BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0010.p (335 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4ITC1.1 A4ITC1_GEOTN Pyridoxal phosphate biosynthesis 471 e-131 Q5WLA7.1 Q5WLA7_BACSK 4-hydroxythreonine-4-phosphate dehydrogena... 444 e-123 Q65EJ5.1 Q65EJ5_BACLD Putative uncharacterized protein 444 e-123 B3K3L0.1 B3K3L0_9BACI 4-hydroxythreonine-4-phosphate dehydrogena... 440 e-122 B1DGN3.1 B1DGN3_9BACL 4-hydroxythreonine-4-phosphate dehydrogena... 424 e-117 >A4ITC1.1 A4ITC1_GEOTN Pyridoxal phosphate biosynthesis Length = 331 Score = 471 bits (1211), Expect = e-131, Method: Compositional matrix adjust. Identities = 228/332 (68%), Positives = 273/332 (82%), Gaps = 1/332 (0%) Query: 2 MKPIIAITMGDAAGIGPEVIVKALSHPDVYEMCRPVVIGDAGTLKRAAAVTGRSDTGIHP 61 MKP I ITMGDAAG+GPE+IVKAL + +YE C P+VIGDA L+RA +T T + P Sbjct: 1 MKPTIGITMGDAAGVGPEIIVKALQNSSIYEQCHPLVIGDAKILERAVRITNAGLT-VRP 59 Query: 62 VAGVAEALFQQGIIDCINLNLLPDTVPFGQLSAAAGNAAFHYIAKAVELAKAGEVQAICT 121 ++ +AEA ++ G+IDC++L+LLP +PFGQ+SAAAG+AAF ++ +A+ELA+ GE+ AICT Sbjct: 60 ISDIAEADYRYGMIDCLHLDLLPVDLPFGQVSAAAGDAAFQFLKRAIELAQNGEIDAICT 119 Query: 122 APLNKEALHKGGHLYPGHTEILAQLTETEDFSMMLSTDRLKVIHLTTHVGLIKAVEMITP 181 APLNKEA+HKGGHLYPGHTEILA LT TED++MMLS+ +LKVIHLTTHVGLI+A+EMI P Sbjct: 120 APLNKEAMHKGGHLYPGHTEILADLTGTEDYAMMLSSPQLKVIHLTTHVGLIQAIEMINP 179 Query: 182 ERTYNVLKLADQVLRTAGNEHPRIALCGINPHAXXXXXXXXXXXXXKLLPAVEKARAEGM 241 ERTY V+KLA L AG EHP IA+CGINPHA KL+PA+ KA+AEG+ Sbjct: 180 ERTYKVIKLAHDTLTRAGKEHPSIAVCGINPHAGENGLFGNGEEEEKLIPAIHKAQAEGI 239 Query: 242 EVYGPAPADTLFYRAMRGDFDIVVACYHDQGHTPIKVLGIEAGVNITVGLKGGIIRTSVD 301 V GP PADTLFYRA+RGDFDIVVACYHDQGH PIKVLG+EAGVNITVGLKGGIIRTSVD Sbjct: 240 NVTGPYPADTLFYRAVRGDFDIVVACYHDQGHAPIKVLGLEAGVNITVGLKGGIIRTSVD 299 Query: 302 HGTAFDIAGKGIADERSMLAALRSAVELAPRQ 333 HGTAFDIAGK +ADERSML A+R AVELAP++ Sbjct: 300 HGTAFDIAGKNLADERSMLEAIRVAVELAPKR 331 >Q5WLA7.1 Q5WLA7_BACSK 4-hydroxythreonine-4-phosphate dehydrogenase EC=1.1.1.262 Length = 330 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/331 (65%), Positives = 261/331 (78%), Gaps = 1/331 (0%) Query: 2 MKPIIAITMGDAAGIGPEVIVKALSHPDVYEMCRPVVIGDAGTLKRAAAVTGRSDTGIHP 61 M+PII ITMGDAAG+GPE+I KAL+ +++ RPVVIGDA ++RA +T ++ + Sbjct: 1 MRPIIGITMGDAAGVGPEIIAKALADESTFKIGRPVVIGDATIMERAVDIT-ETELNVVS 59 Query: 62 VAGVAEALFQQGIIDCINLNLLPDTVPFGQLSAAAGNAAFHYIAKAVELAKAGEVQAICT 121 V+ V E +F+ G I+ I+L LL + V FGQ+S AAGNAAF Y+ +AVELAKA ++ AICT Sbjct: 60 VSTVDECVFEPGTIEVIDLRLLTEGVSFGQVSEAAGNAAFRYVERAVELAKAKKINAICT 119 Query: 122 APLNKEALHKGGHLYPGHTEILAQLTETEDFSMMLSTDRLKVIHLTTHVGLIKAVEMITP 181 APLNKEA+ K GHLYPGHTEILA+LT T DFSMMLS LKVIHLTTHVGL KA++ I P Sbjct: 