BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0012.p (556 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZJ89.1 C0ZJ89_BREBN Putative uncharacterized protein 248 1e-63 B1D5A4.1 B1D5A4_9BACL Extracellular solute-binding protein family 5 224 1e-56 B3KCX6.1 B3KCX6_9BACI Extracellular solute-binding protein family 5 223 5e-56 C0ZGV5.1 C0ZGV5_BREBN Probable ABC transporter substrate binding... 221 1e-55 C2MM05.1 C2MM05_BACCE Oligopeptide-binding protein oppA 218 2e-54 >C0ZJ89.1 C0ZJ89_BREBN Putative uncharacterized protein Length = 631 Score = 248 bits (633), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 183/606 (30%), Positives = 284/606 (46%), Gaps = 61/606 (10%) Query: 7 FLKLHS--HHGGPGE-IPVTLDELAHTLGCTHRNALHIINKMAGLAWITWTPSRGRGKRS 63 FL+L S H G+ PVT+++LA CT R+A I+ K+ L WI W RGRG S Sbjct: 19 FLELRSVFHQEEIGKPFPVTIEKLAGIWHCTPRHAKLIVRKLVELDWIEWKAGRGRGNVS 78 Query: 64 KLTFLADAEEIALESMKQAISSKDVGSAIEGIRGHARSSSLQNTLQGWLLAYFGHHSEIR 123 +T AD++EI L +K I+ DV A+E + + ++++ ++ + WL G S++ Sbjct: 79 VMTLYADSDEILLAEVKLQINKGDVQEAMELMNRYGKAAA-KDQIMDWLSEGMGFSSQLA 137 Query: 124 SNK--QIDTLRLPVNQKLHTFDPLYMNLLAESFVSSHVFDGLVGRS-SERGIVPGLAHAW 180 S D LR PV +++ T DP + ++ + +F+ LV + R I+P +AH+W Sbjct: 138 SQSVSHQDILRFPVYRRIVTLDPGLVYYHFDAHLVGQIFNTLVQYDLNSRSILPSIAHSW 197 Query: 181 DVDGSRTGWTFHLRKEVLFHHGKVLSAEDVVYSLQRLMQTSQRTLYSSIFKEITGVEALN 240 + WTF+L+K VLFHHG+ L+A DVV+SL RL S +F++I + A++ Sbjct: 198 ESSPDARKWTFYLKKNVLFHHGRELTAHDVVFSLNRLRLYPDTYEASWMFRDIDQLIAID 257 Query: 241 STTVRIVLRKPNELFLPFLCTSRAAIVPRDMESGPEGDFGRRPVGTGPFKLVEISEELCV 300 TVRI+L++PN L L L T A+IVP ++ E FG +P+GTGPF+LV ++E +C+ Sbjct: 258 DRTVRILLKEPNYLLLRLLATIPASIVPEEIVWQDEKGFGEKPIGTGPFQLVRLTEGICI 317 Query: 301 LEVFPYYYQGRAHLDRVEILYVPFGIEQ---VQPDTASAF--HVIPNPSTA-------DA 348 LE FP ++ GR LDRVEIL P +E +P+ AS H + + + A D Sbjct: 318 LEAFPAHFLGRPQLDRVEILIFP-ELETGRLKEPNWASVMVSHGVQSKAQALREAVIQDG 376 Query: 349 ASWSRIHSESYVRKFVTCNTKKNGPLSDPQLRA--DILSCLDXXXXXXXXXXXXXXXG-- 404 W + + N K+GP + + R D+L D G Sbjct: 377 QDWCDTEAFFSCCNLLVFNQWKSGPHNHFKFRQALDLLIDRDQMIADLGGDRIYPAKGFR 436 Query: 405 ----------------------------------PLQIATIPQYLGDAGHIASRLIRRGY 430 L++ T + DA I R G Sbjct: 437 TYHPVLREVRARENEQPCHAQIMSLLQESDYQGETLRLVTTAYHEEDALWIQQRCGEYGI 