BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0009.p (453 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4ITC0.1 A4ITC0_GEOTN Putative uncharacterized protein 296 3e-78 B3K3L1.1 B3K3L1_9BACI Type III effector Hrp-dependent outer protein 288 1e-75 Q65EJ4.1 Q65EJ4_BACLD Putative uncharacterized protein 258 6e-67 Q3ACP2.1 Q3ACP2_CARHZ Putative uncharacterized protein 251 1e-64 A1HLP5.1 A1HLP5_9FIRM Type III effector Hrp-dependent outers 215 7e-54 >A4ITC0.1 A4ITC0_GEOTN Putative uncharacterized protein Length = 430 Score = 296 bits (758), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 178/455 (39%), Positives = 261/455 (57%), Gaps = 36/455 (7%) Query: 1 MNFAVIADDLTGANDTGVQMARQGLATSVLLELKTGGGK-QEAVVFDTDSRSLPPLEAYQ 59 M A+IADDLTGAND+G Q A GL TSVL + AVV DTD+RSLP +AY+ Sbjct: 1 MKIAIIADDLTGANDSGAQFAYHGLNTSVLFQPDPNAWHLSNAVVIDTDTRSLPKEKAYE 60 Query: 60 KVKDVCLFLGRRGEASSPVIYKKFDSTLRGNIGSELDAVYETLQPDFVVIAPSFPRVGRT 119 KVK V + + + ++YKK DSTLRGNIG+E+DA+Y+TLQP F++IAP++P+ GRT Sbjct: 61 KVKSVSETIKKY----ATLVYKKIDSTLRGNIGAEIDAIYDTLQPTFMMIAPAYPQNGRT 116 Query: 120 LADGIVYVNGMPLHETEMACDPKHPATESAFSAILERQSRYPAALISLEQLRGERAALDE 179 + G +++G LHETE++ DPK P ES+ +L +Q++ P +I + LR + E Sbjct: 117 VYQGYHFLHGTLLHETELSRDPKCPIDESSIPRLLAKQTKRPIGVIDYQTLRLGFDKVKE 176 Query: 180 LIAGYRQSGIPYLVFDAEREEDLALIASLFPEGPGRPKVVWXXXXXXXXXXXXQILRSLR 239 + YR GIPY+VFD+ E+DL IA + +VVW + L+ Sbjct: 177 QLDHYRADGIPYIVFDSVSEDDLRDIAQYVRQ--LNEQVVWVGSAGLAYYLPE--VYDLQ 232 Query: 240 EAPEQAGVVLNKSGNKQAASCDNVVYYDTNCVLIVVGSVNPWSRRQLTELVKEDSVSAIV 299 +P+ + NK +L+VVGSV+ SR QL ++ +V I Sbjct: 233 TSPKTMIIKPNK-----------------RPILLVVGSVSQVSRTQLDRVLSLPNVKGIQ 275 Query: 300 VESDKLLFPSSCEMELNRVRELVTGIIS-----REGKHVALYTSGDPDEVDRVRKLGRAS 354 V+S +L+ C+ + +R+ E+ + ++G H+ LY+S ++ RVR +G A Sbjct: 276 VDSVQLV----CDHQ-SRITEMEKALRQAENEVKQGFHIVLYSSASSSDIQRVRAIGEAG 330 Query: 355 GLSATEVGKRISAALGRVAADIIAECGIRHMVLTGGDTAKQVCRHLSDVEIKLIDEVENG 414 G + E I+ +LG VAA++I MVLTGGDTAK VC + +LIDE+E G Sbjct: 331 GRTPIETSNLIAESLGEVAAELIRNGYFGAMVLTGGDTAKYVCSQIGATGFELIDEIEPG 390 Query: 415 VPLGRTPGESGRYIITKAGAFGSDQVLVRSVKALQ 449 VP+ + ES Y++TKAGAFGS+ L+++V LQ Sbjct: 391 VPIAKLIHESEMYVVTKAGAFGSENTLIQAVHQLQ 425 >B3K3L1.1 B3K3L1_9BACI Type III effector Hrp-dependent outer protein Length = 431 Score = 288 bits (736), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 164/453 (36%), Positives = 254/453 (56%), Gaps = 