BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0509.0001.p (382 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGN9.1 B3KGN9_9BACI Major facilitator superfamily MFS_1 389 e-106 C0ZKD4.1 C0ZKD4_BREBN Conserved hypothetical membrane protein 306 3e-81 B1DMH4.1 B1DMH4_9BACL Major facilitator superfamily MFS_1 277 1e-72 C2YMF3.1 C2YMF3_BACCE Quinolone resistence protein, major facili... 241 6e-62 B3Z3A6.1 B3Z3A6_BACCE Major facilitator family transporter 241 9e-62 >B3KGN9.1 B3KGN9_9BACI Major facilitator superfamily MFS_1 Length = 412 Score = 389 bits (1000), Expect = e-106, Method: Compositional matrix adjust. Identities = 205/348 (58%), Positives = 263/348 (75%), Gaps = 4/348 (1%) Query: 3 LQMTLSTLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAV 62 LQM L+TLPAYA+ AS +QVSL+TSLFA SAIASR S KA+EKG LL+++GL + Sbjct: 29 LQMMLTTLPAYAKEAFTASPLQVSLITSLFAFSAIASRFVSSKALEKGKLRLLLYVGLLI 88 Query: 63 SITAVIGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTS 122 S+ A +GYYWA I LLL+R+LFGIGFGM ST+FPTMASD+IPI+R+GEGMGYFGLST+ Sbjct: 89 SLAATLGYYWASGIAFLLLMRILFGIGFGMTSTSFPTMASDVIPIKRLGEGMGYFGLSTT 148 Query: 123 LAMSAGPLIGLSLLQGPGFGSLLISTAAALVLILPLSYRLTRKLPAHHKE-PAA--PPVA 179 LAMS GP+IGL+LLQ GF L+ T +LI PL+Y LT + + E PAA P V Sbjct: 149 LAMSIGPMIGLTLLQSGGFTPLVFITVIINLLIFPLAYLLTSRAAKRNAERPAAVQPRVE 208 Query: 180 EIRGGTF-RRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAIVVVRP 238 + G+F ++L +P +LNFL+S++ EAHL + YFFLFNA+AI++VRP Sbjct: 209 DKPKGSFNKKLILPSILNFLMSITYGGLLSFMALFGAEAHLRNPQYFFLFNAIAIILVRP 268 Query: 239 LSGKIYDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQTWM 298 SG+IYDR+G AL+IP ++ +V+GLL L+FA+ST ++ +ALCYGIGFG+MQP++QTWM Sbjct: 269 FSGRIYDRYGHKALVIPAAILMVSGLLLLTFATSTGSMLLSALCYGIGFGAMQPSIQTWM 328 Query: 299 IQSVEPRQRGTANGMFFNSLDLGVAIGTMILGSIALYTSFGVMYRYSA 346 IQ V P QRG ANGMFFNSLD GVAIG++ LG+IA TS+ +MYRYSA Sbjct: 329 IQEVHPLQRGMANGMFFNSLDFGVAIGSIALGAIAKSTSYAIMYRYSA 376 >C0ZKD4.1 C0ZKD4_BREBN Conserved hypothetical membrane protein Length = 402 Score = 306 bits (783), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 167/346 (48%), Positives = 233/346 (67%), Gaps = 4/346 (1%) Query: 3 LQMTLSTLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAV 62 +QM T PAY + T QA+ VSLV SLF+L+A+ +R+F+G+A++ LL + L Sbjct: 27 VQMQTPTFPAYVKETYQANDFVVSLVISLFSLAAVIARVFTGEALKTKSSKLLAIVALGF 86 Query: 63 SITAVIGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTS 122 GYYWAGSI+ LLLR+L GIGFGM ST FPT+ S++IP RR+GEGMGYFGLSTS Sbjct: 87 VAIFSAGYYWAGSIVFFLLLRILVGIGFGMGSTTFPTIVSNVIPARRIGEGMGYFGLSTS 146 Query: 123 LAMSAGPLIGLSLLQGPGFGSLLISTAAALVLILPLSY--RLTRKLPAHHKEPAAPPVAE 180 LAM+ GPLIGL +L G GFGS+L+ + +I PL + R +LP V Sbjct: 147 LAMALGPLIGLGVLNGFGFGSMLMVLVLLVAIIFPLMHFIRAYNQLPVSSGN--TQTVTG 204 Query: 181 IRGGTFRRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAIVVVRPLS 