BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0003.p (428 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D8L0.1 B1D8L0_9BACL SNARE associated Golgi protein 441 e-122 C1PC56.1 C1PC56_BACCO SNARE associated Golgi protein 311 6e-83 A6LYE8.1 A6LYE8_CLOB8 SNARE associated Golgi protein 178 1e-42 B1HZT2.1 B1HZT2_LYSSC Hypothetical ybfM protein 152 6e-35 C3EFP4.1 C3EFP4_BACTK Alkaline phosphatase, DedA 151 1e-34 >B1D8L0.1 B1D8L0_9BACL SNARE associated Golgi protein Length = 398 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 219/394 (55%), Positives = 284/394 (72%) Query: 34 LMSYIGLSVYEQKLSWMLCIVSAGTGVLLGVTLSYWVGYRLGRPFIARYGARIHLTEERI 93 +MSY G+ V+E+KLSW+L I++A GV G+TLSYW+GY LG+PFIA+YG R H+ EE++ Sbjct: 1 MMSYAGMLVFEKKLSWLLSILTASAGVTTGITLSYWIGYWLGKPFIAKYGRRFHMGEEQM 60 Query: 94 VKMNRWFEKYGDKLLFVAYFIPGVRHITGYFCGVTRMPFRRYALFAYSGAIFWVSLFISL 153 ++ WFEKYGDKLLF+AYFIPGVRHITGYFCGVTRMPFRRYA++AYSGA+FWVSLFISL Sbjct: 61 ERLTVWFEKYGDKLLFIAYFIPGVRHITGYFCGVTRMPFRRYAIYAYSGAVFWVSLFISL 120 Query: 154 GKVLGPKWEQYHSTVNRYLIIFTVVSILLTAVIYLYRRYKHQVIETLMQLLIQGGRRFNS 213 GKVLGPKWE YHSTVNRYLIIF + S LL + L ++YK+++I L+ L+ G R S Sbjct: 121 GKVLGPKWEAYHSTVNRYLIIFAIASGLLFLLYNLLKKYKYRIISFLLDLVGGGVRHVRS 180 Query: 214 FGKVRXXXXXXXXXXXXXXXXXXGLIQDFLAQEFGLFDEIVNYLVAATFGTEWSGWMEAF 273 GK + G+IQD++AQEFG FDE+ YL G+ W ME F Sbjct: 181 LGKAKYLVLAAFAAFVLSLSLMFGMIQDYVAQEFGKFDEVTYYLTLMIIGSGWHDLMEDF 240 Query: 274 AKFGTLYFYGPVLGLTALWIVLHSKERRLDLTFLVWVVAGGELLDDMLRMVFHRPGPVTA 333 + G++Y Y PV+ LT + I + L++ F+ W +AGG +L++ LRMVFHR GPV++ Sbjct: 241 LQIGSIYLYAPVIALTLIVIRFKGNNKLLEIVFMCWTIAGGAVLNEGLRMVFHRTGPVSS 300 Query: 334 GVQLFNTFPSEETLISLTVCGFTAFLLLRYYSFRHIHTPIILGVILICFAVGISRIYFMV 393 G+ + NTFPSE+T ++TV GF FL+LR YS IH +I L+CF +GIS IYF + Sbjct: 301 GIPITNTFPSEDTFTAVTVLGFCVFLILRQYSQEIIHLVLIAAAALLCFLLGISLIYFNL 360 Query: 394 QYPSDVFAGYVFGGVWVSLNVMLLEILRKLQTVK 427 QYPSDV AGYVFGGVW SLNV++LE+ RKL+ + Sbjct: 361 QYPSDVAAGYVFGGVWASLNVLMLEVRRKLRRSR 394 >C1PC56.1 C1PC56_BACCO SNARE associated Golgi protein Length = 424 Score = 311 bits (798), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 166/422 (39%), Positives = 258/422 (61%), Gaps = 2/422 (0%) Query: 4 LTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGVLLG 63 +T ++ YGY ++ L L LEM+A+PLPGE+LMSY G V++ +L+W+L I+ A TG +G Sbjct: 3 VTSYVDQYGYIVLLLALLLEMIAVPLPGEVLMSYAGYMVHQGQLNWILSILIAATGTSVG 62 Query: 64 VTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHITGY 123 +T +Y++G RLG PF+ +YG IHL ++ K +RWF KYG+KLL VAYFIPGVRHITGY Sbjct: 63 MTTAYFIGQRLGGPFLHKYGHFIHLGPSQLEKTSRWFNKYGNKLLIVAYFIPGVRHITGY 122 Query: 124 FCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKWEQYHSTVNRYLIIFTVVSILLT 183 F G+T++PFR +A FAYSGA+ W S FISLGK+LGP+WEQ+H V +YL+I +VS L Sbjct: 123 FSGITKLPFRSFATFAYSGALIWTSTFISLGKLLGPQWEQFHEAVKKYLVIGGIVSAGLI 182 Query: 184 AVIYLYRRYKHQVIETLMQLLIQGGRRFNSFGKVRXXXXXXXXXXXXXXXXXXGLIQDFL 243 Y++R + Q+ +M +L + F S + +IQ+ Sbjct: 183 IAYYVFRYFHKQIFWAMMAVLRRVFMIFRSRVRAEFVIIGTTLVTLFLIVLMITMIQNLF 242 Query: 244 AQEFGLFDEIVNYLVAATFGTEWSGWMEAFAKFGTLYFYGPVLGLTALWIVLHSKERRLD 303 +++F F+E+ + +V+ F W+G M+ + V+ LT LWI+ K+R + Sbjct: 243 SEDFSDFNEVTSLIVSLLFKHHWAGVMKGMLALSSRQALILVMVLTVLWIIWKGKDRTHE 302 Query: 304 LTFLVWVVAGGELLDDMLRMVFHR--PGPVTAGVQLFNTFPSEETLISLTVCGFTAFLLL 361 ++ V GGEL ++L VFHR + +FPS++ ++ L + G+ +L++ Sbjct: 303 FLIMLLVTIGGELYQNVLHEVFHRMSETEIPTFSHFVFSFPSDQAMMILIIYGYFTYLVV 362 Query: 