BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0510.0002.p (529 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5B8.1 B3K5B8_9BACI GMP synthase, large subunit 888 0.0 B1D8V4.1 B1D8V4_9BACL GMP synthase, large subunit 855 0.0 C0ZK57.1 C0ZK57_BREBN GMP synthase EC=6.3.5.2 798 0.0 Q9KF78.1 GUAA_BACHD Putative GMP synthase [glutamine-hydrolyzing... 766 0.0 A7GKG1.1 GUAA_BACCN GMP synthase [glutamine-hydrolyzing] EC=6.3.5.2 763 0.0 >B3K5B8.1 B3K5B8_9BACI GMP synthase, large subunit Length = 512 Score = 888 bits (2295), Expect = 0.0, Method: Compositional matrix adjust. Identities = 424/512 (82%), Positives = 461/512 (90%) Query: 18 MNKPSEIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPIEKIKALSPKGIVFSGGPSS 77 MNKP+EIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTP+EKIK LSP+GIVFSGGPSS Sbjct: 1 MNKPNEIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPVEKIKDLSPRGIVFSGGPSS 60 Query: 78 VYAEDAPHVDPAIYDLGLPIFGICYGMQLMAHQQGGKVERADKREYGKANVDFELDAVLA 137 VY+E+APHVDPAIYDLGLPIFGICYGMQLMA Q GKVERA KREYGKA ++F ++ L Sbjct: 61 VYSENAPHVDPAIYDLGLPIFGICYGMQLMAQQLDGKVERASKREYGKAELEFAPNSTLV 120 Query: 138 AGLESGQTVWMSHGDHVVELPAGFKLDAGTESAPIAAMSHADRKFFAVQFHPEVRHSVNG 197 G+E QTVWMSHGDHVV LP+GFKLDAGTESAPIAAMS+ D+K +AVQFHPEVRHSV+G Sbjct: 121 KGIEQKQTVWMSHGDHVVSLPSGFKLDAGTESAPIAAMSNEDKKLYAVQFHPEVRHSVHG 180 Query: 198 NEMISNFLYEVCGCEGKWTMESFIEDAVKDIQAKVGDKKVLCALSGGVDSSVVAMLIHRA 257 NEMI NFLYEVCGCEG W+ME+FIED V++I+ VG+KKVLCALSGGVDSSVVAMLIH+A Sbjct: 181 NEMIKNFLYEVCGCEGNWSMETFIEDQVREIREVVGEKKVLCALSGGVDSSVVAMLIHKA 240 Query: 258 IGDQLTCMFIDHGLLRKGEAESVMETFVGKFDIHVVKIDARERFLGKLAGVSDPEQKRKI 317 IGDQLTCMFIDHGLLRKGEAESVMETFVGKFD+ VVKIDA+ERF+ KL GV DPE+KRKI Sbjct: 241 IGDQLTCMFIDHGLLRKGEAESVMETFVGKFDMKVVKIDAQERFMSKLKGVDDPEKKRKI 300 Query: 318 IGNEFIYCFDEESAKLGEFSFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFSLI 377 IGNEFIY FDEES+K +F+FLAQGTLYTDIVESGT TA TIKSHHNVGGLPEDMKF LI Sbjct: 301 IGNEFIYVFDEESSKFDDFAFLAQGTLYTDIVESGTDTAHTIKSHHNVGGLPEDMKFELI 360 Query: 378 EPLNTLFKDEVRKLGEELGMPHAIVWRQPFPGPGLAIRVLGEVTEEKLEIVRNSDFILRE 437 EPL LFKDEVRK+GEELG+P IV RQPFPGPGLAIRVLGEVTE+KL+IVR+SD+ILRE Sbjct: 361 EPLKALFKDEVRKVGEELGLPDEIVHRQPFPGPGLAIRVLGEVTEDKLQIVRDSDYILRE 420 Query: 438 EIAKAGLDREIWQYFTALPNMKSVGVMGDERTYSYTVGIRAVTSIDGMTADWARIPWEVL 