496 Query: 431 ACTVLSVSPEEFKGPVRL-QSDLIVFS--LLRDQDEELRLFDLYQTLAEHVEPGIRSGIE 487 V P EF L + D ++ DQ EL ++ + + I Sbjct: 497 HLAVDVREPWEFSNRDCLPEHDCQLYGNVFSSDQISELEMYLQRNYFLPAFDESLALAIN 556 Query: 488 GMLNLIAREPDPRARAGGFKRIEDKLTQDHQLHILYEKPVQTAYLPSVRGVTFNSQGWVD 547 + L +EPD AR RIE + +++ + L +K ++ S+RGV+ NS GW+D Sbjct: 557 KEIGLTFQEPDRLAREHHLARIEAMIKENYAVLYLVQKRTLASFHKSLRGVSINSSGWLD 616 Query: 548 LRHLWF 553 +WF Sbjct: 617 FHKIWF 622 >B1D5A4.1 B1D5A4_9BACL Extracellular solute-binding protein family 5 Length = 594 Score = 224 bits (572), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 164/578 (28%), Positives = 259/578 (44%), Gaps = 60/578 (10%) Query: 23 TLDELAHTLGCTHRNALHIINKMAGLAWITWTPSRGRGKRSKLTFLADAEEIALESMKQA 82 +L+++A L CT RNA ++ K+ I W RGRG RS++TF D E++ L+ ++ Sbjct: 27 SLEQVAEALFCTERNAKLVLRKLTEEGLIDWQAGRGRGNRSQITFREDREKLLLDLSRRY 86 Query: 83 ISSKDVGSAIEGIRGHARSSSLQNTLQGWLLAYFGHHSEIRSNKQIDTLRLPVNQKLHTF 142 + A ++ + + W+ FG + K+ D LRLP+ + + T Sbjct: 87 AEDGEYNKAFHLLQSYGEGTGANEKFMEWMSGSFGFRRQ--PGKEADVLRLPIFKTIMTL 144 Query: 143 DPLYMNLLAESFVSSHVFDGLVGRSSERG-IVPGLAHAWDVDGSRTGWTFHLRKEVLFHH 201 DP S + +FD LV G I PG+AH W+ + T WTFHLRK +LFH Sbjct: 145 DPADFVYSLCSHMIQQIFDRLVLLDESTGQIKPGIAHYWEHNEDATVWTFHLRKGILFHG 204 Query: 202 GKVLSAEDVVYSLQRLMQTSQRTLYSSIFKEITGVEALNSTTVRIVLRKPNELFLPFLCT 261 G L+A DVV++ +RL +R S I + + VEA++ VR L +PN LFL + + Sbjct: 205 GHELAAADVVFTAERLRNGGKRN--SWIMRSLEQVEAVSERIVRFHLSRPNWLFLKYAAS 262 Query: 262 SRAAIVPRDMESGPEGDFGRRPVGTGPFKLVEISEELCVLEVFPYYYQGRAHLDRVEILY 321 + AI+P +M E F + P GTGPF++ E + +L P+YY RAHLD+VEI++ Sbjct: 263 NVMAILPANMLGLSEEQFWKLPSGTGPFQIEEWKDGKLLLGANPHYYLSRAHLDQVEIVH 322 Query: 322 VPFGIEQVQPDTASAFHVIPNPSTADAA---SWSRIHSESYVRKFVTCNTKKNGPLSDPQ 378 VP E ++ S +I +P+ D W +I S S ++ N KK GP Sbjct: 323 VP-QEEAIKAGMTSWQQLICDPTLRDVTVENGWIQIESRSLCTSLLSWNMKKAGPQRSET 381 Query: 379 LRADILSCLDX-----------------------------------XXXXXXXXXXXXXX 403 R + +D Sbjct: 382 FRRIVNRLIDRHAMIRELSGYRDFPARGFFPELMTVHEPDKDSLEPEEASRLLVEAGYAG 441 Query: 404 GPLQIATIPQYLGDAGHIASRLIRRGYACTVLSVSPEEFKGPVRLQSDL----IVFSLLR 459 P+ + P + D IA+R R G V + P R+ L ++F L Sbjct: 442 EPMSLCAHPGHAEDVDWIANRCRRAGILVEV------RIEAPYRIHHVLDEIDLLFHALV 495 Query: 460 