27/453 (5%) Query: 1 MNFAVIADDLTGANDTGVQMARQGLATSVLLELKTGGGKQ-EAVVFDTDSRSLPPLEAYQ 59 M A+IADDLTGAND+GVQ+AR GL TSVL ++ + +AVVFDTDSRS+ P EAYQ Sbjct: 1 MKLAIIADDLTGANDSGVQLARHGLKTSVLFDMDQDNIRHYDAVVFDTDSRSISPEEAYQ 60 Query: 60 KVKDVCLFLGRRGEASSPVIYKKFDSTLRGNIGSELDAVYETLQPDFVVIAPSFPRVGRT 119 +V FL +A I+KK DST+RGNIG+E+DA+Y+ ++PDF++IAP +P+ RT Sbjct: 61 RVSRAAQFLT---DAGFDTIFKKMDSTMRGNIGTEIDALYDVVKPDFMMIAPGYPKNNRT 117 Query: 120 LADGIVYVNGMPLHETEMACDPKHPATESAFSAILERQSRYPAALISLEQLRGERAALDE 179 + + Y+NG+PL ETE+A DPK P T S +L+ Q++Y IS+ L ++ Sbjct: 118 ILNATHYLNGVPLAETEIAHDPKTPVTHSYLPDLLKLQTKYAVGEISVRDLEAGAGRIEG 177 Query: 180 LIAGYRQSGIPYLVFDAEREEDLALIASLFPEGPGRPKVVWXXXXXXXXXXXXQILRSLR 239 + +++ IPY++ D+ E L I + + P K W + Sbjct: 178 KLKEFQEHNIPYVLIDSTEEAHLEFILHITGQLP--YKFAWAGSAGIANYLPAHY--HIS 233 Query: 240 EAPEQAGVVLNKSGNKQAASCDNVVYYDTNCVLIVVGSVNPWSRRQLTELVKEDSVSAIV 299 P + + N+ +L VVGSVN SR QL L++ V + Sbjct: 234 SKPRELAIAQNQG-----------------PILTVVGSVNKNSRAQLKLLLEHTDVVPVH 276 Query: 300 VESDKLLFPSSCEM-ELNRVRELVTGIISREGKHVALYTSGDPDEVDRVRKLGRASGLSA 358 ES K + S+ E++RV + V + + EGK + +Y++ + +++R R +G A GL+ Sbjct: 277 FESYKAVSDSAERTEEMDRVYQEVK-LHALEGKDIVIYSTAESSDIERARAVGEARGLNH 335 Query: 359 TEVGKRISAALGRVAADIIAECGIRHMVLTGGDTAKQVCRHLSDVEIKLIDEVENGVPLG 418 TEV I A+G + A ++ + + + +TGGDTAKQ+C S +L+DE+E GVP+ Sbjct: 336 TEVSNEIVRAMGEICAQLLEQGYFKGVSMTGGDTAKQICMQWSISGFELLDELEIGVPIS 395 Query: 419 RTPGESGRYIITKAGAFGSDQVLVRSVKALQKG 451 + G Y++TKAG FG V + +++ ++ G Sbjct: 396 KFLGIDDLYVVTKAGGFGKPDVFIHAIQTIKGG 428 >Q65EJ4.1 Q65EJ4_BACLD Putative uncharacterized protein Length = 423 Score = 258 bits (660), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 171/455 (37%), Positives = 242/455 (53%), Gaps = 36/455 (7%) Query: 1 MNFAVIADDLTGANDTGVQMARQGLATSVLLELKTGGGKQEAVV-FDTDSRSLPPLEAYQ 59 M A+IADDLTGAND G Q+ G+ SV L+ + + E VV F+TDSRSLP EA Sbjct: 1 MKIAIIADDLTGANDCGGQLVHYGMDVSVKLDAEFTRRETEGVVIFNTDSRSLPAAEAKA 60 Query: 60 KVKDVCLFLGRRGEASSPVIYKKFDSTLRGNIGSELDAVYETLQPDFVVIAPSFPRVGRT 119 VK++ + R+ S ++YKK DST+RGNIG+EL A+YE QPDFV+IAP +P+ GR Sbjct: 61 TVKNISEHISRQ---SFDIVYKKIDSTMRGNIGAELQAMYEVFQPDFVLIAPGYPQNGRQ 117 Query: 120 LADGIVYVNGMPLHETEMACDPKHPATESAFSAILERQSRYPAALISLEQLRGERAALDE 179 + GI YVNG+ L ETE A DPK P ES ++E+Q+R + +S E +R + Sbjct: 118 VIGGIHYVNGVKLEETEAANDPKTPVAESNIQRLIEKQTRNKSGHLSYEDIRRGHGHVRN 177 Query: 180 LIAGYRQSGIPYLVFDAEREEDLALIASLFPEGPGRPKVVWXXXXXXXXXXXXQILRSLR 