BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0509.0014.p (441 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZI66.1 C0ZI66_BREBN Putative uncharacterized protein 122 9e-26 B1HMS9.1 B1HMS9_LYSSC Putative uncharacterized protein 112 6e-23 B3K601.1 B3K601_9BACI Putative uncharacterized protein 103 4e-20 Q2BC58.1 Q2BC58_9BACI Putative uncharacterized protein 77 5e-12 C0ZI65.1 C0ZI65_BREBN RNA polymerase ECF-type sigma factor 64 3e-08 >C0ZI66.1 C0ZI66_BREBN Putative uncharacterized protein Length = 368 Score = 122 bits (305), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 95/362 (26%), Positives = 173/362 (47%), Gaps = 36/362 (9%) Query: 73 MNPKQLDQELDGLRGDKAEEVPELVRSRMNSVYQELKGSGEKVTPGRRRRSWWQRXXXXX 132 M+ + D EL + K + LVR+R+++ Y L + R R++ + Sbjct: 1 MDQSKFDVELQEYKRTKDSTMSPLVRARLDATYAALPEMSRERKVQRLRKAPYVAAAAVI 60 Query: 133 XXXXXXXXXXXGLGFISPAMAETLKQLPFMKSVFQ-LAGDSGLKQASITGVTSGVQQSVT 191 GFISP MA+++KQ+P + S+F + D GL+ A G++S V +S++ Sbjct: 61 LGATVFTS-----GFISPVMAQSIKQIPLVGSLFSFIEADLGLRAAGEQGLSSKVNRSIS 115 Query: 192 HGDLTLSISELMYDGSRLSLVLTKKESDGGNPSFYEWWNTRDMQLGPA-NNIDFYINGEL 250 H D+ L ++E ++DG+R +L + + F ++L A N+ F I+G+ Sbjct: 116 HHDMKLEVTETVFDGTRAVYLLNVTAPNLKDGMFDTGAEV--VKLSDAIENVTFSIDGKG 173 Query: 251 ANTAWGMAPGG--TASPES-----VIVTALESPNLH----VPEAFNFKMVVFIPDIGQTF 299 + + GG ++ ES V L++P+ + +P++F+ ++VV + I Q F Sbjct: 174 QDDPDSLVTGGFFYSAGESHPNFLVFEEILKTPDHNSTGKIPDSFHAEVVVKLAGIDQAF 233 Query: 300 SFELPVKKKTLESIVLRPEAAKTFDHINLHIKRLEISQTTIRLITEVKGAPGQDIKALQE 359 ++P +K T +I L+P A ++ D I+ + + ++ T RL+ VK K+++ Sbjct: 234 KLDIPFQKATNNTIQLKPNAIQSNDGISFEVSEVNVTPVTTRLLYSVKLDGATSDKSIRA 293 Query: 360 AIPDKYKIYGSLNLLFDLQNDQGQTATMIGGNGNGQGNALSSSSSYEPFAAMPKSVVIKP 419 A LFD DQG+ T + G G + N L+ + Y PK +IKP Sbjct: 294 A-------------LFD---DQGRQLTSLHGEGERKDNTLTFDARYASAQGQPKYFIIKP 337 Query: 420 YI 421 ++ Sbjct: 338 FV 339 >B1HMS9.1 B1HMS9_LYSSC Putative uncharacterized protein Length = 365 Score = 112 bits (281), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 87/317 (27%), Positives = 154/317 (48%), Gaps = 42/317 (13%) Query: 146 GFISPAMAETLKQLPFMKSVFQLAGDSGLKQASITGVTSGVQQSVTHGDLTLSISELMYD 205 G ISP MA +K++P + SVF+ D GL A G+++ + SVTH TL+++E++YD Sbjct: 67 GLISPTMAAAMKEVPLLSSVFKFTRDLGLHAADEKGLSTKLNTSVTHEGFTLNVTEVVYD 126 Query: 206 GSRLSLVLTKKESDGGNPSFYEWWNTRDMQLGPANNIDFYINGE-LANTAWG---MAPGG 261 G+R+++ + +G N+++ +NI+F +NGE L +T + M P Sbjct: 127 GTRVAIGIEGHHKEG--------ENSKESLSDQMSNIEFSLNGEPLNHTNYSGVYMQPSK 178 Query: 262 TASPESVIVTALESPNLHV-PEAFNFKMVVFIPDIGQTFSFELPVKKKTLESIVLRPEAA 320 + + LE+ N + + FN + I I + F ++PV + I L+P + Sbjct: 179 DNNSAVLEFADLENKNGGIFQKKFNLTLSTTITGISEPFKMDIPV-RDIGNYINLQPNIS 237 Query: 321 KTFDHINLHIKRLEISQTTIRLITEVKGAPGQDIKALQEAIPDKYKIYGSLNLLFDLQND 380 + + + I+++ ++ T + T + +E I + ++Y ++ Sbjct: 238 RKYKNNQFTIEQIILTPITTSITTRIALLDKSTSVDFREGI--RIEVY----------DE 285 Query: 381 QGQTATMIGGNGNGQGNALSSSSSYE----PFAAMPKSVVIKPYIWTQD---SSKL---- 429 QG+ +I GNG + N +S + PF +PK++ IKPYI D S +L Sbjct: 286 QGRELKLINGNGGRETNKGNSDFIMDLRVHPFETLPKAITIKPYIHLYDFEGSYQLDENG 345 Query: 430 -----YIPELEMTIPVQ 441 YIPELE+TIP+ Sbjct: 346 DPKIQYIPELEITIPIN 362 >B3K601.1 B3K601_9BACI Putative uncharacterized protein Length = 397 Score = 103 bits (256), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 