BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0004.p (265 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KCV7.1 B3KCV7_9BACI Histidine kinase dimerisation and phosphoa... 204 6e-51 C0ZDW3.1 C0ZDW3_BREBN Probable two-component sensor histidine ki... 180 1e-43 B1D7E9.1 B1D7E9_9BACL Integral membrane sensor signal transducti... 165 4e-39 C0ZBE0.1 C0ZBE0_BREBN Two-component sensor histidine kinase EC=2... 127 9e-28 Q5P3L3.1 Q5P3L3_AZOSE Nitrate/nitrite TWO-component sensor NARX 92 3e-17 >B3KCV7.1 B3KCV7_9BACI Histidine kinase dimerisation and phosphoacceptor region Length = 275 Score = 204 bits (519), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 103/263 (39%), Positives = 153/263 (58%), Gaps = 5/263 (1%) Query: 2 VGIWEIVRHQFLMPYLSMDMGNYLTXXXXXXXXXXXXXXXXXXMEKNSRELEQERLLKTA 61 +GIWE VRH+FL+PY+SMD+GN L +E+N EL + R L+ A Sbjct: 18 IGIWEYVRHEFLLPYISMDLGNLLAPVIVFLVSILFLTQLFSLIERNQEELNRSRALQAA 77 Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETIGQLKKNVHETNRYVRQA 121 M RE + +ELHDGIAQSLFLL+ +IDR+E+ G++ + ++K++VH TN YVR+A Sbjct: 78 MHEREKIGRELHDGIAQSLFLLNAQIDRIEKSGRTGDIPYQ---EIKESVHRTNTYVREA 134 Query: 122 ISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIRE 181 I+NL+ P A Q S+ + +I +E + + W + D LS E+ ELL+ IRE Sbjct: 135 IANLRYP-ADPQAIPWMHSMSSLIEEIKQETDLHFDIKWGIPDAMLSAKEKVELLASIRE 193 Query: 182 AMINVRKHTRAGRVAISGREDGQGWRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAEGM 241 +++N+ KH A V I+ GW ++DDG G + P S YG++IMK+RAE M Sbjct: 194 SLLNIHKHAEAKHVKITANTTDSGWHCTVTDDGKGFDLDKPLG-SNRYGIKIMKDRAEAM 252 Query: 242 GWTLALKSGDYGTIVEIAKKGAS 264 GW ++ TI+ I K+ + Sbjct: 253 GWKFQMERQHDQTIINIRKESTA 275 >C0ZDW3.1 C0ZDW3_BREBN Probable two-component sensor histidine kinase EC=2.7.3.- Length = 313 Score = 180 bits (457), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 96/261 (36%), Positives = 148/261 (56%), Gaps = 5/261 (1%) Query: 2 VGIWEIVRHQFLMPYLSMDMGNYLTXXXXXXXXXXXXXXXXXXMEKNSRELEQERLLKTA 61 +G WE VRH+FL+PY+SM++GN+L +E N EL + + ++ Sbjct: 57 IGTWEYVRHEFLLPYISMELGNWLAPIIVLVVSLLFLTQLFAMIEHNHEELNRSKAVQAI 116 Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETIGQLKKNVHETNRYVRQA 121 +E RE +A+ELHDGIAQSLF L+ ++ R+E+ ++D I + KK+VH TN YVRQA Sbjct: 117 LEEREQIARELHDGIAQSLFFLNAQVSRIERM---KQVDELLIDKFKKSVHHTNDYVRQA 173 Query: 122 ISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIRE 181 I+NL+ + L Q V+ + ++ RE ++ +W + + LS ++ ELL+ +RE Sbjct: 174 IANLRYAPDVNRIPWL-QGVESLIDELRRETNMQFETEWEIPESLLSTKDKVELLAIVRE 232 Query: 182 AMINVRKHTRAGRVAISGREDGQGWRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAEGM 241 A++N+ KH A V + + GW I DDG G E S SYGL++M++RA M Sbjct: 233 ALLNIHKHANATCVRVHALANKNGWICQIIDDGKGFDLEKEMGGS-SYGLKMMRDRANNM 291 Query: 242 GWTLALKSGDYGTIVEIAKKG 262 W L + D T+V I K+ Sbjct: 292 SWNLNYERKDGETMVTILKEA 312 >B1D7E9.1 B1D7E9_9BACL Integral membrane sensor signal transduction histidine kinase Length = 281 Score = 165 bits (417), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 99/266 (37%), Positives = 149/266 (56%), Gaps = 9/266 (3%) Query: 1 MVGIWEIVRHQFLMPYLSMDMGNYLTXXXXXXXXXXXXXXXXXXMEKNSRELEQERLLKT 60 +VG+WE VRH FL+PY+SM++GN+L +E+ L++ER+ K Sbjct: 17 VVGLWEYVRHVFLLPYISMELGNFLAPALVFLFTLTLLRGLFGKLEQTQEALQRERVSKA 76 Query: 61 AMEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETIGQLKKNVHETNRYVRQ 120 + + RE LA+ELHDGI+QSLFLLSV++D+LE E G +T Q+++ V VRQ Sbjct: 77 SFQQREQLARELHDGISQSLFLLSVKLDKLEHM-ELGNDARDTTVQIRQTVKHVYEDVRQ 135 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 +I+NL+ P S L ++ + + + V +DW L + LS + EL S IR Sbjct: 136 SIANLQSPAVVVDDSWLT-AIHGAVNDLLQNSGVRAKLDWQLPEGVLSNKQLVELQSIIR 194 Query: 181 EAMINVRKHTRAGRVAISGREDG-QGWRVAISDDGAGIRHEDPFAVSGS--YGLQIMKER 237 EA++NVRK+ +A V + R+ G + + I D+G G DP + YG+++M++R Sbjct: 195 EALMNVRKYAQATEVQVISRQHGDEDFVCEIKDNGVG---ADPSQIDAKDRYGIRMMRDR 251 Query: 238 AEGMGWTLALKSG-DYGTIVEIAKKG 262 AE MGW +KS GT V I G Sbjct: 252 AEQMGWLFDIKSAVGEGTTVRIQSGG 277 >C0ZBE0.1 C0ZBE0_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 278 Score = 127 bits (320), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 84/241 (34%), Positives = 132/241 (54%), Gaps = 22/241 (9%) Query: 1 MVGIWEIVRHQFLMPYLSMDMGN-YLTXXXXXXXXXXXXXXXXXXMEKNSRELEQERLLK 59 ++G +E +RH FL+PY+SM+ GN Y+T + N+R +E E+ + Sbjct: 27 IIGGFEFIRHDFLLPYMSMEAGNVYITLLTLLLSFLFATWMFHTLKQMNARIIE-EQARR 85 Query: 60 TAMEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETIGQLKKNVHETNRYVR 119 E RE LA+ELHDGIAQSLF L+V++ ++G +D I V + +VR Sbjct: 86 AVYEERERLARELHDGIAQSLFFLNVKL-------KQGHLDDARIA-----VSAIDNHVR 133 Query: 120 QAISNLK-VPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSC 178 QAI NL+ +P G+ L Q +++ L Q + ++VA + ++D +P + +L Sbjct: 134 QAIFNLRSLPEEGS----LDQRLEKWLAQWSALSGIDVASELHVKDGFFTPTAEVQLFGI 189 Query: 179 IREAMINVRKHTRAGRVAISGRED-GQGWRVAISDDGAGIRHEDPFAVSGSYGLQIMKER 237 I+EA N+RKH++A I D GW +++ DDG GI +PF + YGL +M+ER Sbjct: 190 IQEAFANIRKHSQAKHSWIHLTTDEPTGWVLSVEDDGIGI--SNPFPDTKKYGLSMMRER 247 Query: 238 A 238 A Sbjct: 248 A 248 >Q5P3L3.1 Q5P3L3_AZOSE Nitrate/nitrite TWO-component sensor NARX Length = 648 Score = 92.4 bits (228), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 73/219 (33%), Positives = 113/219 (51%), Gaps = 20/219 (9%) Query: 49 SRELEQERLLKTAMEAREALAKELHDGIAQSLFLLSVRIDRLE---QKHERGEMDAETIG 105 S + E+ERLL E R +A+ELHD +AQSL + ++ L Q+ ER + ++ + Sbjct: 421 SHQSERERLLAL-QEERSIIARELHDSLAQSLSYMKIQASLLSSALQRPERRDDASDILA 479 Query: 106 QLKKNVHETNRYVRQAISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDE 165 +K ++ R +R+ +S ++ + G LL +V+E + + +E+ L Sbjct: 480 DMKGGINAAYRQLRELLSTFRLQMEGDFARLLGSTVEEYAGRSGMPIDLEI----RLGGR 535 Query: 166 NLSPAEQAELLSCIREAMINVRKHTRAG--RVAISGREDGQGWRVAISDDGAGIR---HE 220 +L+P ++ +L IREA+ N +H RA RV + G DG RV I DDG GIR Sbjct: 536 HLNPNQEIHVLHIIREALSNATRHARASSIRVGLFGHPDG--VRVIIEDDGKGIRAAAAT 593 Query: 221 DPFAVSGSYGLQIMKERAEGMGWTLAL-KSGDYGTIVEI 258 +P YGL IM ERA G+G L + D GT V + Sbjct: 594 EPH----HYGLAIMGERARGLGGVLEIVPRPDGGTRVAV 628 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.130 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,334,235,625 Number of extensions: 48430419 Number of successful extensions: 162215 Number of sequences better than 1.0e-05: 1712 Number of HSP's gapped: 161740 Number of HSP's successfully gapped: 1712 Length of query: 265 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 132 Effective length of database: 1,807,701,208 Effective search space: 238616559456 Effective search space used: 238616559456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)