BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0513.0002.p (533 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8R821.1 A8R821_9FIRM Putative uncharacterized protein 184 2e-44 B9YCI5.1 B9YCI5_9FIRM Putative uncharacterized protein 145 9e-33 A8MI75.1 A8MI75_ALKOO Peptidase U32 141 2e-31 A6TNQ1.1 A6TNQ1_ALKMQ Peptidase U32 133 4e-29 Q97GM5.1 Q97GM5_CLOAB Collagenase family protease 110 3e-22 >A8R821.1 A8R821_9FIRM Putative uncharacterized protein Length = 756 Score = 184 bits (466), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 156/550 (28%), Positives = 260/550 (47%), Gaps = 59/550 (10%) Query: 1 LNTIDYTHDLQE--IDSLKIEGRMKVPTYVANVVSKYRMALD-------HNITAEDKENL 51 LNT+ + +L + + S KIEGRMK P YVA + + YR A+D ++A +E + Sbjct: 225 LNTLSHIGELIDAGVYSFKIEGRMKRPEYVALMTACYRKAIDAYMAKEPFEVSAALREEM 284 Query: 52 KKTFNRTFTXXXXXXXXXFHEDRRNITNISRPNNFGYPIGTISKIEKDRYEIKLTRPINQ 111 +K FNR FT FHE ++ N RPN+ G IG I KD+ +I LT+ ++Q Sbjct: 285 QKIFNRGFTAGHI-----FHEMGSHLMNPLRPNHMGIEIGKIVSFTKDKVQILLTKQLSQ 339 Query: 112 NDTIRISHGNEDVNLTVAKLYNKDGGLINTADDVCYIKIKEKM--SMGDVVYKTKDFFYH 169 D IRI + ED T+ +LY +G L+ A+ +++ + G V KT D Sbjct: 340 GDGIRILNEKEDCGFTINRLY-VNGYLVKQAEAGTLVELDKTFYAQKGSQVLKTSDCKQL 398 Query: 170 KELEASLEK------EFKRFNLDIRVYARPDSKLVIDAEGLGFNYSYESEEMLGGAINNP 223 +L+ S +K + F + + +AR V+D + GF ES + + AI P Sbjct: 399 LDLQDSYKKNKRYVAAYAEFTMQLGAHARLS---VMDTD--GFYAVCESIDKVERAIKTP 453 Query: 224 TTKDQVIKQFSRLNDTIFELHQVDYE-ECNAFIPAKLLNAARRDIVLGLYDL-KLNSQQK 281 +K+++++Q + D+IF+L ++ + +P K +N RR+ + L + KL + Sbjct: 454 LSKERIVQQLQKSKDSIFQLEGIELTVDEQTTLPIKEINRMRREALEQLANQRKLRHPHR 513 Query: 282 RTKTVSAKEKISFAPVTPYLTASVTNKVQYDACVSCGIKEIY-FDNVVRRNQNDY----- 335 TK+++ ++ +P+ P + A V K QY+AC GI I+ ++ + + Q Y Sbjct: 514 LTKSIALPKQKEMSPLAP-IIAVVHTKEQYEACQEAGIAMIFMYEELHDKLQQGYVRIPR 572 Query: 336 --KEKEGQXXXXXXXXXXHYRDTNPFVTDYSLNVVNATSCYELYRLGAKRVTLSYELNKS 393 K+ Y PF+ D S N++N S ++ GA+ V S E + + Sbjct: 573 VMKQAYPDEFVMIQENGGSY-AKRPFLCDTSCNIINHHSAAFFFQRGARGVCFSLEASLA 631 Query: 394 QIEDLMIAY-----YKENDGYPALEMIVYGKTPLMFTKYCPMRKM------NQCRVCKTK 442 Q +++ AY K N YP VYG+ LM ++YC + + QC +C+ Sbjct: 632 QCCEIVKAYEASYQAKGNFIYP-----VYGREELMLSEYCAINAIEKDSDKKQCGLCRKA 686 Query: 443 SYQLKDEHGT-FPIISHEDCXXXXXXXXXXXXXDELPNIK--GIEALRLNFTVESKEQVV 