BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0014.p (291 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIA8.1 B3KIA8_9BACI Binding-protein-dependent transport system... 218 4e-55 B3KFT0.1 B3KFT0_9BACI Binding-protein-dependent transport system... 198 4e-49 B4ANQ2.1 B4ANQ2_BACPU Protein LplC 196 3e-48 B3KF75.1 B3KF75_9BACI Binding-protein-dependent transport system... 196 3e-48 A8FGF7.1 A8FGF7_BACP2 ABC superfamily ATP binding cassette trans... 195 3e-48 >B3KIA8.1 B3KIA8_9BACI Binding-protein-dependent transport systems inner membrane component Length = 287 Score = 218 bits (556), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 118/291 (40%), Positives = 186/291 (63%), Gaps = 6/291 (2%) Query: 2 MRTKISLFDICNNIFLLMIALVCTYPFLYIFFLSISDGRSIASGDVAFFPKNVNFEAYQY 61 M+ K + FD N + L + C YPF+Y+ +S+SD SIASG+V +PK N + YQY Sbjct: 1 MKPKWNWFDALNALILTGVVGACLYPFVYMLAVSLSDSASIASGEVWLWPKGFNLDMYQY 60 Query: 62 ILTNSKLGIFRGLLNSGIYXXXXXXXXXXXXYMTAYVLSR-KQIKGRFLIMSLFVFSWVF 120 + + + + +G N+ IY + AY LS+ K + G+ ++M L VF+ F Sbjct: 61 VFEDGR--VLKGYKNTLIYVVLGTTISLLVTALGAYSLSKAKLVMGKPILM-LIVFTMFF 117 Query: 121 EAGIIPAYIVLNKLGFVDNWLVMIIPNAINVQFLIITKTFLDGLPVELEEAAKIDGANDF 180 G+IP ++V+ +LGFV+ M++P A+ L+I +TF G+P ELEE+ KIDG ++ Sbjct: 118 NGGMIPTFLVVKELGFVNTIWGMVLPGAVATWNLLIMRTFFMGMPQELEESGKIDGLSEI 177 Query: 181 QIMSRVFLAISKPILATIGVFYAVFIWNQYLMPLIYLQNPNLQTIQVMLKKLVISSGDSN 240 I R+ L +SKP+LATIG++YAV +WN ++ PL+YL++ ++Q +QV+L+ +V+S + Sbjct: 178 GIFFRIVLPLSKPVLATIGLYYAVGMWNNFMGPLLYLRDADMQPLQVILRNIVLSGQLTG 237 Query: 241 TAFRTIIQDGIMLNPGNLKAAAIFVAMVPIVLVYPFVQKYFKKGILLGSVK 291 T ++ D +++ G LK A I V+ +PI+LVYPF+QKYF KG L+GSVK Sbjct: 238 TD-GPVVGDIVVVEDG-LKFATIMVSTLPILLVYPFIQKYFVKGALIGSVK 286 >B3KFT0.1 B3KFT0_9BACI Binding-protein-dependent transport systems inner membrane component Length = 305 Score = 198 bits (504), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 105/289 (36%), Positives = 178/289 (61%), Gaps = 16/289 (5%) Query: 9 FDICNNIFLLMIALVCTYPFLYIFFLSISDGRSIASGDVAFFPKNVNFEAYQYILTNS-- 66 F + N F +++ L PFL + S+S ++ SG V P V FE Y+Y+L +S Sbjct: 26 FSVFNYTFFIVMGLTTLLPFLNLIAKSLSSEGAVISGKVGLLPVGVQFETYKYVLQDSMF 85 Query: 67 ----KLGIFRGLLNSGIYXXXXXXXXXXXXYMTAYVLSRKQIKGRFLIMSLFVFSWVFEA 122 K+ IF ++ + +TAY LS+ +++GR ++ +FVF+ +F Sbjct: 86 LNSLKVSIFLTIIGTACSLFLTT--------LTAYPLSKYRLRGRKGLLLIFVFTMLFSG 137 Query: 123 