINKSSKNLAIYLKNRPHKQFTMLDCHDGIP 300 Query: 301 VKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSYYSVLNSDDD 360 VKPDLDGLIDTKEA+ +VD CLERGSNLSLI+SDEHKAEDGFDVHQ+RCSYYSVLNSDDD Sbjct: 301 VKPDLDGLIDTKEAKDIVDVCLERGSNLSLILSDEHKAEDGFDVHQIRCSYYSVLNSDDD 360 Query: 361 AYLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKSA 420 AYLAARAIQFF PG+PQVYYVGLLAGEND INRHNF+++EIE++L K Sbjct: 361 AYLAARAIQFFTPGVPQVYYVGLLAGENDVENVARTGEGREINRHNFTLEEIEESLEKPV 420 Query: 421 VQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEWTKGDLRCTLYIDLSTNRTVIDYVET 480 VQRLL LIRFRNE AF+G+F+VL++ +E+RL W G C+L+IDL TNR+ I+YV Sbjct: 421 VQRLLKLIRFRNENEAFSGEFQVLDSPDSEIRLSWRNGSKHCSLFIDLETNRSTIEYVGE 480 Query: 481 EGMSVQFEV 489 G Q+ + Sbjct: 481 SGEMAQYNI 489 >C2LT72.1 C2LT72_STRSL Sucrose phosphorylase EC=2.4.1.7 Length = 487 Score = 653 bits (1685), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/478 (65%), Positives = 373/478 (78%), Gaps = 2/478 (0%) Query: 3 IHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFEIE 62 + N+VQLITYPDSLGG+L+ L LD +F ++F+GGIHILPP+PSSGDRGFAPLTYFEI+ Sbjct: 2 VKNKVQLITYPDSLGGNLKALKSNLDTYFPNLFEGGIHILPPYPSSGDRGFAPLTYFEID 61 Query: 63 PAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWAGG 122 P FG WED+R + +D+LLD+MVNHIS++S YFQDFL+ GR S YAD FLTL+KIW G Sbjct: 62 PQFGDWEDVRNLAEDYDILLDIMVNHISQKSPYFQDFLKNGRDSQYADYFLTLEKIWEDG 121 Query: 123 QPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLLAD 182 PVQ+DID+MFLRR +PYS F IE+TGE EKVWTTFGKT PSEQIDLD+HS++VK+L D Sbjct: 122 VPVQSDIDQMFLRRKVPYSEFIIEKTGEVEKVWTTFGKTTPSEQIDLDVHSEQVKQLFVD 181 Query: 183 IFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEVHS 242 IF++F +N VKI+RLDAVGY++KKLGTSCFFVEP+IY FLDWI V S DI LLPEVH+ Sbjct: 182 IFKHFHDNGVKIIRLDAVGYVLKKLGTSCFFVEPDIYDFLDWILGVAKSYDIALLPEVHA 241 Query: 243 HYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGIPVK 302 HYSIQYKLAEHG WIYDFILPYRI + L N+ ++ L +YL TRP QFTMLDCHDG+PVK Sbjct: 242 HYSIQYKLAEHGCWIYDFILPYRIFEALSNEESKFLVDYLRTRPHGQFTMLDCHDGLPVK 301 Query: 303 PDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSYYSVLNSDDDAY 362 PDLDGLID+K+AR+LVD L+RG+NLSLIVSDEHK EDGFDVHQ+R + YS+ + DDD Y Sbjct: 302 PDLDGLIDSKDARKLVDLALDRGANLSLIVSDEHKDEDGFDVHQIRSTIYSLFDEDDDTY 361 Query: 363 LAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKSAVQ 422 LAARA+Q F PGIPQ+YYVGLLAG+ND INRHNF EI A VQ Sbjct: 362 LAARALQVFTPGIPQIYYVGLLAGKNDIEAVEARGDGREINRHNFDEKEIAAAAETEVVQ 421 Query: 423 RLLALIRFRNEYGAFNGDFRVLEAASNEVRLEWTKGDLRC-TLYIDLSTNRTVIDYVE 479 RL+ LIRFRN++ AF GDF + E+ L W K D C +L IDL + Y E Sbjct: 422 RLVNLIRFRNQHPAFEGDFTAQAPSPTELVLSW-KNDAECASLTIDLKAKTGHVAYTE 478 >A8ZUP8.1 A8ZUP8_DESOH Sucrose phosphorylase EC=2.4.1.7 Length = 482 Score = 429 bits (1104), Expect = e-118, Method: Compositional matrix adjust. Identities = 213/484 (44%), Positives = 300/484 (61%), Gaps = 8/484 (1%) Query: 1 MAIHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFE 60 M + N++Q+I+YPDSLG +LQ L++VL +F+D