DQDEELRLFDLYQT----LAEHVEPGI-RSGIEGMLNLIAREPDPRARAGGFKRIEDKLT 514 +E++ L + + L E + + R +E +A E DP +R + IE++L Sbjct: 496 FPEEDVSLIEGCEQDGSFLKELMSAELSRWTMESFDQALASE-DPASRKAIYTAIEERLK 554 Query: 515 QDHQLHILYEKPVQTAYLPSVRGVTFNSQGWVDLRHLW 552 + Q+H + T Y PS++GV NS GW+D + ++ Sbjct: 555 GEAQVHFVLHMKFHTDYHPSLKGVRINSLGWIDFKDIF 592 >B3KCX6.1 B3KCX6_9BACI Extracellular solute-binding protein family 5 Length = 543 Score = 223 bits (567), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 133/389 (34%), Positives = 201/389 (51%), Gaps = 17/389 (4%) Query: 3 LHSQFLKLHSHHGGPGEI----PVTLDELAHTLGCTHRNALHIINKMAGLAWITWTPSRG 58 L+ QF LH + VTL+++A TL CT RNA ++ K+ WI W RG Sbjct: 2 LYEQFFMLHRKLAVNAPVHTATEVTLEQIADTLYCTTRNAKLVLRKLEEKGWILWKAGRG 61 Query: 59 RGKRSKLTFLADAEEIALESMKQAISSKDVGSAIEGIRGHARSSSLQNTLQGWLLAYFGH 118 RG RSKLTF AD E+ E+ +Q + A E +R + + ++ W+ +FG Sbjct: 62 RGNRSKLTFQADQAELLQEAAQQLAEKGEYKQAFELLRTYGQGIRTNDSYVEWMNGHFGF 121 Query: 119 HSEIRSNKQI--DTLRLPVNQKLHTFDPLYMNLLAESFVSSHVFDGLVGRSSERG-IVPG 175 E++ ++ D+LRLPV + + T DP ++ + +FD LV + +P Sbjct: 122 CKEMKEGEEEARDSLRLPVYRLIETLDPAECYYSFQAHMIQQIFDRLVVYDKAKDQYMPV 181 Query: 176 LAHAWDVDGSRTGWTFHLRKEVLFHHGKVLSAEDVVYSLQRLMQTSQRTLYSSIFKEITG 235 +AH W+ T WTFHLRK +LFH GK L+AEDV ++++RL Q + I +E+ Sbjct: 182 IAHHWESSEDGTSWTFHLRKGILFHDGKELTAEDVKFTVERLGQDRRNAW---IVRELHS 238 Query: 236 VEALNSTTVRIVLRKPNELFLPFLCTSRAAIVPRDMESGPEGDFGRRPVGTGPFKLVEIS 295 VEA++ VR +L KPN +F+ FL + +IVP+ + E F R PVGTGPF++VE + Sbjct: 239 VEAVSPREVRFILNKPNRIFIRFLSSICMSIVPKHLLIHDEEQFWRLPVGTGPFQVVEWA 298 Query: 296 EELCVLEVFPYYYQGRAHLDRVEILYVPFGIEQVQPDTASAFHVIPNPST----ADAASW 351 EE + +YYQGR HLD ++I+ +P E + S ++ + D + Sbjct: 299 EERLTIHANAHYYQGRPHLDSIQIIMMP---EDAEHCNVSWERLLQDSEQFGHGGDHHAV 355 Query: 352 SRIHSESYVRKFVTCNTKKNGPLSDPQLR 380 I S +T N KK GP + R Sbjct: 356 DIIESSEGCTTLITWNMKKEGPFQSVEFR 384 >C0ZGV5.1 C0ZGV5_BREBN Probable ABC transporter substrate binding protein Length = 601 Score = 221 bits (563), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 173/604 (28%), Positives = 271/604 (44%), Gaps = 58/604 (9%) Query: 1 MKLHSQFLKLHSHHGGPGE-----IPVTLDELAHTLGCTHRNALHIINKMAGLAWITWTP 55 M+L Q+++L + G P E + ++L E+A L CT RNA + KM W+ W P Sbjct: 1 MQLVEQYMRLCA--GRPNETIGHPLDISLAEVASILFCTLRNATLTLKKMQSQGWVVWHP 