BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0008.p (593 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4ITB8.1 A4ITB8_GEOTN Sigma-L-dependent transcriptional regulator 381 e-103 C1PBS8.1 C1PBS8_BACCO Proprionate catabolism activator, Fis family 237 2e-60 B1DGN6.1 B1DGN6_9BACL Proprionate catabolism activator, Fis family 234 3e-59 A8FHB1.1 A8FHB1_BACP2 Transcriptional regulator 227 2e-57 Q65EJ1.1 Q65EJ1_BACLD Putative uncharacterized protein 222 8e-56 >A4ITB8.1 A4ITB8_GEOTN Sigma-L-dependent transcriptional regulator Length = 593 Score = 381 bits (978), Expect = e-103, Method: Compositional matrix adjust. Identities = 214/600 (35%), Positives = 348/600 (58%), Gaps = 28/600 (4%) Query: 11 MKIKTIVIAPYPGLSELTLSLQNELLEFEIVVEHGDLSRVLPLIGRIHTEGYDVIISRGG 70 M IKT++IAPYPGL EL +L E E ++ V GDL +P++ + EG+D++ISRGG Sbjct: 3 MNIKTLLIAPYPGLKELASTLAKEQQELDLTVVQGDLQEAIPIVKQYEKEGFDLVISRGG 62 Query: 71 TAKLLQKHSYLPVVEIPISGFDILRILTLVKGYSAKCEMIGFPNIIEGFMSVSSLMEVDI 130 TA+LL++++ LPV+EI +SG+DILRILTLVK Y A +IGF NI +GF+SVS+L+++ I Sbjct: 63 TAQLLRQYTSLPVIEIQVSGYDILRILTLVKDYHAHHWIIGFTNICQGFVSVSNLLDIQI 122 Query: 131 SYTVIHQEKEVGAALADAKARGIQVVIGDSITVKMAAENGLQGVLITSGKESLMEAFSRA 190 YT+IH +KEV A+ +AKA G Q +IGD++T + A +GLQ +LITSGKES++EAF +A Sbjct: 123 PYTIIHDQKEVDEAVKEAKAAGAQTIIGDTVTCRTAEAHGLQSILITSGKESVIEAFEQA 182 Query: 191 GQLYKSAEKLKIKNEMYEAMLGKLQGGYAVADQGGKLEFANPLFK---QLLRLPETADTL 247 Q Y+ + + + Y+ +L + + AV ++ G +E+A+P F+ ++ ++ Sbjct: 183 KQTYQMLKNTAKEVKRYKQLLHQSRVAIAVYNEQGTIEYASPAFQNRFNWMKNNSNESSI 242 Query: 248 YTMYPGLREMLRDAGRG-ADFDRATSVYEPEQGLYLRAQLL---PAADERRLYMLTASEE 303 Y +P L + R A + ++ ++ ++LR L P + + ++E Sbjct: 243 YACFPFLDHEYNELCRAIAPVEIQSTCIVNDKRMHLRQGCLNKDPLEPHYYIRLAESTEL 302 Query: 304 EPEHSGLAA---------SYLLAEAPFFSHQEEDGQKTDFQAYPIAVYGEKGSGRKRY-- 352 + EH + S ++ + E G++ P+A+YGE+GSG+K Sbjct: 303 QEEHDLTVSAVQPTMRSFSQIIGNSESIHRVIEQGKRAAMHREPVALYGEQGSGKKTLAG 362 Query: 353 VINAALSESRDGILYLNIRRSSEEVLKAMMELMLYGGGRVTAIEGAEMLSAKQQRELAEF 412 +I+ ++ +L++ I ++++ + + Y G ++G E+L +Q E+++ Sbjct: 363 IIHCEGKQTGGMLLHVRITADDDQLIDHLHSIFTYREGGTCVLQGVELLPLLRQDEISQL 422 Query: 413 IIKRKMKTVFLFDRDPGVLAEEERLAGKLLRSFQNRRISLPALRD-TPWLERSIRACLIW 471 I ++ +FLF+ DP LA +++L+ L + F+ +I LP+L + T LE+ R + Sbjct: 423 IATAVVRIIFLFENDPAHLARQKQLSHHLFKQFKANQIRLPSLSEYTEDLEKYARKFIAQ 482 Query: 472 TNERQGKQIAGIRGEVMDALLAHPWQGNFTELSTVMELFVSAAEGPFIEREALAMLEEGS 531 N + GKQ+ GI + L H W+GN EL+ VME + A +I+ E L + Sbjct: 483 