499 Y L D G +P++ +DC ++ K GI + FT E+K Q V Sbjct: 687 EYALVDIKGQRYPLLCDKDCRMHILNAQPRNRIADIKQAKQVGITSFLCVFTKENKTQCV 746 Query: 500 QVIHMASGKL 509 V+ G++ Sbjct: 747 NVLQKLQGEV 756 >B9YCI5.1 B9YCI5_9FIRM Putative uncharacterized protein Length = 764 Score = 145 bits (366), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 133/505 (26%), Positives = 220/505 (43%), Gaps = 59/505 (11%) Query: 1 LNTIDYTHDL--QEIDSLKIEGRMKVPTYVANVVSKYRMALDHNIT-----AEDKEN--L 51 LNT+D +L S KIEGRMK P YV VV YR +D + +E+ E L Sbjct: 223 LNTLDQVEELIRSGATSFKIEGRMKRPEYVGAVVRAYREKIDAVLAGMRPASEEGEQIRL 282 Query: 52 KKTFNRTFTXXXXXXXXXFHEDRRNITNISRPNNFGYPIGTISKIEKDRYEIKLTRPINQ 111 KK FNR FT FH+ + N RPN+ G IGT+ R ++KLT P++Q Sbjct: 283 KKLFNRGFTTGYL-----FHQSGPALMNPLRPNHVGIRIGTVLSQRNGRVQVKLTAPLHQ 337 Query: 112 NDTIRISHGN-EDVNLTVAKLYNKDGGLI---NTADDVCYIKIKEKMSMGDVVYKTKDFF 167 D +RI N ED+ +Y D L+ ++ + ++EK+ + +T D Sbjct: 338 GDGLRILRENGEDIGFIANYIYKHD--LLCAQGQPGEIIELPVREKVRPEAALLQTTDSE 395 Query: 168 YHKELEASLEKEFKRFNLDIRVYARPDSKLVID-AEGLGFNYSYESEEMLGGAINNPTTK 226 KE++A + +R + + R +++D + +GF +E++L A P Sbjct: 396 LMKEIQAQSAQPARRTAVRFTIRGRAGEPMLLDVCDEMGFQAQARTEQVLQPAQKAPLDT 455 Query: 227 DQVIKQFSRLNDTIFELHQVDYE-ECNAFIPAKLLNAARRDIVLGL-------YDLKLNS 278 ++ +Q +LNDT F+L VD + E F+P + +N ARR + L Y + Sbjct: 456 GRLTQQLGKLNDTPFQLESVDNQLEGALFLPVQQINEARRQALDHLAQRRAVRYPERAQL 515 Query: 279 QQKRTKTVSAKEKISFAPVTPYLTASVTNKVQYDACVSCGIKEI------------YFDN 326 Q++ + ++ EK P L + + Q +A ++ I + Sbjct: 516 QRQAVQPLTIHEKQPL----PGLIVQIETREQAEALAELPVQLISSVPALIQAYPQIMPS 571 Query: 327 VVRRNQNDYK-EKEGQXXXXXXXXXXHYRDTNPFVTDYSLNVVNATS-CYELYRLGAKRV 384 +R ++ + E E R T + NV N+ + L + V Sbjct: 572 TLRVQEHPARYETE---WLQAEEIGTLIRTTTRLICTSGFNVTNSEALALLLSQTSVAGV 628 Query: 385 TLSYELNKSQIEDLMIAYYKENDGYPALEM-IVYGKTPLMFTKYCPMRKM------NQCR 437 S E+N Q + A +KE G+ A + VYG LM +K CP+ ++ C+ Sbjct: 629 EFSLEINPIQQSAIREA-FKEQYGFSAPTVQYVYGYRDLMVSKACPVNRLLKDGTKRNCQ 687 Query: 438 VCKTKSYQLKDEHG-TFPIISHEDC 461 +C ++Y LK++ G +P+ C Sbjct: 688 LCHQQAYALKNKKGELYPVFGDAAC 712 >A8MI75.1 A8MI75_ALKOO Peptidase U32 Length = 837 Score = 141 bits (356), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 162/618 (26%), Positives = 272/618 (44%), Gaps = 106/618 (17%) Query: 1 LNTIDYTHDLQE--IDSLKIEGRMKVPTYVANVVSKYRMALDHNITAED-------KENL 51 LNT++ L E + S KIEGRMK P YVA++V YR A+D ++ +E + Sbjct: 231 LNTLEEIGQLIEAGVTSFKIEGRMKKPQYVASIVRGYRKAIDLYLSDRKALKDDQLQEEI 290 Query: 52 KKTFNRTFTXXXXXXXXXFHEDRRNITNISRPNNFGYPIGTISKIEK--DRYEIKLTRPI 109 + FNR FT FH + + NI +PNN G +G I + + +R+ IKL I Sbjct: 291 AQMFNRKFT-----KGHLFHSPKAEVINIEKPNNRGLYLGRIEDVNRKENRFRIKLEVDI 345 Query: 110 NQNDTIRI-SHGNEDVNLTVAKLYNKDGGLINTA--DDVCYIKIKEKMSMGDVVYKTKDF 166 D + I + +D+ + +Y +D L+ A +V ++++ + GD VYKT + Sbjct: 346 AVGDGVEIWNESTDDMGGNIRNIY-RDHQLVKEAKKGEVVELEMRCSVQKGDQVYKTLNL 404 Query: 167 FYHKELEASL--EKEFKRFNLDIRVYARPDSKL-VIDAEGLGFNYSYESEEMLGGAINNP 223 LE + +KE+K+ L +V + K+ V + G +S+ ++ A+ Sbjct: 405 SLMDALEKTYAQDKEYKKIPLYGKVQVELNEKIKVCLWDNEGHAVEAKSDAVVEAALKVA 464 Query: 224 TTKDQVIKQFSRLNDTIFELHQVDY---EECNAFIPAKLLNAARRDIVLGLYDLKLNSQQ 280 T++++++Q S+L +T F+L +D E C+ +P +LN+ RR+IV L DL+ N Sbjct: 465 VTEEKLLEQLSKLGNTPFQLEHLDVSLGENCS--VPVSVLNSLRREIVDQLMDLRKNRHN 522 Query: 281 KRT-----------KTVSAKEKISFAPVTPY-LTASV-------------TNKVQY---- 311 + T +S E+ + P+ L+ V +V Y Sbjct: 523 RETIENQDFNEKLRSWISNSERKNKEPLASLRLSVKVDTLHQLKEVLKHDVGRVYYEDIA 582 Query: 312 ---DACVSC------GIKEIYF--DNVVRRNQNDYKEK-------EGQXXXXXXXXXXHY 353 DA C + EIY N++R ++ D E +G + Sbjct: 583 TFKDAIGMCREKNTAALTEIYLRTPNILRNSEYDRLENLVEGLSFDGILAGDVGLIRWNK 642 Query: 354 RDTN-PFVTDYSLNVVNATSCYELYRLGAKRVTLSYELNKSQIEDLMIAYYKENDGYPAL 412 N P DY+LNV N S + L +TLS EL+ + + L D AL Sbjct: 643 EHANLPVFGDYTLNVFNRPSIHAFEGLDFNGITLSPELDFNSLYQL--------DFNNAL 694 Query: 413 --EMIVYGKTPLMFTKYCPMRKMNQ----CRVCKTKSYQ----LKD-EHGTFPIISHEDC 461 E ++YGK +M T+YCP + NQ C C+ Y L D ++ +FPI + Sbjct: 695 EKEAMIYGKVVVMTTEYCPFIQENQCDHNCNQCRYPKYHYNFGLNDAKNVSFPIAKNYWG 754 Query: 462 XXXXXXXXXXXXXDELPNIKG--IEALRLNFTVESKEQVVQVIHMASGKLNGSMNNAVFN 519 D L K ++ LRL FT E+ E++ +++ +A ++ ++ Sbjct: 755 RTIILNSKPLFMIDRLHEFKDVPVQWLRLEFTDETIEEIKELLELAEKNIHRALTGEKSK 814 Query: 520 QETD---------TRGHF 528 ++ + TRGH+ Sbjct: 815 EDIEVERIFKDGFTRGHY 832 >A6TNQ1.1 A6TNQ1_ALKMQ Peptidase U32 Length = 807 Score = 133 bits (335), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 156/611 (25%), Positives = 251/611 (41%), Gaps = 110/611 (18%) Query: 1 LNTIDYTHDLQE--IDSLKIEGRMKVPTYVANVVSKYRMALD----HNITAEDKE---NL 