120 APLNKEAMQKAGHLYPGHTEILAELTGTTDFSMMLSAPNLKVIHLTTHVGLRKAIDTINP 179 Query: 182 ERTYNVLKLADQVLRTAGNEHPRIALCGINPHAXXXXXXXXXXXXXKLLPAVEKARAEGM 241 ERTY V+ LA + L AGN+ P+IA+CGINPHA KL+PA+EKAR EG+ Sbjct: 180 ERTYKVISLAHETLAAAGNKQPKIAVCGINPHAGENGLFGEGEEEEKLVPAIEKARGEGI 239 Query: 242 EVYGPAPADTLFYRAMRGDFDIVVACYHDQGHTPIKVLGIEAGVNITVGLKGGIIRTSVD 301 +V GP PADTLF+RA RGDFDIVVACYHDQGH PIKV+G+EAGVNITVGLKGGI+RTSVD Sbjct: 240 DVEGPYPADTLFFRAARGDFDIVVACYHDQGHVPIKVMGLEAGVNITVGLKGGIVRTSVD 299 Query: 302 HGTAFDIAGKGIADERSMLAALRSAVELAPR 332 HGTAFDIAGK IADERS+LAA R AVELAP+ Sbjct: 300 HGTAFDIAGKNIADERSLLAAYRDAVELAPK 330 >Q65EJ5.1 Q65EJ5_BACLD Putative uncharacterized protein Length = 332 Score = 444 bits (1142), Expect = e-123, Method: Compositional matrix adjust. Identities = 219/333 (65%), Positives = 262/333 (78%), Gaps = 1/333 (0%) Query: 1 MMKPIIAITMGDAAGIGPEVIVKALSHPDVYEMCRPVVIGDAGTLKRAAAVTGRSDTGIH 60 M KP+I ITMGDAAG+GPE+IVKAL+ +VY+MCRP+VIGD LK+AA G S+ I+ Sbjct: 1 MKKPVIGITMGDAAGVGPEIIVKALTSREVYDMCRPLVIGDMKMLKKAAFQIG-SEVVIN 59 Query: 61 PVAGVAEALFQQGIIDCINLNLLPDTVPFGQLSAAAGNAAFHYIAKAVELAKAGEVQAIC 120 V E F G IDC+++ LLP+ +P G++SA AGNAAFH++ KAVELAK G +QAIC Sbjct: 60 KVGHPDEGGFVHGKIDCLDIELLPENLPVGKVSAEAGNAAFHFVKKAVELAKKGCIQAIC 119 Query: 121 TAPLNKEALHKGGHLYPGHTEILAQLTETEDFSMMLSTDRLKVIHLTTHVGLIKAVEMIT 180 TAPLNKEALHKGGH YPGHTEILA LT T D+SMML++ +LKVIHLTTH GLI+A++ IT Sbjct: 120 TAPLNKEALHKGGHRYPGHTEILAHLTNTTDYSMMLASPKLKVIHLTTHQGLIEAIQSIT 179 Query: 181 PERTYNVLKLADQVLRTAGNEHPRIALCGINPHAXXXXXXXXXXXXXKLLPAVEKARAEG 240 PERTY V++LA L AG E P+IA+CGINPHA +LLP +E+A AEG Sbjct: 180 PERTYKVIRLAHDTLTRAGFEQPKIAVCGINPHAGENGLFGSGEEERQLLPGIEQAAAEG 239 Query: 241 MEVYGPAPADTLFYRAMRGDFDIVVACYHDQGHTPIKVLGIEAGVNITVGLKGGIIRTSV 300 ++ GP PADTLFY+A+RGD D+VVACYHDQGH P+KVLG+E GVNITVGL GGIIRTSV Sbjct: 240 IDAGGPYPADTLFYKAVRGDVDLVVACYHDQGHIPVKVLGLEEGVNITVGLNGGIIRTSV 299 Query: 301 DHGTAFDIAGKGIADERSMLAALRSAVELAPRQ 333 DHGTAFDIAG+ ADERSMLAALRSA ELAP+ Sbjct: 300 DHGTAFDIAGQNKADERSMLAALRSAAELAPKS 332 >B3K3L0.1 B3K3L0_9BACI 4-hydroxythreonine-4-phosphate dehydrogenase EC=1.1.1.262 Length = 333 Score = 440 bits (1132), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/331 (63%), Positives = 267/331 (80%), Gaps = 1/331 (0%) Query: 2 MKPIIAITMGDAAGIGPEVIVKALSHPDVYEMCRPVVIGDAGTLKRAAAVTGRSDTGIHP 61 MKP + ITMGDAAGIGPE+I+KALSH ++Y+ C P+VIGDA L+R + G S ++ Sbjct: 1 MKPTVGITMGDAAGIGPEIIMKALSHQEMYDNCNPLVIGDAKILERVLPIIGSS-LKVNA 59 Query: 62 VAGVAEALFQQGIIDCINLNLLPDTVPFGQLSAAAGNAAFHYIAKAVELAKAGEVQAICT 121 + AEA ++ G +D I+L+L+P + FG++SA AG+AAF ++A+A++LAK ++ +ICT Sbjct: 60 IQEPAEATYEFGTVDVIDLDLVPADLAFGEVSAVAGDAAFQFLARAIDLAKQRKIHSICT 119 