BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0512.0005.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH72.1 B3KH72_9BACI Lipase class 3 344 4e-93 B1DH16.1 B1DH16_9BACL Lipase class 3 232 3e-59 C0ZGR4.1 C0ZGR4_BREBN Putative uncharacterized protein 189 2e-46 C0ZGR3.1 C0ZGR3_BREBN Putative uncharacterized protein 180 1e-43 C2YY94.1 C2YY94_BACCE Lipase 161 6e-38 >B3KH72.1 B3KH72_9BACI Lipase class 3 Length = 270 Score = 344 bits (883), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 165/266 (62%), Positives = 205/266 (77%) Query: 1 MHVSNNQAFEERAIFLAAVCGQTYAQFNHADGSFTIPAGYSVTHTIQAKSLGQVWERFGF 60 M++ + E+ AIFLAA+CGQTY QF DGSF +P +S+ TI+A SL +V ERFGF Sbjct: 1 MYMGASTEQEQWAIFLAAICGQTYTQFTSTDGSFVLPLNFSLFDTIEANSLIRVSERFGF 60 Query: 61 IIESPEEIIIAFRGSSSTANWISNANATQKKFKYIQEDCLTHRGFTNIYTSARSGIHSAL 120 I+ESPEEIIIAFRG+ S+ +WI++A A+QK FKYI++ LTHRGFT+IY SAR I SAL Sbjct: 61 ILESPEEIIIAFRGTMSSTDWITDAIASQKNFKYIKDPALTHRGFTSIYASARGQIMSAL 120 Query: 121 SRLSSGKRLYITGHSLGGALATLCAPDVAANTSFSTPHLYTYGSPRVGDPAFAKASTRYL 180 +RL K L+ITGHSLGGALATLCA DVAANT +PH++TYGSPRVGDP FAKA +Y+ Sbjct: 121 ARLPVDKTLFITGHSLGGALATLCAVDVAANTDHQSPHVFTYGSPRVGDPDFAKAFAKYV 180 Query: 181 PNSHRIANLFDSAAYVPPSIFKLPKRDKRYYYSHVREFCPLSFQKGSVSLNHALSSYFEE 240 +S RIANLFD + PPSI+KLPKR+K+YYYSHV PL+FQ GSVS NH +SSY+ E Sbjct: 181 RSSFRIANLFDVVTHAPPSIYKLPKREKKYYYSHVHTHWPLTFQNGSVSGNHVISSYYAE 240 Query: 241 LSMLRPEFTRQLCTSNPDFCPVLEVP 266 L+ L+P F L ++NP FCPV E+P Sbjct: 241 LAKLQPPFAELLNSANPGFCPVTELP 266 >B1DH16.1 B1DH16_9BACL Lipase class 3 Length = 283 Score = 232 bits (591), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 112/263 (42%), Positives = 161/263 (61%), Gaps = 1/263 (0%) Query: 1 MHVSNNQAFEER-AIFLAAVCGQTYAQFNHADGSFTIPAGYSVTHTIQAKSLGQVWERFG 59 M Q F+ R A+FLA + GQTY+QF + DG F +P GY + AK+ ERFG Sbjct: 20 MRQGQPQQFDLRIALFLAGIAGQTYSQFENKDGLFLVPRGYELVGDFSAKAYDDTEERFG 79 Query: 60 FIIESPEEIIIAFRGSSSTANWISNANATQKKFKYIQEDCLTHRGFTNIYTSARSGIHSA 119 F+++S ++AFRGS S +W+S+ A Q ++ ++ TH+GFT+IYTS RS + Sbjct: 80 FVLQSDRSSVLAFRGSGSAVDWVSDFIAQQTTYRPVKNAGQTHKGFTDIYTSTRSQVLDL 139 Query: 120 LSRLSSGKRLYITGHSLGGALATLCAPDVAANTSFSTPHLYTYGSPRVGDPAFAKASTRY 179 +++L K L+ITGHSLGGALATL A D+A NT F+ P +YT+G+PRVGD F K Sbjct: 140 IAQLPVEKPLFITGHSLGGALATLAALDIAVNTPFTAPIIYTFGAPRVGDTRFVKLYNNT 199 Query: 180 LPNSHRIANLFDSAAYVPPSIFKLPKRDKRYYYSHVREFCPLSFQKGSVSLNHALSSYFE 239 + R+ N +D ++PP +++ P K Y+Y HV+ F+ GSVS NH LSSYF Sbjct: 200 VETHWRLQNEYDIVPHLPPLVYQSPDTKKTYFYMHVKGEVKRGFRMGSVSGNHILSSYFA 259 Query: 240 ELSMLRPEFTRQLCTSNPDFCPV 262 +L P+F +C+ P +CP+ Sbjct: 260 DLCGEEPQFAEAICSEPPGWCPL 282 >C0ZGR4.1 C0ZGR4_BREBN Putative uncharacterized protein Length = 272 Score = 189 bits (481), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 106/254 (41%), Positives = 144/254 (56%), Gaps = 5/254 (1%) Query: 11 ERAIFLAAVCGQTYAQFNHADGSFTIPAGYSVTHTIQAKSLGQVWE--RFGFIIESPEEI 68 E AI LAA+ Q Y F S +P GY I+A + + E FGF+ ESP+ I Sbjct: 9 EDAILLAAMIHQAYQLFE--TDSLVLPQGYRQRSAIRALAGVETPEPEVFGFVAESPDSI 66 Query: 69 IIAFRGSSSTANWISNANATQKKFKYIQEDCLTHRGFTNIYTSARSGIHSALSRLSSGKR 