58 Query: 56 SRGRGKRSKLTFLADAEEIALESMKQAISSKDVGSAIEGIRGHARS-SSLQNTLQGWLLA 114 RGRG RS LT L E++ + K+ + ++ +A + I + + L W+ + Sbjct: 59 GRGRGNRSVLTCLLLPEDLIMSVAKELVQKGEIRAARDMIDEYGGNLPVLGEKFSRWMNS 118 Query: 115 YFGHH--SEIRSNKQIDTLRLPVNQKLHTFDPLYMNLLAESFVSSHVFDGLVGRSS-ERG 171 FGH E S ++DTLRL ++ DP+++ L +ES + +FD LV R Sbjct: 119 QFGHRVKREKGSKGRVDTLRLFYSRPFAGLDPIHILLRSESHMVKQLFDSLVQFDPVTRR 178 Query: 172 IVPGLAHAWDVDGSRTGWTFHLRKEVLFHHGKVLSAEDVVYSLQRLMQTSQRTLYSSIFK 231 I P +A W+ WTF+LRK VLFHHG+ L A+DV +S RLM+ S + + + Sbjct: 179 IEPAIAFYWESSEEGKQWTFYLRKGVLFHHGRPLVADDVRFSFLRLMEHSYKHRW--LAA 236 Query: 232 EITGVEALNSTTVRIVLRKPNELFLPFLCTSRAAIVPRD--MESGPEGDFGRRPVGTGPF 289 I + + + I L P+ELFL L AIVP D +E G + F + PVGTGPF Sbjct: 237 SIESITVKDEYVITISLHMPDELFLQALSKEYMAIVPADYVLEMGEQ--FAQMPVGTGPF 294 Query: 290 KLVEISEELCVLEVFPYYYQGRAHLDRVEILYVPFGIEQVQPDTASAFHV--IPNPSTAD 347 ++V + + VLE F Y+ GR LDR+EI VP + Q S V + + Sbjct: 295 RVVRNDDSMLVLEAFMPYFGGRPFLDRIEIWCVPGMAAEAQAADESLLSVTALDDHRETS 354 Query: 348 AASWSRIHSESYVRKFVTCNTKKNGPLSDPQLRADILSCL-------------------- 387 +WS + ++V+ N KNGPLS + R+ +++ + Sbjct: 355 FLAWSDVGRLEDCFQYVSMNGAKNGPLSHREFRSLVVAIICGQNLRQELQGTREYAVIWG 414 Query: 388 ------------DXXXXXXXXXXXXXXXGPLQIATIP--QYLGDAGHIASRLIRRGYACT 433 L + T P ++ DA I G A Sbjct: 415 KTDSFQEQRKVPGSQEFVRIFKESGYQGEALSLYTYPDSDHIEDAQWIQKTCKAYGIAIE 474 Query: 434 VLSVSPEEFKGPVRLQ-SDLIVFSLLRDQDEELRLFDLYQ----TLAEHVEPGIRSGIEG 488 + SPEE P LQ +D +V S D+ EL L + +++ H++P + I Sbjct: 475 IRYASPEELASPSLLQEADFVVDSANVDERAELSLREFLHAGALSISHHLDPHGKEEIAY 534 Query: 489 MLNLIAREPDPRARAGGFKRIEDKLTQDHQLHILYEKPVQTAYLPSVRGVTFNSQGWVDL 548 +N ++ + R I L + LY V+ P + GV+ ++ GW+D Sbjct: 535 WMNRLSCARTAQDRQACVDSIMHTLAACNTFVPLYSNRVEMLAHPRLSGVSLDAYGWIDF 594 Query: 549 RHLW 552 R ++ Sbjct: 595 RRIF 598 >C2MM05.1 C2MM05_BACCE Oligopeptide-binding protein oppA Length = 579 Score = 218 bits (554), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 173/598 (28%), Positives = 270/598 (45%), Gaps = 67/598 (11%) Query: 1 MKLHSQFLKLHSHHG-GPGE---IPVTLDELAHTLGCTHRNALHIINKMAGLAWITWTPS 56 M + Q+++L +G G E + +T+ ++ TL CT RN+ II K+ L WI W P Sbjct: 2 MFILDQYIELWVAYGKGKKEGEQLEITVQNISETLFCTERNSKLIIKKLDELNWIAWFPG 61 Query: 57 