Query: 122 APLNKEALHKGGHLYPGHTEILAQLTETEDFSMMLSTDRLKVIHLTTHVGLIKAVEMITP 181 APLNKEALH+GGH+YPGHTEILA LT+T+DFSMML+T L+VIHLTTH+GLI+A++ I P Sbjct: 120 APLNKEALHQGGHMYPGHTEILADLTDTKDFSMMLTTPNLRVIHLTTHMGLIEAIQSINP 179 Query: 182 ERTYNVLKLADQVLRTAGNEHPRIALCGINPHAXXXXXXXXXXXXXKLLPAVEKARAEGM 241 ERTY V+KLA L+ AG ++PR+A+CGINPHA KLLP +++A+ EG+ Sbjct: 180 ERTYTVVKLAHDTLKKAGFDNPRVAVCGINPHAGENGLFGNGEEEEKLLPGIQRAQQEGI 239 Query: 242 EVYGPAPADTLFYRAMRGDFDIVVACYHDQGHTPIKVLGIEAGVNITVGLKGGIIRTSVD 301 V GP PADTLF+RA RGDFDIVVACYHDQGH PIKV+GIE GVNITVGLKGGIIRTSVD Sbjct: 240 NVVGPLPADTLFFRAGRGDFDIVVACYHDQGHAPIKVMGIEEGVNITVGLKGGIIRTSVD 299 Query: 302 HGTAFDIAGKGIADERSMLAALRSAVELAPR 332 HGTAFDIAGK IAD++SMLAA+RSA+ELAP+ Sbjct: 300 HGTAFDIAGKNIADDKSMLAAIRSAIELAPK 330 >B1DGN3.1 B1DGN3_9BACL 4-hydroxythreonine-4-phosphate dehydrogenase EC=1.1.1.262 Length = 329 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 211/332 (63%), Positives = 253/332 (76%), Gaps = 3/332 (0%) Query: 2 MKPIIAITMGDAAGIGPEVIVKALSHPDVYEMCRPVVIGDAGTLKRAAAVTGRSDTGIHP 61 MK II ITMGD AG+GPE+I+KAL VYE P VIGDA L+RAA V S+ + P Sbjct: 1 MKAIIGITMGDGAGVGPEIIMKALGDNKVYEDSNPFVIGDAKILERAARVV-NSELKVRP 59 Query: 62 VAGVAEALFQQGIIDCINLNLLPDTVPFGQLSAAAGNAAFHYIAKAVELAKAGEVQAICT 121 +A V EA ++ G +DC++LNLLP +PFGQ+S AG+AAF Y+ A+ELA G + ICT Sbjct: 60 IASVTEAQYEYGTVDCLDLNLLPADLPFGQVSPEAGHAAFMYLKTAIELANEGLIDGICT 119 Query: 122 APLNKEALHKGGHLYPGHTEILAQLTETEDFSMMLSTDRLKVIHLTTHVGLIKAVEMITP 181 APLNKEALHKGGH+YPGHTEILA LTET+DF+MMLS +LKVIH+TTHVG+I AV MI P Sbjct: 120 APLNKEALHKGGHIYPGHTEILADLTETQDFAMMLSAPKLKVIHVTTHVGIIDAVRMIEP 179 Query: 182 ERTYNVLKLADQVLRTAGNEHPRIALCGINPHAXXXXXXXXXXXXXKLLPAVEKARAEGM 241 ER Y V+++A Q LR AG PRIA+CGINPHA K++P V++A+ EG+ Sbjct: 180 ERVYKVIQMAHQTLRKAGYAEPRIAVCGINPHAGENGLFGYGEEEQKVIPGVQRAQREGI 239 Query: 242 EVYGPAPADTLFYRAMRGDFDIVVACYHDQGHTPIKVLGIEAGVNITVGLKGGIIRTSVD 301 V GP PADTLF+R RGDFDIVVA YHDQGH P+KVLG+EAGVNITVGL IIRTSVD Sbjct: 240 NVQGPLPADTLFFRTTRGDFDIVVAMYHDQGHGPVKVLGLEAGVNITVGLP--IIRTSVD 297 Query: 302 HGTAFDIAGKGIADERSMLAALRSAVELAPRQ 333 HGTAFDIAGKGIAD++S+ ALR AVELAP++ Sbjct: 298 HGTAFDIAGKGIADDQSIKEALRQAVELAPKR 329 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,855,975,489 Number of extensions: 72840317 Number of successful extensions: 169296 Number of sequences better than 1.0e-05: 1073 Number of HSP's gapped: 165232 Number of HSP's successfully gapped: 1075 Length of query: 335 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 199 Effective length of database: 1,779,438,163 Effective search space: 354108194437 Effective search space used: 354108194437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)