51 LNT D+ H L + + S KIEGRMK P YVA++V YR A+D HN +D+E + Sbjct: 227 LNTFDHIHQLIDSGVTSFKIEGRMKKPQYVASIVGSYRQAIDHYLEHNQAFKDEELQWEM 286 Query: 52 KKTFNRTFTXXXXXXXXXFHEDRRNITNISRPNNFGYPIGTIS--KIEKDRYEIKLTRPI 109 ++ FNR FT F+ + + NI +PNN G +G + + + +IKL R I Sbjct: 287 EQMFNRKFT-----KGYLFNSQKTEVINIQKPNNRGVSLGEVMDYHTKTKKMKIKLLRDI 341 Query: 110 NQNDTIRI-SHGNEDVNLTVAKLYNKDGGLINTADDVCYIKIKEKMSMGDVVYKTKDFFY 168 D I + E++ +V K+ G ++ I+++ + GD VYKT D Sbjct: 342 EPGDGIEVWGQKKEEIGGSV-KIQGSKGQVVE-------IEMRGNIQPGDEVYKTLDISL 393 Query: 169 HKELEASLEKEFKRFNLDIRV-----YARPDSKLVIDAEGLGFNYSY-ESEEMLGGAINN 222 +KELE + ++ + ++IR P + D G N ++ +S+ ++ A Sbjct: 394 NKELEKTYHRQVENRKINIRAEIKVELGNPCELSLWDDRG---NTAFVKSDFIVEAAQKV 450 Query: 223 PTTKDQVIKQFSRLNDTIFELHQVDYE-ECNAFIPAKLLNAARRDIVLGLYDLKLNSQQK 281 K++++K S+L DT + L V + N +P ++N RR + +N+Q Sbjct: 451 AVDKEKIVKNVSKLGDTPYHLENVQVTLDPNVSVPLSVINDIRRKAI-----EIINAQ-- 503 Query: 282 RTKTVSAKEKISFAPVTPYLTASVTNKVQYDA-----CVSCG----IKEIYFDNVVRRNQ 332 + V K + P P L + +K + + V C ++E+ +V R Sbjct: 504 --RNVGYKGRPDVIPHLPMLNDIIEDKYSHASRRKGLVVKCDSIMHLREVVTKDVHRVYY 561 Query: 333 NDYKEKEGQXXXXXXXXXXHYRDTNPFVTD------------------------------ 362 D+K + Y + + D Sbjct: 562 GDFKTLQVAIDLCHGKKIEIYFKSPSIIKDQEMTHLVEGLSKLKIDGVLAGDLGMLNVSI 621 Query: 363 ----------YSLNVVNATSCYELYRLGAKRVTLSYELNKSQIEDLMIAYYKENDGYPAL 412 YSLN+ N+ + + +GAK +T S EL +++ L K G Sbjct: 622 QNLKIPTIADYSLNIFNSYTAQLMADMGAKGMTPSLELTLREMKGL-----KSIKGLDT- 675 Query: 413 EMIVYGKTPLMFTKYCPMRKM----NQCRVCKTKSYQ----LKDEHG-TFPIISHEDCXX 463 E + YGK P+M +YCP+ ++ QC CK Y LKD FP Sbjct: 676 EAVSYGKLPIMTMEYCPLVQVEKCDQQCAPCKYYQYDYRWGLKDHKDIIFPFTKDYWGRT 735 Query: 464 XXXXXXXXXXXDELPNIKG--IEALRLNFTVESKEQVVQVIHMASGKLNGSMNNAVFNQE 521 D+L + K ++ LRL FT E+KE V I L G Q Sbjct: 736 ILFNGQTLYLADKLSDFKDLKVKWLRLEFTDETKEDVTSTITQYEKGLMGKEEPLKKKQG 795 Query: 522 TDTRGHFNKEI 532 T TRGH+ + + Sbjct: 796 TFTRGHYYRGV 806 >Q97GM5.1 Q97GM5_CLOAB Collagenase family protease Length = 787 Score = 110 bits (276), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 149/574 (25%), Positives = 235/574 (40%), Gaps = 87/574 (15%) Query: 15 SLKIEGRMKVPTYVANVVSKYRMALDHNITA---EDKENLKKTFNRTFTXXXXXXXXXFH 71 SLKIEGRMK P Y VVS YR A+D+ + E+K L