GIIPAYIVLNKLGFVDNWLVMIIPNAINVQFLIITKTFLDGLPVELEEAAKIDGANDFQI 182 G+IP Y+++ L V+++ V+ +P +NV ++I K + +GLP ELEE+AK+DGA + +I Sbjct: 138 GLIPTYLLMQNLNLVNSFPVLFLPAMVNVYNMLIIKNYFEGLPDELEESAKLDGAGNIRI 197 Query: 183 MSRVFLAISKPILATIGVFYAVFIWNQYLMPLIYLQNPNLQTIQVMLKKLVISSGDSNTA 242 + V + +S P++ATIG+F+AV WN Y +IY+ +P ++ +Q+ LK+L++SS S Sbjct: 198 LVSVIVPLSLPVMATIGLFFAVAFWNDYFAAMIYITDPAIKPMQLYLKELLVSS--SGDF 255 Query: 243 FRTIIQDGIMLNPGNLKAAAIFVAMVPIVLVYPFVQKYFKKGILLGSVK 291 + + I P +++A++I +A +PI+LVYPF+QKYF KG+L+GSVK Sbjct: 256 LKDNVDAAINATPQSIQASSILLATIPILLVYPFLQKYFVKGVLVGSVK 304 >B4ANQ2.1 B4ANQ2_BACPU Protein LplC Length = 292 Score = 196 bits (497), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 115/292 (39%), Positives = 168/292 (57%), Gaps = 13/292 (4%) Query: 5 KISLFDICNNIFLLMIALVCTYPFLYIFFLSISDGRSIASGDVAFFPKNVNFEAYQYILT 64 K LF++ N FLLM AL+C PF+++ S + + + FP + EAY+YI + Sbjct: 8 KDRLFNLFNYSFLLMFALICVLPFIHVIAASFATVEEVITKKFILFPTTFSLEAYRYIFS 67 Query: 65 NSKLGIFRGLLNSGIYXXXXXXXXXXXXYMTAYVLSRKQIKGRFLIMSLFVFSWVFEAGI 124 ++R L+ S + + AY LSR+++KGR +M L VF+ +F G+ Sbjct: 68 TDI--VYRSLIVSILVTLVGTAVSMFLSSLMAYGLSRQELKGRRTLMFLVVFTMLFSGGM 125 Query: 125 IPAYIVLNKLGFVDNWLVMIIPNAINVQFLIITKTFLDGLPVELEEAAKIDGANDFQIMS 184 IP +IV+ LG +D + +I+P AIN LII K F +P LEE+AK+DG ND I Sbjct: 126 IPTFIVVKTLGLLDTYWSLILPVAINAFNLIILKNFFQNIPASLEESAKMDGCNDLGIFF 185 Query: 185 RVFLAISKPILATIGVFYAVFIWNQYLMPLIYLQNPNLQTIQVMLKKLVISS----GD-S 239 ++ L +S P +ATI +FYAV WN Y+ ++YL + IQV+L+++VI S GD S Sbjct: 186 KIVLPLSLPAIATISLFYAVTYWNTYMTAILYLNDSVKWPIQVLLRQIVIVSSGMQGDMS 245 Query: 240 NTAFRTIIQDGIMLNPGNLKAAAIFVAMVPIVLVYPFVQKYFKKGILLGSVK 291 + T D +K A I VA +P++LVYPF+QK+F KG LLGSVK Sbjct: 246 DMGSSTPPPD------QTIKMAVIVVATIPVLLVYPFIQKHFNKGALLGSVK 291 >B3KF75.1 B3KF75_9BACI Binding-protein-dependent transport systems inner membrane component Length = 291 Score = 196 bits (497), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 103/293 (35%), Positives = 185/293 (63%), Gaps = 5/293 (1%) Query: 1 MMRTKISLFDICNNIFLLMIA--LVCTYPFLYIFFLSISDGRSIASGDVAFFPKNVNFEA 58 M R + F + +I++L+IA L+ PF+ +F SIS+ +I SG V FP E Sbjct: 1 MYRKTVKDFMLDGSIYVLLIAMGLITLLPFVNVFSKSISEEWAILSGQVGIFPVGFQLET 60 Query: 59 YQYILTNSKLGIFRGLLNSGIYXXXXXXXXXXXXYMTAYVLSRKQIKGRFLIMSLFVFSW 118 ++++TNS+ L S + +TAY LS++++ G +I+ LF+F+ Sbjct: 61 MKFVVTNSQF--LNSLWISVLITVIGTLASLLITALTAYPLSKRELPGMGIIIILFIFTM 118 Query: 119 