BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0060.p (180 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIL7.1 B3KIL7_9BACI Isochorismatase hydrolase 293 5e-78 Q2BAM8.1 Q2BAM8_9BACI Pyrazinamidase 259 7e-68 A8VXZ9.1 A8VXZ9_9BACI Amidase-like protein 253 5e-66 C1P9J7.1 C1P9J7_BACCO Isochorismatase hydrolase 252 1e-65 A8FGY3.1 A8FGY3_BACP2 Nicotinamidase EC=3.5.1.19 251 1e-65 >B3KIL7.1 B3KIL7_9BACI Isochorismatase hydrolase Length = 185 Score = 293 bits (750), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 135/180 (75%), Positives = 153/180 (85%) Query: 1 MRALIVIDFTNDFVDGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYHP 60 M+ALIVIDFTNDFVDG+LPVG+PAIDI I+ LT+ + D GD+VVMAVDLHE++D +HP Sbjct: 1 MKALIVIDFTNDFVDGNLPVGKPAIDIQKTIAELTRLYSDRGDFVVMAVDLHEQDDAFHP 60 Query: 61 EAKLFPPHNLRGTAGRELYGSLKDVYEQNRDSIYWMDKTRYSAFCGTDLTLKLRERGITE 120 E KLFPPHN+RGT GRELYG L+ VYE +D+IYWMDKTRYSAFCGTDL KLRERGITE Sbjct: 61 ETKLFPPHNIRGTRGRELYGELQQVYEDRKDTIYWMDKTRYSAFCGTDLNQKLRERGITE 120 Query: 121 LHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVVRVE 180 + L GVCTDICVLHTAVDAYN G+ I V++D VASFNPEGH WAL HF GSLGA VVR E Sbjct: 121 VDLVGVCTDICVLHTAVDAYNYGYKITVYEDGVASFNPEGHKWALSHFQGSLGAAVVRSE 180 >Q2BAM8.1 Q2BAM8_9BACI Pyrazinamidase Length = 181 Score = 259 bits (662), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 118/179 (65%), Positives = 145/179 (81%), Gaps = 2/179 (1%) Query: 2 RALIVIDFTNDFVDGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYHPE 61 +ALI ID+T DFV G+LP G+PA +I I+ LT+ F+ G Y V A+DLH+ENDP+HPE Sbjct: 3 KALINIDYTYDFVIGALPCGKPAQEIEEYITNLTETFIKEGHYTVFAIDLHKENDPFHPE 62 Query: 62 AKLFPPHNLRGTAGRELYGSLKDVY--EQNRDSIYWMDKTRYSAFCGTDLTLKLRERGIT 119 +L+PPHN+ GT GR+LYGSLK+V+ Q++++++WMDKTRYSAF GTDL LKLRERGI Sbjct: 63 TELYPPHNIEGTKGRDLYGSLKNVFMQHQSKENVHWMDKTRYSAFAGTDLELKLRERGIE 122 Query: 120 ELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVVR 178 ELHLAGVCTDICVLHTAVDAYNKG+ I+VH+ AVASFN GH WAL HF GSL A V+ Sbjct: 123 ELHLAGVCTDICVLHTAVDAYNKGYKIIVHEKAVASFNQAGHSWALEHFKGSLNALVIE 181 >A8VXZ9.1 A8VXZ9_9BACI Amidase-like protein Length = 188 Score = 253 bits (646), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 122/183 (66%), Positives = 145/183 (79%), Gaps = 6/183 (3%) Query: 1 MRALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPY 58 M+AL+VID+TNDFV DG L G+ I RI+ LT++F D G YVVMAVD HE+ D Y Sbjct: 1 MKALLVIDYTNDFVATDGKLTCGERGQSIEARITALTKSFFDHGHYVVMAVDSHEKEDRY 60 Query: 59 HPEAKLFPPHNLRGTAGRELYGSLKDVYEQNR----DSIYWMDKTRYSAFCGTDLTLKLR 114 HPE LFP HN+RGT GR LYG+L D+YE+ R ++YWMDKTRYSAF GTDL LKLR Sbjct: 61 HPEFPLFPEHNVRGTDGRTLYGTLGDLYERIRKGTPSNLYWMDKTRYSAFAGTDLELKLR 120 Query: 115 ERGITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGA 174 ERGI+ELH++GVCTDICVLHTAVDA+NKGFSIVVH+DAV SF+P+GH WAL HF +LGA Sbjct: 121 ERGISELHVSGVCTDICVLHTAVDAFNKGFSIVVHEDAVESFDPQGHDWALSHFQNTLGA 180 Query: 175 KVV 177 + V Sbjct: 181 QTV 183 >C1P9J7.1 C1P9J7_BACCO Isochorismatase hydrolase Length = 184 Score = 252 bits (643), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 117/179 (65%), Positives = 146/179 (81%), Gaps = 4/179 (2%) Query: 2 RALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYH 59 +ALI ID+TNDFV DG L G+P I I LT++F+++GDYVV A+D HE++DPYH Sbjct: 5 KALINIDYTNDFVADDGRLTCGKPGQQIEENIVALTKSFLENGDYVVFAIDCHEQDDPYH 64 Query: 60 PEAKLFPPHNLRGTAGRELYGSLKDVYE--QNRDSIYWMDKTRYSAFCGTDLTLKLRERG 117 PE KLFPPHNL+GT GR+LYG L+ ++ + + +++ +DKTRYSAFCGTDL L+LRERG Sbjct: 65 PETKLFPPHNLKGTPGRDLYGKLQPLFTAWKGKKNVHVIDKTRYSAFCGTDLELRLRERG 124 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKV 176 I E+HL GVCTDICVLHTAVDAYNKGF +VVH++AVASFNP+GH WAL HF GSLGA+V Sbjct: 125 IEEIHLCGVCTDICVLHTAVDAYNKGFKLVVHENAVASFNPDGHKWALSHFKGSLGAEV 183 >A8FGY3.1 A8FGY3_BACP2 Nicotinamidase EC=3.5.1.19 Length = 183 Score = 251 bits (642), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 118/181 (65%), Positives = 141/181 (77%), Gaps = 4/181 (2%) Query: 2 RALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYH 59 +ALI ID+T DFV DG L G+P I P+I+ +T +F+D G +VV AVD HEE DPYH Sbjct: 3 KALICIDYTVDFVADDGKLTCGKPGQAIEPKITEITSSFIDEGHFVVFAVDHHEEQDPYH 62 Query: 60 PEAKLFPPHNLRGTAGRELYGSLKDVYEQNR--DSIYWMDKTRYSAFCGTDLTLKLRERG 117 PE KLFPPHN+RGT G ELYG L ++ ++ +Y+M+KTRYSAF GT L +KLRERG Sbjct: 63 PETKLFPPHNIRGTKGIELYGKLSSLFHTSKHLKHVYYMEKTRYSAFAGTQLEMKLRERG 122 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVV 177 ITELHLAGVCTDICVLHTAVDAYNKGF +V+H++AVASFN GH WAL HF SLGAKVV Sbjct: 123 ITELHLAGVCTDICVLHTAVDAYNKGFELVIHENAVASFNEAGHEWALSHFEQSLGAKVV 182 Query: 178 R 178 + Sbjct: 183 K 183 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,163,806,941 Number of extensions: 47893075 Number of successful extensions: 101411 Number of sequences better than 1.0e-05: 1059 Number of HSP's gapped: 100612 Number of HSP's successfully gapped: 1059 Length of query: 180 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 53 Effective length of database: 1,864,227,298 Effective search space: 98804046794 Effective search space used: 98804046794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)