128 ++A RG+ + + S+ + Q + ++++ THRGFT IY SAR + LS+LS KR Sbjct: 67 VVALRGTRTFNDNESDQDLYQVPYHFVRKAGKTHRGFTCIYQSARDELIRELSKLSRSKR 126 Query: 129 LYITGHSLGGALATLCAPDVAANTSFSTPHLYTYGSPRVGDPAFAKASTRYLPNSHRIAN 188 L++ GHSLGG LATL D+A NT F+ P +YTYGSPRVGDP FA + NS RI N Sbjct: 127 LFVAGHSLGGGLATLAGLDIAVNTKFTRPFVYTYGSPRVGDPVFASRFNETVKNSVRIVN 186 Query: 189 LFDSAAYVPPSIFKLPKRDKRYYYSHVREFCPLSFQKGSVSL-NHALSSYFEELSMLRPE 247 + D +P ++ P K YY HV L FQ S+++ NH + YF+ L+ P Sbjct: 187 VHDIIPTLPSKVYPPPFTKKGLYYQHVDRKHLLDFQLNSLAVRNHEIVCYFKFLAQEDPG 246 Query: 248 FTRQLCTSNPDFCP 261 FT +LC N FCP Sbjct: 247 FTERLCVENEGFCP 260 >C0ZGR3.1 C0ZGR3_BREBN Putative uncharacterized protein Length = 276 Score = 180 bits (456), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 98/261 (37%), Positives = 147/261 (56%), Gaps = 5/261 (1%) Query: 4 SNNQAFEERAIFLAAVCGQTYAQFNHADGSFTIPAGYSVTHTIQAKSLGQ--VWERFGFI 61 +N + AIFLAA+ QTY F G +P G+ + +TI+A + + +GF+ Sbjct: 6 EDNMFTSQAAIFLAAMSYQTYLLF--FKGELILPKGFQLRYTIRASADVENPTEHMYGFV 63 Query: 62 IESPEEIIIAFRGSSS-TANWISNANATQKKFKYIQEDCLTHRGFTNIYTSARSGIHSAL 120 ES ++IIIAFRG ++ A+ ++ + Q + ++ + T RGFT +Y S R + + Sbjct: 64 AESKDQIIIAFRGYAAYPADLLAAYDILQITYPFVTDAGKTSRGFTCLYQSTRDRLLRKI 123 Query: 121 SRLSSGKRLYITGHSLGGALATLCAPDVAANTSFSTPHLYTYGSPRVGDPAFAKASTRYL 180 ++ S K+L ITGH+ GGALA L A D+A NT F P +YTYGSPR+GDP FA + + Sbjct: 124 NQFSESKKLIITGHNYGGALAVLAALDIAVNTPFRHPIVYTYGSPRIGDPHFASRFNKVV 183 Query: 181 PNSHRIANLFDSAAYVPPSIFKLPKRDKRYYYSHVREFCPLSFQKGSVSLNHALSSYFEE 240 NS RI N+ D P + P + YY HV+ P+SFQ + N +++ YF Sbjct: 184 LNSLRIVNVHDPFPTFPDQKYPPPFTQEGIYYQHVKNKFPISFQLNNTPRNDSIACYFHS 243 Query: 241 LSMLRPEFTRQLCTSNPDFCP 261 L+ P++ + LC NP FCP Sbjct: 244 LTQFDPDYAQTLCVENPGFCP 264 >C2YY94.1 C2YY94_BACCE Lipase Length = 240 Score = 161 bits (407), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 90/228 (39%), Positives = 125/228 (54%), Gaps = 4/228 (1%) Query: 10 EERAIFLAAVCGQTYAQFNHADGSFTIPAGYSVTHTIQAKSLGQVWERFGFIIESPEEII 69 ++ AI LA+ C TY Q+ +G F IP G+ Q K++ Q E FGFI+ES + II Sbjct: 9 KDTAILLASCCELTYEQYKQ-NGIFEIPDGFQYVQGFQGKTI-QTTEWFGFILESEDTII 66 Query: 70 IAFRGSSSTANWISNANATQKKFKYIQEDCLTHRGFTNIYTSARSGIHSALSRLSSGKRL 129 +AFRG+ + +WI ++ QK + Y H GF +IY S R I L L + K+L Sbjct: 67 VAFRGTQTDTDWIIDSLVNQKPYPYALNSGNVHNGFLSIYESCRDSIMDMLVSLPAHKKL 126 Query: 130 YITGHSLGGALATLCAPDVAANTSFSTPHLYTYGSPRVGDPAFAKASTRYLPNSHRIANL 189 TGHSLGGALATL D NT+F+ LYT+ SP+VGD AF + +S R NL Sbjct: 127 LATGHSLGGALATLHILDARINTAFAQYGLYTFASPKVGDIAFRNYYKLQVASSFRFVNL 186 Query: 190 FDSAAYVPPSIFKLPKRDKRYYYSHVREFCPLSFQKGSVSLNHALSSY 237 FD +PP RD + Y+HV + S++ NHA+++Y Sbjct: 187 FDVVPLLPPRNINFNDRD--WEYAHVHHNMTFTKNTKSITNNHAITTY 232 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.132 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,627,991,957 Number of extensions: 63816201 Number of successful extensions: 150617 Number of sequences better than 1.0e-05: 406 Number of HSP's gapped: 150469 Number of HSP's successfully gapped: 407 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)