240 IR ++L +P LNF LS++ EAH+E++ +FFL NA+A+V++RP S Sbjct: 205 IRRFYDKKLLLPAGLNFCLSITYGGILSFLALYGKEAHIENVGWFFLTNALAMVLIRPFS 264 Query: 241 GKIYDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQTWMIQ 300 GK+YDR G A+L PG++F+ LL LS ++ + L +A YG+G+G +QP++Q WM++ Sbjct: 265 GKLYDRKGHIAVLPPGAIFVGISLLLLSLTTTESLLLVSAAFYGLGYGMIQPSIQAWMVK 324 Query: 301 SVEPRQRGTANGMFFNSLDLGVAIGTMILGSIALYTSFGVMYRYSA 346 V P QRG ANG+FFNS+DLG+A+G+M+LG IA ++S+ VMYR+SA Sbjct: 325 VVTPEQRGMANGLFFNSIDLGIAVGSMLLGVIATHSSYSVMYRWSA 370 >B1DMH4.1 B1DMH4_9BACL Major facilitator superfamily MFS_1 Length = 411 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 151/371 (40%), Positives = 226/371 (60%), Gaps = 18/371 (4%) Query: 8 STLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAVSITAV 67 S P YA+ ++ +SLVTSLFAL+AI +R + + K RN+++F+GL ++ A Sbjct: 32 SPFPTYAKEQFHPNNFALSLVTSLFALAAIVTRFATAALLRKVHRNVILFVGLVIAAIAT 91 Query: 68 IGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTSLAMSA 127 + Y +A S+ +LL+LR LFGIGFGM ST PT+ S IIP +R GEG+GYFGLSTS+AMS Sbjct: 92 VLYPFASSMTSLLILRALFGIGFGMTSTIIPTLVSQIIPKKRTGEGIGYFGLSTSIAMSL 151 Query: 128 GPLIGLSLLQGPGFGSLLISTAAALVLILPL-----------SYRLTRKLPAHHKEPAAP 176 GP+IGLS+++ GF +L + ALVLI+PL + A EPA+ Sbjct: 152 GPMIGLSVIKQFGFTTLAMIGTIALVLIIPLLIVMRSIPKQPAKAAPPANKAESGEPAS- 210 Query: 177 PVAEIRGGTFRRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAIVVV 236 G + R+ +P +LN L+SV+ + H+E I FFLF + ++ Sbjct: 211 ------GSSTARMMVPAILNVLMSVTYGGILGFIALYGAQKHIEQIGLFFLFIVFTVFII 264 Query: 237 RPLSGKIYDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQT 296 RP+SGK++DR GP ++IPG++ ++A L+ LS+A S + +AL YG GFG++QP Q Sbjct: 265 RPISGKLFDRRGPMPIVIPGAIVVIASLIILSYAESLPLIIVSALLYGFGFGAIQPTTQA 324 Query: 297 WMIQSVEPRQRGTANGMFFNSLDLGVAIGTMILGSIALYTSFGVMYRYSALAPVXXXXXX 356 WM++ P Q AN +++N++D GVA+G+M+LG +A +S+ VMYR++A+A V Sbjct: 325 WMLREAAPHQVSFANSLYYNTIDFGVAVGSMLLGIVASASSYSVMYRWAAVAMVLFMLVY 384 Query: 357 XXXXXAKRYNR 367 +R NR Sbjct: 385 LVSLFIERKNR 395 >C2YMF3.1 C2YMF3_BACCE Quinolone resistence protein, major facilitator family transporter Length = 400 Score = 241 bits (616), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 140/380 (36%), Positives = 214/380 (56%), Gaps = 18/380 (4%) Query: 1 MGLQMTLSTLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGL 60 +G QM + TLP Y + + +S + V +F ++A+ R +G A++K + +++ +G Sbjct: 29 LGFQMLIPTLPVYVK-EIGGTSSNIGFVVGMFTVAALFVRPLTGNALQKFNKKIILMIGT 87 Query: 61 AVSITAVIGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLS 120 A+ + A+ Y +A ++ LL +R+L G GFG+ +T + T+ SD+IP R GEGMGYFGLS Sbjct: 88 AICLLAMGSYLFASTVFLLLAVRILHGAGFGITTTTYGTVVSDLIPQARRGEGMGYFGLS 147 Query: 121 TSLAMSAGPLIGLSLLQGPGFGSLLISTAAALVLILPLSYRLTRKLPAHHKEPAAPPVAE 180 ++AM+ GPLIGL L+Q F L + + V+ L L+ KL K P P + Sbjct: 148 