362 RYYSFRHIHTPIILGVILICFAVGISRIYFMVQYPSDVFAGYVFGGVWVSLNVMLLEILR 421 R+ +HT + + ++ + +S IYF ++ PS++ GYVFGGVW+ LN++LLEI R Sbjct: 363 RHAEMFWVHTFMDVLLLAVLLFAAVSHIYFGLEIPSNIAGGYVFGGVWLGLNILLLEIFR 422 Query: 422 KL 423 + Sbjct: 423 MI 424 >A6LYE8.1 A6LYE8_CLOB8 SNARE associated Golgi protein Length = 236 Score = 178 bits (451), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 80/171 (46%), Positives = 119/171 (69%) Query: 1 LDILTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGV 60 +D + L HYGY + + L LE++A PLPGE LM+Y G VY K+S++L I+ A +G Sbjct: 1 MDHVIELFNHYGYIAVLISLVLELIAFPLPGEALMTYCGYVVYMNKMSYLLSIIVATSGA 60 Query: 61 LLGVTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHI 120 ++G+T+SY++G L I YG+ IHL + R+ K++ W++KYG LL VAYFIPG+RHI Sbjct: 61 IIGITISYFIGRALEIKLIENYGSYIHLNKNRMDKISTWYQKYGGILLIVAYFIPGIRHI 120 Query: 121 TGYFCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKWEQYHSTVNRY 171 TGYF G+T++ ++++A+ AY GA+ W + FISLG+VLG W +Y+ Y Sbjct: 121 TGYFSGMTKVSYKKFAISAYLGALIWTTTFISLGRVLGANWNKYNEISKWY 171 >B1HZT2.1 B1HZT2_LYSSC Hypothetical ybfM protein Length = 198 Score = 152 bits (384), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 82/188 (43%), Positives = 120/188 (63%), Gaps = 5/188 (2%) Query: 7 LLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGVLLGVTL 66 L+EHYGY I + L ++ LPLP E+ ++Y+G SVY + L + +VSA G + G+TL Sbjct: 8 LIEHYGYFGIIVILIGGIVGLPLPDEVFLTYVGYSVYRESLGHIPALVSALIGAMGGITL 67 Query: 67 SYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHITGYFCG 126 SY++G R G P + +YG +IH+TE++I + F K G LL YFIPGVRH+T Y Sbjct: 68 SYYIGRRFGLPLLQKYGPKIHITEQKIEFTKKMFMKVGPFLLIAGYFIPGVRHLTAYIAA 127 Query: 127 VTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKWE---QYHSTVNRYLIIFTVVSILLT 183 + P+R++A FAY+GA W FI+LGKVLG KW Y S + Y+I+ V+ +L+ Sbjct: 128 INNYPYRKFAFFAYTGAFIWTFTFITLGKVLGEKWRFVGYYLSHYSMYVILLFVLVMLI- 186 Query: 184 AVIYLYRR 191 V YL+++ Sbjct: 187 -VYYLFKK 193 >C3EFP4.1 C3EFP4_BACTK Alkaline phosphatase, DedA Length = 197 Score = 151 bits (382), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 78/192 (40%), Positives = 120/192 (62%) Query: 4 LTHLLEHYGYGLIFLFLCLEMLALPLPGEMLMSYIGLSVYEQKLSWMLCIVSAGTGVLLG 63 + L+ HYGY I + L ++ LP+P E L++++G +V + +S +S G +LG Sbjct: 5 IGELIAHYGYFGIVIALAGGIVGLPIPDEFLLTFVGYNVSKGVMSGTAAFLSGMAGAMLG 64 Query: 64 VTLSYWVGYRLGRPFIARYGARIHLTEERIVKMNRWFEKYGDKLLFVAYFIPGVRHITGY 123 +TLSY +G +LG P + +YG +I + E I K + FEKYG LL + YFIPGVRH+T Y Sbjct: 65 ITLSYILGLKLGLPVLKKYGPKIRIKEHHIEKTHILFEKYGPFLLMIGYFIPGVRHLTAY 124 Query: 124 FCGVTRMPFRRYALFAYSGAIFWVSLFISLGKVLGPKWEQYHSTVNRYLIIFTVVSILLT 183 F GV+ + F R+ L+AY GA+ W+S+FI LG LG KW +++ Y I ++S ++T Sbjct: 125 FAGVSNLTFWRFCLYAYGGALIWISVFIGLGWKLGEKWRFVEYSLHHYGIWILLISAVVT 184 Query: 184 AVIYLYRRYKHQ 195 V++LY + K Sbjct: 185 CVVWLYIKKKKN 196 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.147 0.471 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,580,847,483 Number of extensions: 106906952 Number of successful extensions: 351271 Number of sequences better than 1.0e-05: 2132 Number of HSP's gapped: 351178 Number of HSP's successfully gapped: 2134 Length of query: 428 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 289 Effective length of database: 1,751,175,118 Effective search space: 506089609102 Effective search space used: 506089609102 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 133 (55.8 bits)