239 + +R GI Y+ D+ E DL +A + P IL Sbjct: 178 KLERFRAEGISYVTADSVHESDLQGLALILESLP-----------------YSIILAG-- 218 Query: 240 EAPEQAGVV--LNKSGNKQAASCDNVVYYDTNCVLIVVGSVNPWSRRQLTELVKEDSVSA 297 AG++ L K+ N + V ++ VVGS++ R QL EL + Sbjct: 219 ----SAGLMSCLPKAFNLKERELGQTVPSSDQPIMFVVGSMSRTGREQLHELFAGRAAEK 274 Query: 298 IVVESDKLLFPSSCEM-ELNRVRELVTGIISREGKHVALYTSGDPDEVDRVRKLGRASGL 356 I + S+++L + + E R++E + R KH+ L TS D +K+G + GL Sbjct: 275 IEMRSERVLCGGAEKRNEFERLKERAAD-VQRRSKHIVLCTS---DNAAETQKIGESRGL 330 Query: 357 SATEVGKRISAALGRVAADIIAECGIRHMVLTGGDTAKQVCRHLSDVEIKLIDEVENGVP 416 + + +S ALG A ++I ++ + LTGGDTA QV L EI+L+DEVE G+P Sbjct: 331 TPVQTSNAVSGALGEFAGELIQAFHVKRLFLTGGDTAYQVLHQLRIHEIRLLDEVEPGIP 390 Query: 417 LGRTPGESGRYIITKAGAFGSDQVLVRSVKALQKG 451 LG GE YI+TKAG FGS V+ + LQ G Sbjct: 391 LG-AAGEE-LYIVTKAGNFGSRSVMASAAIKLQGG 423 >Q3ACP2.1 Q3ACP2_CARHZ Putative uncharacterized protein Length = 432 Score = 251 bits (641), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 148/451 (32%), Positives = 256/451 (56%), Gaps = 29/451 (6%) Query: 3 FAVIADDLTGANDTGVQMARQGLATSVLLELKT---GGGKQEAVVFDTDSRSLPPLEAYQ 59 A+IADDLTGA+DTGVQ ++GL T V+ ++K VV DTDSRSL AYQ Sbjct: 4 IAIIADDLTGASDTGVQFTQKGLRTLVIFDIKNLFEDAKNVNVVVIDTDSRSLTSSAAYQ 63 Query: 60 KVKDVCLFLGRRGEASSPVIYKKFDSTLRGNIGSELDAVYETLQPDFVVIAPSFPRVGRT 119 K+K+V L +G +IYKK DSTLRGNIG+E+DA+ + + DF I P++P++GRT Sbjct: 64 KIKEVSQVLKPKG---YKLIYKKIDSTLRGNIGAEIDAIMDVITFDFAAIVPAYPKMGRT 120 Query: 120 LADGIVYVNGMPLHETEMACDPKHPATESAFSAILERQSRYPAALISLEQLRGERAALDE 179 +G +++G+P+ +TE+A DP P +S ++L QS+ L++L+ +R + Sbjct: 121 TIEGQHFLHGVPIDKTEIANDPICPVKDSNIVSLLSSQSKRNIGLVNLQTIRSGIENIKA 180 Query: 180 LIAGYRQSGIPYLVFDAEREEDLALIASLFPEGPGRPKVVWXXXXXXXXXXXXQILRSLR 239 + + + +VFD+E +DL ++ +++W + SL Sbjct: 181 KVDNLLKKDVQLIVFDSETSQDLKIVTEYL--SSSNYQILWVGSAG--------LAESLL 230 Query: 240 EAPEQAGVVLNKSGNKQAASCDNVVYYDTNCVLIVVGSVNPWSRRQLTELVKEDSVSAIV 299 +A E N + S + N VL+V+GS++ +++QL+ ++K+ +V+++ Sbjct: 231 DALE---FTFNDISQFKLLSTE-------NPVLLVIGSISKITQQQLSIILKQPNVTSVK 280 Query: 300 VESDKLLFPSSCEMELNR-VRELVTGIISREGKHVALYTSGDPDEVDRVRKLGRASGLSA 358 ++ +L E++R + E+ + ++G VA + + P+ V + +++G+ + Sbjct: 281 LDPLNVLKDEQYNNEISRCLVEIKLAL--KKGFDVAFHVNSSPEIVQKTQEIGKLLNIPQ 338 Query: 359 TEVGKRISAALGRVAADIIAECGIRHMVLTGGDTAKQVCRHLSDVEIKLIDEVENGVPLG 418 ++ RI+ ALG +++ +I E ++ ++LTGGDTAK VC+HL +KLI E+E GVP+ Sbjct: 339 