BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0007.p (385 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WLJ1.1 Q5WLJ1_BACSK Alcohol dehydrogenase 440 e-122 A8FHB4.1 A8FHB4_BACP2 Alcohol dehydrogenase EC=1.1.1.1 418 e-115 Q65EJ3.1 Q65EJ3_BACLD Putative uncharacterized protein 410 e-113 B3KI04.1 B3KI04_9BACI Iron-containing alcohol dehydrogenase 396 e-108 B1K9V3.1 B1K9V3_BURCC Iron-containing alcohol dehydrogenase 365 4e-99 >Q5WLJ1.1 Q5WLJ1_BACSK Alcohol dehydrogenase Length = 389 Score = 440 bits (1132), Expect = e-122, Method: Compositional matrix adjust. Identities = 221/385 (57%), Positives = 281/385 (72%), Gaps = 3/385 (0%) Query: 3 QYFFQTARSIIAGCGSLERIVEHLQK-LGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGI 61 Y FQTA +IAG G +V HLQK + S E VL+V+QPF++ +T+ L+ G+ Sbjct: 4 HYQFQTADKLIAGIGVFTELVSHLQKAVPSVERVLIVTQPFVKKLDFYQVMTSSLEKAGL 63 Query: 62 AVHWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREM 121 + TDV+PEPT A +E + + + D +IG+GGGSVLD KLLSV+ +N+ +++++ Sbjct: 64 SYEVVTDVEPEPTEANIENIKERVRDSSYDVLIGLGGGSVLDSTKLLSVLLSNTCTIQDI 123 Query: 122 LGTDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXX 181 LGTDL+ PGIPTVLIPTTSGTGSEVTPNAIVT P++ELKIG+VSR+ Sbjct: 124 LGTDLVKEPGIPTVLIPTTSGTGSEVTPNAIVTFPDKELKIGIVSRHLLPVLVVLDPLLT 183 Query: 182 XGLPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAR 241 LP+ ITA+TGMDA+TH+LESFISNKANRFSDMFALESIR I+ + AYH G++ +AR Sbjct: 184 VSLPKPITAATGMDAFTHSLESFISNKANRFSDMFALESIRLIADGIQTAYHDGSNVEAR 243 Query: 242 EDMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERL 301 E MLIGS YGGMALT AGTAAVHA+AYP+GGKF V HGVANSMLLPHVM +N D IV+RL Sbjct: 244 EKMLIGSTYGGMALTAAGTAAVHAMAYPLGGKFKVTHGVANSMLLPHVMAYNMDAIVDRL 303 Query: 302 ALVAGAMGLQ--TGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQ 359 A VA MG+Q GA Q+ A+ V+++I+ WT E SIPQDLT FG +D+ +L++AA Q Sbjct: 304 AQVADPMGIQLNQGATSQQKAKAVVQKIKEWTAEFSIPQDLTEFGVREDDLEDLAIAAVQ 363 Query: 360 VTRLMGNNPKAMTLDDIGAVYGKLF 384 V RLM NNPK MT++D +Y KL Sbjct: 364 VKRLMDNNPKEMTVNDAIQIYKKLL 388 >A8FHB4.1 A8FHB4_BACP2 Alcohol dehydrogenase EC=1.1.1.1 Length = 390 Score = 418 bits (1074), Expect = e-115, Method: Compositional matrix adjust. Identities = 209/384 (54%), Positives = 270/384 (70%), Gaps = 2/384 (0%) Query: 3 QYFFQTARSIIAGCGSLERIVEHLQKLG--SFESVLLVSQPFLQSSGTLDEVTNLLKSGG 60 Y FQTA I++G SL+ + E L LG S+ +++QP + S G D + + L G Sbjct: 5 HYHFQTASHIVSGAHSLDLLGEKLDGLGLSGLTSIFILTQPSIVSLGYADHIKSELTKKG 64 Query: 61 IAVHWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVRE 120 I +T ++PEPT +EE++ + DAIIGIGGGSVLD K+L+V+KTN + Sbjct: 65 ITSEISTAIKPEPTEENIEEVFQTFLQGEHDAIIGIGGGSVLDAAKILAVLKTNDEPISA 124 Query: 121 MLGTDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXX 180 ++GT+L+ G+PT+LIPTTSGTGSEVTPNAIVT PE+ELKIG+VS + Sbjct: 125 LVGTNLVKRRGVPTILIPTTSGTGSEVTPNAIVTFPEKELKIGMVSPHLLPDLVILDPAL 184 Query: 181 XXGLPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKA 240 LP+ ITA+TGMDA+THALES+ISNKAN FSDMFALES+R IS S+ +AYHHG + KA Sbjct: 185 TLNLPKAITAATGMDAFTHALESYISNKANPFSDMFALESMRLISGSIQEAYHHGENMKA 244 Query: 241 REDMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVER 300 REDML+G+MYGGMALT AGTAAVHA+AYP+GGK+ + HGVANSMLLPHV FN D I++R Sbjct: 245 REDMLVGAMYGGMALTSAGTAAVHAMAYPLGGKYKISHGVANSMLLPHVTAFNADHIIDR 304 Query: 301 LALVAGAMGLQTGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQV 360 L VAG +G+ ++ AE V+ QI WT ++ IPQ L FG ++ED+P L+ AAS+V Sbjct: 305 LEQVAGVIGIAKTDAKRPLAEQVVRQIEAWTADLHIPQQLKAFGVSKEDVPALAQAASEV 364 Query: 361 TRLMGNNPKAMTLDDIGAVYGKLF 384 RLM NNPK M++ DI VY KL Sbjct: 365 RRLMDNNPKPMSISDIEQVYMKLL 388 >Q65EJ3.1 Q65EJ3_BACLD Putative uncharacterized protein Length = 388 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 209/385 (54%), Positives = 276/385 (71%), Gaps = 2/385 (0%) Query: 3 QYFFQTARSIIAGCGSLERIVEHLQKL-GSFESVLLVSQPFLQSSGTLDEVTNLLKSGGI 61 Y F+TA I+AG S+ R+ +H++ + + L+++QP + G + EV L GI Sbjct: 4 NYQFRTAGHIVAGEHSIRRLKDHVRTIVPKADRALIITQPSIVKLGAIGEVQAQLTEIGI 63 Query: 62 AVHWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREM 121 T + PEPT+ +E+++ I E D +IGIGGGSVLD K+LSV++TNS V E+ Sbjct: 64 QSDVCTAILPEPTVQNIEDVFRGIREKRYDILIGIGGGSVLDGTKILSVLQTNSKKVEEL 123 Query: 122 LGTDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXX 181 LGTDL+ PGIPTVLIP+TSGTG+EVTPNAIVTLPEEELK+G+VS Sbjct: 124 LGTDLVEKPGIPTVLIPSTSGTGAEVTPNAIVTLPEEELKVGIVSPLLLPKLVILDPVIT 183 Query: 182 XGLPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAR 241 GLP+ ITA+TGMDA+TH+LESFIS KAN SDMFALESIR ISAS+++AY +G+ +AR Sbjct: 184 LGLPKPITAATGMDAFTHSLESFISTKANPISDMFALESIRLISASIVEAYENGSSIQAR 243 Query: 242 EDMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERL 301 E+ML+GS YGGMALT AGTAAVHALAYP+GGK+ + HGVANSMLLPHVMEFN D I ERL Sbjct: 244 ENMLLGSTYGGMALTAAGTAAVHALAYPLGGKYRISHGVANSMLLPHVMEFNMDAITERL 303 Query: 302 ALVAGAMGL-QTGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQV 360 +L A MG+ + ++ AE V+++IR WT ++IPQDL FG D+ +L+ +AS+V Sbjct: 304 SLAAETMGIVASDLTAEQAAEAVVQKIREWTERLNIPQDLKAFGVTASDVDDLADSASKV 363 Query: 361 TRLMGNNPKAMTLDDIGAVYGKLFH 385 TRL+ NNPK ++L++I +Y KL + Sbjct: 364 TRLLHNNPKPLSLENIKDIYRKLIN 388 >B3KI04.1 B3KI04_9BACI Iron-containing alcohol dehydrogenase Length = 403 Score = 396 bits (1017), Expect = e-108, Method: Compositional matrix adjust. Identities = 206/382 (53%), Positives = 258/382 (67%), Gaps = 1/382 (0%) Query: 4 YFFQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAV 63 Y FQT+ IIAG +++ + L+ LG L+V+ ++ G DE+ L GIA Sbjct: 8 YQFQTSGKIIAGLHAMQNLGPELRGLGRINKALIVASDAMRRQGATDELAARLAGSGIAS 67 Query: 64 HWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLG 123 V EPT +E L I + P D IGIGGGSVLD KLLSV++TN L VREM+G Sbjct: 68 ETIGGVMREPTADHIEALADRIGQEPFDVYIGIGGGSVLDATKLLSVLRTNRLRVREMIG 127 Query: 124 TDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXG 183 T+ IP+ G+PT LIPTTSGTGSEVTPNAIVTL EE LK+G+VS Sbjct: 