BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0513.0006.p (219 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q0RMY1.1 Q0RMY1_FRAAA Putative short chain dehydrogenase EC=1.1.1.- 195 3e-48 C1REJ5.1 C1REJ5_9ACTO Putative uncharacterized protein 172 2e-41 C1WW98.1 C1WW98_9ACTO Putative uncharacterized protein 167 9e-40 Q0RIR8.1 Q0RIR8_FRAAA Putative oxidoreductase 154 7e-36 Q5YNF1.1 Q5YNF1_NOCFA Putative short chain dehydrogenase 153 9e-36 >Q0RMY1.1 Q0RMY1_FRAAA Putative short chain dehydrogenase EC=1.1.1.- Length = 224 Score = 195 bits (495), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 102/225 (45%), Positives = 148/225 (65%), Gaps = 17/225 (7%) Query: 1 MKTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADIL 60 M TIAIVGAG GLG+SIA++FG+ GF VAL+SRN+ L+ L +L Q GI AA F AD+L Sbjct: 1 MPTIAIVGAGPGLGLSIARRFGREGFAVALLSRNQTTLDGLAAQLAQDGITAAGFAADVL 60 Query: 61 NKEQIEVAFAAIKEKYGFIDVLEYSPTP-----SINTVTPTLDVTEENALYQFQFNVLGA 115 ++ + A + + G IDVLEYSP P ++N + LDVT E+ + ++ V GA Sbjct: 61 DRPSLVDALTRAETQLGPIDVLEYSPAPAGRQNALNPIVEALDVTVESIQPEIEYYVYGA 120 Query: 116 LSSVRQVLPEMLDKQSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGV 175 +++V+ VLP M+++ +G LL TTG ++ P MGNVG+A +GLRN++ LN+ LADKGV Sbjct: 121 VTAVQHVLPGMIERGTGTLLVTTGASSALVFPRMGNVGLATAGLRNWVLALNAALADKGV 180 Query: 176 YAGHLSIGIWM-------QPNSGVQDKIADIWYDMYTKRDRVEEF 213 YA H+ + ++ QP D IA+I++++YTKRD+ E Sbjct: 181 YAAHVPLATFIGRGGPETQP-----DTIAEIYWELYTKRDQAERL 220 >C1REJ5.1 C1REJ5_9ACTO Putative uncharacterized protein Length = 236 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 92/227 (40%), Positives = 132/227 (58%), Gaps = 17/227 (7%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 K IA++GAG GLG SIA++F GFR+AL++R +E+L+ LV EL GIEA F AD+ Sbjct: 3 KVIAVLGAGQGLGTSIARRFAAEGFRIALVARRKERLDALVDELSAGGIEAVGFTADLSA 62 Query: 62 KEQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQ 121 E+I AI+E++G IDV+EY P P + TP ++ + +L L+ V+ Sbjct: 63 PEEIPTLMQAIRERFGQIDVIEYGPIPG-SHFTPAAELRPDALAQDIPLLLLTPLAIVQA 121 Query: 122 VLPEMLDKQSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGVYAGHLS 181 VL E + GA L TTG A+HP+P VG ++ RN++++LN ELA G+YAG LS Sbjct: 122 VLAEWKQRGDGAFLMTTGATAVHPMPYWSGVGPLMAAARNWLYSLNGELAPAGIYAGTLS 181 Query: 182 IGIWMQPNSGVQDK----------------IADIWYDMYTKRDRVEE 212 I ++ + Q +AD+++DMY KRDRVEE Sbjct: 182 IAAFITGSEAAQAAATDPKAASFPVVDPADLADLYWDMYAKRDRVEE 228 >C1WW98.1 C1WW98_9ACTO Putative uncharacterized protein Length = 235 Score = 167 bits (422), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 89/215 (41%), Positives = 132/215 (61%), Gaps = 3/215 (1%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 KT+AIVGAG GLG +A+ FG+ G+RVALI R L L +L GI+A F ADI + Sbjct: 7 KTLAIVGAGPGLGAGVARAFGRRGYRVALIGRTRATLISLAEKLAADGIQAEAFPADIHH 66 Query: 62 KEQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQ 121 +E + A +G I VLEY P+P+ +T L T E+A QF+ ++LGA+++V + Sbjct: 67 REALRSALREAAAAFGPITVLEYGPSPT-GEITTALGTTVESATQQFELHMLGAITAVGE 125 Query: 122 VLPEMLDKQSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGVYAGHLS 181 VLP ML + GA+L TTG ++ P P +GNVG A++ L+N+ L +E+ +GVY G L+ Sbjct: 126 VLPGMLARGDGAVLLTTGVSSTVPAPFLGNVGPAMAALKNWAGALATEVRARGVYVGTLT 185 Query: 182 IGIWMQPNS--GVQDKIADIWYDMYTKRDRVEEFI 214 I + P S G D I + +Y++ +RD E+ + Sbjct: 186 IATGVVPGSPDGDPDLIGERYYELARRRDHFEQTV 220 >Q0RIR8.1 Q0RIR8_FRAAA Putative oxidoreductase Length = 228 Score = 154 bits (388), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 84/203 (41%), Positives = 125/203 (61%), Gaps = 1/203 (0%) Query: 10 GAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILNKEQIEVAF 69 G +G+++A++FG+ GF ALI+R+ ++L V EL + G+ A+ AD N + A Sbjct: 2 GPRIGLAVARRFGREGFHPALIARDADRLTGHVAELAERGVTASAHPADAANPAALAGAL 61 Query: 70 AAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQVLPEMLDK 129 A ++ +G +DVL Y+ + VT LDVT ++A +VLGA+S+VR VLP MLD+ Sbjct: 62 AGVERVHGPVDVLVYNAVGAGRGVTAVLDVTVDSARDALDVSVLGAVSAVRAVLPGMLDR 121 Query: 130 QSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGVYAGHLSIGIWMQPN 189 +G LLFT+G +AIHP P +GNVG+A +GLRNY+ L L GV GH+ IG + P Sbjct: 122 GAGTLLFTSGISAIHPFPFLGNVGLAAAGLRNYVLALAEALERSGVQVGHVPIGAPVAPG 181 Query: 190 S-GVQDKIADIWYDMYTKRDRVE 211 S + +AD + ++ RDR E Sbjct: 182 SPAAPEAVADAHWRLHADRDRHE 204 >Q5YNF1.1 Q5YNF1_NOCFA Putative short chain dehydrogenase Length = 241 Score = 153 bits (387), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 131/230 (56%), Gaps = 15/230 (6%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 K IA++GAG GLG ++A++FG+ GFRVAL++R ++L+ LV L GIEAA F AD+ + Sbjct: 3 KVIAVLGAGTGLGSAVARRFGREGFRVALVARRRDRLDGLVDRLTAEGIEAAGFCADLSD 62 Query: 62 KEQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQ 121 + AA+++++G ID +EY P TP ++ +L + VR Sbjct: 63 PGAVPGLLAAVRQRFGRIDAVEYGPIGGAQNFTPATELDAAALAELVPLLLLTPVEVVRG 122 Query: 122 VLPEMLDKQSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGVYAGHLS 181 VLPE ++ GA L T G +A+ P+P + VG ++ RNY+++L+ ELAD+GVYAG L+ Sbjct: 123 VLPEWTERGDGAFLLTHGYSAVEPMPYLSGVGPIMAAARNYLYSLHGELADRGVYAGTLA 182 Query: 182 IGIWM---------QPNSGVQ------DKIADIWYDMYTKRDRVEEFITE 216 + ++ + ++G + D +A+ ++ MY RD E E Sbjct: 183 VAAYIAGSEIAEAARASTGAELPVADPDDLAEQYWSMYVARDGAERVHPE 232 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,290,075,096 Number of extensions: 52942833 Number of successful extensions: 187970 Number of sequences better than 1.0e-05: 4387 Number of HSP's gapped: 193065 Number of HSP's successfully gapped: 4387 Length of query: 219 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 89 Effective length of database: 1,835,964,253 Effective search space: 163400818517 Effective search space used: 163400818517 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)