YNVKYGKQVVGIDEAALKWLHRHHWKGNLQELNKVMEQAIQIARHEYIQVEDLKTI---- 538 Query: 532 RKARSGLAPAPGREGLNLRQPLVDIERDIIRTVLEQEGMNQSLAAKRLGINRSTLWRKLK 591 G + L+L +PL +IE++II VLE+E MNQ+ AAKRLGINRSTLWRKLK Sbjct: 539 -----GFLDSGNDLCLDLHKPLAEIEKEIIWKVLEEENMNQTRAAKRLGINRSTLWRKLK 593 >C1PBS8.1 C1PBS8_BACCO Proprionate catabolism activator, Fis family Length = 593 Score = 237 bits (605), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 194/623 (31%), Positives = 310/623 (49%), Gaps = 77/623 (12%) Query: 12 KIKTIVIAPYPGLSELTLSLQNELLEFEIVVEHGDLSRVLPLIGRIHTEGYDVIISRGGT 71 KIK +VI PY GL E+ +Q E+ +F +E G+L + L +GY VIISRGGT Sbjct: 5 KIKALVIVPYEGLYEMMKEVQREVGDFHFDIELGNLFEGVALAKEAENKGYHVIISRGGT 64 Query: 72 AKLLQKHSYLPVVEIPISGFDILRILTLVKGYSAKCEMIGFPNIIEGFMSVSSLMEVDIS 131 A ++Q+ +PV++I +SG+DILRI+TLVKG+S K ++GF NI +G +VS L+++DI Sbjct: 65 ASMIQEAVSIPVIDIRVSGYDILRIITLVKGFSRKAAIVGFSNITQGASTVSQLLDLDIE 124 Query: 132 YTVIHQEKEVGAALADAKARGIQVVIGDSITVKMAAENGLQGVLITSGKESLMEAFSRAG 191 I + EV L K+RG +VVIGD +T + A + G+ GVLITSGKE++MEA A Sbjct: 125 TMTITKGTEVNEKLLYLKSRGYEVVIGDVVTFQAAKKLGMTGVLITSGKEAIMEALEEAR 184 Query: 192 QLYKSAEKLKIKNEMYEAMLGKLQGGYAVADQGGKLEFANPLFKQLLRL--PETADTLY- 248 + Y+ KL+ + +++ +L + + ++ K+ +AN F + E+++ + Sbjct: 185 RSYRIFSKLQQQVSLFQFVLDCNREAIGIFNKEKKIVYANQRFNKEFHWMTVESSEEIKK 244 Query: 249 ----TMYPGLREMLRDAGRGADFDRA---TSVYEPEQGLYLRAQLLPAADERRLYMLTAS 301 T+ G +M R +++ + E + L +L A + S Sbjct: 245 LIQETVVAGESQM-----RTVHLNKSLWNITACPQEDTIVLLFELGKA--------IQTS 291 Query: 302 EEEPE--------HSGLAASYLLAEAPFFSHQEEDGQKTDFQA--YPIAVYGEKGSGRKR 351 +EE H+ + SY+ QE Q ++ P+ + GEKG G K Sbjct: 292 KEEGNIGRNAIEFHTSI--SYIPIAGKSAGIQEVQQQVAEYSKSEQPVWICGEKGVG-KD 348 Query: 352 YVINAALSESRDG---ILYLNIRRSSEEVLKAM-MELML--YGGGRVTAIEGAEMLSAKQ 405 ++ R G + +N E LK + +E Y G + ++ E L Sbjct: 349 TAARLLYTKRRAGTEPFIVINCDTIKAEQLKGLKVEEFFDKYTNGTI-YLKNIERLDKSL 407 Query: 406 QRELAEFIIKRKMKTVF---------------LFDRDPGVLAEEERLAGKLLRSFQNRRI 450 QRE + + K + LFDRD +L G+L +I Sbjct: 408 QREFCQIFKNKNNKLKWLASYEGKIEDKIKKGLFDRDL------YQLLGQL-------KI 454 Query: 451 SLPALRDTPW-LERSIRACLIWTNERQGKQIAGIRGEVMDALLAHPWQGNFTELSTVMEL 509 +P LR+ +E ++ + + + G Q+ G+R + + L+A+ W GN +L V+E Sbjct: 455 CIPPLRERKEDIEDLVQIFISELHPKHGNQVVGVRPDGLKELIAYDWPGNVKQLKQVIEQ 514 Query: 510 FVSAAEGPFIER-EALAMLEEGSRKARSGLAPAPGREGLNLRQPLVDIERDIIRTVLEQE 568 A+ +IE+ E + +L+ ++ P ++L L DIE+ II+ VLE+E Sbjct: 515 LFIQAKSFYIEKDEVVTVLKRLRESEQTDKTLIP----IDLSGTLEDIEKRIIKKVLEEE 570 Query: 