128 TERIPHAGVPTALIPTTSGTGSEVTPNAIVTLEEEALKLGIVSSRLYASLVVLDPALTLS 187 Query: 184 LPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKARED 243 LPR ITA TGMDA+THALESF+SNKAN SDM ALESIR +S S++ A+H G+ + R Sbjct: 188 LPRSITAETGMDAFTHALESFLSNKANPISDMLALESIRLLSGSIVSAFHDGHAVEDRSA 247 Query: 244 MLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLAL 303 ML+GSM+GGMAL +GTAAVHA+AYP+GG+FH+PHG AN+MLLPHVM FN D + ++L Sbjct: 248 MLLGSMFGGMALAGSGTAAVHAMAYPLGGRFHIPHGAANAMLLPHVMAFNMDSVQDKLRR 307 Query: 304 VAGAMGLQTGAGRQRD-AEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTR 362 VA AMG+ T AG D AE V+E+I WT+E+ IPQ+L ++G D+P L+ AA QV R Sbjct: 308 VASAMGIVTEAGASADAAELVVERIAEWTKELQIPQNLAVYGVQSSDVPVLAEAAFQVKR 367 Query: 363 LMGNNPKAMTLDDIGAVYGKLF 384 LM NNPK M++ DI VY KL Sbjct: 368 LMDNNPKPMSILDIEDVYRKLL 389 >B1K9V3.1 B1K9V3_BURCC Iron-containing alcohol dehydrogenase Length = 388 Score = 365 bits (937), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 200/387 (51%), Positives = 266/387 (68%), Gaps = 3/387 (0%) Query: 1 MEQYFFQTARSIIAGCGSLERIVEHLQKLGS-FESVLLVSQPFLQSSGTLDEVTNLLKSG 59 M Y FQT R I+ G GSL + + L L + + + L++QP +++ G +D V L + Sbjct: 1 MTTYHFQTVRHIVHGAGSLATLPDKLALLDTPVKRIALITQPSMEALGIVDRVIESLDAK 60 Query: 60 GIAVHWNTDVQPEPTIAQLEELYS-AIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSV 118 + V V+PEPTI +E +++ IA AI+ IGGGSVLD KL +V TN + Sbjct: 61 DVDVRIIRGVEPEPTIGNVETVFNEQIAPFAPQAILSIGGGSVLDAAKLFAVRLTNDEPL 120 Query: 119 REMLGTDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXX 178 R LG DLI +PG+P +L+PTT+GTGSEVTPNAIVTLP+E LK+G+VSR+ Sbjct: 121 RNWLGIDLIKHPGVPMILVPTTAGTGSEVTPNAIVTLPDESLKVGIVSRHLLPQIVILDP 180 Query: 179 XXXXGLPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDR 238 GLP+ ITA+TGMDA+ HALES+IS KAN SDM+A+ES+R I A+++ AY +G Sbjct: 181 ELTLGLPKPITAATGMDAFVHALESYISTKANPISDMYAMESMRLIGANIVDAYENGQSL 240 Query: 239 KAREDMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIV 298 KARE M++GSMYGG+ALT AGTAAVHALAYP+GG F+V HGVAN+MLLPHVM FN D I Sbjct: 241 KAREAMMLGSMYGGLALTAAGTAAVHALAYPLGGMFNVTHGVANAMLLPHVMAFNLDAIE 300 Query: 299 ERLALVAGAMGLQTGAGRQRD-AEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAA 357 +RLA VA A+ L + AG D A +I+++ WT + IPQDL FG + + LS+AA Sbjct: 301 DRLANVARALDLASKAGTDADAARKLIDKMEEWTAAVDIPQDLRKFGVSEAHLDALSVAA 360 Query: 358 SQVTRLMGNNPKAMTLDDIGAVYGKLF 384 S+V RL+GNNPKA+TLDD+ ++Y +L Sbjct: 361 SKVKRLLGNNPKALTLDDMKSIYRRLL 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,130,038,864 Number of extensions: 85567621 Number of successful extensions: 242806 Number of sequences better than 1.0e-05: 3664 Number of HSP's gapped: 235037 Number of HSP's successfully gapped: 3668 Length of query: 385 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 248 Effective length of database: 1,770,017,148 Effective search space: 438964252704 Effective search space used: 438964252704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)