VFEAGIIPAYIVLNKLGFVDNWLVMIIPNAINVQFLIITKTFLDGLPVELEEAAKIDGAN 178 +F GIIP Y+++ +LG +++ +I+P I+V +++ K++ + LP LEE+AKI+GA+ Sbjct: 119 MFNGGIIPNYLLIRELGLINHLGSLILPALISVFNMLVIKSYFESLPASLEESAKIEGAS 178 Query: 179 DFQIMSRVFLAISKPILATIGVFYAVFIWNQYLMPLIYLQNPNLQTIQVMLKKLVISSGD 238 + ++ R+ L +S P+LATIG+FYAV+ WN Y P++Y+ + +L+ +Q+ L+ +V+++ D Sbjct: 179 NLTVLFRIILPLSGPVLATIGLFYAVYYWNDYFNPMLYINSSSLKPLQLYLQDIVMNA-D 237 Query: 239 SNTAFRTIIQDGIMLNPGNLKAAAIFVAMVPIVLVYPFVQKYFKKGILLGSVK 291 +++A D + + +++A + + +PI+LVYPF+QKYF KG+L+GSVK Sbjct: 238 TSSALDKTADDLMNVPAEGVRSATVIASTIPILLVYPFLQKYFIKGVLIGSVK 290 >A8FGF7.1 A8FGF7_BACP2 ABC superfamily ATP binding cassette transporter, membrane protein Length = 292 Score = 195 bits (496), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 115/292 (39%), Positives = 168/292 (57%), Gaps = 13/292 (4%) Query: 5 KISLFDICNNIFLLMIALVCTYPFLYIFFLSISDGRSIASGDVAFFPKNVNFEAYQYILT 64 K LF++ N FLLM AL+C PF+++ S + + + FP + EAY+YI + Sbjct: 8 KGRLFNLFNYSFLLMFALICVLPFIHVIAASFATVEEVITKKFILFPTTFSLEAYRYIFS 67 Query: 65 NSKLGIFRGLLNSGIYXXXXXXXXXXXXYMTAYVLSRKQIKGRFLIMSLFVFSWVFEAGI 124 ++R L+ S + + AY LSR+++KGR +M L VF+ +F G+ Sbjct: 68 TDI--VYRSLIVSILVTLVGTAVSMFLSSLMAYGLSRQELKGRRTLMFLVVFTMLFSGGM 125 Query: 125 IPAYIVLNKLGFVDNWLVMIIPNAINVQFLIITKTFLDGLPVELEEAAKIDGANDFQIMS 184 IP +IV+ LG +D + +I+P AIN LII K F +P LEE+AK+DG ND I Sbjct: 126 IPTFIVVKTLGLLDTYWSLILPVAINAFNLIILKNFFQNIPASLEESAKMDGCNDLGIFF 185 Query: 185 RVFLAISKPILATIGVFYAVFIWNQYLMPLIYLQNPNLQTIQVMLKKLVISS----GD-S 239 ++ L +S P +ATI +FYAV WN Y+ ++YL + IQV+L+++VI S GD S Sbjct: 186 KIVLPLSLPAIATISLFYAVTYWNTYMTAILYLNDSVKWPIQVLLRQIVIVSSGMQGDMS 245 Query: 240 NTAFRTIIQDGIMLNPGNLKAAAIFVAMVPIVLVYPFVQKYFKKGILLGSVK 291 + T D +K A I VA +P++LVYPF+QK+F KG LLGSVK Sbjct: 246 DMGSSTPPPD------QTIKMAVIVVATIPVLLVYPFIQKHFNKGALLGSVK 291 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.145 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,497,683,886 Number of extensions: 55195003 Number of successful extensions: 197405 Number of sequences better than 1.0e-05: 5378 Number of HSP's gapped: 190861 Number of HSP's successfully gapped: 5378 Length of query: 291 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 157 Effective length of database: 1,798,280,193 Effective search space: 282329990301 Effective search space used: 282329990301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)