497 EIAKAGLDREIWQYFTALPNMKSVGVMGD RTYSYTVGIRAVTSIDGMTADWARIPW+VL Sbjct: 421 EIAKAGLDREIWQYFTALPNMKSVGVMGDARTYSYTVGIRAVTSIDGMTADWARIPWDVL 480 Query: 498 EKISXXXXXXXXXXXXXXYDITSKPPATIEWE 529 EKIS YD+TSKPPATIEWE Sbjct: 481 EKISVRIVNEVENVNRIVYDVTSKPPATIEWE 512 >B1D8V4.1 B1D8V4_9BACL GMP synthase, large subunit Length = 511 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/512 (81%), Positives = 449/512 (87%), Gaps = 1/512 (0%) Query: 18 MNKPSEIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPIEKIKALSPKGIVFSGGPSS 77 MNK +EII+VLDFGGQYNQLIARRIRDLGVYSELLP+NT +E+I+ L PKGIVFSGGP+S Sbjct: 1 MNK-NEIIIVLDFGGQYNQLIARRIRDLGVYSELLPFNTSVERIRELQPKGIVFSGGPAS 59 Query: 78 VYAEDAPHVDPAIYDLGLPIFGICYGMQLMAHQQGGKVERADKREYGKANVDFELDAVLA 137 VY E +P VDPAIYDLG+PIFGICYGMQ+M+HQ GKVERA KREYGKA VDF + LA Sbjct: 60 VYEEKSPLVDPAIYDLGVPIFGICYGMQMMSHQLEGKVERAGKREYGKAEVDFAEGSHLA 119 Query: 138 AGLESGQTVWMSHGDHVVELPAGFKLDAGTESAPIAAMSHADRKFFAVQFHPEVRHSVNG 197 GL++ QTVWMSH D VVE PAGF++DA TE APIAAMS+ DRKFFAVQFHPEVRHSV G Sbjct: 120 QGLDARQTVWMSHSDLVVEPPAGFRVDASTEHAPIAAMSNPDRKFFAVQFHPEVRHSVYG 179 Query: 198 NEMISNFLYEVCGCEGKWTMESFIEDAVKDIQAKVGDKKVLCALSGGVDSSVVAMLIHRA 257 NEMI NFLY +CGCEG WTMES+IED + DI+ +VGD+KVLC LSGGVDSSVVAMLIH+A Sbjct: 180 NEMIRNFLYNICGCEGNWTMESWIEDTITDIRNQVGDRKVLCGLSGGVDSSVVAMLIHKA 239 Query: 258 IGDQLTCMFIDHGLLRKGEAESVMETFVGKFDIHVVKIDARERFLGKLAGVSDPEQKRKI 317 IGDQLTCMFIDHG+LRK EAESVMETFVGKFD+ VVKIDA ERFLGKL GV+DPEQKRKI Sbjct: 240 IGDQLTCMFIDHGMLRKDEAESVMETFVGKFDMKVVKIDASERFLGKLKGVTDPEQKRKI 299 Query: 318 IGNEFIYCFDEESAKLGEFSFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFSLI 377 IGNEFIY FDEESAKLGEF FLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKF L+ Sbjct: 300 IGNEFIYVFDEESAKLGEFDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFELV 359 Query: 378 EPLNTLFKDEVRKLGEELGMPHAIVWRQPFPGPGLAIRVLGEVTEEKLEIVRNSDFILRE 437 EPL TLFKDEVRK+G E G+P IV RQPFPGPGLAIRVLGEVTE+KL IVR SD ILR+ Sbjct: 360 EPLKTLFKDEVRKVGAECGLPDEIVHRQPFPGPGLAIRVLGEVTEDKLHIVRESDAILRD 419 Query: 438 EIAKAGLDREIWQYFTALPNMKSVGVMGDERTYSYTVGIRAVTSIDGMTADWARIPWEVL 497 EIAKAGLDREIWQYFTALP MKSVGVMGD RTYSYTVGIRAVTSIDGMTADWARIPW+VL Sbjct: 420 EIAKAGLDREIWQYFTALPGMKSVGVMGDARTYSYTVGIRAVTSIDGMTADWARIPWDVL 479 Query: 498 EKISXXXXXXXXXXXXXXYDITSKPPATIEWE 