GTIAMALGPLIGLWLMQTYNFTILFLCALSCTVVSLILT-----KLLQIKKSPQPP---Q 199 Query: 181 IRGGTF------RRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAIV 234 GTF R+ +P LL +++ E + I+ FFLFNA+AI Sbjct: 200 QTSGTFLDGFIERKALLPSLLILCITLMYGGIGSFITLFATEVGIADISLFFLFNALAIA 259 Query: 235 VVRPLSGKIYDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPAL 294 V RP SGK+YD G ++IPG + G++ LS+ ++ +L AA CYG GFG++QPAL Sbjct: 260 VTRPFSGKLYDAKGHTFVIIPGVIITFVGIILLSYTTTIPSLIIAAACYGSGFGAIQPAL 319 Query: 295 QTWMIQSVEPRQRGTANGMFFNSLDLGVAIGTMILGSIALYTSFGVMYRYSALAPVXXXX 354 Q WMI V P +RG A FF++ DLG+ G +I G IA +T++ +YRYS+L + Sbjct: 320 QAWMIDRVAPHRRGVATATFFSAFDLGIGAGAIIFGFIAHFTNYATVYRYSSLLLIAFLF 379 Query: 355 XXXXXXXAKRY---NRQKPA 371 ++Y NR+K A Sbjct: 380 IYITSIRKQKYGDKNREKAA 399 >B3Z3A6.1 B3Z3A6_BACCE Major facilitator family transporter Length = 400 Score = 241 bits (615), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 140/381 (36%), Positives = 213/381 (55%), Gaps = 20/381 (5%) Query: 1 MGLQMTLSTLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGL 60 +G QM + TLP Y + + +S + V +F ++A+ R +G A++K + +++ +G Sbjct: 29 LGFQMLIPTLPVYVK-EIGGTSSNIGFVVGMFTVAALFVRPLTGNALQKFSKKIILMIGT 87 Query: 61 AVSITAVIGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLS 120 A+ + A+ Y +A ++ LL +R+L G GFG+ +T + T+ SD+IP R GEGMGYFGLS Sbjct: 88 AICLLAMGSYLFASTVFLLLAVRILHGAGFGITTTTYGTVVSDLIPQARRGEGMGYFGLS 147 Query: 121 TSLAMSAGPLIGLSLLQGPGFGSLLISTAAALVLILPLSYRLTRKLPAHHKEPAAPPVAE 180 ++AM+ GPLIGL L+Q F L + + ++ L L+ KL K P P Sbjct: 148 GTIAMALGPLIGLWLMQTYNFTILFLCALSCTIVSLILT-----KLLQIKKSPQPPKQTS 202 Query: 181 IRGGTF------RRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAIV 234 GTF R+ +P LL +++ E + I+ FFLFNA+AI Sbjct: 203 ---GTFLDGFIERKALLPSLLILCITLMYGGIGSFITLFATEVGIADISLFFLFNALAIA 259 Query: 235 VVRPLSGKIYDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPAL 294 V RP SGK+YD G ++IPG + AG++ LS+ ++ +L AA CYG GFG++QPAL Sbjct: 260 VTRPFSGKLYDAKGHSFVIIPGVIITFAGIILLSYTTTIPSLIIAAACYGSGFGAIQPAL 319 Query: 295 QTWMIQSVEPRQRGTANGMFFNSLDLGVAIGTMILGSIALYTSFGVMYRYSALAPVXXXX 354 Q WMI V P +RG A FF++ DLG+ G +I G IA +T++ +YRYS+L + Sbjct: 320 QAWMIDRVAPHRRGVATATFFSAFDLGIGAGAIIFGFIAHFTNYATVYRYSSLLLIAFLF 379 Query: 355 XXXXXXXAKRYNRQKPAPKNS 375 N+QK KN+ Sbjct: 380 IYITSV-----NKQKHGDKNT 395 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,980,195,336 Number of extensions: 77539306 Number of successful extensions: 360092 Number of sequences better than 1.0e-05: 1353 Number of HSP's gapped: 361494 Number of HSP's successfully gapped: 1390 Length of query: 382 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 245 Effective length of database: 1,770,017,148 Effective search space: 433654201260 Effective search space used: 433654201260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)