BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0006.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7X9.1 B3K7X9_9BACI Cysteine synthase EC=2.5.1.47 424 e-117 C0ZA89.1 C0ZA89_BREBN Probable cysteine synthase EC=2.5.1.47 415 e-114 C3B260.1 C3B260_BACMY Cysteine synthase 404 e-111 B1D7D7.1 B1D7D7_9BACL Cysteine synthase EC=2.5.1.47 404 e-111 C3BIW6.1 C3BIW6_9BACI Cysteine synthase 404 e-111 >B3K7X9.1 B3K7X9_9BACI Cysteine synthase EC=2.5.1.47 Length = 304 Score = 424 bits (1090), Expect = e-117, Method: Compositional matrix adjust. Identities = 207/273 (75%), Positives = 233/273 (85%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLE FNPSGSVKDRAAYNLI AE G+L+PGGTIIEPTSGNTGIGLAM AAAKGY+AI Sbjct: 32 VKLERFNPSGSVKDRAAYNLIHTAEEQGLLQPGGTIIEPTSGNTGIGLAMIAAAKGYRAI 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 L+MPDNM+KERINILKAYGAEVVLTP++ERMPG+IAKALEL +I GSFIPQQFEN ANP Sbjct: 92 LIMPDNMSKERINILKAYGAEVVLTPSSERMPGSIAKALELREQIPGSFIPQQFENAANP 151 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 DIHR+TTAPEI+ QM+GRLD FVA + E LR+ LP + I VVEPQGSPVLS Sbjct: 152 DIHRVTTAPEIMEQMEGRLDAFVATAGTGGTVTGTGEELRKSLPNLHIAVVEPQGSPVLS 211 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GGQPGPHKLVGTSPGFVP ILNT +YDEI+Q++DEDAL+ R LA EGIL+GPS GA+V Sbjct: 212 GGQPGPHKLVGTSPGFVPKILNTSVYDEIIQIADEDALQTARNLARKEGILVGPSSGASV 271 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 + AL+ A+RLG GKRV+CIAPDTGERYLSM IF Sbjct: 272 FAALRVAKRLGEGKRVVCIAPDTGERYLSMDIF 304 >C0ZA89.1 C0ZA89_BREBN Probable cysteine synthase EC=2.5.1.47 Length = 304 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 205/273 (75%), Positives = 232/273 (84%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLE FNPSGSVKDRAAYNLI AE G+++PG TIIEPTSGNTGIGLAMNAAAKGY+AI Sbjct: 32 VKLEKFNPSGSVKDRAAYNLILTAEQAGLIQPGDTIIEPTSGNTGIGLAMNAAAKGYRAI 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 LVMPDNM+KERINILKAYGAEVVLTP+ RMPGAIAKA EL EI SFIPQQFENQANP Sbjct: 92 LVMPDNMSKERINILKAYGAEVVLTPSELRMPGAIAKAQELQKEIPRSFIPQQFENQANP 151 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 DIHR TTA EI AQM+GRLD FVA + EALR++LPE+ I VVEP+GSPVLS Sbjct: 152 DIHRATTAKEIFAQMNGRLDAFVATAGTGGTITGTGEALREKLPELYIAVVEPKGSPVLS 211 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GG+PGPHKLVGTSPGFVP ILNT IY+EI+Q++DEDAL+ +R LA+ EGIL+GPS GA+V Sbjct: 212 GGKPGPHKLVGTSPGFVPQILNTSIYNEIIQIADEDALQSMRQLAALEGILVGPSSGASV 271 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 + A+ A+RLG GKRV+CIAPDTGERYLSM +F Sbjct: 272 FAAITIAKRLGVGKRVVCIAPDTGERYLSMNLF 304 >C3B260.1 C3B260_BACMY Cysteine synthase Length = 305 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/273 (71%), Positives = 229/273 (83%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLEMFNPS SVKDRAAYNLI AE G++KPG TIIEPTSGNTGIGLAMNAAAKGYKAI Sbjct: 32 VKLEMFNPSRSVKDRAAYNLIHMAEEQGLIKPGDTIIEPTSGNTGIGLAMNAAAKGYKAI 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 L+MPDNM+KERIN+LKAYGAEVVLTPA +RMPGAIAKA+EL +I+ SFIPQQFEN ANP Sbjct: 92 