GG+H+LP +PSS DRGFAP+TY++ Sbjct: 1 MTLRNRIQVISYPDSLGKNLQELHYVLRRYFADA-AGGVHLLPFYPSSADRGFAPVTYYQ 59 Query: 61 IEPAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWA 120 ++PAFG W DI I D+ +D MVNHISRQS+Y DF+ +G +S YAD+FL+ +K+ Sbjct: 60 VDPAFGTWRDIELIQEDFDITIDFMVNHISRQSKYVADFIEKGDKSEYADMFLSFNKLAP 119 Query: 121 GGQPVQADIDKMFLRRPLPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRLL 180 G+ + D+DK++ R+P P G E +W+TF EQID+D+ S + L Sbjct: 120 DGEIRKEDLDKVYTRKPSPPYQVIERPDGSRETIWSTFD----YEQIDVDVQSPVTRELF 175 Query: 181 ADIFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPEV 240 D + +R+DA Y +LGT+CFFVEPE++ L+ ++ ++LLPEV Sbjct: 176 RDFLIFLSRRKPEFIRMDAFAYTTIRLGTNCFFVEPEVWELLEMLEGFVSPFGVQLLPEV 235 Query: 241 HSHYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGIP 300 H HYS Q KLAE G+W+YDF LP +L TL + + L +L P +QFT LD HDG+ Sbjct: 236 HEHYSYQLKLAEKGYWVYDFALPMLVLHTLYHHTNKRLLHWLRICPRRQFTTLDTHDGLG 295 Query: 301 VKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSYYSVLNSDDD 360 V D+ L+ +E R VD E+GSN+ I S D++QV C+YYS L +DD Sbjct: 296 VV-DVADLMSQEEIERTVDGLYEKGSNIKRIYSTADY--QNLDIYQVNCTYYSALERNDD 352 Query: 361 AYLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKSA 420 +YLAAR +QF+APGIP VYYVGLLAGEND INRHN+++DEI A+ + Sbjct: 353 SYLAARTLQFYAPGIPHVYYVGLLAGENDVALVEATRNGRDINRHNYTLDEIAVAVEREV 412 Query: 421 VQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEWTKGDLRCTLYIDLSTNRTVIDYVET 480 VQRL+ L+RFR+ Y AF+G+ + E N V L W T +DL + RT I + + Sbjct: 413 VQRLIRLMRFRSSYPAFDGELVIEETPENRVALTWNMPPYTTTARVDLDSYRTEITFWDA 472 Query: 481 EGMS 484 + S Sbjct: 473 DTRS 476 >A8VXG7.1 A8VXG7_9BACI Sucrose phosphorylase EC=2.4.1.7 Length = 483 Score = 409 bits (1052), Expect = e-112, Method: Compositional matrix adjust. Identities = 204/479 (42%), Positives = 292/479 (60%), Gaps = 11/479 (2%) Query: 1 MAIHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFE 60 M + N++ LITY DSLG +L+ L+ VL+ HF D GG+H+LP +PSS DRGFAP+TY E Sbjct: 1 MTVKNEIMLITYADSLGKNLKELDEVLETHFKDAV-GGVHLLPFYPSSADRGFAPMTYQE 59 Query: 61 IEPAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWA 120 ++PAFG W D+ I K+D + D M+NHIS+ SEYFQDF+ + SPYADLF+ W Sbjct: 60 VDPAFGGWSDVERIAKKYDTMYDFMINHISKSSEYFQDFMEKKDDSPYADLFIQYKDFWP 119 Query: 121 GGQPVQADIDKMFLRRP-LPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRL 179 GG+P Q D+DK++ R+P PY T + G EK+W TF + EQIDL+++ ++ K Sbjct: 120 GGEPTQEDVDKIYKRKPRAPYIDVTFGD-GSTEKIWCTFDE----EQIDLNVYHERTKAF 174 Query: 180 LADIFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPE 239 + + I+RLDA Y KK GT CFF+EP+ + LD I+ + + + E+LPE Sbjct: 175 MRENLTFLAEKGASIIRLDAFAYATKKPGTDCFFLEPDTWEMLDEIQTILEPIGCEILPE 234 Query: 240 VHSHYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGI 299 +H HY+IQ KLAE G+++YDF LP +L L + + L +L P KQ+T LD HDGI Sbjct: 235 IHEHYTIQLKLAERGYYVYDFALPMLVLHALYSGRTDRLAHWLNIAPRKQYTTLDTHDGI 294 Query: 300 PVKPDLDGLIDTKEARRLVDTCLERGSNLSLIVSDEHKAEDGFDVHQVRCSYYSVLNSDD 359 V D+ L+ +E D +G+N+ + + + D++Q+ C+YYS L DD Sbjct: 295 GV-VDVADLMTQEEIEATRDDLFTKGANVKRVYNTMEY--NNLDIYQLNCTYYSALGDDD 351 Query: 360 DAYLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIEQALGKS 419 AYL ARAIQFFAPGIPQVYYVGLLAG+ND INRH ++ +EI + + + Sbjct: 352 QAYLLARAIQFFAPGIPQVYYVGLLAGKNDIELLEETKVGRNINRHYYTKEEIAEEMKRP 411 Query: 420 AVQRLLALIRFRNEYGAFNGDFRVLEAASNEV-RLEWTKGDLRCTLYIDLSTNRTVIDY 477 + + + L+ FRN+Y AF+ + E +V + T GD + L ++ T+ I Y Sbjct: 412 VMAKFMKLMTFRNDYKAFDDQIDITEREDGQVLEIVRTTGDNQAILTANVETHAFTITY 470 >B9WVU8.1 B9WVU8_STRSU Sucrose phosphorylase EC=2.4.1.7 Length = 482 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 207/488 (42%), Positives = 289/488 (59%), Gaps = 22/488 (4%) Query: 1 MAIHNQVQLITYPDSLGGSLQTLNHVLDEHFSDIFKGGIHILPPFPSSGDRGFAPLTYFE 60 M I NQ LITY DSLG +L+ L VL+ D+ GGIHILP FPSSGDRGFAP+ Y + Sbjct: 1 MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVV-GGIHILPFFPSSGDRGFAPMDYTK 59 Query: 61 IEPAFGAWEDIRAIGAKHDVLLDVMVNHISRQSEYFQDFLRQGRRSPYADLFLTLDKIWA 120 ++PAFG W DI A+ + ++ D M+NHIS +S YF DFL + S YADLF+ W Sbjct: 60 VDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKKDESDYADLFIRYKNFWP 119 Query: 121 GGQPVQADIDKMFLRRP-LPYSTFTIEETGEEEKVWTTFGKTDPSEQIDLDIHSDKVKRL 179 G+P Q D+D ++ R+P PY T + G EEKVW TF + +QIDLD+ ++ +R Sbjct: 120 NGEPTQEDVDLIYKRKPRAPYRIATFAD-GSEEKVWCTFDE----QQIDLDVTTETTRRF 174 Query: 180 LADIFENFRNNNVKIVRLDAVGYIIKKLGTSCFFVEPEIYGFLDWIKEVTDSLDIELLPE 239 + + E + I+RLDA Y KKLGT+CFFVEP+I+ L + +++ D+E+LPE Sbjct: 175 IQENLEQLAEHGASIIRLDAFAYANKKLGTNCFFVEPDIWEMLHFPRQLLAPKDVEILPE 234 Query: 240 VHSHYSIQYKLAEHGFWIYDFILPYRILDTLVNKNAEALKEYLTTRPAKQFTMLDCHDGI 299 +H HY+IQ K+AE +++YDF LP +L L + + L ++ P KQFT LD HDGI Sbjct: 235 IHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHWMEICPRKQFTTLDTHDGI 294 Query: 300 PVKPDLDGLIDTKEARRLVDTCLER------GSNLSLIVSDEHKAEDGFDVHQVRCSYYS 353 G++D K+ +T R G+N+ I S E A + D++Q+ C+YYS Sbjct: 295 -------GVVDVKDLLTDEETEETREALYAQGANVKKIYSTE--AYNNLDIYQINCTYYS 345 Query: 354 VLNSDDDAYLAARAIQFFAPGIPQVYYVGLLAGENDXXXXXXXXXXXXINRHNFSIDEIE 413 L ++D AYL AR +Q FAPGIPQ+YYVGLLAGEND INRH + ++EIE Sbjct: 346 ALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGRNINRHYYDLEEIE 405 Query: 414 QALGKSAVQRLLALIRFRNEYGAFNGDFRVLEAASNEVRLEWTKGDLRCTLYIDLSTNRT 473 Q + + VQ L L++FRN AF+G+F V + + W D + + + Sbjct: 406 QEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNNQDAGVSARLTANLKEK 465 Query: 474 VIDYVETE 481 + VE E Sbjct: 466 SFEIVEIE 473 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,984,337,939 Number of extensions: 126931697 Number of successful extensions: 272311 Number of sequences better than 1.0e-05: 352 Number of HSP's gapped: 271986 Number of HSP's successfully gapped: 354 Length of query: 489 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 349 Effective length of database: 1,741,754,103 Effective search space: 607872181947 Effective search space used: 607872181947 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)