569 GMNQSLAAKRLGINRSTLWRKLK 591 +NQS AAKRLGINRSTLWRKLK Sbjct: 571 ELNQSKAAKRLGINRSTLWRKLK 593 >B1DGN6.1 B1DGN6_9BACL Proprionate catabolism activator, Fis family Length = 617 Score = 234 bits (596), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 188/624 (30%), Positives = 299/624 (47%), Gaps = 55/624 (8%) Query: 11 MKIKTIVIAPYPGLSELTLSLQNELLEFEIVVEHGDLSRVLPLIGRIHTEGYDVIISRGG 70 MKIK + IAPY GL +L L + E + VE DL LPLI + GYD+IISRGG Sbjct: 1 MKIKVLAIAPYVGLRDLLLEMVQEETMIRMDVEVADLEEALPLIQQAEERGYDIIISRGG 60 Query: 71 TAKLLQKHSYLPVVEIPISGFDILRILTLVKGYSAKCEMIGFPNIIEGFMSVSSLMEVDI 130 TA L++KHS LPVV+IP+SG+DILR+LTL++ ++K +IGFPNI + VSSL++ +I Sbjct: 61 TANLIRKHSSLPVVDIPVSGYDILRVLTLIRRTNSKVAIIGFPNICKAVAEVSSLLDFEI 120 Query: 131 SYTVIHQEKEVGAALADAKARGIQVVIGDSITVKMAAENGLQGVLITSGKESLMEAFSRA 190 I + EV AL A +Q+V+GD++TV+ A E G G+LITSGKES+ + F Sbjct: 121 PIIQISDQTEVRGALRQAFGDKVQIVLGDAVTVRAAQEMGYHGILITSGKESVGDMFREV 180 Query: 191 GQLYKSAEKLKIKNEMYEAMLGKLQGGYAVADQGGKLEFANPLFKQLLRLPETAD----- 245 ++Y + + + Y +L D ++ F N +LL A+ Sbjct: 181 RRVYDVYRQGQEQTMFYRDILDHDARAIIALDDRHRILFINRTAGELLGSSGNAEADALP 240 Query: 246 --TLYTMYPGLREMLRDAGRGADFDRATSVYEPEQGLYLRAQLLPAADE--------RRL 295 ++ P L +++ A + + + +Q LYL Q + R L Sbjct: 241 GSSIQYSLPALYKLINGAEEADNAAKWS-----KQFLYLNEQQYKVSVSEGRKEGSFRYL 295 Query: 296 YMLTASEEEPEHSGLAA---------SYLLAEAPFFSHQEEDGQKTDFQAYPIAVYGEKG 346 L + E AA + ++ + + QK + V GE+G Sbjct: 296 VSLDSIENWRNERRFAAYIPPRLTTFNQMVGSSVMLNKTIAKAQKYALSDRNLWVSGERG 355 Query: 347 SGRKRY--VINAALSESRDGILYLNIRRSSEE----VLKAMME---LMLYGGGRVTAIEG 397 +G+ + I++ G + +E+ +LK E L G G + Sbjct: 356 TGKSLFAQAIHSGSKRHAQGFYMIPCEGMTEQETNLLLKGTAEELGLPETGFGGTIYLNN 415 Query: 398 AEMLSAKQQRELAEFIIKRKMKTVFLFDRDPGVLAEEERLAGK------LLRSFQNRRIS 451 + L Q + + I+R + ++ + RL GK L+ + +++ Sbjct: 416 VDRLDRGAQDQWLQ-AIRRYPQIRYIASSSTSL----TRLKGKADYNHELIAALGELQLA 470 Query: 452 LPALRD-TPWLERSIRACLIWTNERQGKQIAGIRGEVMDALLAHPWQGNFTELSTVMELF 510 +P L++ ++ IR + N + GKQI G R +D LL + W GN EL + Sbjct: 471 VPPLKERMEDIDEIIRVMIANYNFQSGKQIVGFRETALDELLRYDWPGNLRELERAIHEM 530 Query: 511 VSAAEGPFIE-REALAMLEEGSRKARSGLAPAPG-REGLNLRQPLVDIERDIIRTVLEQE 568 + +G +E ++A+ ++E+ S +A A ++LR L +IER II VL++E Sbjct: 531 LVLTQGNHVEKKDAVTVIEQ---YKESLIADADNVSTTIDLRGTLEEIERRIITYVLQEE 587 Query: 569 GMNQSLAAKRLGINRSTLWRKLKE 592 GMNQS KRLG+NR+T WRK+ + Sbjct: 588 GMNQSKTCKRLGMNRTTFWRKMNK 611 >A8FHB1.1 A8FHB1_BACP2 Transcriptional regulator Length = 593 Score = 227 bits (579), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 183/616 (29%), Positives = 289/616 (46%), Gaps = 57/616 (9%) Query: 11 MKIKTIVIAPYPGLSELTLSLQNELLEFEIVVEHGDLSRVLPLIGRIHTEGYDVIISRGG 70 M I + +APY GL ++ + L E +E GDL + L + EG D+I+SRGG Sbjct: 1 MNINVLGVAPYKGLGDVLIDLAKEEKNITFQLEVGDLRSGVALAEQAEAEGMDIIMSRGG 60 Query: 71 TAKLLQKHSYLPVVEIPISGFDILRILTLVKGYSAKCEMIGFPNIIEGFMSVSSLMEVDI 130 TA L+Q+H +PVV+IP+SG+D+LR LTL+K Y + ++GF NI +G ++ L + I Sbjct: 61 TAALIQQHVNIPVVDIPVSGYDLLRALTLIKDYHGQKAVVGFENITQGVRTIGDLYGIKI 120 Query: 131 SYTVIHQEKEVGAALADAKARGIQVVIGDSITVKMAAENGLQGVLITSGKESLMEAFSRA 190 I QE+EV L + +G +VV+GD IT K A E G+Q +LITSG+ES+ EAF +A Sbjct: 121 DIYTIQQEEEVWDLLKQLQEQGTKVVLGDVITDKAAKELGMQSMLITSGRESVKEAFHQA 180 Query: 191 GQLYKSAEKLKIKNEMYEAMLGKLQGGYAVADQGGKLEFANPLFKQLLR-------LPET 243 Q+Y+ ++ + ++ M+ + G + + +L F N K + P Sbjct: 181 KQMYRLYKEATAEQRLFREMIDQAPKGMLIINPQNQLYFMNQQMKHWMEKGLFAPFFPH- 239 Query: 244 ADTLYTMYPGLREMLRDAGRGADFDRATSVYEPEQGLYLRAQLL-----------PAA-- 290 D + P L ++ A R + + + +Q +++ +L P+ Sbjct: 240 -DDVIAWCPALEPAIK-AIREGKQNGFSQISHQQQEWHVKGNILSKGELLVTIEKPSTVM 297 Query: 291 --DERRLYMLTASEEEPEHSGLAASYLLAEAPFFSHQEEDGQKTDFQAYPIAVYGEKGSG 348 ++ R++ L + P HS S+ ++P + + + I +YGE+GSG Sbjct: 298 NREDHRIWTLAS----PVHSEHPFSFFTEQSPKMQEVIKRAKAFSQTSRSICLYGEQGSG 353 Query: 349 RKR--YVINAALSESRDGILYLNIRRSSEEVLKAMMELMLYGGGRVTAIEGAEM---LSA 403 + + I+ + ++ R +E + L G + IE M L Sbjct: 354 KSSLAFAIHQMSERKNESFTTVDCRHITEPDDVRSIPLYQKGTMFLKDIECVPMELQLLI 413 Query: 404 KQQRELAEFIIKRKMKTVFLFD-RDPGVLAEEERLAGKLLRSFQNRRISLPALRDTPW-L 461 Q + I TV LF G +EE L Q I +P L + P + Sbjct: 414 AGQIHHHQNIRWIAGTTVDLFKLMQSGTFSEE------LYTCLQELTIHVPPLSERPEDI 467 Query: 462 ERSIRACLIWTNERQGKQIAGIRGEVMDALLAHPWQGNFTELSTVM-ELFVSAAEGPFIE 520 E R + N G Q+ G+ +V++A ++ N + ++ EL ++ G Sbjct: 468 EDISRLFIAELNSLYGTQVVGLSVDVIEAFQHKKFRENVRQFKRIIEELVLTTKSGYITL 527 Query: 521 REALAMLE----EGSRKARSGLAPAPGREGLNLRQPLVDIERDIIRTVLEQEGMNQSLAA 576 EA A++ E K S L L +IER II+TVLE+E MNQS AA Sbjct: 528 NEAKAVMNQLAFENEEKQLSSY----------LNGTLDEIERRIIKTVLEEENMNQSKAA 577 Query: 577 KRLGINRSTLWRKLKE 592 KRL INR+TLWRKLKE Sbjct: 578 KRLNINRTTLWRKLKE 593 >Q65EJ1.1 Q65EJ1_BACLD Putative uncharacterized protein Length = 572 Score = 222 bits (566), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 186/610 (30%), Positives = 297/610 (48%), Gaps = 72/610 (11%) Query: 13 IKTIVIAPYPGLSELTLSLQN-ELLEFEIVVEHGDLSRVLPLIGRIHTEGYDVIISRGGT 71 +K + IAPY GL EL + + E EFE GDL + + L + G D+IISRGGT Sbjct: 1 MKVMAIAPYEGLKELMVQVAGGENFEFEAA--SGDLEKGVELAKQAEANGTDLIISRGGT 58 Query: 72 AKLLQKHSYLPVVEIPISGFDILRILTLVKGYSAKCEMIGFPNIIEGFMSVSSLMEVDIS 131 A+L+Q+ +PVV+I +SG+D+LR+LTL KGY K ++GFP I +G +V ++++ I Sbjct: 59 AELIQQEVSIPVVDIEVSGYDMLRVLTLAKGYPGKAAIVGFPMIADGASAVCEILDIRID 118 Query: 132 YTVIHQEKEVGAALADAKARGIQVVIGDSITVKMAAENGLQGVLITSGKESLMEAFSRAG 191 + E++V L K +G Q++IGD IT++ A + GL G+L+TSGKES+ +AF+ A Sbjct: 119 ACTVSDERDVEPVLLQLKEQGYQIIIGDVITIEKAEQLGLNGLLLTSGKESIAKAFAYAK 178 Query: 192 QLYKSAEKLKIKNEMYEAMLGKLQGGYAVADQGGKLEFANPLFKQLLRLPETADTLY--T 249 +LY E +K K + +L K + GYAV ++ + F N +F + + L + + L Sbjct: 179 KLYSFLESIKQKYAVPYHILQKAETGYAVYNKTLRPVFQNGVFAEHIGLLDNLEELIGEA 238 Query: 250 MYPGLREM-LRDAGRGADF--------DRATSVYEPEQGLYLRAQLLPAAD----ERRLY 296 G+ LR GR + D A +V+ E +R LP A R Y Sbjct: 239 EKKGVIHFRLRKHGRTWNITGRRFFVSDEALTVFTAENAKDVRVSSLPGATILSPLRMSY 298 Query: 297 MLTASEEEPEHSGLAASYLLAEAPFFSHQEEDGQKTDFQAYPIAVYGEKGSGRKRY--VI 354 M T+ E +L +A FS + E P+ + G GSG++ +I Sbjct: 299 METSRNE-------TMQKVLKKAELFSARRE----------PVWINGAPGSGKESLAGLI 341 Query: 355 NAALSESRDGILYLNIRRSSEEVLKAMMELMLYGGGRVT-AIEGAEMLSAKQQRELAEFI 413 + + + ++ SE + E + GR T I+ + + QR + Sbjct: 342 HMKGEDKHEPFRKIDAGLLSEAEWEQACESEEFFSGRGTLYIKQVDRMPPSSQR-----L 396 Query: 414 IKRKMKTVFLFDRDPGVLAEEER-----LAGKLLRSFQNRRISLPALRDTPWLERSIRAC 468 + R++ + P ++ R + L + + + +P L + E + Sbjct: 397 LLRRISEPY---DGPRLIVSSSRRRAEEFSSDLYDALCHLELDVPPLSERK--EDILHLA 451 Query: 469 LIW---TNERQGKQIAGIRGEVMDALLAHPWQGNFTELSTVMELFVSAAEGPFIER---- 521 ++ +NE GKQI G R E L W GN ++L ++ V A+G +IE+ Sbjct: 452 YLFIAESNEAFGKQIVGFRNEAGVLLEEAKWPGNLSQLRKTVQNCVLEAQGVYIEKTDAE 511 Query: 522 EALAMLEEGSRKARSGLAPAPGREGLNLRQPLVDIERDIIRTVLEQEGMNQSLAAKRLGI 581 L+ L E RE ++L L +IE+ II+ + +EG NQ+ A+RLGI Sbjct: 512 RVLSRLNEEE------------REDIDLSGTLEEIEKRIIQKIYAEEGNNQTRTAERLGI 559 Query: 582 NRSTLWRKLK 591 NR+TLWRKLK Sbjct: 560 NRTTLWRKLK 569 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,316,151,278 Number of extensions: 139121721 Number of successful extensions: 381928 Number of sequences better than 1.0e-05: 777 Number of HSP's gapped: 383552 Number of HSP's successfully gapped: 797 Length of query: 593 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 451 Effective length of database: 1,722,912,073 Effective search space: 777033344923 Effective search space used: 777033344923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)