BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0510.0011.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KB09.1 Q9KB09_BACHD BH2127 protein 144 8e-33 A6CMG4.1 A6CMG4_9BACI Putative uncharacterized protein 125 4e-27 B8FUN7.1 B8FUN7_DESHD Sortase family protein 120 7e-26 A0Q262.1 A0Q262_CLONN Sortase family protein 109 1e-22 Q97MJ2.1 Q97MJ2_CLOAB Sortase (Surface protein transpeptidase), ... 95 5e-18 >Q9KB09.1 Q9KB09_BACHD BH2127 protein Length = 194 Score = 144 bits (362), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 81/213 (38%), Positives = 118/213 (55%), Gaps = 31/213 (14%) Query: 10 LIILAGILLMLYPK-ANEWYNDWXXXXXXXXXXXXYSEISPSPSPQSDLHKQYAEVTQLL 68 L I G+L M +P+ E+Y+ + ++ L Y ++ + L Sbjct: 8 LFISFGLLFMFFPRLQTEYYSFY----------------------ETRLIDSYEKINREL 45 Query: 69 AEESSEGDLAKATEAPAPEIKLGGKITGIIQIDKINLKLPVLEGXXXXXXXXXXXXXXET 128 S+E ++E P G+++I+KI+LKLPVL+G E+ Sbjct: 46 EAFSNEEPHLLSSEEP--------HAIGVLEIEKISLKLPVLQGVDQETLKVGAGHMIES 97 Query: 129 AAIGQVGNAAIAAHRSRTAGRLFNRLNEVEVGDTISVQTKEQDYQYVVYDISIVEPTDVS 188 + IG+ GNAAIAAHRSRT GR FNRL+EVEVGD I+V T Y+Y VY I++VEPT++ Sbjct: 98 SPIGEKGNAAIAAHRSRTYGRQFNRLDEVEVGDVITVTTNNHMYRYTVYSITVVEPTNID 157 Query: 189 VLNGHNDDKILTLITCDPLVNPTHRLIIHARLS 221 +L +LTLITCDP+ +PTHRLI+ A ++ Sbjct: 158 ILQHDGTAPVLTLITCDPVKDPTHRLIVQAEMT 190 >A6CMG4.1 A6CMG4_9BACI Putative uncharacterized protein Length = 241 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 88/245 (35%), Positives = 126/245 (51%), Gaps = 34/245 (13%) Query: 2 IFMRKF-----SYLIILAGILLMLYPKANEWYND---------WX-------------XX 34 +FMR ++L+I G+ +MLYP+ ++Y+ W Sbjct: 1 MFMRWMMKKVIAFLLIFIGLGVMLYPEVTDYYSAHKQEELIDIWENQEFAPGEDIGIFEQ 60 Query: 35 XXXXXXXXXYSEISPSPSPQSDLHKQYAEVTQLLAEESSEGDLAKATEAPAPEIKLGGKI 94 Y ++ S P S+ Q AE+ E S EG EA E L + Sbjct: 61 TNEQTAQTSYVQLD-SIFPSSEGTVQAAEI-----ESSQEGHNEPEAEAAEREQPLSESM 114 Query: 95 TGIIQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRL 154 GII IDKI++KLP+L G +A GQ GN+AIAAHRSR G++FNRL Sbjct: 115 MGIITIDKIDVKLPILYGASQYNLKYGAGYLSGSALPGQNGNSAIAAHRSREYGKMFNRL 174 Query: 155 NEVEVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHRL 214 +EV+ GD I+V+T+E ++Y V IV+P+D+SVL ++ + LTLITCDP+ NP +RL Sbjct: 175 DEVQEGDLITVKTREGTHEYKVTSTKIVKPSDLSVLQ-NSSEATLTLITCDPIDNPINRL 233 Query: 215 IIHAR 219 I+ A Sbjct: 234 IVKAE 238 >B8FUN7.1 B8FUN7_DESHD Sortase family protein Length = 222 Score = 120 bits (302), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 82/228 (35%), Positives = 111/228 (48%), Gaps = 29/228 (12%) Query: 8 SYLIILAGILLMLYPKANEWYNDWXXXX-----XXXXXXXXYSEISPSPSPQSDLHKQYA 62 S + I+AG+ L+ YPKA E ++ + E + P+P D Sbjct: 7 SIIFIVAGLTLLAYPKAAEMHSTYLQKKLVSEWQAALAAVDQGERAADPAPDQD------ 60 Query: 63 EVTQLLAEESSE-----GDLAKATEAPAPEIK-----LGGKITGIIQIDKINLKLPVLEG 112 AEESS GDL + E PE K + + G+++I+KI L+LP+L+G Sbjct: 61 ------AEESSPAKESPGDLPE--EFITPEEKDRQDYIHDTMEGMLKIEKIGLELPILKG 112 Query: 113 XXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLNEVEVGDTISVQTKEQDY 172 T +G+ GN AIA HRS T GR FNRL EV GD + V + Y Sbjct: 113 VTEANLDLSVAHMENTGNMGEPGNYAIAGHRSHTYGRQFNRLEEVNPGDRLEVTDGKTTY 172 Query: 173 QYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHRLIIHARL 220 Y V V+PT+ VLN + D +TLITC P++NPTHRLII L Sbjct: 173 TYTVTQKLYVDPTETYVLNSNKDISEITLITCHPMINPTHRLIIKGVL 220 >A0Q262.1 A0Q262_CLONN Sortase family protein Length = 197 Score = 109 bits (273), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 55/125 (44%), Positives = 72/125 (57%) Query: 94 ITGIIQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNR 153 + GI++IDKI+LKLP+L G GQVGN +A HRS+T GR FNR Sbjct: 69 VDGILKIDKISLKLPILHGCNQKNLKVSLSSLDNNVNPGQVGNYVVAGHRSKTYGRNFNR 128 Query: 154 LNEVEVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHR 213 LNEV D I V T Y+YV+ +V P + +LN + D+ +TLITC P+ PTHR Sbjct: 129 LNEVNKDDIIEVDTLTNKYKYVIQKKIVVRPEETWILNSNGKDREITLITCYPINKPTHR 188 Query: 214 LIIHA 218 LI+ Sbjct: 189 LILKG 193 >Q97MJ2.1 Q97MJ2_CLOAB Sortase (Surface protein transpeptidase), YHCS B.subtilis ortholog Length = 194 Score = 94.7 bits (234), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 51/124 (41%), Positives = 70/124 (56%), Gaps = 1/124 (0%) Query: 96 GIIQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLN 155 GI++I KI+LK+ + EG T+ GQ GN +A HRS T G FNRL Sbjct: 70 GILKIPKIDLKVAIGEGTDLKTLKYAVGHFRNTSMPGQNGNFCLAGHRSYTFGEYFNRLG 129 Query: 156 EVEVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHRLI 215 E+ GD I V+T ++Y VY +V P++V VL+ D +TL+TC P+ THRLI Sbjct: 130 EIGSGDEIDVETVNGTFKYKVYSTKVVLPSEVHVLD-QTKDPTMTLVTCTPIRIATHRLI 188 Query: 216 IHAR 219 I A+ Sbjct: 189 IKAK 192 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,088,945,579 Number of extensions: 37189102 Number of successful extensions: 93161 Number of sequences better than 1.0e-05: 528 Number of HSP's gapped: 93006 Number of HSP's successfully gapped: 528 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)