K FNR Sbjct: 237 SLKIEGRMKKPEYAGGVVSSYRKAIDNKLNGDYKEEKAKLLKLFNRE----GFSRAYLLG 292 Query: 72 EDRRNITNISRPNNFGYPIGTISKIEKDRYEIKLTRPINQNDTIRISHGNEDVNLTVAKL 131 +++ P N G IG K+ D I L +N D +R DV TV Sbjct: 293 NTGKDMMAYKFPKNTGVFIG---KVISDG-SIVLKESLNLKDGVR-----NDVKGTVVSK 343 Query: 132 YNKDGGLINTADDVCYIKIKEKM-SMGDVVYKTKDFFYHKELEASLEKEFKR-FNLDIRV 189 DG + A +KI + GD +YKT D +E+E S +K+FKR LD+ + Sbjct: 344 ILLDGVEVKEAKPGEKVKIFPRFYKSGDYIYKTLDLKLTREIEESYKKDFKRKVYLDLEI 403 Query: 190 YARPDSKLVIDAE-----GLGFNYSYES------EEMLGGAINNPTTKDQVIKQFSRLND 238 + I A LG N + +M+ A +P +++V + + Sbjct: 404 EFKIGEPFKITASFSRVNDLGANEKNRAISIQVLGDMVCKAEKSPVKREKVEEHLRKTGG 463 Query: 239 TIFELHQVDYEEC--NAFIPAKLLNAARRDIVLGLYDLKLNSQQKRTKTVSAKEKISFAP 296 T FE+ +++ C +IP LN+ RR+ VL + + KR + E I + Sbjct: 464 TPFEVRNIEFT-CFDEGYIPISALNSIRRE-VLEKFQNSIQKSYKREANLKF-EGIKISK 520 Query: 297 VT----PYLTASVTNKVQYDACVSCGIKEIYFD---------------------NVVRRN 331 + P + V+NK QY A G K I D NV++ Sbjct: 521 RSEVKIPEIMVLVSNKEQYLAVKEEGFKNIIVDLFKRKCDLKLKELKDVYIKVPNVIKEE 580 Query: 332 QNDYKEKEGQ-------XXXXXXXXXXHYRDTNPFVTDYSLNVVNATSCYELYRLGAKRV 384 ND K + Y+ + DY N+ N+ + E Y+ Sbjct: 581 FNDICNKIDENIENIKGIVTVNAGIINKYKGLVNIIGDYKGNIFNSEA-LEFYKEDLDGF 639 Query: 385 TLSYELNKSQIEDLMIAYYKENDGYPALEMIVYGKTPLMFTKYCPM--------RKMNQC 436 +S EL+K++I+ L + +M++YGK M ++YCP+ K N Sbjct: 640 CISTELSKNEIKALNKCKCMDT------QMLIYGKIEAMVSEYCPIGSVFGGKSSKSNCQ 693 Query: 437 RVCKTKSYQLKDEHG-TFPIISHEDCXXXXXXXXXXXXXDELPNIK--GIEALRLNFTVE 493 C + LKD+ G +F +++ + C L IK G+ + R++F E Sbjct: 694 GTCMKSDFVLKDKMGVSFDVVTDKYCRSHIYNPLPLNLIGNLDEIKNLGVNSYRIDFIDE 753 Query: 494 SKEQVVQVI-HMASGKLNGSMNNAVFNQETDTRG 526 ++E V +V+ M +GK++G + F + + RG Sbjct: 754 NRESVKRVLSWMKNGKIDGEFSG--FTRGSYKRG 785 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,855,448,632 Number of extensions: 112425711 Number of successful extensions: 249444 Number of sequences better than 1.0e-05: 156 Number of HSP's gapped: 249457 Number of HSP's successfully gapped: 181 Length of query: 533 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 392 Effective length of database: 1,732,333,088 Effective search space: 679074570496 Effective search space used: 679074570496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)