529 EKIS YD+TSKPPATIEWE Sbjct: 480 EKISVRIVNEVDNVNRIVYDVTSKPPATIEWE 511 >C0ZK57.1 C0ZK57_BREBN GMP synthase EC=6.3.5.2 Length = 513 Score = 798 bits (2061), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/513 (73%), Positives = 430/513 (83%) Query: 17 VMNKPSEIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPIEKIKALSPKGIVFSGGPS 76 +M+K E++VVLDFGGQYNQLIARR+RDLGVYSEL+P+NTP+EKIK ++PKGI+FSGGP+ Sbjct: 1 MMDKSMEMVVVLDFGGQYNQLIARRVRDLGVYSELIPFNTPVEKIKEMNPKGIIFSGGPA 60 Query: 77 SVYAEDAPHVDPAIYDLGLPIFGICYGMQLMAHQQGGKVERADKREYGKANVDFELDAVL 136 SVY E AP VDPAI+DLGLP+ GICYGMQLM+H GGKVERA KREYGKA + + L Sbjct: 61 SVYEEGAPTVDPAIFDLGLPVLGICYGMQLMSHLTGGKVERAGKREYGKAELRMQQKHGL 120 Query: 137 AAGLESGQTVWMSHGDHVVELPAGFKLDAGTESAPIAAMSHADRKFFAVQFHPEVRHSVN 196 ++ + VWMSH D VVELP GF +DA ++S P+AA+S+ +R+ + VQFHPEV H+ Sbjct: 121 YDKWDTSEIVWMSHSDKVVELPTGFVIDAVSDSCPVAAISNIERQLYGVQFHPEVVHTQK 180 Query: 197 GNEMISNFLYEVCGCEGKWTMESFIEDAVKDIQAKVGDKKVLCALSGGVDSSVVAMLIHR 256 G E I NFL+ VCGCE W+M +FIED + I+ VG+K+VLCALSGGVDSSVVA LIH+ Sbjct: 181 GTEFIGNFLFNVCGCEATWSMTTFIEDELVRIRETVGNKQVLCALSGGVDSSVVAALIHK 240 Query: 257 AIGDQLTCMFIDHGLLRKGEAESVMETFVGKFDIHVVKIDARERFLGKLAGVSDPEQKRK 316 AIGDQLTCMF+DHGLLR+GEAE VMETF KF + V+KIDARERF+ KL GVSDPEQKRK Sbjct: 241 AIGDQLTCMFVDHGLLRQGEAEGVMETFGEKFSMKVIKIDARERFMSKLKGVSDPEQKRK 300 Query: 317 IIGNEFIYCFDEESAKLGEFSFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFSL 376 IIGNEFIY FDEE+AKL + FLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKF+L Sbjct: 301 IIGNEFIYVFDEEAAKLTDMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFTL 360 Query: 377 IEPLNTLFKDEVRKLGEELGMPHAIVWRQPFPGPGLAIRVLGEVTEEKLEIVRNSDFILR 436 IEPL LFKDEVRKLG ELG+P IVWRQPFPGPGL IRVLGEVTEEKL+IVR SD ILR Sbjct: 361 IEPLKALFKDEVRKLGTELGLPDEIVWRQPFPGPGLGIRVLGEVTEEKLKIVRESDAILR 420 Query: 437 EEIAKAGLDREIWQYFTALPNMKSVGVMGDERTYSYTVGIRAVTSIDGMTADWARIPWEV 496 EEIAKAGLDREIWQYFTALPNM +VGVMGD RTYSYTVGIRAVTSIDGMTADWARIPW+V Sbjct: 421 EEIAKAGLDREIWQYFTALPNMTTVGVMGDVRTYSYTVGIRAVTSIDGMTADWARIPWDV 480 Query: 497 LEKISXXXXXXXXXXXXXXYDITSKPPATIEWE 529 LEKIS YD+TSKPPATIEWE Sbjct: 481 LEKISVRIVNEVDNVNRIVYDVTSKPPATIEWE 513 >Q9KF78.1 GUAA_BACHD Putative GMP synthase [glutamine-hydrolyzing] EC=6.3.5.2 Length = 513 Score = 766 bits (1979), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/507 (72%), Positives = 417/507 (82%) Query: 23 EIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPIEKIKALSPKGIVFSGGPSSVYAED 82 E+IVVLDFGGQYNQLI RRIRDLGVYSEL P E++K + PKGI+FSGGP+S YAE Sbjct: 7 EMIVVLDFGGQYNQLITRRIRDLGVYSELHPNTITAEQLKEMKPKGIIFSGGPNSAYAEG 66 Query: 83 APHVDPAIYDLGLPIFGICYGMQLMAHQQGGKVERADKREYGKANVDFELDAVLAAGLES 142 AP DPAI+DLG+PI GICYGMQLM GGKV+ A+ REYGKA + E + L GL Sbjct: 67 APKCDPAIFDLGVPILGICYGMQLMTQHFGGKVDAAEHREYGKATITVENQSKLFQGLPV 126 Query: 143 GQTVWMSHGDHVVELPAGFKLDAGTESAPIAAMSHADRKFFAVQFHPEVRHSVNGNEMIS 202 QTVWMSHGD +V P GF +DA S P+AAMS R ++ VQF PEVRHS G++M+ Sbjct: 127 EQTVWMSHGDLIVAPPEGFVVDAQNPSCPVAAMSDEARNYYGVQFQPEVRHSQFGDDMLK 186 Query: 203 NFLYEVCGCEGKWTMESFIEDAVKDIQAKVGDKKVLCALSGGVDSSVVAMLIHRAIGDQL 262 NF + VCGCEG W+ME+FIE ++ I+ +VGDK+VLCALSGGVDSSVVA+LIH+AIGDQL Sbjct: 187 NFAFAVCGCEGNWSMENFIELEMEKIREQVGDKQVLCALSGGVDSSVVAVLIHKAIGDQL 246 Query: 263 TCMFIDHGLLRKGEAESVMETFVGKFDIHVVKIDARERFLGKLAGVSDPEQKRKIIGNEF 322 TCMFIDHGLLRKGEA+SVM+TF F+++V+KIDA++RFL KL GVSDPEQKRKIIGNEF Sbjct: 247 TCMFIDHGLLRKGEADSVMKTFSEGFNMNVIKIDAKDRFLSKLEGVSDPEQKRKIIGNEF 306 Query: 323 IYCFDEESAKLGEFSFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFSLIEPLNT 382 IY F+EE++KL + FLAQGTLYTDI+ESGTATAQTIKSHHNVGGLPEDM+F LIEPLNT Sbjct: 307 IYVFEEEASKLKDMDFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMRFELIEPLNT 366 Query: 383 LFKDEVRKLGEELGMPHAIVWRQPFPGPGLAIRVLGEVTEEKLEIVRNSDFILREEIAKA 442 LFKDEVRKLG ELG+P +VWRQPFPGPGL IRVLGE+TEEKLEIVR SD ILREEI KA Sbjct: 367 LFKDEVRKLGTELGIPDEVVWRQPFPGPGLGIRVLGEITEEKLEIVRESDAILREEIKKA 426 Query: 443 GLDREIWQYFTALPNMKSVGVMGDERTYSYTVGIRAVTSIDGMTADWARIPWEVLEKISX 502 GLDREIWQYFTALPNM+SVGVMGD RTY YTVGIRAVTSIDGMT+DWARIPW+VLE IS Sbjct: 427 GLDREIWQYFTALPNMRSVGVMGDARTYDYTVGIRAVTSIDGMTSDWARIPWDVLEIIST 486 Query: 503 XXXXXXXXXXXXXYDITSKPPATIEWE 529 YDITSKPPATIEWE Sbjct: 487 RIVNEVKHVNRVVYDITSKPPATIEWE 513 >A7GKG1.1 GUAA_BACCN GMP synthase [glutamine-hydrolyzing] EC=6.3.5.2 Length = 512 Score = 763 bits (1970), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/512 (72%), Positives = 418/512 (81%) Query: 18 MNKPSEIIVVLDFGGQYNQLIARRIRDLGVYSELLPYNTPIEKIKALSPKGIVFSGGPSS 77 M K + I+VLDFG QYNQLIARRIR+ GVYSEL P+ E+IKA++PKGI+FSGGP+S Sbjct: 1 MKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGGPNS 60 Query: 78 VYAEDAPHVDPAIYDLGLPIFGICYGMQLMAHQQGGKVERADKREYGKANVDFELDAVLA 137 VY E+A H D I++LGLPIFGICYGMQLM GGKVERA+ REYGKA + E ++ L Sbjct: 61 VYGENAFHCDEKIFELGLPIFGICYGMQLMTKHFGGKVERANHREYGKAVLKVENESKLY 120 Query: 138 AGLESGQTVWMSHGDHVVELPAGFKLDAGTESAPIAAMSHADRKFFAVQFHPEVRHSVNG 197 L Q VWMSHGD V LP GF +DA +ES PIA MS+ +++ + VQFHPEVRHS +G Sbjct: 121 TNLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAGMSNEEKQLYGVQFHPEVRHSEHG 180 Query: 198 NEMISNFLYEVCGCEGKWTMESFIEDAVKDIQAKVGDKKVLCALSGGVDSSVVAMLIHRA 257 N++I NF++ VCGC W ME+FIE ++ I+ VGDKKVLCALSGGVDSSVVA+LIH+A Sbjct: 181 NDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLIHKA 240 Query: 258 IGDQLTCMFIDHGLLRKGEAESVMETFVGKFDIHVVKIDARERFLGKLAGVSDPEQKRKI 317 IGDQLTC+F+DHGLLRKGEAE VM+TF F ++V+KIDARERF+ KL GV DPEQKRKI Sbjct: 241 IGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKIDARERFMKKLKGVEDPEQKRKI 300 Query: 318 IGNEFIYCFDEESAKLGEFSFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMKFSLI 377 IGNEFIY FD+E+AKL FLAQGTLYTDI+ESGTATAQTIKSHHNVGGLPEDM+F LI Sbjct: 301 IGNEFIYVFDDEAAKLEGIDFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLI 360 Query: 378 EPLNTLFKDEVRKLGEELGMPHAIVWRQPFPGPGLAIRVLGEVTEEKLEIVRNSDFILRE 437 EPLNTLFKDEVR LG ELG+P IVWRQPFPGPGL IRVLGE+TEEKLEIVR SD ILRE Sbjct: 361 EPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGIRVLGEITEEKLEIVRESDAILRE 420 Query: 438 EIAKAGLDREIWQYFTALPNMKSVGVMGDERTYSYTVGIRAVTSIDGMTADWARIPWEVL 497 EI KAGLDREIWQYFTALP M+SVGVMGDERTY YTVGIRAVTSIDGMTADWARIPW+VL Sbjct: 421 EIQKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPWDVL 480 Query: 498 EKISXXXXXXXXXXXXXXYDITSKPPATIEWE 529 EKIS YDITSKPPATIEWE Sbjct: 481 EKISVRIVNEVKHVNRVVYDITSKPPATIEWE 512 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,171,518,967 Number of extensions: 136587135 Number of successful extensions: 362314 Number of sequences better than 1.0e-05: 3664 Number of HSP's gapped: 357578 Number of HSP's successfully gapped: 3712 Length of query: 529 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 388 Effective length of database: 1,732,333,088 Effective search space: 672145238144 Effective search space used: 672145238144 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)