NQISNRIADALGIISSQVIKEIPLQGLILTGGDTAKSVCKHLGVRGLKLIKELEPGVPIS 398 Query: 419 RTPGESGRYIITKAGAFGSDQVLVRSVKALQ 449 + G ITKAGAFG+++ L+ +V L+ Sbjct: 399 QLIGFGNLLAITKAGAFGTERTLINAVHFLK 429 >A1HLP5.1 A1HLP5_9FIRM Type III effector Hrp-dependent outers Length = 422 Score = 215 bits (548), Expect = 7e-54, Method: Compositional matrix adjust. Identities = 155/455 (34%), Positives = 236/455 (51%), Gaps = 43/455 (9%) Query: 2 NFAVIADDLTGANDTGVQMARQGLATSVLLELKTGGGKQ-EAVVFDTDSRSLPPLEAYQK 60 AVIADDLTGANDTGVQ A+QGL VLL + Q + +V DT+SR++ EAY++ Sbjct: 3 KLAVIADDLTGANDTGVQFAKQGLTVEVLLGSQDAAATQADIIVIDTNSRAISSAEAYRR 62 Query: 61 VKDVCLFLGRRGEASSPVIYKKFDSTLRGNIGSELDAVYETLQPDFVVIAPSFPRVGRTL 120 V + G A+ +YKK DSTLRGN+G+E+DA+ +T ++AP++P+ GR + Sbjct: 63 VAQAAAIVKASGIAA---VYKKIDSTLRGNLGAEIDAIMDTCGQAAAIVAPAYPQNGRCV 119 Query: 121 ADGIVYVNGMPLHETEMACDPKHPATESAFSAILERQSRYPAALISLEQ-LRGE---RAA 176 G +N +PL TE+A DP+ P ES ++ Q+R P +S + L+GE +AA Sbjct: 120 IGGYHLLNNIPLEATEIARDPRCPVKESHIPTLIAEQTRRPVGHLSFKTLLKGEDATQAA 179 Query: 177 LDELIAGYRQSGIPYLVFDAEREEDLALIASLFPEGPGRPKVVWXXXXXXXXXXXXQILR 236 L+ELI G +V DA E+ + + G+P V+W Q L Sbjct: 180 LEELIG----CGKEVIVCDA-WEDKQLALIAAAAANLGKP-VLW-----VGSAGLAQWLP 228 Query: 237 SLREAPEQAGVVLNKSGNKQAASCDNVVYYDTNCVLIVVGSVNPWSRRQLTELVKEDSVS 296 + VL + KQ++ VL++ GSV+ +R Q+ +L + +V Sbjct: 229 T----------VLGLTARKQSSP-----------VLVIAGSVSGVTRGQVAKLKQNANVK 267 Query: 297 AIVVESDKLLFPSSCEMELNRVRELVTGIISREGKHVALYTSGDPDEVDRVRKLGRASGL 356 I V++ LL + E+ R + REG V + + + V+ + G A G Sbjct: 268 GIEVDARALLAEPTRSGEVKRCCAEAAAAL-REGYDVVIASGYAEEVVEHTKAQGAALGY 326 Query: 357 SATEVGKRISAALGRVAADIIAECGIRHMVLTGGDTAKQVCRHLSDVEIKLIDEVENGVP 416 +A +VG ++ ALG + + +E + MVLTGGD A VCR L + + EV G+P Sbjct: 327 TAQQVGDIVAGALGEIGKGLASELTLAGMVLTGGDIAVSVCRALGAASLSVASEVAPGIP 386 Query: 417 LGRTPGE--SGRYIITKAGAFGSDQVLVRSVKALQ 449 +G G G ++TKAGAFG + LV++V L+ Sbjct: 387 VGFLTGGLCDGLKVVTKAGAFGGEDALVKAVHCLK 421 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,554,193,849 Number of extensions: 104896879 Number of successful extensions: 300740 Number of sequences better than 1.0e-05: 445 Number of HSP's gapped: 300036 Number of HSP's successfully gapped: 516 Length of query: 453 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 314 Effective length of database: 1,751,175,118 Effective search space: 549868987052 Effective search space used: 549868987052 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)