92/334 (27%), Positives = 153/334 (45%), Gaps = 47/334 (14%) Query: 144 GLGFISPAMAETLKQLPFMKSVFQLAGDSGLKQASITGVTSGVQQSVTHGDLTLSISELM 203 G GF+SP MA TLKQ+P + +++ + +K A G+ S S+TH +TL +++L+ Sbjct: 74 GSGFVSPVMAATLKQIPVLGVLYEGTSEDAVKAAIEQGILSEPNLSLTHDGVTLKVADLL 133 Query: 204 YDGSRLSLVLTKKESD-GGNPSFY-------------EWWNTR-----DMQLGPANNIDF 244 YDG+RLS +L ++ D G S Y E+ +R D + G Sbjct: 134 YDGTRLSFILEREGVDLPGTASPYISEDATLIGNQDSEYVKSRKVAKEDQEKGYIEMPRI 193 Query: 245 YINGELANTAWGMAPGGTASPESVIVTALESPNLHVPEAFNFKMVVFIPDIGQTFSFELP 304 ++G+ G G ++ + L + L +P+ F + + + +TF F++P Sbjct: 194 LVDGQEVAMGEGTF-GDYPQQDNAYIVEL-AKGLKLPDQFELTIQANVTQVNETFEFKIP 251 Query: 305 VKKKTLESIVLRPEAAKTFDH-INLHIKRLEISQTTIRLITEVKGAPGQDIKALQEAIPD 363 V SIV++P+ A D + +K+L+IS + RL+ + KG ++ A E D Sbjct: 252 VNVAN-PSIVVKPDQATKSDGPFSYTVKQLDISPVSTRLVLDSKG----EVPASVEQTGD 306 Query: 364 KYKIYGSLNLLFDLQNDQGQTAT--MIGGNGNGQGNALSSSSSYEPFAAMPKSVVIKPYI 421 Y + + ++L +DQG + + Y P PKS+ IKP+ Sbjct: 307 ----YSASMVYYELVDDQGNVLSPRKFEYFHSEPQTEYHIDQLYSPLNGTPKSITIKPFT 362 Query: 422 WT------------QDS--SKLYIPELEMTIPVQ 441 T QDS K Y+ +LEMTIPV+ Sbjct: 363 LTVNPKDWSVIGQDQDSVGDKTYLKDLEMTIPVK 396 >Q2BC58.1 Q2BC58_9BACI Putative uncharacterized protein Length = 436 Score = 76.6 bits (187), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 79/305 (25%), Positives = 135/305 (44%), Gaps = 44/305 (14%) Query: 144 GLGFISPAMAETLKQLPFMKSVFQLAGDSGLKQASITGVTSGVQQSVTHGDLTLSISELM 203 G SPA+A+ ++P + + F +GD GL+ A G+T V +S T +TL+I+E+ Sbjct: 59 GSAIYSPAIAKIASEIPVVGTFFSGSGDEGLEIAGAKGLTQVVGKSATDKGITLTINEVF 118 Query: 204 YDGSRLSLVLTKKESDGGNPSFYEWWNTRDMQLGPANNIDFYINGELANTAWGMAPGGTA 263 YDG+RL+ T + + +G Y++G N + G + G Sbjct: 119 YDGTRLAFGFTHESL---------------LAIGEMERPAIYVDGNEINFSSG-SSGEFV 162 Query: 264 SPESVIVTALESPNLHVPEAFNFKMVV----FIPDIGQTFSFELPVKKKTLESIVLRPEA 319 +P +P +PE F K V +P + FE PVK+ E V+RP+ Sbjct: 163 TPAKYQGILDINPTEELPEEFTMKFRVDAVGLMPG---KWEFEFPVKQSG-EVTVIRPKE 218 Query: 320 AKTFDHINLHIKRLEISQTTIRLITEVKGAPGQDIKALQEAIPDKYKIYGSLNLLFDLQN 379 K +I+ +E++ ++++ G G D+ A ++ K+ +L F++ + Sbjct: 219 TK-------NIQGMEVNVNSVKI-----GPAGTDLSVSLTADLEQTKLDPYTDLHFNIVD 266 Query: 380 DQGQTATMIGGNGNGQ----GNALSSSSSYEPFAAMPKSVVIKPYIWTQDSSKLYIPELE 435 +QG + G+G+G + S Y P KS+ I PY S PE E Sbjct: 267 NQGLALRQMSGSGHGDTVDGKEEATLSMLYSPVKEGTKSLSIIPYTLPAQHSA---PE-E 322 Query: 436 MTIPV 440 + IP+ Sbjct: 323 VMIPL 327 >C0ZI65.1 C0ZI65_BREBN RNA polymerase ECF-type sigma factor Length = 177 Score = 63.9 bits (154), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 29/59 (49%), Positives = 42/59 (71%) Query: 3 ERMDLRTAVHQLEEIPRTLVILHYYQGFPLQHIADLLEMSEGAVKTRLHRARKTLYEWL 61 E +DLR AV +L+E R +++LHY++ PL+ +A++LE+SE A K RL RAR L E L Sbjct: 106 ELIDLRDAVERLDEQQRLVIVLHYFEDMPLRQVAEILEISESAAKMRLSRARNNLMEKL 164 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,484,226,625 Number of extensions: 99150775 Number of successful extensions: 265435 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 265427 Number of HSP's successfully gapped: 11 Length of query: 441 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 302 Effective length of database: 1,751,175,118 Effective search space: 528854885636 Effective search space used: 528854885636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)