LIMPDNMSKERINLLKAYGAEVVLTPAEQRMPGAIAKAVELQKQISNSFIPQQFENPANP 151 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 +IHR TTA EI QMDG+LD FVA + E L+++LP + I VVEP+GSPVLS Sbjct: 152 NIHRYTTALEIYEQMDGKLDAFVATAGTGGTITGTGETLKEKLPNLYIAVVEPKGSPVLS 211 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GG PGPHKLVGTSPGF+P LNT IY+EI+Q++DE+AL +R LA EGIL+GPS GA+V Sbjct: 212 GGVPGPHKLVGTSPGFIPKNLNTEIYNEIIQIADEEALSTMRNLAKQEGILVGPSSGASV 271 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 + A+ A++LG+GK+VLCIAPDTGERYLSMG+F Sbjct: 272 YAAITIAKQLGAGKKVLCIAPDTGERYLSMGLF 304 >B1D7D7.1 B1D7D7_9BACL Cysteine synthase EC=2.5.1.47 Length = 305 Score = 404 bits (1038), Expect = e-111, Method: Compositional matrix adjust. Identities = 201/273 (73%), Positives = 222/273 (81%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLE FNPSGSVKDRAA LI AE G++ PG TIIEPTSGNTGIGLAMNAAAKGYK I Sbjct: 33 VKLERFNPSGSVKDRAASALIEAAEQSGLISPGCTIIEPTSGNTGIGLAMNAAAKGYKLI 92 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 L+MPDNM+ ERI +LKAYGA+VVLTPAAERMPGAI KALEL EI GSFIP QFEN ANP Sbjct: 93 LIMPDNMSAERIKLLKAYGADVVLTPAAERMPGAIRKALELQAEIPGSFIPNQFENHANP 152 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 DIHR TTA EIL QM G LD FVA + E L+ LP I I VVEP GSPVLS Sbjct: 153 DIHRTTTAVEILRQMGGMLDAFVATAGTGGTITGTGETLKASLPHIHIAVVEPAGSPVLS 212 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GGQPGPHKLVGTSPGFVPAILNT +YDEI+QV+DEDA++ +R LA EG+L+GPS GA+V Sbjct: 213 GGQPGPHKLVGTSPGFVPAILNTEVYDEIIQVADEDAIQTMRDLARLEGLLLGPSSGASV 272 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 W A+Q A++LG+GKRVLCIAPDTGERYLSM IF Sbjct: 273 WAAMQVAKKLGAGKRVLCIAPDTGERYLSMNIF 305 >C3BIW6.1 C3BIW6_9BACI Cysteine synthase Length = 305 Score = 404 bits (1037), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/273 (71%), Positives = 229/273 (83%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLEMFNPS SVKDRAAYNLI AE G++KPG TIIEPTSGNTGIGLAMNAAAKGYKAI Sbjct: 32 VKLEMFNPSRSVKDRAAYNLIHMAEEQGLIKPGDTIIEPTSGNTGIGLAMNAAAKGYKAI 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 L+MPDNM+KERIN+LKAYGAEVVLTPA +RMPGAIAKA+EL +I+ SFIPQQFEN ANP Sbjct: 92 LIMPDNMSKERINLLKAYGAEVVLTPAEQRMPGAIAKAVELQKQISNSFIPQQFENPANP 151 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 +IHR TTA EI QMDG+LD FVA + E L+++LP + I VVEP+GSPVLS Sbjct: 152 NIHRYTTALEIYEQMDGKLDAFVATAGTGGTITGTGEILKEKLPNLYIAVVEPKGSPVLS 211 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GG PGPHKLVGTSPGF+P LNT IY+EI+Q++DE+AL +R LA EGIL+GPS GA+V Sbjct: 212 GGVPGPHKLVGTSPGFIPKNLNTEIYNEIIQIADEEALSTMRNLAKQEGILVGPSSGASV 271 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 + A+ A++LG+GK+VLCIAPDTGERYLSMG+F Sbjct: 272 YAAITIAKQLGAGKKVLCIAPDTGERYLSMGLF 304 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,656,467,229 Number of extensions: 68606480 Number of successful extensions: 215903 Number of sequences better than 1.0e-05: 4186 Number of HSP's gapped: 209696 Number of HSP's successfully gapped: 4201 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)