RGRGKRSKLTFLADAEEIALESMKQAISSKDVGSAIEGI-RGHARSSSLQNTLQGWLLAY 115 RGRG+RSK+ F + + L+ K+ DV S IE + R + SS+Q+ Q W+ + Sbjct: 62 RGRGRRSKVIFRKHPKSLILDKGKEITKKGDVRSGIEFVERYSSLFSSVQSEYQSWIDSI 121 Query: 116 FGHHSEIRSNKQIDTLRLPVNQKLH-TFDPLYMNLLAESFVSSHVFDGLVGRSSE-RGIV 173 FGH E+ S + D LRL V L DP+Y + +E + H+FD LV + E I Sbjct: 122 FGHKIEMTSEGRRDVLRLQVQMNLDIALDPVYATMRSECHMVKHIFDTLVYVNEETNSIE 181 Query: 174 PGLAHAWDVDGSRTGWTFHLRKEVLFHHGKVLSAEDVVYSLQRLMQTSQRTLYSSIFKEI 233 P LA W+ D + WTF+LRK V H+GK L+A V++SL+R M+ ++ ++ + + + Sbjct: 182 PRLAFQWEYDDAEKVWTFYLRKGVRLHNGKRLTAHHVIHSLKRFMK-AENNPHAWMLQHV 240 Query: 234 TGVEALNSTTVRIVLRKPNELFLPFLCTSRAAIVPRDMESGPEGDFGRRPVGTGPFKLVE 293 A++ V I L N +FL L + +IV D G+F +GTGPF L E Sbjct: 241 ESFRAVDEFVVEIQLHTENRMFLHMLSAEQCSIVKED----EAGNF----IGTGPFILQE 292 Query: 294 ISEELCVLEVFPYYYQGRAHLDRVEILYVPFGIEQVQPDTASAFHVIPNPSTADAASWSR 353 + L LEV YY+ R LDR+E+L +EQ + + V+ D + ++ Sbjct: 293 KNTHLFALEVHDLYYRERPFLDRIELL----NVEQ----NVNTYDVLVKAQYKDKENLNK 344 Query: 354 IHS--ESYVRKFVTCNTKKNGPLSDPQLRADILSCLDXXXXXXXXXXXXXXXGPLQIATI 411 S ES V +VTCN K GP+ D R + + I Sbjct: 345 ELSRLESNV-TYVTCNLVKKGPMQDELFRKALYKIIHGDKIVQELGGERGEVAKKLILAN 403 Query: 412 PQYLGDAGHIASRLIRRGYACTVLS---------------VSPEEFKGPVRLQS------ 450 + I S + R Y VL + E K +R+++ Sbjct: 404 ESIVDIDEDIQSLIKRSTYHSEVLQLYTFTGRDHVEDAQWIQKECAKYGIRVETNFLEIE 463 Query: 451 DLIVFSLLRDQDEELRLFDLYQTLAEHVEPGI-------RSGIEG--------MLNLIAR 495 DL+ S ++ D + T++E +E + S I G +L+ + Sbjct: 464 DLLEISTIQKAD----MMHDSATISERIEDSLLYMFLTKNSFIHGQSSVDFHKLLSPYFK 519 Query: 496 EPDPRARAGGFKRIEDKLTQDHQLHILYEKPVQTAYLPSVRGVTFNSQGWVDLRHLWF 553 + R + IE+ L + Q+ LY Q V+ + NSQGW+D +WF Sbjct: 520 QEQVEERVTLLRDIEETLLRQVQVIPLYRNKQQVTSHEKVQNIMINSQGWIDFYEIWF 577 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,293,343,354 Number of extensions: 134451121 Number of successful extensions: 297349 Number of sequences better than 1.0e-05: 3719 Number of HSP's gapped: 294551 Number of HSP's successfully gapped: 3719 Length of query: 556 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 415 Effective length of database: 1,732,333,088 Effective search space: 718918231520 Effective search space used: 718918231520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)